Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,134,394	A→G	D187G (GAC→GGC)	katG →	catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,134,394	0	A	G	100.0%	172.7 / NA	46	D187G (GAC→GGC)	katG	catalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

CGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGG  >  NC_000913/4134251‑4134541                                                                                                                                                |                                                                                                                                                    cGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCt                                                                                                                                                <  2:580261/149‑1 (MQ=255) cGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTgg                                                                                                                                              <  2:485784/151‑1 (MQ=255)  gATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTggg                                                                                                                                             >  2:239484/1‑151 (MQ=255)     aaaGCGCGTCGACTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCACGGGTAACGAGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAAc                                                                                                                                          >  1:537659/1‑151 (MQ=255)             tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCt                                                                                                                                                <  1:106274/137‑1 (MQ=255)             tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCt                                                                                                                                                >  2:106275/1‑137 (MQ=255)                ccTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGAt                                                                                                                               <  1:534547/151‑1 (MQ=255)                        ggCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTg                                                                                                                       <  2:256352/151‑1 (MQ=255)                         gCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTgg                                                                                                                      <  2:378680/151‑1 (MQ=255)                               cAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGtga                                                                                                                 <  1:506268/150‑1 (MQ=255)                                aaaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGtgatg                                                                                                               >  2:487198/1‑151 (MQ=255)                                 aaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGtgatg                                                                                                               >  2:667587/1‑150 (MQ=255)                                  aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaa                                                                                                             <  1:588180/151‑1 (MQ=255)                                     gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaaaaa                                                                                                          <  1:461794/151‑1 (MQ=255)                                     gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaaaaa                                                                                                          <  2:159491/151‑1 (MQ=255)                                      aaaTATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAg                                                                                                         <  2:682481/151‑1 (MQ=255)                                          atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCt                                                                                                      <  2:41630/150‑1 (MQ=255)                                                        tcCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTc                                                                                       >  1:463825/1‑151 (MQ=255)                                                         ccTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTc                                                                                       >  2:401250/1‑150 (MQ=255)                                                         ccTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCa                                                                                      <  2:494533/151‑1 (MQ=255)                                                           tGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATc                                                                                    >  1:193083/1‑151 (MQ=255)                                                             ggCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCg                                                                                  <  1:576153/151‑1 (MQ=255)                                                             ggCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCg                                                                                  <  1:128489/151‑1 (MQ=255)                                                                  aCCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAgc                                                                             <  2:234376/151‑1 (MQ=255)                                                                              tCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGc                                                                 >  2:545596/1‑151 (MQ=255)                                                                               cgcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGc                                                                 >  2:203745/1‑150 (MQ=255)                                                                               cgcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCa                                                                <  1:33799/151‑1 (MQ=255)                                                                                        cGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTggg                                                        >  2:358311/1‑150 (MQ=255)                                                                                                    aaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGGGATg                                           <  1:25723/151‑1 (MQ=255)                                                                                                       cTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGCGATGGGt                                        >  1:571368/1‑151 (MQ=255)                                                                                                        tCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTc                                       >  2:215932/1‑151 (MQ=255)                                                                                                         ccGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTc                                       <  1:203445/150‑1 (MQ=255)                                                                                                           ggCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTg                                     >  2:178810/1‑150 (MQ=255)                                                                                                               tCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAttt                                 >  2:72677/1‑150 (MQ=255)                                                                                                                    aCCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTa                           <  1:603874/151‑1 (MQ=255)                                                                                                                       ttCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAcc                        >  2:633149/1‑151 (MQ=255)                                                                                                                         cGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                      <  2:401433/151‑1 (MQ=255)                                                                                                                         cGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                      <  2:438435/151‑1 (MQ=255)                                                                                                                          ggTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCgg                     >  2:477926/1‑151 (MQ=255)                                                                                                                                  tGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCg             >  1:575808/1‑151 (MQ=255)                                                                                                                                   gCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCgg            >  1:374465/1‑151 (MQ=255)                                                                                                                                   gCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCg             <  2:249521/150‑1 (MQ=255)                                                                                                                                      ggTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATc         >  1:102022/1‑151 (MQ=255)                                                                                                                                            gAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg   >  1:579330/1‑151 (MQ=255)                                                                                                                                            gAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg   <  2:101999/151‑1 (MQ=255)                                                                                                                                             aaGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcgg  <  2:620847/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    CGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGG  >  NC_000913/4134251‑4134541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑