Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 418,974 A→G A362A (GCA→GCG)  phoR → sensory histidine kinase in two‑component regulatory system with PhoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913418,9740AG99.2% 489.5 / ‑4.5 133A362A (GCA→GCG) phoRsensory histidine kinase in two‑component regulatory system with PhoB
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (63/69);  total (63/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 133 total aligned reads displayed.

CAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATATTGAGAGTACAGT  >  NC_000913/418827‑419120
                                                                                                                                                   |                                                                                                                                                  
  aCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGa                                                                                                                                               <  3:183839/151‑1 (MQ=255)
    gAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGc                                                                                                                                             >  3:47434/1‑151 (MQ=255)
     aaGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCa                                                                                                                                            <  3:167780/151‑1 (MQ=255)
     aaGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCa                                                                                                                                            <  4:330448/151‑1 (MQ=255)
      aGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCat                                                                                                                                           >  4:209493/1‑151 (MQ=255)
        aTCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCatat                                                                                                                                         <  4:183600/151‑1 (MQ=255)
          cAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATAtt                                                                                                                                        <  1:585290/150‑1 (MQ=255)
          cAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTc                                                                                                                                       >  2:671674/1‑151 (MQ=255)
           aGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTcc                                                                                                                                      <  2:353642/151‑1 (MQ=255)
           aGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTcc                                                                                                                                      >  2:676266/1‑151 (MQ=255)
           aGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTcc                                                                                                                                      <  1:1274731/151‑1 (MQ=255)
             cTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCcgc                                                                                                                                    >  1:827478/1‑151 (MQ=255)
             cTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCcgc                                                                                                                                    >  4:387199/1‑151 (MQ=255)
               aCGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCcgcg                                                                                                                                   <  4:160880/150‑1 (MQ=255)
               aCGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCcgc                                                                                                                                    <  1:885280/149‑1 (MQ=255)
               aCGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCgcgc                                                                                                                                  >  1:278273/1‑151 (MQ=255)
                cGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGcc                                                                                                                                 >  2:126642/1‑151 (MQ=255)
                 gCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCt                                                                                                                                <  1:1258949/151‑1 (MQ=255)
                 gCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCt                                                                                                                                <  1:266201/151‑1 (MQ=255)
                   aGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGa                                                                                                                              <  1:824965/151‑1 (MQ=255)
                    gTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGAc                                                                                                                             >  1:111859/1‑151 (MQ=255)
                     tGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGAcc                                                                                                                            >  2:1174464/1‑151 (MQ=255)
                      gCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCg                                                                                                                           >  1:145837/1‑151 (MQ=255)
                        gATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCg                                                                                                                           >  1:101574/1‑149 (MQ=255)
                        gATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCg                                                                                                                           >  4:77873/1‑149 (MQ=255)
                         aTTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGc                                                                                                                        <  3:387193/151‑1 (MQ=255)
                            tCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGt                                                                                                                      <  1:1068288/150‑1 (MQ=255)
                             cGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGttt                                                                                                                    >  2:146065/1‑151 (MQ=255)
                             cGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGttt                                                                                                                    >  1:382450/1‑151 (MQ=255)
                               aaCCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGttttt                                                                                                                  >  4:386928/1‑151 (MQ=255)
                                     gTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATcgcg                                                                                                            >  2:772672/1‑151 (MQ=255)
                                        tataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTg                                                                                                         >  3:116196/1‑151 (MQ=255)
                                         ataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTgg                                                                                                        >  3:413749/1‑150 (MQ=255)
                                         ataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTg                                                                                                         <  1:1166680/150‑1 (MQ=255)
                                         ataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTg                                                                                                         >  1:838826/1‑150 (MQ=255)
                                          taACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGAt                                                                                                       >  1:472538/1‑151 (MQ=255)
                                           aaCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCcgc                                                                                                                                    <  1:269174/121‑1 (MQ=255)
                                           aaCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATa                                                                                                      <  1:65811/151‑1 (MQ=255)
                                            aCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATaa                                                                                                     <  1:1092051/151‑1 (MQ=255)
                                            aCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATaa                                                                                                     <  3:70793/151‑1 (MQ=255)
                                            aCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATa                                                                                                      >  4:143652/1‑150 (MQ=255)
                                             cGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATaaa                                                                                                    <  2:97330/151‑1 (MQ=255)
                                             cGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATaaa                                                                                                    <  1:739386/151‑1 (MQ=255)
                                              gCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATaaa                                                                                                    <  3:297599/150‑1 (MQ=255)
                                                 gTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAgcgc                                                                                                <  2:145814/151‑1 (MQ=255)
                                                   gAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGcgcg                                                                                               <  4:60237/150‑1 (MQ=255)
                                                    aaTCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGtt                                                                                             >  2:1167709/1‑151 (MQ=255)
                                                    aaTCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGtt                                                                                             >  2:794369/1‑151 (MQ=255)
                                                      tCATACGCCGGCAGGCACACATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGCATTTAGCGTTGAAGGTAACGGACCGGGCATTGCGCCGGAGCATCTTCCGCGCCTGACCGACCGTTTTTATCGCGTTGCTAAAGCGCGTTcc                                                                                           >  2:488491/1‑151 (MQ=255)
                                                      tCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTcc                                                                                           >  3:267018/1‑151 (MQ=255)
                                                          aCGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGc                                                                                       <  1:202417/151‑1 (MQ=255)
                                                          aCGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGc                                                                                       <  2:462248/151‑1 (MQ=255)
                                                           tgCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGc                                                                                       >  2:932988/2‑150 (MQ=255)
                                                            gCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCa                                                                                      >  1:711166/1‑150 (MQ=255)
                                                              cGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCaaa                                                                                    >  2:299459/1‑150 (MQ=255)
                                                              cGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAAc                                                                                   >  1:780831/1‑151 (MQ=255)
                                                               ggAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAAcc                                                                                  <  2:863102/151‑1 (MQ=255)
                                                                gAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACcg                                                                                 >  1:628338/1‑151 (MQ=255)
                                                                gAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACcg                                                                                 <  2:1097417/151‑1 (MQ=255)
                                                                   ggCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACcggcg                                                                              <  2:111841/151‑1 (MQ=255)
                                                                      aCGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTa                                                                           <  1:224517/151‑1 (MQ=255)
                                                                       cGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGTGCGTTCCCGGCAAACCGGCGGTAg                                                                          <  2:1117168/151‑1 (MQ=255)
                                                                            atCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGa                                                                      <  3:411639/150‑1 (MQ=255)
                                                                            atCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGAt                                                                     >  2:392636/1‑151 (MQ=255)
                                                                            atCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGAt                                                                     <  1:852515/151‑1 (MQ=255)
                                                                             tCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGAtt                                                                    >  1:1243580/1‑151 (MQ=255)
                                                                              cACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGAtt                                                                    >  2:307077/1‑150 (MQ=255)
                                                                                  gTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAggg                                                                >  4:24500/1‑150 (MQ=255)
                                                                                    aCGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGtt                                                              <  2:727847/150‑1 (MQ=255)
                                                                                    aCGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGtt                                                              <  4:347160/150‑1 (MQ=255)
                                                                                      gCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTa                                                             >  2:590126/1‑149 (MQ=255)
                                                                                         ggCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCg                                                          <  1:775826/149‑1 (MQ=255)
                                                                                         ggCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGa                                                         <  1:706098/150‑1 (MQ=255)
                                                                                            aGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGt                                                     >  4:84384/1‑151 (MQ=255)
                                                                                              cGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTg                                                    >  4:294358/1‑150 (MQ=255)
                                                                                                      gCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCt                                           >  1:1253869/1‑151 (MQ=255)
                                                                                                      gCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCt                                           <  1:597447/151‑1 (MQ=255)
                                                                                                      gCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCt                                           <  1:488899/151‑1 (MQ=255)
                                                                                                       cACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCtg                                          >  1:192898/1‑151 (MQ=255)
                                                                                                        aCGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCtgt                                         >  1:76183/1‑151 (MQ=255)
                                                                                                         cGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCtgt                                         <  3:163797/150‑1 (MQ=255)
                                                                                                          ggTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGa                                       <  2:827310/151‑1 (MQ=255)
                                                                                                          ggTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGa                                       <  1:544336/151‑1 (MQ=255)
                                                                                                           gTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGaa                                      <  2:641966/151‑1 (MQ=255)
                                                                                                              ccGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAatca                                   <  4:178769/151‑1 (MQ=255)
                                                                                                              ccGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAatc                                    >  2:706617/1‑150 (MQ=255)
                                                                                                                  aTTTAGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCAc                               <  1:853104/151‑1 (MQ=255)
                                                                                                                      aGCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGaa                            >  2:1038505/1‑150 (MQ=255)
                                                                                                                       gCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGaa                            >  3:446419/1‑149 (MQ=255)
                                                                                                                       gCGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAg                          <  1:1220802/151‑1 (MQ=255)
                                                                                                                        cGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAat                                     >  1:829654/1‑139 (MQ=255)
                                                                                                                        cGTTGAAGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAat                                     <  2:829486/139‑1 (MQ=255)
                                                                                                                              aGATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTg                   >  4:13863/1‑151 (MQ=255)
                                                                                                                               gATAACGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGa                  <  1:150230/151‑1 (MQ=255)
                                                                                                                                    cGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATAtt             >  1:673506/1‑151 (MQ=255)
                                                                                                                                    cGGACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATAtt             <  2:13655/151‑1 (MQ=255)
                                                                                                                                      gACCGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTAGCCTGAATATTga           <  1:1102184/151‑1 (MQ=255)
                                                                                                                                         cGGGCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATATTGagag        <  2:594232/151‑1 (MQ=255)
                                                                                                                                          gggCATTGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATATTGagag        >  3:241128/1‑150 (MQ=255)
                                                                                                                                                tGCGCCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATATTGAGAGTACAGt  <  1:974903/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGTTCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCATCACGAAAGTCGCCTGAATATTGAGAGTACAGT  >  NC_000913/418827‑419120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑