Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 460,036 C→T G383G (GGC→GGT)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913460,0360CT96.9% 344.8 / NA 99G383G (GGC→GGT) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/48);  minor base G (0/3);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 102 total aligned reads displayed.

CCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATG  >  NC_000913/459889‑460183
                                                                                                                                                   |                                                                                                                                                   
ccTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGggtggc                                                                                                                                                  >  3:118942/1‑151 (MQ=255)
 cTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGgtggcg                                                                                                                                                 >  1:900277/1‑151 (MQ=255)
   tGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGgtggcg                                                                                                                                                 <  1:577084/149‑1 (MQ=255)
   tGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGgcgtg                                                                                                                                               >  4:444684/1‑151 (MQ=255)
   tGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGgcgtg                                                                                                                                               >  2:330241/1‑151 (MQ=255)
    gAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGgcgtgc                                                                                                                                              <  1:291030/151‑1 (MQ=255)
    gAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGgcgtgc                                                                                                                                              <  1:68391/151‑1 (MQ=255)
    gAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGgcgtg                                                                                                                                               >  2:1128581/1‑150 (MQ=255)
     aGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGgcgtgcg                                                                                                                                             >  2:1123977/1‑151 (MQ=255)
       tATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGcgtgcgtg                                                                                                                                           >  4:18244/1‑151 (MQ=255)
        aTCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGcgtgcgtg                                                                                                                                           <  1:438665/150‑1 (MQ=255)
        aTCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGcgtgcgtg                                                                                                                                           <  1:633700/150‑1 (MQ=255)
         tCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGtgat                                                                                                                                         >  3:58271/1‑151 (MQ=255)
            tGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGtgatga                                                                                                                                       <  3:442138/150‑1 (MQ=255)
            tGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGtgatga                                                                                                                                       <  3:369423/150‑1 (MQ=255)
                gTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCgg                                                                                                                                  >  1:1256101/1‑151 (MQ=255)
                gTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCgg                                                                                                                                  >  4:342698/1‑151 (MQ=255)
                   cAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGaa                                                                                                                                <  4:149141/150‑1 (MQ=255)
                    aaaGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAAt                                                                                                                              >  3:354831/1‑151 (MQ=255)
                     aaGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATc                                                                                                                             >  3:340141/1‑151 (MQ=255)
                      tgCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATcc                                                                                                                            >  2:728730/2‑151 (MQ=255)
                        ccGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGt                                                                                                                          >  4:280405/1‑151 (MQ=255)
                        ccGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGt                                                                                                                          >  1:1230873/1‑151 (MQ=255)
                         tgtgtCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTg                                                                                                                         >  2:758562/2‑151 (MQ=255)
                          gtgtCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTgg                                                                                                                        <  2:1150327/151‑1 (MQ=255)
                           tgtCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTgg                                                                                                                        >  2:915136/1‑150 (MQ=255)
                             tCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTc                                                                                                                      >  2:1087917/1‑150 (MQ=255)
                                  aaaaTCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCAccgcc                                                                                                                <  1:477411/151‑1 (MQ=255)
                                    agtCAAGGGACCGATCGTCTGCCTGGTAGGGCCGCCGGGGGTAGGAAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGGGTAAATATGTCCGTAGGGCGCTGGGAGGCGTGCGTGATGAAGCGGAAATCCGAGGTCAccgcag                                                                                                               >  2:594413/3‑148 (MQ=255)
                                    aaTCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGGGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGt                                                                                                              <  1:1174282/151‑1 (MQ=255)
                                        aaGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTTTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCCCCGCCGTACt                                                                                                           <  2:333473/150‑1 (MQ=255)
                                            gACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACAt                                                                                                      >  4:92877/1‑151 (MQ=255)
                                              ccGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATc                                                                                                     <  2:113826/150‑1 (MQ=255)
                                                gATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGt                                                                                                  >  1:1208155/1‑151 (MQ=255)
                                                gATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGAAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGt                                                                                                  <  2:1001378/151‑1 (MQ=255)
                                                 aTCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGtt                                                                                                 >  2:1164277/1‑151 (MQ=255)
                                                     tctGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTAt                                                                                             >  1:571836/1‑151 (MQ=255)
                                                        gCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGcc                                                                                          <  4:89312/151‑1 (MQ=255)
                                                        gCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGcc                                                                                          >  3:44775/1‑151 (MQ=255)
                                                           tGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGGGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTAt                                                                                             <  4:276799/145‑1 (MQ=255)
                                                            gttggggCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAACCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGt                                                                                      <  3:9153/147‑1 (MQ=255)
                                                            ggTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCggg                                                                                       >  3:55210/1‑150 (MQ=255)
                                                               aGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTaa                                                                                    >  4:223160/1‑150 (MQ=255)
                                                               aGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTaa                                                                                    <  3:295074/150‑1 (MQ=255)
                                                                 ggCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACt                                                                                 >  1:471990/1‑151 (MQ=255)
                                                                  gccgccGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCGGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTg                                                                                <  1:386843/151‑1 (MQ=255)
                                                                   ccgccgGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGTGGGCGTGCGTGATGAAGCGGAAATCCGTGGTTACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTg                                                                                <  2:306712/150‑1 (MQ=255)
                                                                   ccgccgGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTg                                                                                >  3:10505/1‑150 (MQ=255)
                                                                        gggggTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATcc                                                                            >  1:15717/1‑149 (MQ=255)
                                                                        gggggTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCa                                                                           <  2:277876/150‑1 (MQ=255)
                                                                         ggggTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAACCCGGGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGa                                                                         <  2:65021/151‑1 (MQ=255)
                                                                         ggggTAGGTAAAACCTCTCTTGGTCAGTCCATGGCCAAAGCCACCGGGCGTAAATATGTCGGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGa                                                                         <  3:382215/151‑1 (MQ=255)
                                                                               ggtaAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATgg                                                                   <  1:962446/151‑1 (MQ=255)
                                                                               ggtaAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATgg                                                                   <  2:118162/151‑1 (MQ=255)
                                                                                  aaaaCCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGc                                                                  <  2:536696/149‑1 (MQ=255)
                                                                                  aaaaCCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGa                                                                <  3:69429/151‑1 (MQ=255)
                                                                                  aaaaCCTCGTTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATTCCCGTATGGCGCTGGGGGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGa                                                                <  2:286058/151‑1 (MQ=255)
                                                                                   aaaCCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGa                                                                >  3:217934/1‑150 (MQ=255)
                                                                                     aCCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGaa                                                               >  4:123129/1‑149 (MQ=255)
                                                                                      ccTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAg                                                             >  2:954930/1‑150 (MQ=255)
                                                                                      ccTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGt                                                            <  2:519486/151‑1 (MQ=255)
                                                                                        tctctTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTgg                                                          >  1:266233/1‑151 (MQ=255)
                                                                                         ctctTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTg                                                           <  1:1082953/149‑1 (MQ=255)
                                                                                             tGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGc                                                        <  3:101321/148‑1 (MQ=255)
                                                                                             tGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTg                                                     >  4:244178/1‑151 (MQ=255)
                                                                                             tGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTg                                                     >  2:350652/1‑151 (MQ=255)
                                                                                                 cAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGaaaa                                                 <  4:449495/151‑1 (MQ=255)
                                                                                                    tCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAAcc                                              >  1:11971/1‑151 (MQ=255)
                                                                                                     ccATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAAccc                                             >  3:103707/1‑151 (MQ=255)
                                                                                                      cATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCg                                            <  3:412814/151‑1 (MQ=255)
                                                                                                         tGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGTTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCt                                          <  3:218783/150‑1 (MQ=255)
                                                                                                          gCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                         <  2:70220/150‑1 (MQ=255)
                                                                                                          gCCAAAGCAACCGGGCGGAAATAGGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAGGCGGAAAGCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGt                                        <  2:121746/151‑1 (MQ=255)
                                                                                                            cAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTc                                      >  4:188658/1‑151 (MQ=255)
                                                                                                              aaGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCt                                    >  1:589491/1‑151 (MQ=255)
                                                                                                                 ccACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGGGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg                                   <  4:124723/149‑1 (MQ=255)
                                                                                                                  cACCGGTCGTAAATAGTGCCGTATGGGGCTGGGTGGCGTGGGTAATGACGCGGAAATCGGTGCTCGCCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATGCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc                                <  1:789639/151‑1 (MQ=255)
                                                                                                                  cACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc                                >  2:452835/1‑151 (MQ=255)
                                                                                                                  cACCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc                                <  1:1009706/151‑1 (MQ=255)
                                                                                                                   aCCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCg                               >  3:323081/1‑151 (MQ=255)
                                                                                                                   aCCGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGGTCGTGATGAAGCGGAAATCCGGGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGGAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCg                               >  4:52085/1‑151 (MQ=255)
                                                                                                                     cGGGCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                             >  2:896615/1‑151 (MQ=255)
                                                                                                                      ggggGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                             <  1:821446/150‑1 (MQ=255)
                                                                                                                      gggCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAACCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                             <  3:426455/150‑1 (MQ=255)
                                                                                                                       ggCGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                            >  1:834341/1‑150 (MQ=255)
                                                                                                                       ggCGTAAATATGCCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATga                           <  1:1256080/151‑1 (MQ=255)
                                                                                                                         cGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgaga                         <  2:15716/151‑1 (MQ=255)
                                                                                                                         cGTAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                            >  4:447287/1‑148 (MQ=255)
                                                                                                                           tAAATATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                            >  3:456180/1‑146 (MQ=255)
                                                                                                                            aaaTATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                            >  4:251685/1‑145 (MQ=255)
                                                                                                                            aaaTATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                            >  2:1146742/1‑145 (MQ=255)
                                                                                                                            aaaTATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATc                       <  1:1218580/150‑1 (MQ=255)
                                                                                                                            aaaTATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCg                      <  1:1131262/151‑1 (MQ=255)
                                                                                                                             aaTATGTCCGTATGGCGCTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCg                      >  2:1205556/1‑150 (MQ=255)
                                                                                                                             aaTATGTCCGTAGGGCGCTGGGTGGCGTGCGTGAAGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGa                     <  4:198647/151‑1 (MQ=255)
                                                                                                                                           gcgcTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTg       <  2:571725/151‑1 (MQ=255)
                                                                                                                                              cTGGGTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGa      <  3:352739/149‑1 (MQ=255)
                                                                                                                                                gggTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACa    <  1:125290/149‑1 (MQ=255)
                                                                                                                                                gggTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACAt   <  2:61072/150‑1 (MQ=255)
                                                                                                                                                gggTGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATg  <  4:43500/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTAAAACCTCTCTTGGTCAGTCCATTGCCAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATG  >  NC_000913/459889‑460183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑