Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG41.3% 121.5 / 11.4 75G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (9/35);  new base G (31/0);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 2.52e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCG  >  NC_000913/213192‑213486
                                                                                                                                                      |                                                                                                                                                   
gATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGaa                                                                                                                                                    <  3:23006/151‑1 (MQ=255)
gATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGaa                                                                                                                                                    <  1:745598/151‑1 (MQ=255)
  tGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAg                                                                                                                                                   >  1:451189/1‑150 (MQ=255)
   gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgt                                                                                                                                                  <  1:1096495/150‑1 (MQ=255)
     cTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAgggt                                                                                                                                                >  1:851496/1‑150 (MQ=255)
       cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGTGGCTGGGAAGTGTa                                                                                                                                               <  4:180418/149‑1 (MQ=255)
       cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACa                                                                                                                                            <  4:339187/151‑1 (MQ=255)
         cccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAg                                                                                                                                           <  3:442125/150‑1 (MQ=255)
          ccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAg                                                                                                                                           >  1:58035/1‑149 (MQ=255)
            tGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGCt                                                                                                                                         >  1:1236821/1‑149 (MQ=255)
             ttttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCtt                                                                                                                                        >  1:1089219/2‑149 (MQ=255)
             gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCtt                                                                                                                                        >  1:548467/1‑149 (MQ=255)
              tttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCCTaa                                                                                                                                      >  1:813406/1‑150 (MQ=255)
               ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAAt                                                                                                                                     >  4:406539/1‑150 (MQ=255)
               ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAt                                                                                                                                     <  1:114347/150‑1 (MQ=255)
                tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAggggaacggctta                                                                                                                                       >  3:438358/1‑136 (MQ=255)
                tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTAAAGCTTaa                                                                                                                                      >  1:51898/1‑148 (MQ=255)
                tACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGTCAGCTTaa                                                                                                                                      >  2:1045946/1‑148 (MQ=255)
                  cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATGCgg                                                                                                                                 >  1:438161/1‑151 (MQ=255)
                      tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAg                                                                                                                              <  1:1028367/150‑1 (MQ=255)
                       gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAgg                                                                                                                             <  4:106284/150‑1 (MQ=255)
                         gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAAGCGGGAGGCg                                                                                                                           >  1:559609/1‑150 (MQ=255)
                         gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAgggg                                                                                                                           >  1:880040/1‑148 (MQ=255)
                           gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGAGGCGat                                                                                                                         >  4:364088/1‑150 (MQ=255)
                            cgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGAGGAAAGCTTAATGCGGGGGGCGata                                                                                                                        >  3:156183/1‑150 (MQ=255)
                                 gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGcc                                                                                                                  <  2:198158/151‑1 (MQ=255)
                                      ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATGCGGGGGGGGGTATTTGCCCTc                                                                                                               >  4:428908/1‑149 (MQ=255)
                                      ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTcca                                                                                                             <  2:499286/151‑2 (MQ=255)
                                          cGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGCTTAATGAGGGGGGCGAGATTCACCCTCcgcgcg                                                                                                         >  1:1164660/1‑149 (MQ=255)
                                             tATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTAAAGCTTAATGCGGGGGGCGATATTCGCCCCCCCCGTGccg                                                                                                      >  4:381248/1‑149 (MQ=255)
                                              aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGGGGGGGTATTCGCCCTCcgc                                                                                                            >  4:287688/1‑144 (MQ=255)
                                                cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGAc                                                                                                    <  1:17141/150‑1 (MQ=255)
                                                    cGCACCTGTGGTGCATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCCGCCGCTGGAATTGCCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGaa                                                                                                 <  2:406120/149‑1 (MQ=255)
                                                    cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGc                                                                                               <  1:971539/151‑1 (MQ=255)
                                                     gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCg                                                                                              <  1:1261989/151‑1 (MQ=255)
                                                        aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGGGGACAACCTTAATCGGGGGGGGGAACTCGCCCTCCCCCGGCAGAAGAAGCg                                                                                              >  4:448315/1‑148 (MQ=255)
                                                         cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAACTTTATGCGGGGGGGGGAATTTTCCCTTCCCCGGCAGAAGAAGCgg                                                                                             >  1:966199/1‑148 (MQ=255)
                                                         cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGCGGGAGGGGATATTTGCCCCCCGCGGGCAGACGGAGCgggt                                                                                           >  1:740091/1‑148 (MQ=255)
                                                          tgtgGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTTATGCGGGAGGGGGTATTCGCCCTCCGCGTGCAGACGAAGCgg                                                                                             >  4:373807/1‑147 (MQ=255)
                                                            tggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCGATATTCGCCCTCCGCGTGCAGAAGAAGAGGGCAgcg                                                                                       >  3:433143/1‑151 (MQ=255)
                                                                  aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTTAAACTTAATGCGGGGGGGGGTATTATCCCCCCCCGGGCAGAAGAAACGGGCAgcggggg                                                                                   >  2:1136678/1‑145 (MQ=255)
                                                                    tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGGGGGGATATTTGCCCTCCGCGGGCAGACGAAACGGTCAGCGCGCGgtt                                                                               >  1:77032/1‑150 (MQ=255)
                                                                     aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGGAAAGCTTAATGCGGGAGGCGATATTTGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGttt                                                                               >  1:911544/1‑149 (MQ=255)
                                                                     aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCa                                                                             <  2:559504/151‑1 (MQ=255)
                                                                      aaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGAGGGAAGCTTTATGCGGGGGGGGATATTCGCCCCCCGCGCGCAGACGAAGCGGTCAGCGGGCGgttta                                                                             >  3:159413/1‑148 (MQ=255)
                                                                       aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCaaa                                                                           <  2:402471/151‑1 (MQ=255)
                                                                         ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc                                                                         <  1:566592/151‑1 (MQ=255)
                                                                            tCACGGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGGGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAgc                                                                         <  2:1068483/148‑1 (MQ=255)
                                                                             cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGGGCGTTTCAAAACGcc                                                                      >  4:45203/1‑150 (MQ=255)
                                                                              aCCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAg                                                                    <  4:285569/151‑1 (MQ=255)
                                                                               ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAg                                                                    <  2:664406/150‑1 (MQ=255)
                                                                               ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAg                                                                    <  4:204999/150‑1 (MQ=255)
                                                                                cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGa                                                                  <  1:1272191/151‑1 (MQ=255)
                                                                                 gggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGAt                                                                 <  1:724838/151‑1 (MQ=255)
                                                                                  ggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGATATTTGCCCTCCGCGTGCCGACGAAGCGGGCAGCGGGCGGTTCTAAGCGCCCGGAtt                                                                >  4:290224/1‑151 (MQ=255)
                                                                                     aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgct                                                             <  2:114031/151‑1 (MQ=255)
                                                                                      aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgctg                                                            <  4:51510/151‑1 (MQ=255)
                                                                                           aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTCGGGGGGGGGTATTTGCCCTCCCCGGGCAGACGAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGGTTGCTGCat                                                         >  4:415015/1‑149 (MQ=255)
                                                                                           aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCatat                                                       <  2:501918/151‑1 (MQ=255)
                                                                                            aaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATAtt                                                      <  1:398378/151‑1 (MQ=255)
                                                                                             aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGGGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCCCGTGCAGACGAAGCGGTCAGCGGGCGTTTCAAAGCGCCAGGATTGCTGCATAtt                                                      >  4:27909/1‑150 (MQ=255)
                                                                                               cATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTTATGCGGGGGGGGACATTCTCCCCCCGCGTGCAGACGGAGCGGTTAGCGGGCGGTTTCAAAAGCCCGGATTGCTGGat                                                         >  2:1046431/1‑145 (MQ=255)
                                                                                                  tgtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCgg                                                 <  1:1274913/150‑1 (MQ=255)
                                                                                                     gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTGGAGCTTAAATGGGGGGGGGGTATTCCCCCTCCGCGGGCAGACGAAGAGGTCCGCGTGCGTGTTCAAGCGCCCAGGGTGGTGGATATTGTCgggggg                                             >  2:138317/1‑148 (MQ=255)
                                                                                                      ccGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTa                                            <  1:71368/151‑1 (MQ=255)
                                                                                                       cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCCGGGAAAGCAAAACACTAATCGGGAGGCGATAATCGCCCTCCGCGGGCAGAGGAAGAGGGCCACCTGCGGTTTCAACCGCCCAGGATGCTCGATATTGTcgcgcgg                                             >  1:1275112/1‑148 (MQ=255)
                                                                                                           gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                       <  1:1246021/151‑1 (MQ=255)
                                                                                                             aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggcgg                                     <  3:408015/151‑1 (MQ=255)
                                                                                                                         aaCCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAATGCGGGAGGCGATATTTGCCCTCCGCGGGCAGACGAAGCGGGCCGCGTGCGGTTCAAAACGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGGCGTAAGGTaa                          >  1:160890/1‑150 (MQ=255)
                                                                                                                           ccGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGa                       <  2:702877/151‑1 (MQ=255)
                                                                                                                                ggAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAAt                   <  1:183743/150‑1 (MQ=255)
                                                                                                                                         ggcggGGCTGGGAAGTGGACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGGGTTTCAAAGCGCCAGGGTTGCTGCATATTGTCGGGCGTAACGGCGGACGGAAGCTTAAGAAAAttcggcca            >  2:1215276/1‑141 (MQ=255)
                                                                                                                                          gcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc        >  1:261836/1‑151 (MQ=255)
                                                                                                                                            ggggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTg      >  2:641822/1‑151 (MQ=255)
                                                                                                                                            ggggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTg      <  2:1236597/151‑1 (MQ=255)
                                                                                                                                              ggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTggg    <  4:338762/151‑1 (MQ=255)
                                                                                                                                                   gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg  <  1:490154/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCG  >  NC_000913/213192‑213486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: