Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	44.4%	85.0 / 73.0	90	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/45);  new base T (40/0);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 4.08e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206286‑3206584                                                                                                                                                       |                                                                                                                                                       aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTcc                                                                                                                                                       <  1:612020/151‑1 (MQ=255)  gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCa                                                                                                                                                      <  2:499046/151‑1 (MQ=255)      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGAc                                                                                                                                                   <  1:848748/150‑1 (MQ=255)         tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACaaa                                                                                                                                                <  1:565735/150‑1 (MQ=255)           gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAAATTTTTTATACaaaaa                                                                                                                                              >  2:56237/1‑148 (MQ=255)            cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACa                                                                                                                                            <  3:290741/151‑1 (MQ=255)            cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACa                                                                                                                                            <  2:1071181/151‑1 (MQ=255)               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACaaaa                                                                                                                                         <  2:671148/151‑1 (MQ=255)                  aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGATAAATACaaaaaa                                                                                                                                       >  3:351065/1‑149 (MQ=255)                  aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACt                                                                                                                                      <  1:119558/151‑1 (MQ=255)                    tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAT‑gg                                                                                                                                   >  1:632643/1‑151 (MQ=255)                       cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGt                                                                                                                                <  2:289518/151‑1 (MQ=255)                           cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTAGACAAATAAAAAAACT‑GGAGTTg                                                                                                                              >  4:9800/1‑149 (MQ=255)                             cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCAACAAATAAAAAAAAT‑GGGGtt                                                                                                                               >  2:494011/1‑146 (MQ=255)                             cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCCGAAAAAAAAAAAAAAT‑GGAATTTcc                                                                                                                            >  1:95041/1‑149 (MQ=255)                              tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTAGACAAATAAAAAAAAT‑GGAGGTggc                                                                                                                            >  4:159731/1‑146 (MQ=255)                               gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatga                                                                                                                        <  2:991222/151‑1 (MQ=255)                               gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatg                                                                                                                         <  2:443819/150‑1 (MQ=255)                               gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatg                                                                                                                         <  2:137044/150‑1 (MQ=255)                                 tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAACA‑GGAGGTGGCatgat                                                                                                                       >  1:757685/1‑150 (MQ=255)                                 tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAT‑TGAg                                                                                                                                 >  1:290144/1‑140 (MQ=255)                                 tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAAATTTTTCAGAAAAAAAAAAAAAAT‑TGTGTTGTCat                                                                                                                          >  3:144591/1‑147 (MQ=255)                                  ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatgat                                                                                                                       <  4:310223/149‑1 (MQ=255)                                  ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatgat                                                                                                                       <  1:1036778/149‑1 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCACACAAATACAAAAAAT‑GGAGTTGCCATGATGAgg                                                                                                                   >  1:52066/1‑150 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCAtgatga                                                                                                                     <  3:349512/149‑1 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAg                                                                                                                    <  2:623028/150‑1 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGc                                                                                                                   <  2:692224/151‑1 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGc                                                                                                                   <  1:17074/151‑1 (MQ=255)                                      cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGa                                                                                                                 <  2:998390/151‑1 (MQ=255)                                        cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGaaa                                                                                                               <  1:922211/151‑1 (MQ=255)                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTCGACAAAAAAAAAAAAT‑GGAGTTTGCATGGTGAggg                                                                                                                  >  2:82799/1‑145 (MQ=255)                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAg                                                                                                              <  2:331278/151‑1 (MQ=255)                                           tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCATACAAAAAAAAAAAAA‑GGAGTTGGCattattggaggaa                                                                                                               >  1:880385/1‑140 (MQ=255)                                                attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTTAGACCAAAAAAAAAAAA‑AGAGTTTCCCTGATGAGCGCAAGaaa                                                                                                           >  1:287446/1‑147 (MQ=255)                                                   cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAAT‑AGAGTTGCCATGATGAGCGGAAGtaataaaaa                                                                                                     >  1:458714/1‑147 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                    <  2:865389/149‑1 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                     <  2:129576/148‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                  <  1:743530/150‑1 (MQ=255)                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑CGAAATTTTTCAAAAAAATAAAAAAAAA‑GGAGGTGTCATGGTTGGTGAAAAGAATAAACaa                                                                                                    >  1:586578/1‑148 (MQ=255)                                                       ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAATACAAAAAAT‑GGAGTTGCCATGATTGGGGAAAGAAATAAacaaca                                                                                                  >  4:57917/1‑149 (MQ=255)                                                         ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAGCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAAAAAAAAAAAAAAAT‑GGAGTTTCCATGATGGGGGAAAGTAATAAACaa                                                                                                    >  2:15256/1‑145 (MQ=255)                                                           cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg                                                                                             <  2:1039116/150‑1 (MQ=255)                                                             cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAAAAAAAACAAAAAAA‑GGAGTTTTCACGATGAGCGaaaagaaaaaacaaaa                                                                                                  >  2:87124/1‑130 (MQ=255)                                                              gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAACT‑GGGGTTGCCATGATGAGCGGAAGAAATAAACAAAAAGCAGTg                                                                                           >  1:329125/1‑149 (MQ=255)                                                              gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                          <  1:1007134/150‑1 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCAAACAAAAAAAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGGAATAAACAAAAAGCAGAGaa                                                                                         >  3:343115/1‑149 (MQ=255)                                                                 gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                      <  1:82813/151‑1 (MQ=255)                                                                    tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                    <  1:1154411/150‑1 (MQ=255)                                                                     cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAACAAAAAAAAAAACT‑TGGGTTGGCCTTATTAGGGGAAATAAAAAAAAACAAGCAAGGAATAAGtt                                                                                   >  3:428904/1‑150 (MQ=255)                                                                      aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAATACAAAAAAT‑GGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGtt                                                                                   >  4:394451/1‑149 (MQ=255)                                                                       cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAAAAAAAAAT‑TGAGTTGGCCTGATGAGGGAAAGAAATAAAGAACAGGCAGGGAATaa                                                                                      >  2:361373/1‑145 (MQ=255)                                                                        gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGACAAAAACAAAAAAA‑CGAGGTGCCCTGATGAGCGGAAGAAAAAAACAACAAGCAGGGAAAAAATTTAc                                                                                >  1:492839/1‑150 (MQ=255)                                                                          aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCAGACAAAAACAAAAAAT‑GGAGTTGCCCTGATGAGCGAAAGTAAAAAACAACAAGCAGGGAATAAggt                                                                                   >  3:212184/1‑143 (MQ=255)                                                                           cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAT‑GGAGTTGGCATGATGAGGGAAAGTAATAAACAAAAAGCAATGAATAAATTGACa                                                                               >  3:313842/1‑148 (MQ=255)                                                                              ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAAT‑GGAGGTGGCATTATGAGCGGAAGGAATAAACAAAAAGCAGTGAAAAAGATGACag                                                                              >  1:700632/1‑146 (MQ=255)                                                                               tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCCGACAAAAAAAAAAAAA‑GGGGTTGGCATGATGGGGGAAAGAAATAAACAAAAAGCAGAGAAAAAAGTGGCAgagaat                                                                         >  1:197394/1‑148 (MQ=255)                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGGAATTTTTCC‑GACAAATACAAAAACT‑AGAGATTCCATGATTAGGGGAAGTAATAAACAACAAGGAGGGAATAAGATGGCAGAGAgtgt                                                                       >  1:1110355/1‑151 (MQ=255)                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                       <  1:1114531/151‑1 (MQ=255)                                                                                 aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                      <  2:179902/151‑1 (MQ=255)                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                     <  2:972465/150‑1 (MQ=255)                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                     <  2:1011944/150‑1 (MQ=255)                                                                                    aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                   <  1:1163345/151‑1 (MQ=255)                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                  <  1:559064/151‑1 (MQ=255)                                                                                        atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                               <  2:1146919/151‑1 (MQ=255)                                                                                          tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAACAAAAAAAAAAAATGGGGGTTGCC‑TGATGGGCGGAAGGAATAAAAAACAAGCAGTGAATAAGGTGACAGAGAATGTCGCTAACttt                                                             >  3:315006/1‑151 (MQ=255)                                                                                              ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAAAAAAAAAAAAAAAA‑GGAGTTTGCATGATGGGCGGAAATAAAAAACAACAAGGAGTGAATAAATTTAAAGAGATTGTTGCTAAATTTaacc                                                         >  3:195933/1‑148 (MQ=255)                                                                                              ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGGCTTTTTTTAAACAAAATTAAAAAAA‑TGAGTTGGCATTTTTGGCGGCAGGAATAATAAAAAAGCAAAGAATAACATGGTAGAGGTTGTGGCTAACtt                                                              >  2:988558/1‑146 (MQ=255)                                                                                                 ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                       <  1:1090625/150‑1 (MQ=255)                                                                                                 ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAACAAAAAAAAAAAAT‑GGAGGTTGCATGATGGGCGGAAAGAATAAACAAAAAGGAGTGAAAAAAATTACAGAGAATTTTGCTAAACTTTCCGcc                                                       >  3:276005/1‑150 (MQ=255)                                                                                                   cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCAt                                                     <  3:291977/150‑1 (MQ=255)                                                                                                    aaaaaaTAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                    <  2:595396/150‑1 (MQ=255)                                                                                                       aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCAAACAAAAAAAAAAAAT‑GGAGGTGCCATGATGAGCGAAAGTAATAAACAAAAAGCAGGGAATAAGTTGACAGAGATTTTTGCTAAATTTTTCGCCCTGAttt                                                >  3:438442/1‑151 (MQ=255)                                                                                                        aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCCAACAAAAACAAAAACA‑GGGGTTGCCCTGATGAGCGGAAGTAATAAACAAAAGGCAGGGGAAAAATTGGCAGAGAATTTTGCTAATTTTTTCGCCCCGAttt                                                >  1:508591/1‑150 (MQ=255)                                                                                                         atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAAT‑GGAGTTGGCATGATGAGCGAAAGGAATAAGCAAAAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAAAATTTCCGCCCTGATTTCt                                              >  3:76717/1‑151 (MQ=255)                                                                                                           ataAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAATACAAAAAAT‑GGAGTTGCCATGATGAGGGGAAGTAATAAGAAACAAGCAGTGAAAAAGATGAAAGAGATTGTCGCTAACTTTAACGCCATGAttttt                                              >  2:30436/1‑147 (MQ=255)                                                                                                               aaCCACATTTTTAGTGGTAGTTTGTGGCGG‑TTGATTTTTTCCGACAAAAACAAAAAAT‑GGAGTTGTCATGATGGGTGAAAAGAATAAACAAAAAGCAGGGAATAAAGTGACAAAGATTGTTGCTAACTTTTTCGCCCTGATTTTTAccc                                          >  2:107081/1‑149 (MQ=255)                                                                                                               aaCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAACA‑GGAGGTGGCCTGATGAGCGGAAGAAATAAACAAAAAGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTACCCCCATGATTTCTAccc                                          >  1:324598/1‑149 (MQ=255)                                                                                                                 ccACATTTTTAGTGGTAGTTGGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                                      <  2:782470/151‑1 (MQ=255)                                                                                                                       ttttAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAACAAAAACAAAAAAA‑GGGGTTGCCATGGTGAGCGAAAAGAATAAACAAAAAGCAGTGAATAAAATGACAGAGATTGTCGCTAACTTTTCCCCCCTGATTTTTACCCCAAAGCCtg                                 >  2:1177075/1‑150 (MQ=255)                                                                                                                         ttAGTGGTAGTTTGGGGCGG‑TAAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACATAGATTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCtgat                               <  2:1141531/150‑1 (MQ=255)                                                                                                                              ggTAGTTTGGGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCACCGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                          <  1:500687/150‑1 (MQ=255)                                                                                                                                   tttGTGGCGG‑TGATTTTTCCCAGACAAATACAAAAACT‑GAAGTTGCCATGATGAGCAAAAGTAAAAACAAACAGGCAGTAAATAATTTAACAGAGATTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggta                      <  2:155787/149‑2 (MQ=255)                                                                                                                                   tttGTGCCGA‑TGAATTTTTCCAGACAAATAAAAAAACT‑GGAGTTGCCATGAGGACCAAAAATAAAAAGCAACGGACAGTGAATAGTTTAACAGAAATTGCCGCAACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                            <  1:214617/143‑1 (MQ=255)                                                                                                                                      gtgGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGCCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                  <  1:56246/150‑1 (MQ=255)                                                                                                                                       tgGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                 >  1:1158897/1‑150 (MQ=255)                                                                                                                                         gCGG‑TAAATTTTTCCAGAAAAATACAAAAACT‑GGAGTTGCCATGATGAGCAAAAGAAAAAAGCAACAGGCAGTGAATAAGTTGACAGATGTTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc               <  2:757532/150‑1 (MQ=255)                                                                                                                                              tGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa           >  3:272208/1‑150 (MQ=255)                                                                                                                                                   ttttCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt     >  2:943080/1‑151 (MQ=255)                                                                                                                                                   ttttCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt     >  1:328413/1‑151 (MQ=255)                                                                                                                                                   ttttCCAAAAAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc      <  2:1119060/150‑1 (MQ=255)                                                                                                                                                       ccAGAAAAATAAAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGGAGTGAATAAGTTGACAGAAATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  2:944369/150‑1 (MQ=255)                                                                                                                                                       ccAGAAAAATAAAAAAACT‑GGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  4:34869/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                       AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206286‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑