Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,504	G→A	H321H (CAC→CAT)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,504	0	G	A	100.0%	190.9 / NA	49	H321H (CAC→CAT)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/17);  total (32/17)

GATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA  >  NC_000913/4255359‑4255650                                                                                                                                                  |                                                                                                                                                   gaTACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTgatgat                                                                                                                                                >  2:349152/1‑150 (MQ=255)    aCTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGCTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATAc                                                                                                                                              >  1:518015/1‑149 (MQ=255)     cTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGc                                                                                                                                           >  1:126137/1‑151 (MQ=255)      tCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGc                                                                                                                                           >  2:687634/1‑150 (MQ=255)              aaCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGa                                                                                                                                   >  2:508670/1‑150 (MQ=255)              aaCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGa                                                                                                                                   >  1:633138/1‑150 (MQ=255)               aCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGaa                                                                                                                                  >  2:515319/1‑150 (MQ=255)                cGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGa                                                                                                                                   >  1:629377/1‑148 (MQ=255)                 ggTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAagc                                                                                                                               <  2:475587/151‑1 (MQ=255)                    ggAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAagcagc                                                                                                                            >  2:598138/1‑151 (MQ=255)                          aaGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAg                                                                                                                      <  2:458287/151‑1 (MQ=255)                              ttATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTcc                                                                                                                   >  2:568759/1‑150 (MQ=255)                                aTTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtg                                                                                                                <  1:560319/151‑1 (MQ=255)                                 ttGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtgt                                                                                                               >  2:3793/1‑151 (MQ=255)                                   gCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtgtg                                                                                                              <  2:590656/150‑1 (MQ=255)                                       ttAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACt                                                                                                           <  2:325118/149‑1 (MQ=255)                                        tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCg                                                                                                        >  2:537132/1‑151 (MQ=255)                                        tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCg                                                                                                        >  1:322201/1‑151 (MQ=255)                                         aaaCGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCgg                                                                                                       <  2:209911/151‑1 (MQ=255)                                             gTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGc                                                                                                   <  1:388488/151‑1 (MQ=255)                                             gTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGc                                                                                                   <  2:369364/151‑1 (MQ=255)                                                cGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCa                                                                                                  >  1:283903/1‑149 (MQ=255)                                                  gCGAATAAAGAACACCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGAACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAgg                                                                                               >  1:42874/1‑150 (MQ=255)                                                       tAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACat                                                                                          >  1:624631/1‑150 (MQ=255)                                                          aGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATAc                                                                                      >  1:174858/1‑151 (MQ=255)                                                            aaCGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATAcc                                                                                     >  2:219557/1‑150 (MQ=255)                                                              cGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGc                                                                                  >  2:249318/1‑151 (MQ=255)                                                                      gACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGAcc                                                                          <  2:610681/151‑1 (MQ=255)                                                                               aaaTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGc                                                                 >  2:615844/1‑151 (MQ=255)                                                                                    ggCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCt                                                            <  1:263377/151‑1 (MQ=255)                                                                                      cccGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTgg                                                          >  1:173063/1‑151 (MQ=255)                                                                                           cAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAAc                                                     <  2:433955/151‑1 (MQ=255)                                                                                               ccAGAAATTCAGGTTGATCCCGGCGGCGATAGGCGGCGGCACCAGCTCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCATCTGGCGAACgcgc                                                 <  1:125940/151‑1 (MQ=255)                                                                                                     aTTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGCGGGCCAGCTGGCGAACGCGCTCAGCg                                           <  1:602598/151‑1 (MQ=255)                                                                                                            ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCACTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGCGGGCCAGCTGGCGAACGCGCTCAGCGTTATgg                                     >  1:536761/1‑150 (MQ=255)                                                                                                               aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGt                                 >  2:207664/1‑151 (MQ=255)                                                                                                                    ggcggcGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATg                            >  2:511554/1‑151 (MQ=255)                                                                                                                    ggcggcGATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATg                            >  1:346768/1‑151 (MQ=255)                                                                                                                       ggcgATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGccc                         >  2:239737/1‑151 (MQ=255)                                                                                                                        gcgATATGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCt                        >  2:267728/1‑151 (MQ=255)                                                                                                                           atatGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGt                     >  2:453045/1‑151 (MQ=255)                                                                                                                             atGCGGCGGCACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTa                    <  2:498301/150‑1 (MQ=255)                                                                                                                                     gcACCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGtt           >  2:51804/1‑151 (MQ=255)                                                                                                                                       aCCAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc         <  1:619928/151‑1 (MQ=255)                                                                                                                                        ccAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc         <  1:393081/150‑1 (MQ=255)                                                                                                                                         cAGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc         >  1:343135/1‑149 (MQ=255)                                                                                                                                          aGCCCCTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAgg      >  2:574155/1‑151 (MQ=255)                                                                                                                                            ccccTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg    >  1:216577/1‑151 (MQ=255)                                                                                                                                             cccTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg    >  2:625735/1‑150 (MQ=255)                                                                                                                                               cTGATGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGaa  <  1:68956/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA  >  NC_000913/4255359‑4255650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑