Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,255,504 G→A H321H (CAC→CAT plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,255,5040GA100.0% 190.9 / NA 49H321H (CAC→CATplsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/17);  total (32/17)

GATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA  >  NC_000913/4255359‑4255650
                                                                                                                                                 |                                                                                                                                                  
gaTACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTgatgat                                                                                                                                                >  2:349152/1‑150 (MQ=255)
   aCTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGCTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATAc                                                                                                                                              >  1:518015/1‑149 (MQ=255)
    cTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGc                                                                                                                                           >  1:126137/1‑151 (MQ=255)
     tCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGc                                                                                                                                           >  2:687634/1‑150 (MQ=255)
             aaCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGa                                                                                                                                   >  2:508670/1‑150 (MQ=255)
             aaCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGa                                                                                                                                   >  1:633138/1‑150 (MQ=255)
              aCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGaa                                                                                                                                  >  2:515319/1‑150 (MQ=255)
               cGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGa                                                                                                                                   >  1:629377/1‑148 (MQ=255)
                ggTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAagc                                                                                                                               <  2:475587/151‑1 (MQ=255)
                   ggAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAagcagc                                                                                                                            >  2:598138/1‑151 (MQ=255)
                         aaGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAg                                                                                                                      <  2:458287/151‑1 (MQ=255)
                             ttATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTcc                                                                                                                   >  2:568759/1‑150 (MQ=255)
                               aTTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtg                                                                                                                <  1:560319/151‑1 (MQ=255)
                                ttGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtgt                                                                                                               >  2:3793/1‑151 (MQ=255)
                                  gCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtgtg                                                                                                              <  2:590656/150‑1 (MQ=255)
                                      ttAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACt                                                                                                           <  2:325118/149‑1 (MQ=255)
                                       tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCg                                                                                                        >  2:537132/1‑151 (MQ=255)
                                       tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCg                                                                                                        >  1:322201/1‑151 (MQ=255)
                                        aaaCGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCgg                                                                                                       <  2:209911/151‑1 (MQ=255)
                                            gTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGc                                                                                                   <  1:388488/151‑1 (MQ=255)
                                            gTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGc                                                                                                   <  2:369364/151‑1 (MQ=255)
                                               cGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCa                                                                                                  >  1:283903/1‑149 (MQ=255)
                                                 gCGAATAAAGAACACCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGAACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAgg                                                                                               >  1:42874/1‑150 (MQ=255)
                                                      tAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACat                                                                                          >  1:624631/1‑150 (MQ=255)
                                                         aGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATAc                                                                                      >  1:174858/1‑151 (MQ=255)
                                                           aaCGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATAcc                                                                                     >  2:219557/1‑150 (MQ=255)
                                                             cGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGc                                                                                  >  2:249318/1‑151 (MQ=255)
                                                                     gACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGAcc                                                                          <  2:610681/151‑1 (MQ=255)
                                                                              aaaTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGc                                                                 >  2:615844/1‑151 (MQ=255)
                                                                                   ggCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCt                                                            <  1:263377/151‑1 (MQ=255)
                                                                                     cccGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTgg                                                          >  1:173063/1‑151 (MQ=255)
                                                                                          cAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAAc                                                     <  2:433955/151‑1 (MQ=255)
                                                                                              ccAGAAATTCAGGTTGATCCCGGCGGCGATAGGCGGCGGCACCAGCTCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCATCTGGCGAACgcgc                                                 <  1:125940/151‑1 (MQ=255)
                                                                                                    aTTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGCGGGCCAGCTGGCGAACGCGCTCAGCg                                           <  1:602598/151‑1 (MQ=255)
                                                                                                           ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCACTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGCGGGCCAGCTGGCGAACGCGCTCAGCGTTATgg                                     >  1:536761/1‑150 (MQ=255)
                                                                                                              aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGt                                 >  2:207664/1‑151 (MQ=255)
                                                                                                                   ggcggcGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATg                            >  2:511554/1‑151 (MQ=255)
                                                                                                                   ggcggcGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATg                            >  1:346768/1‑151 (MQ=255)
                                                                                                                      ggcgATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGccc                         >  2:239737/1‑151 (MQ=255)
                                                                                                                       gcgATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCt                        >  2:267728/1‑151 (MQ=255)
                                                                                                                          atatGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGt                     >  2:453045/1‑151 (MQ=255)
                                                                                                                            atGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTa                    <  2:498301/150‑1 (MQ=255)
                                                                                                                                    gcACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGtt           >  2:51804/1‑151 (MQ=255)
                                                                                                                                      aCCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc         <  1:619928/151‑1 (MQ=255)
                                                                                                                                       ccAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc         <  1:393081/150‑1 (MQ=255)
                                                                                                                                        cAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc         >  1:343135/1‑149 (MQ=255)
                                                                                                                                         aGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAgg      >  2:574155/1‑151 (MQ=255)
                                                                                                                                           ccccTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg    >  1:216577/1‑151 (MQ=255)
                                                                                                                                            cccTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg    >  2:625735/1‑150 (MQ=255)
                                                                                                                                              cTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGaa  <  1:68956/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA  >  NC_000913/4255359‑4255650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: