Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,382,351 C→T intergenic (‑33/‑292) frdA ← / → epmA anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit/Elongation Factor P Lys34 lysyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,382,3510CT99.3% 550.1 / NA 142intergenic (‑33/‑292)frdA/epmAanaerobic fumarate reductase catalytic and NAD/flavoprotein subunit/Elongation Factor P Lys34 lysyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (77/64);  total (78/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATCACT  >  NC_000913/4382204‑4382498
                                                                                                                                                   |                                                                                                                                                   
 ttttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGt                                                                                                                                                 >  4:180592/1‑151 (MQ=255)
 ttttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGt                                                                                                                                                 >  1:52438/1‑151 (MQ=255)
 ttttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGt                                                                                                                                                 >  1:94575/1‑151 (MQ=255)
   ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTa                                                                                                                                                <  2:789332/150‑1 (MQ=255)
   ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTAc                                                                                                                                               >  2:225250/1‑151 (MQ=255)
    tGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTAc                                                                                                                                               <  1:959521/150‑1 (MQ=255)
        aTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTc                                                                                                                                            >  1:67820/1‑149 (MQ=255)
        aTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAg                                                                                                                                          <  1:982611/151‑1 (MQ=255)
          tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACCTTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAggg                                                                                                                                        >  3:8719/1‑151 (MQ=255)
          tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAggg                                                                                                                                        >  1:556830/1‑151 (MQ=255)
          tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAggg                                                                                                                                        >  2:287478/1‑151 (MQ=255)
              gCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTa                                                                                                                                      >  2:104157/1‑149 (MQ=255)
              gCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTa                                                                                                                                      >  2:587131/1‑149 (MQ=255)
                 aTTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTAc                                                                                                                                 <  2:148568/151‑1 (MQ=255)
                 aTTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGGTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTAc                                                                                                                                 >  2:468243/1‑151 (MQ=255)
                      tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGa                                                                                                                             <  1:145604/150‑1 (MQ=255)
                         aTTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGt                                                                                                                         >  1:908338/1‑151 (MQ=255)
                             ggATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACg                                                                                                                     >  2:1148272/1‑151 (MQ=255)
                              gATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACg                                                                                                                     >  3:321799/1‑150 (MQ=255)
                               aTTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGt                                                                                                                   >  4:45546/1‑151 (MQ=255)
                                tttGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCCGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGt                                                                                                                   >  2:951956/1‑150 (MQ=255)
                                tttGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGt                                                                                                                   >  2:1235534/1‑150 (MQ=255)
                                  tGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCt                                                                                                                 <  1:1144917/150‑1 (MQ=255)
                                  tGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCt                                                                                                                 <  1:858758/150‑1 (MQ=255)
                                   gCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGc                                                                                                               <  2:391852/151‑1 (MQ=255)
                                       gcgcGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGaa                                                                                                            <  4:375794/150‑1 (MQ=255)
                                        cgcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACg                                                                                                          <  2:379219/151‑1 (MQ=255)
                                           ggCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTa                                                                                                       <  1:651076/151‑1 (MQ=255)
                                                 aaTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCAc                                                                                                 <  4:238327/151‑1 (MQ=255)
                                                  aTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCAc                                                                                                 >  4:324713/1‑150 (MQ=255)
                                                  aTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACt                                                                                                <  3:88355/151‑1 (MQ=255)
                                                  aTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACt                                                                                                <  1:432482/151‑1 (MQ=255)
                                                  aTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACt                                                                                                <  1:403154/151‑1 (MQ=255)
                                                  aTTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACt                                                                                                <  2:1180740/151‑1 (MQ=255)
                                                   ttGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTg                                                                                               >  1:1036160/1‑151 (MQ=255)
                                                      cagcaCGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTc                                                                                            <  1:919393/151‑1 (MQ=255)
                                                      cagcaCGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTc                                                                                            >  3:377762/1‑151 (MQ=255)
                                                        gcaCGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCt                                                                                          <  2:1046887/151‑1 (MQ=255)
                                                        gcaCGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCt                                                                                          <  3:130396/151‑1 (MQ=255)
                                                           cGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTta                                                                                       <  1:932230/151‑1 (MQ=255)
                                                              aaTCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTtat                                                                                      >  1:154704/1‑149 (MQ=255)
                                                              aaTCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAg                                                                                    <  4:383023/151‑1 (MQ=255)
                                                               aTCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAgg                                                                                   <  1:694654/151‑1 (MQ=255)
                                                                 cccGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGCAAGGTTTGCACGACATTCCTCCCGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGt                                                                                  >  3:154576/1‑150 (MQ=255)
                                                                 cccGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGt                                                                                  >  1:117967/1‑150 (MQ=255)
                                                                 cccGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGt                                                                                  <  3:134555/150‑1 (MQ=255)
                                                                 cccGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTa                                                                                 <  4:451756/151‑1 (MQ=255)
                                                                  ccGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTac                                                                                >  1:84324/1‑151 (MQ=255)
                                                                    gcgcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTaca                                                                               >  1:1031854/1‑150 (MQ=255)
                                                                    gcgcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTaca                                                                               >  1:569670/1‑150 (MQ=255)
                                                                      gcCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTacaaca                                                                            <  1:179905/151‑1 (MQ=255)
                                                                        cACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGt                                                                          <  2:750275/151‑1 (MQ=255)
                                                                          ccGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGtat                                                                        <  4:430179/151‑1 (MQ=255)
                                                                           cGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGtata                                                                       <  3:120251/151‑1 (MQ=255)
                                                                           cGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGAGTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGGACAAAAGta                                                                         >  1:529008/1‑149 (MQ=255)
                                                                            ggCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGtat                                                                        >  1:1026249/1‑149 (MQ=255)
                                                                               gcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTc                                                                    >  2:1212836/1‑150 (MQ=255)
                                                                               gcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCt                                                                   <  3:387267/151‑1 (MQ=255)
                                                                               gcCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCt                                                                   >  2:975402/1‑151 (MQ=255)
                                                                                      aTGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGAt                                                            >  1:110650/1‑151 (MQ=255)
                                                                                         gCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAg                                                         <  2:814368/151‑1 (MQ=255)
                                                                                           aaGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTc                                                       <  4:426678/151‑1 (MQ=255)
                                                                                              aTCGGCTTGGAAGGGTTGGAAGCCAATCCTCCAAATTTTTTTTATCCCACACCCACGTTCTTCAGGGAAAGTACCTGAAAGTTACGGTCTGCGAAAGCTATTCCACTGCTCCTTTATAAGTACAACACTATAGTCTGGTGGATaa                                                          >  2:27685/1‑145 (MQ=255)
                                                                                                cGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGaaa                                                  <  1:624395/151‑1 (MQ=255)
                                                                                                cGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGaa                                                   <  3:88008/150‑1 (MQ=255)
                                                                                                 ggCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAt                                                 >  1:492143/1‑151 (MQ=255)
                                                                                                   cTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAttt                                               >  2:1104079/1‑151 (MQ=255)
                                                                                                     tGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAAttt                                               <  3:281378/149‑1 (MQ=255)
                                                                                                     tGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTg                                              >  2:393456/1‑150 (MQ=255)
                                                                                                      gAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGa                                             <  2:908162/150‑1 (MQ=255)
                                                                                                      gAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGAc                                            >  2:1079898/1‑151 (MQ=255)
                                                                                                       aaaGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACg                                           <  2:727379/151‑1 (MQ=255)
                                                                                                         aGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACgag                                         <  1:898373/151‑1 (MQ=255)
                                                                                                          ggTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTACTTTATAGGTACAACAGTATAGTCTGATGGAGAAGTCTGAAATTTGACgaga                                        <  3:411018/151‑1 (MQ=255)
                                                                                                           gTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACg                                           >  1:464818/1‑147 (MQ=255)
                                                                                                           gTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACg                                           >  2:699293/1‑147 (MQ=255)
                                                                                                             ttGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATc                                      <  2:145420/150‑1 (MQ=255)
                                                                                                             ttGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATc                                      <  3:276374/150‑1 (MQ=255)
                                                                                                              tGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGATGAGATCg                                     >  1:244504/1‑150 (MQ=255)
                                                                                                               gCACGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAt                                   <  2:500550/151‑1 (MQ=255)
                                                                                                                 aCGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAttt                                 >  2:1146721/1‑151 (MQ=255)
                                                                                                                  cGACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAtttt                                >  1:747296/1‑151 (MQ=255)
                                                                                                                   gACATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGGCTGATGGATAAGTCTGAAATTTGACGAGATCGAttttt                               >  1:289949/1‑151 (MQ=255)
                                                                                                                     cATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAttttttt                             >  2:810068/1‑151 (MQ=255)
                                                                                                                     cATTCCTCCAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGAtttttgt                             <  2:459813/151‑3 (MQ=255)
                                                                                                                        tcctccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGt                          >  2:830203/1‑151 (MQ=255)
                                                                                                                        tcctccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGt                          >  1:1245567/1‑151 (MQ=255)
                                                                                                                            ccAGATTGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTgcgc                      >  1:793474/1‑151 (MQ=255)
                                                                                                                                 ttGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAag                   >  1:1183221/1‑149 (MQ=255)
                                                                                                                                  tGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAagag                 <  4:353982/150‑1 (MQ=255)
                                                                                                                                  tGTTTTTATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAgg                >  1:932121/1‑151 (MQ=255)
                                                                                                                                    tttttATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAggg               >  2:904481/1‑150 (MQ=255)
                                                                                                                                     ttttATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCt             >  1:519074/1‑151 (MQ=255)
                                                                                                                                      tttATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCt             <  4:378709/150‑1 (MQ=255)
                                                                                                                                       ttATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTa            <  3:427919/150‑1 (MQ=255)
                                                                                                                                       ttATCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTa            <  1:587245/150‑1 (MQ=255)
                                                                                                                                         aTCCCACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTaaa          <  2:551207/150‑1 (MQ=255)
                                                                                                                                           cccACAGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTa       <  2:875957/151‑1 (MQ=255)
                                                                                                                                               caGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATCAc   <  3:373784/151‑1 (MQ=255)
                                                                                                                                                aGCTACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATCACt  <  2:435374/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATAGGTACAACAGTATAGTCTGATGGATAAGTCTGAAATTTGACGAGATCGATTTTTTTAGTGCGCAAGAGGGCTAAATTATCACT  >  NC_000913/4382204‑4382498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: