Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,664,363 (G)6→7 coding (256/729 nt) gadW ← transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,3571.G100.0% 382.0 / NA 98M88L (ATG→CTG) gadWtranscriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (45/53);  total (45/53)

Only 100 of 105 total aligned reads displayed.

AAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCG  >  NC_000913/3664207‑3664502
                                                                                                                                                       |                                                                                                                                                  
aaaCAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                                     >  2:1121946/1‑151 (MQ=255)
  aCAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gg                                                                                                                                                 <  1:137663/151‑1 (MQ=255)
   cAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑ggg                                                                                                                                                >  2:660950/1‑151 (MQ=255)
    aGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg                                                                                                                                               >  4:152531/1‑151 (MQ=255)
       gCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑ggggggg                                                                                                                                            <  1:70937/151‑2 (MQ=255)
           aTGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggggggtgac                                                                                                                                        >  4:439249/1‑146 (MQ=255)
             gCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACt                                                                                                                                        <  2:37640/150‑1 (MQ=255)
                 ttGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGtt                                                                                                                                    <  2:674868/150‑1 (MQ=255)
                  tGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGtta                                                                                                                                   <  4:278552/150‑1 (MQ=255)
                    aaaaGTAATGGGATCAGTTCTTTCTTATGAGAAAACATCTATAAACAGGAAAGGTAGAGTATATTGCTCAGTATCGGCGAATTTACCCCGGATTCATTGAAGCTATCAAAATTCGCCGTCCCCAGCGGCATG‑GGGGGGTGACTgt                                                                                                                                      <  3:148463/145‑2 (MQ=255)
                     aaaGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGttaatta                                                                                                                               <  3:138684/151‑1 (MQ=255)
                      aaGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGttaatta                                                                                                                               >  3:76658/1‑150 (MQ=255)
                       aGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAAc                                                                                                                             >  1:179296/1‑151 (MQ=255)
                       aGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAAc                                                                                                                             >  4:386869/1‑151 (MQ=255)
                          aaGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACaaa                                                                                                                          <  4:394920/151‑1 (MQ=255)
                           aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAAc                                                                                                                         >  4:303484/1‑151 (MQ=255)
                               gATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTc                                                                                                                     >  2:363703/1‑151 (MQ=255)
                                aTCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCt                                                                                                                    <  1:1266569/151‑1 (MQ=255)
                                       ctttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgt                                                                                                             >  1:175316/1‑151 (MQ=255)
                                       ctttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtg                                                                                                              >  2:747189/1‑150 (MQ=255)
                                          tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTgtgt                                                                                                           >  2:778632/1‑150 (MQ=255)
                                           cttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTa                                                                                                          >  4:421580/1‑150 (MQ=255)
                                             tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAcc                                                                                                       >  1:584123/1‑151 (MQ=255)
                                              aTGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAccc                                                                                                      <  3:305370/151‑1 (MQ=255)
                                                gagaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAccc                                                                                                      <  2:602860/150‑1 (MQ=255)
                                                gagaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCt                                                                                                     >  2:48495/1‑150 (MQ=255)
                                                gagaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCt                                                                                                     >  4:158137/1‑150 (MQ=255)
                                                   aaaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAAt                                                                                                 >  3:173520/1‑151 (MQ=255)
                                                     aaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTg                                                                                               >  2:458152/1‑151 (MQ=255)
                                                       cATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCg                                                                                             >  2:323906/1‑151 (MQ=255)
                                                         tCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTg                                                                                           >  2:32602/1‑151 (MQ=255)
                                                           gATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTg                                                                                           >  1:352710/1‑149 (MQ=255)
                                                              aaaCAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                                       <  1:437290/150‑1 (MQ=255)
                                                              aaaCAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGc                                                                                      <  3:151871/151‑1 (MQ=255)
                                                               aaCAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                                                     <  4:155239/151‑1 (MQ=255)
                                                                aCAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                                    <  2:786988/151‑1 (MQ=255)
                                                                  aGGCAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGATATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                                                  >  2:711226/1‑151 (MQ=255)
                                                                   ggAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACg                                                                                 <  1:89168/151‑1 (MQ=255)
                                                                    gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                                <  4:265842/151‑1 (MQ=255)
                                                                     aaaGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                                >  1:555376/1‑150 (MQ=255)
                                                                       aGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                              >  2:1202305/1‑150 (MQ=255)
                                                                        ggTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAatat                                                                            >  1:814642/1‑151 (MQ=255)
                                                                         gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATAtt                                                                           <  1:97221/151‑1 (MQ=255)
                                                                           agagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATAtttt                                                                         >  1:1158488/1‑151 (MQ=255)
                                                                           agagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATAttt                                                                          <  3:113587/150‑1 (MQ=255)
                                                                              gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgta                                                                      <  1:247131/151‑1 (MQ=255)
                                                                              gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgta                                                                      <  2:1243720/151‑1 (MQ=255)
                                                                              gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgt                                                                       >  1:1275340/1‑150 (MQ=255)
                                                                                  atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagta                                                                  <  1:1260405/151‑1 (MQ=255)
                                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagta                                                                  >  2:1209300/1‑150 (MQ=255)
                                                                                    tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                                                >  2:63798/1‑151 (MQ=255)
                                                                                     gCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATg                                                               >  2:1028329/1‑151 (MQ=255)
                                                                                      cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAGCGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATgg                                                              >  4:186434/1‑151 (MQ=255)
                                                                                        cAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATgg                                                              <  1:771262/149‑1 (MQ=255)
                                                                                        cAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                             >  1:511519/1‑150 (MQ=255)
                                                                                        cAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                                            <  4:172594/151‑1 (MQ=255)
                                                                                         aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                                           <  2:287569/151‑1 (MQ=255)
                                                                                         aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                                            >  3:352183/1‑150 (MQ=255)
                                                                                          gTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                                           <  2:802356/150‑1 (MQ=255)
                                                                                            aTCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACt                                                        >  2:403840/1‑151 (MQ=255)
                                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTGACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                      <  1:491030/151‑1 (MQ=255)
                                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTg                                                       <  2:476482/150‑1 (MQ=255)
                                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                      <  3:112757/151‑1 (MQ=255)
                                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                      >  2:283322/1‑151 (MQ=255)
                                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                      <  1:48501/151‑1 (MQ=255)
                                                                                               ggTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTc                                                     <  2:1118785/151‑1 (MQ=255)
                                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTc                                                     >  1:1239004/1‑150 (MQ=255)
                                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCa                                                    <  2:450138/151‑1 (MQ=255)
                                                                                                 tGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTc                                                     >  1:214767/1‑149 (MQ=255)
                                                                                                   aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                                 >  1:556312/1‑151 (MQ=255)
                                                                                                   aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                                 >  1:603646/1‑151 (MQ=255)
                                                                                                    aTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTc                                                >  2:1011635/1‑151 (MQ=255)
                                                                                                       tACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGGTCAt                                              <  2:3682/150‑1 (MQ=255)
                                                                                                       tACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCAt                                              <  2:887406/150‑1 (MQ=255)
                                                                                                        aCCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATca                                            <  2:195489/151‑2 (MQ=255)
                                                                                                        aCCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATc                                             <  2:1019150/150‑1 (MQ=255)
                                                                                                        aCCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCg                                            <  1:1066220/151‑1 (MQ=255)
                                                                                                          cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGat                                          <  1:787852/151‑1 (MQ=255)
                                                                                                          cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGat                                          <  1:84685/151‑1 (MQ=255)
                                                                                                           ccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGat                                          >  1:454164/1‑150 (MQ=255)
                                                                                                              gATTCAATGAAAGTATTAAAAAACGCCGGCCCCCGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATc                                       <  1:1122152/150‑1 (MQ=255)
                                                                                                               aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATcc                                      >  1:188460/1‑150 (MQ=255)
                                                                                                                  cATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                                   <  1:429416/150‑1 (MQ=255)
                                                                                                                  cATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAAGTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtc                                  <  3:317891/151‑1 (MQ=255)
                                                                                                                      gAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgtc                               <  1:737954/150‑1 (MQ=255)
                                                                                                                      gAAGCTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGtcgtcg                              >  2:1281980/1‑151 (MQ=255)
                                                                                                                          cTATCAAAAATCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGt                           >  2:132943/1‑150 (MQ=255)
                                                                                                                                aaaaTCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGt                    >  1:89073/1‑151 (MQ=255)
                                                                                                                                aaaaTCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGt                    >  1:1186026/1‑151 (MQ=255)
                                                                                                                                 aaaTCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTg                   <  3:436993/151‑1 (MQ=255)
                                                                                                                                  aaTCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGa                  <  4:391179/151‑1 (MQ=255)
                                                                                                                                   aTCGCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGt                    >  1:811434/1‑148 (MQ=255)
                                                                                                                                      gCCGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACa               >  2:212371/1‑150 (MQ=255)
                                                                                                                                        cGTCACCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACaaaa            <  2:595792/151‑1 (MQ=255)
                                                                                                                                            aCCAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCa        <  1:1063559/151‑1 (MQ=255)
                                                                                                                                             ccAGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCaa       <  2:1175753/151‑1 (MQ=255)
                                                                                                                                               aGCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCaaaa     <  3:54791/151‑1 (MQ=255)
                                                                                                                                                gCGGCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAAt    <  2:334279/151‑1 (MQ=255)
                                                                                                                                                  ggCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCg  >  1:104537/1‑151 (MQ=255)
                                                                                                                                                  ggCATG‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCg  <  4:38513/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
AAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCG  >  NC_000913/3664207‑3664502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑