Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA43.7% 77.2 / 43.1 71intergenic (‑124/+79)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (34/6);  new base A (0/31);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 3.19e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAT  >  NC_000913/1824911‑1825210
                                                                                                                                                          |                                                                                                                                                     
cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                                       <  2:721120/151‑1 (MQ=255)
cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                                       <  1:135975/151‑1 (MQ=255)
cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                                       <  4:6175/151‑1 (MQ=255)
    ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                                    <  4:310259/150‑1 (MQ=255)
      aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                                 <  1:913888/151‑1 (MQ=255)
       cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                                <  1:320555/151‑1 (MQ=255)
       cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTCTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                                >  4:61655/1‑151 (MQ=255)
             tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                                          >  1:599554/1‑151 (MQ=255)
             acAAAGTATTCCATACTCGCTCCCGTCCCCTTCTTTTCATTGTTATCGTTCATATAACCGAGGATGGCTATCGGCATCTCGGGTGCTTCACGTTAGTAGAGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGATCACCGCCGAt                                                                                                                                           >  2:419771/2‑150 (MQ=255)
               aaattttcccATACTCGCTCCCGGCCCTTTCTTTTCATTGTTATCGTTCAGGTAACCGGGGATGTTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATc                                                                                                                                          <  2:943977/141‑1 (MQ=255)
               aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAAAAAAGAAAAccgccg                                                                                                                                             <  4:83992/146‑1 (MQ=255)
                     ttCCATCCTCCCCCCCGGCCCGTTTTTTTCTTTTTTATCGTTCAGGTACCCGGGGATGGTTATCGGCAACTGGGGTGATTTAGGTTAGTGGTGATCAGGTTTTTTTAACAATTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                                                   <  1:819608/150‑1 (MQ=255)
                     ttCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGCTGGTTATCGGCAACTCGGGGGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGCTCCTGTCCAc                                                                                                                                  >  2:634284/1‑151 (MQ=255)
                      tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                                 >  1:107010/1‑151 (MQ=255)
                       ccATCCCCGCCCCCGGCCCTTTTTTTTCATTTTTATCGTTAAGGTACCGGTGGATGTTTATCGGCAACGGGGGTGTTTTCCGTTAGTGTTGATCAGTTTTTTTTTACAACTTTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                                                   <  1:953290/148‑1 (MQ=255)
                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                          >  1:747889/1‑151 (MQ=255)
                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                          >  1:522103/1‑151 (MQ=255)
                              cGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                         >  1:1058996/1‑151 (MQ=255)
                                  cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                      >  1:127631/1‑150 (MQ=255)
                                   ccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                      >  3:235204/1‑149 (MQ=255)
                                    tggCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                                   >  2:422395/2‑151 (MQ=255)
                                         cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                              >  4:11919/1‑151 (MQ=255)
                                          gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                              >  2:41359/1‑150 (MQ=255)
                                             gttttCATTGTTATCGTTCAGGTAACCGTGGCTGGTTATCGGCAACTCGGGGGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCAACCGCATTACTGCAAGGTAGTGGAc                                                                                                           >  2:112735/2‑150 (MQ=255)
                                              ttttCTTTGTTATCGTTCAGGTACCCGTGGATGTTTGTGGGCAACGGGTTTTTTTTACGTTAGGGGTCACCGGGTTTTTTTAAAAGCTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                          <  2:323590/150‑1 (MQ=255)
                                              ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                          >  2:1079206/1‑150 (MQ=255)
                                                    ttGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                                   >  1:1199943/1‑151 (MQ=255)
                                                      tgtttccttCAGGTTAACGGGGGTTGGTTATCGACAACTGGGGTTTTTTAGGTTAGTGGTCACCGGTTTTTTTTAAAAGCTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                                   <  2:189926/143‑1 (MQ=255)
                                                       ttttCGTGCAGGTAACGGGGGAGGGTTATCGGCACCCGGGGTAATTTCCGTTGGTGGTAATCGGTTTTTTTTCACATTTTTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                                 <  1:766168/147‑1 (MQ=255)
                                                       ttATGGTTCAGGTAACGGGAGATGGTTATCGACACGGGGGTTTATTTTCGTGAGTGTTGACGGGGTTTTTTTCACTACTGTTAGAAAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGa                                                                                                       <  2:503518/144‑1 (MQ=255)
                                                       ttATCGTTCAGTTACCCGTGGTTGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                                 <  4:134270/150‑1 (MQ=255)
                                                        tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                                >  3:366835/1‑150 (MQ=255)
                                                           ttgttaagttatctgTGGATGTTTTCGGGAATCTGGGGTGTTTTTCGTGAGTGGTAATTAGGTTTTTTTTACAAGTGTTAGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                              <  1:507611/136‑1 (MQ=255)
                                                           cGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                            >  1:308142/1‑151 (MQ=255)
                                                                  gTACCCGTGGTTGTTTTTCGACATCTGGGTTGTTTTGCTTTGGTGGTATCCGGTTTTTTTTTACATCTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttt                                                                                      <  2:1200046/150‑1 (MQ=255)
                                                                      aacGTGGATGTTTATGCGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAACACCGCCGAACCTGTCCAACGCATTACTGTAAGGTAGTGGACAAGAACGGCGGTCTTTAGcttt                                                                                     >  3:375975/3‑148 (MQ=255)
                                                                               ggTTATCGGCACCTGGGGTGATTTCCGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                            <  1:782193/148‑1 (MQ=255)
                                                                               ggTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTgga                                                                            >  2:657224/1‑146 (MQ=255)
                                                                               ggTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                            >  1:397168/1‑148 (MQ=255)
                                                                                      ggCAACTCGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                                  <  3:109684/151‑1 (MQ=255)
                                                                                      gacAACTGGGGTTTTTTGCGTAGGGGGTGATCGGTTTTTTTTTCCAGTTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattat                                                                     <  1:1140858/146‑1 (MQ=255)
                                                                                          acctggggTGTTTTACGTTAGTGTTGATCGGGTTTTTTTTAAAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                               <  1:734049/146‑1 (MQ=255)
                                                                                          acctggggTGATTTACGTTGGTGGTGATCAGTTTTTTTTAAAAACTGTTGAAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                                <  1:103104/145‑1 (MQ=255)
                                                                                               gggTTATTTACGTTAGTGGTGCTCAGGCTTTTTTTACATCTGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                                <  2:851958/145‑1 (MQ=255)
                                                                                               gggTGTTTTCCGTTAGTGGTGATCGGGTTTTTTTTACAATTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagcag                                                              <  1:704800/147‑1 (MQ=255)
                                                                                               gggTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                            >  4:425601/1‑149 (MQ=255)
                                                                                               gggTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                           >  1:1042031/1‑150 (MQ=255)
                                                                                                 gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                          >  2:59206/1‑149 (MQ=255)
                                                                                                     tttACGTTAGTGGTGATCGGTTTTTTTTTACAGCTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGc                                                      <  1:770279/149‑1 (MQ=255)
                                                                                                     tttACGTGAGGGGTATCCAGGTTTTTTTTACAACTGTTGGAAAAAAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGc                                                      <  3:341583/149‑1 (MQ=255)
                                                                                                      taaCGTTGGTGTTGATCAGGTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagcag                                                              <  2:132512/138‑1 (MQ=255)
                                                                                                        aCGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                                 >  3:267083/1‑151 (MQ=255)
                                                                                                        aCGTTAGGGGTGATCAGGTTTTTTTTACAATTTTTGAAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                                 <  1:513925/151‑1 (MQ=255)
                                                                                                        aCGTGAGTGGTGAACAGGTTTTTTTTACAACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                                  <  1:385204/150‑1 (MQ=255)
                                                                                                          gTTAGGGGTGTTCGGTTTTTTTTTACAGCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                                 <  1:1255354/149‑1 (MQ=255)
                                                                                                           ttggggttgACCAGGTTTTTTTTACATTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                                <  4:60065/142‑1 (MQ=255)
                                                                                                           ttATTGGTGACCTGTTTTTTTTTACATCTGTTGGAAAAAAAAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                               <  1:659049/150‑1 (MQ=255)
                                                                                                            tAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCCGCAGTTGCAGGCATTTTAcc                                              >  1:548974/1‑150 (MQ=255)
                                                                                                                ggtggtCGGGTTTTTTTTACACCTGTTTGAAAAAGAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                           <  3:342702/149‑1 (MQ=255)
                                                                                                                ggtgATCAGGTTTTTTTTACAATTTTTAGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                         <  1:66172/151‑1 (MQ=255)
                                                                                                                ggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCtttt                                          >  1:995408/1‑150 (MQ=255)
                                                                                                                   tgatcggtttttttttACAACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGc                                        <  2:537209/141‑1 (MQ=255)
                                                                                                                    aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcccc                                     >  1:182137/1‑149 (MQ=255)
                                                                                                                       aGGTTTTTTTTAAAACTTTTTAAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCg                                      <  4:90419/147‑1 (MQ=255)
                                                                                                                           ttttttttACATTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                                    <  2:949907/145‑1 (MQ=255)
                                                                                                                            tttttttACACTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                                  <  1:1095969/147‑1 (MQ=255)
                                                                                                                            ttttttaACAACTGTTTAAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAc                                <  3:34894/149‑1 (MQ=255)
                                                                                                                                tttACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                           <  1:27409/150‑1 (MQ=255)
                                                                                                                                 ttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                           >  4:64345/1‑149 (MQ=255)
                                                                                                                                  tACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTATTAAGTTTTTTGGCTGAAAGAATATTCAGCAGATGCAGGCATTTTATCTTTTGCCGCTGGAAGTTCTGt                         >  1:1274161/1‑150 (MQ=255)
                                                                                                                                    cAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                         >  1:1042939/1‑148 (MQ=255)
                                                                                                                                         tttttGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                         <  1:257474/142‑1 (MQ=255)
                                                                                                                                            ttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt                >  1:380220/1‑150 (MQ=255)
                                                                                                                                             tGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGGAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc               >  2:1066215/1‑150 (MQ=255)
                                                                                                                                                  agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCcaa          >  1:479527/1‑150 (MQ=255)
                                                                                                                                                  aaaaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGGCGTGCTGTCAGACGCTTCTCaaaa         <  2:5474/147‑1 (MQ=255)
                                                                                                                                                    agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt       >  2:569662/1‑151 (MQ=255)
                                                                                                                                                    aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaaa         <  4:71211/147‑1 (MQ=255)
                                                                                                                                                    aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt       <  1:284606/149‑1 (MQ=255)
                                                                                                                                                    aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt       <  2:1103806/149‑1 (MQ=255)
                                                                                                                                                       aaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc    >  1:835046/1‑151 (MQ=255)
                                                                                                                                                       aaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc    <  4:442784/151‑1 (MQ=255)
                                                                                                                                                        aagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc    >  2:923194/1‑150 (MQ=255)
                                                                                                                                                        aagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGATTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt       >  2:833783/1‑147 (MQ=255)
                                                                                                                                                         agaaaaCCGCCGATCCTGGCCACCGCATTCCTGCAAGGTAGTGGACAAGACCGGCGGGCTTTAGTTTTTTGGCTGAAAGATTATTGAGCCGGTGACGGCATTTTACCGTTTGCCGCTGGACGTTCTGTCAAGCTCTTTTCAAAATTAGGAt  >  4:353375/1‑151 (MQ=255)
                                                                                                                                                          |                                                                                                                                                     
CATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAT  >  NC_000913/1824911‑1825210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: