Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,031,161 (A)6→7 coding (1/1332 nt) pepQ → proline dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,031,1551.A98.2% 459.2 / ‑1.1 114intergenic (‑184/‑6)fadB/pepQfused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (60/52);  total (62/52)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATC  >  NC_000913/4031005‑4031299
                                                                                                                                                        |                                                                                                                                                 
gTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGt                                                                                                                                                    <  1:442826/151‑1 (MQ=255)
 tACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGt                                                                                                                                                    >  1:97654/1‑150 (MQ=255)
 tACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGt                                                                                                                                                    >  2:479855/1‑150 (MQ=255)
  aCCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaa                                                                                                                                                 <  1:1025522/151‑1 (MQ=255)
  aCCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTa                                                                                                                                                  >  1:1009297/1‑150 (MQ=255)
    cAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaa                                                                                                                                              <  2:992064/151‑1 (MQ=255)
     aGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaa                                                                                                                                             <  1:984206/151‑1 (MQ=255)
     aGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaa                                                                                                                                              >  1:1093247/1‑150 (MQ=255)
     aGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaa                                                                                                                                              >  2:742316/1‑150 (MQ=255)
       aTGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaaa                                                                                                                                            >  1:222171/1‑150 (MQ=255)
         gAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaaaatg                                                                                                                                         <  3:435201/151‑4 (MQ=255)
         gAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaaaatg                                                                                                                                         <  1:634182/151‑4 (MQ=255)
          aGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaaaatgg                                                                                                                                        >  3:343988/1‑147 (MQ=255)
          aGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaaaatgg                                                                                                                                        <  1:901896/151‑5 (MQ=255)
           gTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTaaaaaaatgga                                                                                                                                       <  1:684398/151‑6 (MQ=255)
             cGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAAt                                                                                                                                      <  4:409559/151‑1 (MQ=255)
              gAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATc                                                                                                                                     >  2:1038371/1‑151 (MQ=255)
               aaGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATc                                                                                                                                     >  3:432290/1‑150 (MQ=255)
               aaGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCa                                                                                                                                    <  1:582654/151‑1 (MQ=255)
                    tgtATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGc                                                                                                                               >  2:249185/1‑151 (MQ=255)
                         tttGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGc                                                                                                                          >  4:434229/1‑151 (MQ=255)
                          ttGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGc                                                                                                                          <  2:144344/150‑1 (MQ=255)
                          ttGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGc                                                                                                                          <  2:1235370/150‑1 (MQ=255)
                            gtgtTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGctct                                                                                                                       >  1:1251510/1‑151 (MQ=255)
                            gtgtTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGctct                                                                                                                       <  2:1280244/151‑1 (MQ=255)
                                   aaaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATaaaa                                                                                                                 >  2:1238020/1‑150 (MQ=255)
                                    aaTATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAAt                                                                                                               >  2:854241/1‑151 (MQ=255)
                                     atatGCAAACAAAAGATTAAAGAGATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATc                                                                                                              >  1:677174/1‑151 (MQ=255)
                                          caaacaaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCatat                                                                                                          >  3:445893/1‑150 (MQ=255)
                                           aaacaaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCatat                                                                                                          >  1:524285/1‑149 (MQ=255)
                                           aaacaaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCatat                                                                                                          >  4:22695/1‑149 (MQ=255)
                                             ccacaaGATTAAAGCAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGCAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGc                                                                                                       >  2:202327/5‑150 (MQ=255)
                                             acaaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCt                                                                                                      <  2:750373/151‑1 (MQ=255)
                                              caaaAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCTCTGGCCTCGCTCTATAAAAATCATATAGCTa                                                                                                     <  1:1178837/151‑1 (MQ=255)
                                                aaaGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTAcc                                                                                                   <  2:971867/151‑1 (MQ=255)
                                                  aGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCtt                                                                                                 >  3:408048/1‑151 (MQ=255)
                                                  aGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCtt                                                                                                 <  2:240009/151‑1 (MQ=255)
                                                   gATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCtt                                                                                                 >  2:578340/1‑150 (MQ=255)
                                                   gATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTa                                                                                                <  1:707757/151‑1 (MQ=255)
                                                       aaaGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAg                                                                                            <  1:1052395/151‑1 (MQ=255)
                                                        aaGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACa                                                                                              >  2:859352/1‑148 (MQ=255)
                                                         aGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGaa                                                                                          <  1:537585/151‑1 (MQ=255)
                                                           aaaTGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACg                                                                                        <  3:373683/151‑1 (MQ=255)
                                                           aaaTGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACg                                                                                        >  2:139762/1‑151 (MQ=255)
                                                            aaTGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACgg                                                                                       >  2:1236309/1‑151 (MQ=255)
                                                            aaTGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACgg                                                                                       >  1:854515/1‑151 (MQ=255)
                                                              tGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGAc                                                                                     >  2:631290/1‑151 (MQ=255)
                                                                   aTCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTcgcg                                                                                >  2:613347/1‑151 (MQ=255)
                                                                     cTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGAt                                                                              >  2:209271/1‑151 (MQ=255)
                                                                       gATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTcgc                                                                                 <  3:295305/146‑1 (MQ=255)
                                                                       gATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTcgc                                                                                 <  2:977034/146‑1 (MQ=255)
                                                                       gATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTcgc                                                                                 <  2:880854/146‑1 (MQ=255)
                                                                       gATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTcg                                                                                  <  1:1101878/145‑1 (MQ=255)
                                                                        aTCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATgcg                                                                           <  2:1202972/151‑1 (MQ=255)
                                                                        aTCACGCGCGGCAGAAATCACGGGCTGGATGGACGATGTGCTAAGATGCGGAGACGTAAGGTCAAAAAAACAGAAGAGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATgc                                                                            >  3:110367/1‑150 (MQ=255)
                                                                         tCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATgcgc                                                                          >  1:431581/1‑151 (MQ=255)
                                                                         tCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATgcgc                                                                          >  3:281900/1‑151 (MQ=255)
                                                                          cACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCt                                                                         <  3:192969/151‑1 (MQ=255)
                                                                           aCGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTg                                                                        >  1:589516/1‑151 (MQ=255)
                                                                            cGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTgg                                                                       <  1:271232/151‑1 (MQ=255)
                                                                               tCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGgcgc                                                                    >  3:181536/1‑151 (MQ=255)
                                                                                cGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGgcgcg                                                                   <  2:568254/151‑1 (MQ=255)
                                                                                    aGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTc                                                               >  3:313589/1‑151 (MQ=255)
                                                                                     gAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTc                                                               >  1:391115/1‑150 (MQ=255)
                                                                                     gAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTc                                                               >  2:338004/1‑150 (MQ=255)
                                                                                     gAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCa                                                              <  1:1199693/151‑1 (MQ=255)
                                                                                      aaaTCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCa                                                              >  2:545211/1‑150 (MQ=255)
                                                                                          cACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCt                                                          <  2:729673/150‑1 (MQ=255)
                                                                                            cGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTgg                                                        >  4:30225/1‑150 (MQ=255)
                                                                                                 tGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGt                                                  >  4:206372/1‑151 (MQ=255)
                                                                                                    aTGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTAc                                               <  2:105125/151‑1 (MQ=255)
                                                                                                     tGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACt                                              >  4:298168/1‑151 (MQ=255)
                                                                                                      gAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACtt                                             <  4:225001/151‑1 (MQ=255)
                                                                                                         cGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTAtt                                          <  1:284806/151‑1 (MQ=255)
                                                                                                         cGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTAtt                                          >  4:106/1‑151 (MQ=255)
                                                                                                          gATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTc                                         >  3:213016/1‑151 (MQ=255)
                                                                                                           aTGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCa                                        <  1:58234/151‑1 (MQ=255)
                                                                                                            tgtgCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCAc                                       >  1:1264603/1‑151 (MQ=255)
                                                                                                            tgtgCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCAc                                       >  1:543423/1‑151 (MQ=255)
                                                                                                             gtgCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCAc                                       <  1:7312/150‑1 (MQ=255)
                                                                                                               gCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTca                                    <  2:1056806/151‑2 (MQ=255)
                                                                                                                  aaGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGc                                 <  2:1009101/151‑1 (MQ=255)
                                                                                                                       gCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGc                             <  1:1238244/150‑1 (MQ=255)
                                                                                                                        cGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTg                           >  3:347293/1‑151 (MQ=255)
                                                                                                                            gaCTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCa                       <  3:87848/151‑1 (MQ=255)
                                                                                                                             aCTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCa                       >  1:1172245/1‑150 (MQ=255)
                                                                                                                              cTTAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAAt                     >  3:338698/1‑151 (MQ=255)
                                                                                                                                tAAGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAAt                     <  4:286412/149‑1 (MQ=255)
                                                                                                                                 aaGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGtt                  >  2:1154580/1‑151 (MQ=255)
                                                                                                                                 aaGGTCAAAAAAACAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGtt                  <  2:421631/151‑1 (MQ=255)
                                                                                                                                       aaaaaaaCAGAAGGGTAAAAAAATTGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTtctc            >  4:124232/1‑151 (MQ=255)
                                                                                                                                       aaaaaaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTtctc            <  2:96890/151‑1 (MQ=255)
                                                                                                                                       aaaaaaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTtct             <  2:958830/150‑1 (MQ=255)
                                                                                                                                          aaaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTcgac         <  1:732422/151‑1 (MQ=255)
                                                                                                                                          aaaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTcgac         <  1:878836/151‑1 (MQ=255)
                                                                                                                                           aaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTcgacg        <  3:38192/151‑1 (MQ=255)
                                                                                                                                           aaaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTcgacg        >  2:467136/1‑151 (MQ=255)
                                                                                                                                            aaCAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTcgacg        >  1:1078255/1‑150 (MQ=255)
                                                                                                                                              cAGAAGGGTAAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGatc     <  2:524187/151‑1 (MQ=255)
                                                                                                                                                 atgggTAACAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGatcatc  >  2:1207938/3‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                 
GTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGGTAAAAAATGGAATCACTGGCCTCGCTCTATAAAAATCATATAGCTACCTTACAAGAACGGACTCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATC  >  NC_000913/4031005‑4031299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: