Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,640,125 T→C N61S (AAC→AGC)  arcA ← response regulator in two‑component regulatory system with ArcB or CpxA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,640,1250TC99.1% 439.0 / NA 112N61S (AAC→AGC) arcAresponse regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (50/61);  minor base G (1/0);  total (51/61)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAAC  >  NC_000913/4639979‑4640271
                                                                                                                                                  |                                                                                                                                                  
gTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCtt                                                                                                                                                <  2:251871/151‑1 (MQ=255)
gTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCtt                                                                                                                                                <  2:556074/151‑1 (MQ=255)
 ttCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCtt                                                                                                                                                >  1:1279022/1‑150 (MQ=255)
 ttCACGCGGGATGAACGATTTGGTGATGTAGTCATTTGCACCGATTTCGAGGCCGAGGATTGTATCGAGTTCGTTGACACGGCCAGTCAGGAACATCAACGCAAAATTCGCCGGCTCGCGCAGTTCACGCGCTAACAGAAGACCGGTCtt                                                                                                                                                >  2:783571/1‑150 (MQ=255)
   cACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTAcc                                                                                                                                             >  1:69126/1‑151 (MQ=255)
     cgcgGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCg                                                                                                                                           <  4:347073/151‑1 (MQ=255)
     cgcgGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCg                                                                                                                                           <  1:25418/151‑1 (MQ=255)
      gcgGGTTGAACGGTTTGGTGATGTAGTAATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCgg                                                                                                                                          >  4:215830/1‑151 (MQ=255)
       cgGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGc                                                                                                                                         <  2:588520/151‑1 (MQ=255)
        gggTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCa                                                                                                                                        <  2:568531/151‑1 (MQ=255)
         ggTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAg                                                                                                                                       <  2:342088/151‑1 (MQ=255)
            tGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGAt                                                                                                                                     <  4:293634/150‑1 (MQ=255)
             gAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTg                                                                                                                                   >  1:694705/1‑151 (MQ=255)
             gAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTg                                                                                                                                   >  1:124593/1‑151 (MQ=255)
              aaCGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTg                                                                                                                                   >  1:835357/1‑150 (MQ=255)
               aCGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGat                                                                                                                                 <  2:797554/151‑1 (MQ=255)
                cGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGata                                                                                                                                <  1:566595/151‑1 (MQ=255)
                      ggTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCAt                                                                                                                           <  1:1113571/150‑1 (MQ=255)
                      ggTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCAt                                                                                                                           <  3:230680/150‑1 (MQ=255)
                      ggTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATg                                                                                                                          >  1:149291/1‑151 (MQ=255)
                        tGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGAt                                                                                                                        >  2:840120/1‑151 (MQ=255)
                         gATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGAt                                                                                                                        <  1:1125863/150‑1 (MQ=255)
                         gATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATc                                                                                                                       <  1:1239775/151‑1 (MQ=255)
                         gATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATc                                                                                                                       <  2:164481/151‑1 (MQ=255)
                           tGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCAc                                                                                                                     >  2:1033002/1‑151 (MQ=255)
                                tCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGt                                                                                                                >  2:628702/1‑151 (MQ=255)
                                 cATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGtt                                                                                                               <  4:229036/151‑1 (MQ=255)
                                  aTCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGtt                                                                                                               >  1:1014034/1‑150 (MQ=255)
                                  aTCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTg                                                                                                              >  4:104456/1‑151 (MQ=255)
                                      gCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTg                                                                                                              <  1:1049134/147‑1 (MQ=255)
                                      gCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTg                                                                                                              <  2:644208/147‑1 (MQ=255)
                                      gCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGa                                                                                                             <  1:1148439/148‑1 (MQ=255)
                                      gCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGa                                                                                                             <  4:106462/148‑1 (MQ=255)
                                       cACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTc                                                                                                         <  1:306069/151‑1 (MQ=255)
                                         ccGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCat                                                                                                       <  1:83429/151‑1 (MQ=255)
                                         ccGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCat                                                                                                       <  2:879933/151‑1 (MQ=255)
                                           gATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCatat                                                                                                     <  1:697590/151‑1 (MQ=255)
                                           gATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCatat                                                                                                     >  1:359102/1‑151 (MQ=255)
                                           gATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCatat                                                                                                     >  1:198495/1‑151 (MQ=255)
                                                cGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTc                                                                                                   >  3:265266/1‑148 (MQ=255)
                                                  aGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCag                                                                                                 >  2:1150666/1‑148 (MQ=255)
                                                  aGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCag                                                                                                 >  1:326869/1‑148 (MQ=255)
                                                  aGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCag                                                                                                 >  2:1245837/1‑148 (MQ=255)
                                                    gCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAgg                                                                                            <  1:204746/151‑1 (MQ=255)
                                                     ccGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAgg                                                                                            >  3:242477/1‑150 (MQ=255)
                                                        agaATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCt                                                                                        <  4:268286/151‑1 (MQ=255)
                                                         gaATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCt                                                                                        <  3:199245/150‑1 (MQ=255)
                                                         gaATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTg                                                                                       >  2:1125467/1‑151 (MQ=255)
                                                          aaTTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTg                                                                                       >  2:890469/1‑150 (MQ=255)
                                                          aaTTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTGGATGTCATATTCAGAGAGGATCTg                                                                                       >  1:645307/1‑150 (MQ=255)
                                                               tATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCa                                                                                  <  4:116024/150‑1 (MQ=255)
                                                                   gACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTcc                                                                             >  2:768894/1‑151 (MQ=255)
                                                                   gACTTCGTTGTCACGGCCAGTCAGAAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTcc                                                                             <  4:202655/151‑1 (MQ=255)
                                                                     cTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTcc                                                                             <  2:694134/149‑1 (MQ=255)
                                                                      ttCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCcgcg                                                                          >  1:767562/1‑151 (MQ=255)
                                                                       tCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCcgc                                                                           <  4:104928/149‑1 (MQ=255)
                                                                         gTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGcc                                                                        >  2:624035/1‑150 (MQ=255)
                                                                          ttGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCAt                                                                      >  1:681604/1‑151 (MQ=255)
                                                                               aCGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTc                                                                 >  1:1269906/1‑151 (MQ=255)
                                                                                 ggCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGc                                                               >  2:1178093/1‑151 (MQ=255)
                                                                                  gCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCt                                                              <  2:440635/151‑1 (MQ=255)
                                                                                     aGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCGCCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCg                                                           <  3:123100/151‑1 (MQ=255)
                                                                                       tCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGa                                                          >  3:255511/1‑150 (MQ=255)
                                                                                        cAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGaaa                                                        <  4:112463/151‑1 (MQ=255)
                                                                                        cAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGaa                                                         >  2:146927/1‑150 (MQ=255)
                                                                                         aGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGaaa                                                        >  1:848940/1‑150 (MQ=255)
                                                                                            aaCATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcat                                                    <  3:342231/151‑1 (MQ=255)
                                                                                            aaCATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcat                                                    <  3:328081/151‑1 (MQ=255)
                                                                                             aCATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcatc                                                   <  1:890644/151‑1 (MQ=255)
                                                                                             aCATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcatc                                                   >  4:153763/1‑151 (MQ=255)
                                                                                             aCATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcatc                                                   >  1:367477/1‑151 (MQ=255)
                                                                                               aTCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAAcatcat                                                 >  3:40000/1‑151 (MQ=255)
                                                                                                 cAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAg                                               <  2:851111/151‑1 (MQ=255)
                                                                                                    cGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCt                                            >  1:428726/1‑151 (MQ=255)
                                                                                                     gCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCtt                                           >  1:145663/1‑151 (MQ=255)
                                                                                                      cAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTc                                          >  2:290492/1‑151 (MQ=255)
                                                                                                       aaCATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTcc                                         >  2:18879/1‑151 (MQ=255)
                                                                                                        aCATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCg                                        <  2:1179615/151‑1 (MQ=255)
                                                                                                         cATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGc                                       <  2:766085/151‑1 (MQ=255)
                                                                                                          aTTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGc                                       <  3:210686/150‑1 (MQ=255)
                                                                                                            tCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCtt                                     <  2:395689/150‑1 (MQ=255)
                                                                                                             cGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTc                                    <  1:158789/150‑1 (MQ=255)
                                                                                                                cTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGaa                                 >  2:1004820/1‑150 (MQ=255)
                                                                                                                 tGCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGaaa                                >  2:21728/1‑150 (MQ=255)
                                                                                                                  gCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGaaaa                               <  4:192916/150‑1 (MQ=255)
                                                                                                                  gCTCGCGCAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAAt                              >  2:561057/1‑151 (MQ=255)
                                                                                                                       cgcAGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACttt                         >  1:865424/1‑151 (MQ=255)
                                                                                                                          aGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCa                      <  3:763/151‑1 (MQ=255)
                                                                                                                          aGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCa                      <  2:164604/151‑1 (MQ=255)
                                                                                                                          aGTTCACGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCa                      <  1:587401/151‑1 (MQ=255)
                                                                                                                               aCGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgtg                 >  1:100550/1‑151 (MQ=255)
                                                                                                                               aCGCGCTAACAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACgtg                 >  1:131982/1‑151 (MQ=255)
                                                                                                                                   gcTAACAGAAGACCGCTCTTACACGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGc             >  2:490579/1‑151 (MQ=255)
                                                                                                                                      aaCAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgtg          <  1:402285/151‑1 (MQ=255)
                                                                                                                                      aaCAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCgt           <  1:628829/150‑1 (MQ=255)
                                                                                                                                        cAGAAGACCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGtt        >  2:965750/1‑151 (MQ=255)
                                                                                                                                          gaagaCCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAc      >  1:335719/1‑151 (MQ=255)
                                                                                                                                          gaagaCCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAc      <  1:280833/151‑1 (MQ=255)
                                                                                                                                            agaCCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTAcc     >  2:442966/1‑150 (MQ=255)
                                                                                                                                              aCCGCTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAAc  <  1:955320/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACTTCGTTGTCACGGCCAGTCAGGAACATCAACGCAACATTCGCCTGCTCGCGCAGTTCACGCGCTAACAGAAGACCGTTCTTACCCGGCAGATTGATATCCATGATCACCAGGTTGATGTCATATTCAGAGAGGATCTGATGCATTTCCGCGCCATCTGTCGCTTCGAAAACATCATAGCCTTCCGCTTCGAAAATACTTTTCAACGTGTTGCGTGTTACCAAC  >  NC_000913/4639979‑4640271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: