Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA41.2% 153.0 / 61.8 114E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (45/22);  new base A (0/47);  total (45/69)
Fisher's exact test for biased strand distribution p-value = 2.44e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGG  >  NC_000913/3177600‑3177893
                                                                                                                                                     |                                                                                                                                                 
cTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCa                                                                                                                                                  <  1:69017/150‑1 (MQ=255)
  ttCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCaa                                                                                                                                                 <  4:420296/149‑1 (MQ=255)
  ttCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCaa                                                                                                                                                 <  1:430119/149‑1 (MQ=255)
    cACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCaaaaa                                                                                                                                              <  2:765281/150‑1 (MQ=255)
      cccTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAAttt                                                                                                                                           >  4:132909/1‑151 (MQ=255)
        ctctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTcc                                                                                                                                         >  3:308797/1‑151 (MQ=255)
         tctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTccc                                                                                                                                        >  2:1174470/1‑151 (MQ=255)
             cAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                     <  2:711849/150‑1 (MQ=255)
              aataatCCCGGCAACCATCTCGAGTAGCCAAGGGGTTGCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTAAATAAGCACAACTTCATCACGCACTGGGTAAAAGGGTGG‑CACGACTGCGGCGTGACCGCGCTCAAAAATTTc                                                                                                                                          >  3:49343/1‑144 (MQ=255)
                 catcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAcc                                                                                                                                >  1:705019/1‑151 (MQ=255)
                 catcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAc                                                                                                                                 <  2:470721/150‑1 (MQ=255)
                  atcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCt                                                                                                                               >  1:1057420/1‑151 (MQ=255)
                    cccGGCAACCATCTCCAGTAGCCAGGGGGTTTCGCGGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACGGGGTCAAAGGGTAG‑CAAGCCGGGGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCAcca                                                                                                                               <  2:1266379/149‑2 (MQ=255)
                      cGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATg                                                                                                                           >  1:144491/1‑151 (MQ=255)
                      cGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATg                                                                                                                           >  2:696089/1‑151 (MQ=255)
                      cGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATg                                                                                                                           >  1:360432/1‑151 (MQ=255)
                      cGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAACTTCCCGCCGCACCTCATg                                                                                                                           >  2:898205/1‑151 (MQ=255)
                       ggCACCCATCCCCAGTAGCAAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCCCTGGGTCAAGGGGTAG‑CAAGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATg                                                                                                                           <  2:1205057/150‑1 (MQ=255)
                         cAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACt                                                                                                                        >  1:1156561/1‑151 (MQ=255)
                               tctcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                  >  1:1246867/1‑151 (MQ=255)
                                ctcCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGc                                                                                                                 >  3:440447/1‑151 (MQ=255)
                                ccccAGTGGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCAAATCTTCTCAACCAGCACACCTTCATCACGCCCTGGGTCAAGGGGTAG‑CAAGACTGCGGCGTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTg                                                                                                                  <  1:746216/148‑1 (MQ=255)
                                ccccAGTGCCCAAGGGTTTTCGCTGGTGTCGTCCGCGGCAATCCGAATTTGCTCAATCAGCAAAACTTCACCACGCCCTGGGTCAAAGGGTAC‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                  <  2:465781/148‑1 (MQ=255)
                                 tccgggacccAGGGGTTTTCGCTGGTCTCGTACGCGCCAATCCTAATCTGCTCAACCAGCACAACTTCACCACGCACTGGGTCAAAGGTTAG‑CAAGACGCCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGAca                                                                                                                        <  2:649759/135‑2 (MQ=255)
                                  ccAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                  <  4:118165/148‑1 (MQ=255)
                                  ccAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCCCACTGGGTCAAGGGGTAG‑CAAGACGGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                  <  4:242832/148‑1 (MQ=255)
                                  ccAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGccc                                                                                                               >  2:711192/1‑151 (MQ=255)
                                    aGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCATTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCACAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                              <  3:112672/150‑1 (MQ=255)
                                      tAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCCCTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt                                                                                                            <  1:857699/150‑1 (MQ=255)
                                         ccAGGGGTTTTCGTTGGTGCCGTACGC‑GCAAACCCAATCTGCTCAATCAGCACAACTTCATCACGCACTGGTTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGa                                                                                                          <  1:921895/148‑1 (MQ=255)
                                          cAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                        >  4:339880/1‑150 (MQ=255)
                                           aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                        >  1:1238529/1‑149 (MQ=255)
                                           aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                       <  1:1066746/150‑1 (MQ=255)
                                               ggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                   >  1:625490/1‑150 (MQ=255)
                                                gTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                                                                                                 >  3:378038/1‑151 (MQ=255)
                                                 tttCGCTGGTGTCGTACGCGGCAACCCAAATCTGCTCAATCAGCACAACTCCATCACCCACTGGGTAAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                                                                                                 <  3:373014/150‑1 (MQ=255)
                                                  ttCGTTGGTTTCGTACGCGGAAATCCGAATCTGCTCAATCAGCACACCTTCATCACGCACGGGGTCAAGGGTTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                <  3:253703/150‑1 (MQ=255)
                                                       tGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                             <  2:466927/148‑1 (MQ=255)
                                                        ggtgtcggcgccgtaaaTCCAAATCTGCTCAACCACCACACCTTCTCAACGCACTGGGTCAAAGGTTAG‑CAAGGTTGCGGGGTAACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa                                                                                          <  1:1249907/135‑1 (MQ=255)
                                                        ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGTTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa                                                                                          <  1:510126/150‑1 (MQ=255)
                                                        ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                             <  1:15069/147‑1 (MQ=255)
                                                          tgtCGTCCGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGCCTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                        <  2:1021095/150‑1 (MQ=255)
                                                          tgccgaaCGCGGCAATCCGAATCTGCTCAATCAGCACACCTTCATCACCCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                        <  2:54837/144‑1 (MQ=255)
                                                           gtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt                                                                                       >  1:1215742/1‑150 (MQ=255)
                                                            tCGTACGCGGCAATCCGATTCTGCCAAACCAGCACAACTTCACCCCGCACTGGGCAAAAGGGTAG‑CAAGCCTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAt                                                                                         <  2:899394/147‑1 (MQ=255)
                                                              gTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                    >  3:451981/1‑150 (MQ=255)
                                                               tccgcgGCAACCCGAATCTGCTAAATCAGCACAACTTCATCACGCACTGGGCCAAAGGGTAG‑CAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCtata                                                                                    <  1:785596/147‑1 (MQ=255)
                                                                  gcgGCAATCCGAATCTGCTCAATCAACACAACTCCATCCCGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                               <  1:149227/151‑1 (MQ=255)
                                                                  gcgGAAATCCGAATCTCCTCAATCAGCACAACTTCATCCCGCACGGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                               <  1:304485/151‑1 (MQ=255)
                                                                    ggCAATCCAAATCTGCTCAATCAGCACACCTCCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc                                                                              <  4:357898/150‑1 (MQ=255)
                                                                       aacccGATTCTGGTCAATCAGCACACCTTCACCCCGCTCGGGCAAAAAGGGTAG‑AAAGCCTGCGGGGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGa                                                                                <  2:65300/142‑1 (MQ=255)
                                                                       aaTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt                                                                          <  4:34324/151‑1 (MQ=255)
                                                                       aaTCCGAATCTGCTCAATCAGCACAACTTCACCCCGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCCCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAt                                                                          <  1:389597/151‑1 (MQ=255)
                                                                          ccGAATCTGCCCAATCAGCCCAACTCCACCGCGCACGGGTTCAAGGGGTAC‑CAAGCAGGCGGCGTGCCCGCTATAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                       <  2:137484/151‑1 (MQ=255)
                                                                           cGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                       >  2:1150259/1‑150 (MQ=255)
                                                                                 tGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGc                                                                >  2:1276532/1‑151 (MQ=255)
                                                                                  gCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                               >  1:1070487/1‑151 (MQ=255)
                                                                                  gCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑AAAGACTGGGGCGTGCCCGCCCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                               <  1:1174683/151‑1 (MQ=255)
                                                                                   cTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                              >  2:75957/1‑151 (MQ=255)
                                                                                   cTCAATCAGCACAACTTCACCCCGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                               <  4:18273/150‑1 (MQ=255)
                                                                                      aataaccacaACTTCATCCCGCATGGGGACAAAGGGTAG‑AAGGACTGGGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                               <  2:122101/141‑1 (MQ=255)
                                                                                      aaTCACCACAACTTCACCCCGCACGGGGTCAAAGGGAAG‑CAAGACGGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGa                                                           <  2:630234/151‑1 (MQ=255)
                                                                                       aTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGTATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAATCCGCGa                                                           >  2:1132196/1‑150 (MQ=255)
                                                                                       aTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACGGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                          <  3:272775/151‑1 (MQ=255)
                                                                                        tCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                          >  4:377432/1‑150 (MQ=255)
                                                                                          aGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATaa                                                        >  1:261476/1‑150 (MQ=255)
                                                                                             acaACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg                                                    >  2:1109545/1‑151 (MQ=255)
                                                                                             acaACTCCACCCCGCCCGGGGTAAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgt                                                     <  1:422219/150‑1 (MQ=255)
                                                                                              cacctccaccaCGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGa                                                           <  2:653069/134‑1 (MQ=255)
                                                                                               aaCTTCACCCCCCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg                                                    <  2:351209/149‑1 (MQ=255)
                                                                                                  ttCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtct                                               >  2:1023149/1‑151 (MQ=255)
                                                                                                  ttCACCACACACTGGGTAAAATGGCAG‑ACAGTCTGCGGCGTGACCGCACCAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGttt                                                 <  2:1231239/149‑1 (MQ=255)
                                                                                                     atcaCGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGt                                            >  2:1061436/1‑151 (MQ=255)
                                                                                                       cgcgcctggggCCAAAGGGTAG‑CAAGACTCGGGCGTGACCGCCCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                          <  3:339061/143‑1 (MQ=255)
                                                                                                         cGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCa                                         >  2:17568/1‑150 (MQ=255)
                                                                                                            aCTGGGTAAAAGGGAAG‑AAAGACTGGGGAGTGACCGCGCACAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                      <  2:552818/150‑1 (MQ=255)
                                                                                                               gggTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAttt                                  >  2:1206320/1‑151 (MQ=255)
                                                                                                                 gTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                >  3:242439/1‑151 (MQ=255)
                                                                                                                    aaaGGGTAG‑CAAGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTa                               <  1:907349/149‑1 (MQ=255)
                                                                                                                     aaGGGTAG‑CAAGACTGGGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTa                               <  3:144449/148‑1 (MQ=255)
                                                                                                                     aaGGGTAACCAAGTCTGCGCCGGGCCCGCTCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGCGCAATAATGTCt                                <  2:360370/148‑1 (MQ=255)
                                                                                                                       gggTAG‑CAAGCCGGCGGCGTGCCCGCTCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                           <  2:683784/150‑1 (MQ=255)
                                                                                                                       gggTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCg                          >  2:19369/1‑151 (MQ=255)
                                                                                                                          tAG‑CAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtt                        <  1:328430/150‑1 (MQ=255)
                                                                                                                            agaaaGTCTGCGGCGTACCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtttt                      <  3:83589/148‑1 (MQ=255)
                                                                                                                               aaGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGc                   >  2:623706/1‑151 (MQ=255)
                                                                                                                               aaGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTg                    <  2:944706/150‑1 (MQ=255)
                                                                                                                               aaGACTGCGGCGTGACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                     <  2:99323/149‑1 (MQ=255)
                                                                                                                                 gACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                 <  1:232402/151‑1 (MQ=255)
                                                                                                                                  aCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                 <  1:812630/150‑1 (MQ=255)
                                                                                                                                     gcggcgTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt              <  1:1211151/150‑1 (MQ=255)
                                                                                                                                     gcggcgTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATg             <  1:716843/151‑1 (MQ=255)
                                                                                                                                        gcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTa           >  2:572441/1‑150 (MQ=255)
                                                                                                                                        gcgTGACCGCCCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGt            <  2:1273260/149‑1 (MQ=255)
                                                                                                                                          gTGCCCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACg        <  2:1057779/151‑1 (MQ=255)
                                                                                                                                          gTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACg        <  4:345129/151‑1 (MQ=255)
                                                                                                                                             aCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc     >  2:477490/1‑151 (MQ=255)
                                                                                                                                              ccGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACgg       <  3:213339/148‑1 (MQ=255)
                                                                                                                                                gcgcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAgg  >  2:629137/1‑151 (MQ=255)
                                                                                                                                                gcgcTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGc     <  2:84693/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAG‑CAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGG  >  NC_000913/3177600‑3177893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑