Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 451,552 A→G V20A (GTA→GCA)  cyoA ← cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913451,5520AG100.0% 387.7 / NA 99V20A (GTA→GCA) cyoAcytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/64);  total (35/64)

ACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAA  >  NC_000913/451406‑451698
                                                                                                                                                  |                                                                                                                                                  
aCTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGt                                                                                                                                                >  1:292868/1‑151 (MQ=255)
   tCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTg                                                                                                                                               <  2:343752/149‑1 (MQ=255)
   tCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGc                                                                                                                                              <  2:264639/150‑1 (MQ=255)
   tCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGc                                                                                                                                              <  1:183374/149‑1 (MQ=255)
     cAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTg                                                                                                                                           >  1:566045/1‑151 (MQ=255)
       ggCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCa                                                                                                                                         <  1:850318/151‑1 (MQ=255)
       ggCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGAAGTGCCTGCa                                                                                                                                         <  4:93339/151‑1 (MQ=255)
         cGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCaa                                                                                                                                        >  2:632844/1‑150 (MQ=255)
          gAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAaat                                                                                                                                      >  2:1118994/1‑151 (MQ=255)
           aaaCCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCTCTGAGCAATGCAGTGCCTGCAaata                                                                                                                                     <  2:1165947/151‑1 (MQ=255)
           aaaCCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAaat                                                                                                                                      >  2:518591/1‑150 (MQ=255)
             aCCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAaataat                                                                                                                                   <  3:455334/151‑1 (MQ=255)
               cAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGa                                                                                                                                 <  1:685124/151‑1 (MQ=255)
               cAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGa                                                                                                                                 <  1:237699/151‑1 (MQ=255)
               cAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGa                                                                                                                                 <  3:197122/151‑1 (MQ=255)
                aaCAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGAc                                                                                                                                <  1:936410/151‑1 (MQ=255)
                 aCAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACa                                                                                                                               <  2:1010523/151‑1 (MQ=255)
                 aCAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACa                                                                                                                               <  1:1043435/151‑1 (MQ=255)
                 aCAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACa                                                                                                                               <  1:851489/151‑1 (MQ=255)
                 aCAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCACTGAACGTTGCTCCAGACCAATCTGTCCTTTGGCTTCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAtataa                                                                                                                                    <  2:816567/146‑1 (MQ=255)
                     ccATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCa                                                                                                                           <  3:397198/151‑1 (MQ=255)
                      cATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCAt                                                                                                                          <  2:342672/151‑1 (MQ=255)
                      cATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCAt                                                                                                                          <  4:354588/151‑1 (MQ=255)
                        tCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATcc                                                                                                                        >  2:1186995/1‑151 (MQ=255)
                          aaGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATccc                                                                                                                       >  2:991894/1‑150 (MQ=255)
                           aGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCaa                                                                                                                     <  4:413597/151‑1 (MQ=255)
                            gATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCaa                                                                                                                     >  1:296627/1‑150 (MQ=255)
                             aTTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAAc                                                                                                                   <  2:1024320/151‑1 (MQ=255)
                               tGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACt                                                                                                                  <  2:1060857/150‑1 (MQ=255)
                                gCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACtt                                                                                                                 <  1:905263/150‑1 (MQ=255)
                                   ggAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTAt                                                                                                             <  1:1216140/151‑1 (MQ=255)
                                        aaCGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAt                                                                                                        >  1:722400/1‑151 (MQ=255)
                                         aCGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAtt                                                                                                       <  2:340797/151‑1 (MQ=255)
                                          cGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTAttt                                                                                                      <  4:179626/151‑1 (MQ=255)
                                           gACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTc                                                                                                     <  2:969643/151‑1 (MQ=255)
                                            aCAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTcc                                                                                                    >  2:556992/1‑151 (MQ=255)
                                                 cAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAg                                                                                                <  1:323070/150‑1 (MQ=255)
                                                 cAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGt                                                                                               <  3:224256/151‑1 (MQ=255)
                                                 cAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGt                                                                                               <  4:140024/151‑1 (MQ=255)
                                                 cAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGt                                                                                               <  1:421449/151‑1 (MQ=255)
                                                 cAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGt                                                                                               <  2:1035391/151‑1 (MQ=255)
                                                 cAACATCAGGCCAAATGCCGTCAGTACCAGTGAACGTGGCTCCAAACCAATCTGTCTTTTGGGTTCTAACAGCGCAGAATTACAGCCACTGAGCATTGCAGTGCCTGCAATTAATGACAACCATCCCAAACTTTTATTGTATTTcc                                                                                                    >  2:721313/1‑146 (MQ=255)
                                                  aaCATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGtc                                                                                              <  2:816881/151‑1 (MQ=255)
                                                  aaCATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGtc                                                                                              <  2:94369/151‑1 (MQ=255)
                                                    cATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGtctc                                                                                            >  1:1183281/1‑151 (MQ=255)
                                                     aTCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCa                                                                                           <  3:78315/151‑1 (MQ=255)
                                                       cAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACATCCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAtt                                                                                         <  4:215178/151‑1 (MQ=255)
                                                       cAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCAtt                                                                                         <  1:1118381/151‑1 (MQ=255)
                                                         ggCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTa                                                                                       <  2:415606/151‑1 (MQ=255)
                                                            cAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACg                                                                                    <  2:539578/151‑1 (MQ=255)
                                                            cAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACg                                                                                    >  1:297918/1‑151 (MQ=255)
                                                             aaaTGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGa                                                                                   <  2:1119956/151‑1 (MQ=255)
                                                              aaTGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGAc                                                                                  >  1:417402/1‑151 (MQ=255)
                                                                 gCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCt                                                                                <  1:179377/150‑1 (MQ=255)
                                                                 gCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCATGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTc                                                                               >  2:973541/1‑151 (MQ=255)
                                                                   cGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCaa                                                                             <  2:816695/151‑1 (MQ=255)
                                                                        gTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCa                                                                        <  1:799487/151‑1 (MQ=255)
                                                                            cAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACgg                                                                     <  2:195148/150‑1 (MQ=255)
                                                                              gTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAATGACCTCAATTCCACGGGAt                                                                  >  1:335315/1‑151 (MQ=255)
                                                                              gTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGAt                                                                  <  2:1279712/151‑1 (MQ=255)
                                                                               tGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATc                                                                 >  1:1131771/1‑151 (MQ=255)
                                                                                 aaCGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtg                                                               <  2:563137/151‑1 (MQ=255)
                                                                                   cGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggt                                                             <  1:536686/151‑1 (MQ=255)
                                                                                   agTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggt                                                             >  2:458438/2‑151 (MQ=255)
                                                                                       gCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCg                                                         >  4:324620/1‑151 (MQ=255)
                                                                                         tCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGtt                                                       >  1:730022/1‑151 (MQ=255)
                                                                                          ccAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGttt                                                      >  1:1189702/1‑151 (MQ=255)
                                                                                          ccAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGtt                                                       <  1:427828/150‑1 (MQ=255)
                                                                                            aGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCGGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTa                                                     >  2:745507/1‑150 (MQ=255)
                                                                                                   tCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAgtgtgt                                             >  2:179416/1‑151 (MQ=255)
                                                                                                      gTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTggg                                          <  3:417810/151‑1 (MQ=255)
                                                                                                      gTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTgg                                           <  2:376039/150‑1 (MQ=255)
                                                                                                      gTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTgg                                           <  2:653319/150‑1 (MQ=255)
                                                                                                       tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTTGTGGCGTTTAAAGTGTGTggg                                          <  2:440208/150‑1 (MQ=255)
                                                                                                       tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTggg                                          >  2:780078/1‑150 (MQ=255)
                                                                                                       tCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTgg                                           <  3:324616/149‑1 (MQ=255)
                                                                                                        ccTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGc                                         >  2:352125/1‑150 (MQ=255)
                                                                                                             gggATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCAtttt                                    <  1:1267363/150‑1 (MQ=255)
                                                                                                             gggATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTa                                   <  2:419669/151‑1 (MQ=255)
                                                                                                              ggATCTAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTAc                                  >  1:82082/1‑151 (MQ=255)
                                                                                                                   tAACAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGa                              >  1:1070530/1‑150 (MQ=255)
                                                                                                                    aaCAGCGCAGAATTGCAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAGAGTGTGTGGGCATTTTACGGGaa                             <  2:15212/150‑1 (MQ=255)
                                                                                                                      cAGCGCAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGt                          >  1:439478/1‑151 (MQ=255)
                                                                                                                         cgcAGAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTAc                       <  1:182323/151‑1 (MQ=255)
                                                                                                                             gAATTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTa                   <  3:67369/151‑1 (MQ=255)
                                                                                                                              aaTTACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTAc                  >  1:168896/1‑151 (MQ=255)
                                                                                                                                 tACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGTGCATTTTACGGGAAGGTTACATTACTg                <  3:60039/150‑1 (MQ=255)
                                                                                                                                 tACAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTg                <  3:24981/150‑1 (MQ=255)
                                                                                                                                   cAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTaa             <  2:915702/151‑1 (MQ=255)
                                                                                                                                   cAGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTa              >  2:612552/1‑150 (MQ=255)
                                                                                                                                    aGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTaaa            <  3:290155/151‑1 (MQ=255)
                                                                                                                                    aGCCACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTaa             >  2:403545/1‑150 (MQ=255)
                                                                                                                                      ccACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGTGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAAc           >  2:454206/1‑150 (MQ=255)
                                                                                                                                      ccACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACa          <  3:65718/151‑1 (MQ=255)
                                                                                                                                       cACTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACAt         >  1:616228/1‑151 (MQ=255)
                                                                                                                                        aCTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATg        >  2:563268/1‑151 (MQ=255)
                                                                                                                                         cTGAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATg        >  1:1278860/1‑150 (MQ=255)
                                                                                                                                           gAGCAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGAtt     <  2:296583/151‑1 (MQ=255)
                                                                                                                                              cAATGCAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTaaa  <  2:427991/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTTCCAGGCGAAACCAACAGCCATCAAGATTGCGGGAATAACGACAATCAACATCAGGCCAAATGCCGTCAGTATCAGTGAACGTTGCTCCAGACCAATCTGTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAA  >  NC_000913/451406‑451698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: