Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 400,157 T→C Y256C (TAC→TGC)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,1570TC100.0% 417.8 / NA 103Y256C (TAC→TGC) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/55);  total (48/55)

Only 100 of 103 total aligned reads displayed.

AAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACG  >  NC_000913/400010‑400303
                                                                                                                                                   |                                                                                                                                                  
aaaaaCACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAgg                                                                                                                                                 <  2:266001/151‑1 (MQ=255)
aaaaaCACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAgg                                                                                                                                                 <  1:1256424/151‑1 (MQ=255)
   aaCACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCAt                                                                                                                                              <  4:135945/151‑1 (MQ=255)
        gTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGa                                                                                                                                           >  3:46618/1‑149 (MQ=255)
           tACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCg                                                                                                                                      >  1:181344/1‑151 (MQ=255)
            acacGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATc                                                                                                                                       <  1:916947/149‑1 (MQ=255)
            acacGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGc                                                                                                                                     >  1:213871/1‑151 (MQ=255)
               cgcgCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTgg                                                                                                                                  <  1:928023/151‑1 (MQ=255)
                gcgcCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGACCTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTgg                                                                                                                                  >  2:1152837/1‑150 (MQ=255)
                   ccATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGa                                                                                                                               <  2:1076397/150‑1 (MQ=255)
                    cATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGt                                                                                                                             <  2:409325/151‑1 (MQ=255)
                      tGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTAc                                                                                                                           >  2:286252/1‑151 (MQ=255)
                      tGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTAc                                                                                                                           >  1:104362/1‑151 (MQ=255)
                       gCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACg                                                                                                                          <  3:421586/151‑1 (MQ=255)
                          tGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGAtc                                                                                                                       >  1:468398/1‑151 (MQ=255)
                           gcgcATCCCAACGTTTGATAAGCCTGAGCGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGAtct                                                                                                                      >  1:468976/1‑151 (MQ=255)
                           gcgcATCCCAACGTTTGATAAGCCTGAACGTCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGAt                                                                                                                        <  1:787628/149‑1 (MQ=255)
                              cATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGAtctctc                                                                                                                   <  2:1244869/151‑1 (MQ=255)
                                tCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTcc                                                                                                                  >  1:280047/1‑150 (MQ=255)
                                 cccAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCca                                                                                                                 <  2:283121/150‑1 (MQ=255)
                                 cccAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCca                                                                                                                 <  1:314239/150‑1 (MQ=255)
                                  ccAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCcaca                                                                                                               <  2:330772/151‑1 (MQ=255)
                                  ccAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCcac                                                                                                                >  2:2079/1‑150 (MQ=255)
                                   cAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCcac                                                                                                                >  2:54769/1‑149 (MQ=255)
                                    aaCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAgg                                                                                                             <  2:1221622/151‑1 (MQ=255)
                                    aaCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAgg                                                                                                             >  2:146346/1‑151 (MQ=255)
                                     aCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAgg                                                                                                             >  2:632148/1‑150 (MQ=255)
                                     aCGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGt                                                                                                            >  2:1245222/1‑151 (MQ=255)
                                         ttGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCt                                                                                                         <  2:554534/150‑1 (MQ=255)
                                              aaGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCtt                                                                                                    <  1:1152322/150‑1 (MQ=255)
                                              aaGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCtt                                                                                                    <  2:296630/150‑1 (MQ=255)
                                                    gAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGAtt                                                                                             <  2:822224/151‑1 (MQ=255)
                                                    gAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGAtt                                                                                             <  4:219954/151‑1 (MQ=255)
                                                     aaCGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTg                                                                                            >  2:411246/1‑151 (MQ=255)
                                                       cGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTc                                                                                          <  1:451113/151‑1 (MQ=255)
                                                           aaTCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTg                                                                                      >  2:1253957/1‑151 (MQ=255)
                                                               gCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCa                                                                                  <  2:1123657/151‑1 (MQ=255)
                                                                 ccGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGa                                                                                <  1:1177500/151‑1 (MQ=255)
                                                                    gATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACt                                                                             >  1:129861/1‑151 (MQ=255)
                                                                          aTCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCa                                                                        <  1:274518/150‑1 (MQ=255)
                                                                             gTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCg                                                                    <  2:153572/151‑1 (MQ=255)
                                                                              ttGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCg                                                                    >  1:463367/1‑150 (MQ=255)
                                                                                gATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAtc                                                                 <  2:1255434/151‑1 (MQ=255)
                                                                                gATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGAt                                                                  >  2:230940/1‑150 (MQ=255)
                                                                                    tCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCa                                                              <  1:316639/150‑1 (MQ=255)
                                                                                    tCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCa                                                              <  1:936962/150‑1 (MQ=255)
                                                                                     cTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTAGTGAGTACGATCTCTCCTCAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATATCa                                                              <  2:24407/149‑1 (MQ=255)
                                                                                      tGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                            >  1:1220022/1‑150 (MQ=255)
                                                                                       ggCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACg                                                            <  1:1242539/149‑1 (MQ=255)
                                                                                       ggCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                           <  2:856233/150‑1 (MQ=255)
                                                                                       ggCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                           <  2:834998/150‑1 (MQ=255)
                                                                                        gcgcAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGAcc                                                         >  2:983412/1‑151 (MQ=255)
                                                                                         cgcAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCt                                                        <  1:1244818/151‑1 (MQ=255)
                                                                                          gcAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCtt                                                       >  4:113431/1‑151 (MQ=255)
                                                                                               ggCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                   <  1:1096690/150‑1 (MQ=255)
                                                                                               ggCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                  <  1:1153047/151‑1 (MQ=255)
                                                                                               ggCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATc                                                  >  2:762427/1‑151 (MQ=255)
                                                                                                   gCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCtt                                              <  2:1264803/151‑1 (MQ=255)
                                                                                                     tggAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                            >  3:96561/2‑151 (MQ=255)
                                                                                                     cGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                            >  1:1209420/1‑151 (MQ=255)
                                                                                                      ggAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCt                                           <  4:270052/151‑1 (MQ=255)
                                                                                                       gAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTc                                          <  4:345909/151‑1 (MQ=255)
                                                                                                        aaCTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                         <  2:305756/151‑1 (MQ=255)
                                                                                                         aCTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                         >  4:372324/1‑150 (MQ=255)
                                                                                                          cTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                       >  3:85402/1‑151 (MQ=255)
                                                                                                            aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                       <  3:12860/149‑1 (MQ=255)
                                                                                                            aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                       <  1:687981/149‑1 (MQ=255)
                                                                                                            aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACg                                      >  3:147077/1‑150 (MQ=255)
                                                                                                              cACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                   >  2:1247426/1‑151 (MQ=255)
                                                                                                               aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGAt                                    >  3:216151/1‑149 (MQ=255)
                                                                                                               aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                  <  2:941191/151‑1 (MQ=255)
                                                                                                                 tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACt                                >  1:361892/1‑151 (MQ=255)
                                                                                                                 tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACt                                >  2:161138/1‑151 (MQ=255)
                                                                                                                  ttCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACt                                <  2:163908/150‑1 (MQ=255)
                                                                                                                   tCGCGCCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACtt                               <  1:1096102/150‑1 (MQ=255)
                                                                                                                   tCGCGCCATCTTCGTCAATGTACTTGGCGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACttt                              >  1:310186/1‑151 (MQ=255)
                                                                                                                      cgcCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                           >  2:1179482/1‑151 (MQ=255)
                                                                                                                       gcCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                           <  1:100520/118‑1 (MQ=255)
                                                                                                                       gcCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGa                                                           >  2:100506/1‑118 (MQ=255)
                                                                                                                       gcCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGa                          <  1:497086/151‑1 (MQ=255)
                                                                                                                       gcCATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGa                          <  2:1048756/151‑1 (MQ=255)
                                                                                                                        ccATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAt                         <  1:244544/151‑1 (MQ=255)
                                                                                                                         cATCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtc                        >  2:1128142/1‑151 (MQ=255)
                                                                                                                           tCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                       >  2:694450/1‑150 (MQ=255)
                                                                                                                           tCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                       >  4:297759/1‑150 (MQ=255)
                                                                                                                           tCTTCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcg                       >  1:954041/1‑150 (MQ=255)
                                                                                                                              tCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaa                     <  2:162968/149‑1 (MQ=255)
                                                                                                                              tCGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcga                      <  2:770152/148‑1 (MQ=255)
                                                                                                                               cGTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactc                  <  1:841340/151‑1 (MQ=255)
                                                                                                                                gTCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                 >  3:148797/1‑151 (MQ=255)
                                                                                                                                 tCAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                 >  3:150174/1‑150 (MQ=255)
                                                                                                                                  cAATGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcga                <  3:402012/150‑1 (MQ=255)
                                                                                                                                    aTGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACg             >  4:336099/1‑151 (MQ=255)
                                                                                                                                    aTGTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACg             >  4:36102/1‑151 (MQ=255)
                                                                                                                                      gTACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGc            <  2:468311/150‑1 (MQ=255)
                                                                                                                                       tACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc           >  3:56155/1‑150 (MQ=255)
                                                                                                                                       tACTTGGTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc           >  2:1026910/1‑150 (MQ=255)
                                                                                                                                            ggTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc      <  2:587333/150‑1 (MQ=255)
                                                                                                                                            ggTGTCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCg     <  1:736065/151‑1 (MQ=255)
                                                                                                                                               gtCGCAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACg  >  1:162254/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACG  >  NC_000913/400010‑400303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑