Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,458	T→C	V24A (GTC→GCC)	yacL →	UPF0231 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,458	0	T	C	100.0%	369.0 / NA	100	V24A (GTC→GCC)	yacL	UPF0231 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/45);  total (55/45)

TTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGA  >  NC_000913/134310‑134604                                                                                                                                                     |                                                                                                                                                   ttCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCg                                                                                                                                                  >  1:547709/1‑151 (MQ=255)   ctcCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCggg                                                                                                                                                >  2:1583/1‑151 (MQ=255)     ccGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCggg                                                                                                                                                <  4:310640/149‑1 (MQ=255)        gTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTg                                                                                                                                           <  1:383967/151‑1 (MQ=255)         tAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTgg                                                                                                                                          >  1:196099/1‑151 (MQ=255)         tAAGGCGGCGATAATTACATCAGTGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATTGATTACGAATTTCTGCGCGATCTTACCGGAGTGGTAAAGGTGCGTATGTCCCTGGGGCATGAAGTGGCCGGGCACTgg                                                                                                                                          >  3:104564/1‑151 (MQ=255)          aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                         >  4:429363/1‑151 (MQ=255)          aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                         <  2:343970/151‑1 (MQ=255)          aaGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGt                                                                                                                                         >  2:195696/1‑151 (MQ=255)           aGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGtt                                                                                                                                        >  2:691290/1‑151 (MQ=255)           aGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGtt                                                                                                                                        >  3:60206/1‑151 (MQ=255)            ggCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGttt                                                                                                                                       >  3:214967/1‑151 (MQ=255)                gCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATg                                                                                                                                   <  1:1276381/151‑1 (MQ=255)                   aTAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGa                                                                                                                                  >  1:1237141/1‑149 (MQ=255)                     aaTTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAaga                                                                                                                               <  2:96399/150‑1 (MQ=255)                       ttACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAgg                                                                                                                             >  4:190101/1‑150 (MQ=255)                        tACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGt                                                                                                                            <  2:175599/150‑1 (MQ=255)                         aCATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTg                                                                                                                           >  4:179918/1‑150 (MQ=255)                          cATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaa                                                                                                                         <  4:153161/151‑1 (MQ=255)                           agcAATGGCGCAATGCGATTTCGGTGCATTGCAGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCAGGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaa                                                                                                                         <  3:343690/148‑1 (MQ=255)                            tCAATGGCGCAATGCGCTTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGGGGCCGGGCACTGGTTTAATGAAGAGGTgaa                                                                                                                         >  2:507702/1‑149 (MQ=255)                                tGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTgaaagaaa                                                                                                                    >  2:617551/1‑150 (MQ=255)                                 ggCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAAc                                                                                                                  <  1:691429/151‑1 (MQ=255)                                      aaTGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGc                                                                                                             >  3:195578/1‑151 (MQ=255)                                         gCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCct                                                                                                           <  1:11036/150‑1 (MQ=255)                                          cGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttg                                                                                                         >  1:107241/1‑151 (MQ=255)                                          cGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttg                                                                                                         <  4:354761/151‑1 (MQ=255)                                           gATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCcttgc                                                                                                        <  2:1003000/151‑1 (MQ=255)                                                 ggTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgatg                                                                                                  >  1:1065897/1‑151 (MQ=255)                                                  gTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgatga                                                                                                 <  2:139792/151‑1 (MQ=255)                                                   tGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTtgatga                                                                                                 >  2:265111/1‑150 (MQ=255)                                                    gCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAg                                                                                               <  2:966404/151‑1 (MQ=255)                                                     cATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGt                                                                                              >  1:129523/1‑151 (MQ=255)                                                     cATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGt                                                                                              <  3:337184/151‑1 (MQ=255)                                                       ttGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTgg                                                                                            >  1:220794/1‑151 (MQ=255)                                                        tGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTgg                                                                                            <  3:138687/150‑1 (MQ=255)                                                         gCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGa                                                                                           >  1:990854/1‑150 (MQ=255)                                                          ccGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAAc                                                                                         <  1:755384/151‑1 (MQ=255)                                                           cGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAAc                                                                                         >  1:445196/1‑150 (MQ=255)                                                            gggAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACa                                                                                        >  4:208567/1‑150 (MQ=255)                                                               aGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagca                                                                                    <  3:173188/151‑1 (MQ=255)                                                                gCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagcag                                                                                   <  1:971819/151‑1 (MQ=255)                                                                 cAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAagcagc                                                                                  >  2:380943/1‑151 (MQ=255)                                                                    agGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAgcgc                                                                                >  3:413291/1‑150 (MQ=255)                                                                     ggAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGcgcac                                                                              >  1:614937/1‑151 (MQ=255)                                                                          acacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACt                                                                         >  1:140093/1‑151 (MQ=255)                                                                           cacTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTg                                                                        >  4:293052/1‑151 (MQ=255)                                                                            acTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGa                                                                       <  4:259971/151‑1 (MQ=255)                                                                                 ggATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAgg                                                                   <  1:396717/150‑1 (MQ=255)                                                                                  gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGt                                                                  >  2:312604/1‑150 (MQ=255)                                                                                  gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTa                                                                 <  3:344621/151‑1 (MQ=255)                                                                                  gATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTa                                                                 <  1:1157781/151‑1 (MQ=255)                                                                                    ttACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGc                                                               >  1:584548/1‑151 (MQ=255)                                                                                      aCGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCg                                                              <  1:393174/150‑1 (MQ=255)                                                                                        gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                           >  4:97877/1‑151 (MQ=255)                                                                                        gAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAAc                                                           >  1:20299/1‑151 (MQ=255)                                                                                            ttCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTc                                                       >  3:23962/1‑151 (MQ=255)                                                                                              cTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCt                                                     >  2:664653/1‑151 (MQ=255)                                                                                                gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTcc                                                      <  4:207352/148‑1 (MQ=255)                                                                                                gcgcGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTg                                                    <  4:114750/150‑1 (MQ=255)                                                                                                  gcgATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGc                                                  >  2:880091/1‑150 (MQ=255)                                                                                                    gATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAAc                                               >  2:1243007/1‑151 (MQ=255)                                                                                                    gATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAAc                                               >  2:650200/1‑151 (MQ=255)                                                                                                    gATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAAc                                               >  2:500769/1‑151 (MQ=255)                                                                                                     atatTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACg                                              <  2:739451/151‑1 (MQ=255)                                                                                                           ccGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagg                                        >  1:527739/1‑151 (MQ=255)                                                                                                            cGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcaggg                                       >  3:209855/1‑151 (MQ=255)                                                                                                             ggAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGTGCCTGGCAACgggcaggg                                       >  4:56477/1‑150 (MQ=255)                                                                                                             ggAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACgggcagggc                                      <  1:1223767/151‑1 (MQ=255)                                                                                                                 tGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCAt                                    <  3:174453/149‑1 (MQ=255)                                                                                                                  ggTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGaa                                 <  2:598614/151‑1 (MQ=255)                                                                                                                  ggTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGa                                  <  1:377830/150‑1 (MQ=255)                                                                                                                     aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATac                              >  2:1098495/1‑151 (MQ=255)                                                                                                                     aaaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATac                              <  4:16019/151‑1 (MQ=255)                                                                                                                      aaGGTGCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATac                              >  2:519113/1‑150 (MQ=255)                                                                                                                           gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCt                                                     <  1:174965/122‑1 (MQ=255)                                                                                                                           gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCt                                                     >  2:174942/1‑122 (MQ=255)                                                                                                                           gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCtg                        >  1:1040924/1‑151 (MQ=255)                                                                                                                           gCGTATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCt                         <  1:1081971/150‑1 (MQ=255)                                                                                                                              tATGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgg                     >  2:560635/1‑151 (MQ=255)                                                                                                                               aTGTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtgga                    <  4:249439/151‑1 (MQ=255)                                                                                                                                 gTCCATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGtggatg                  >  1:1029647/1‑151 (MQ=255)                                                                                                                                    cATGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGAc               <  4:295694/151‑1 (MQ=255)                                                                                                                                     aTGGGGCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACg              >  1:671524/1‑151 (MQ=255)                                                                                                                                       ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGt            <  2:906246/151‑1 (MQ=255)                                                                                                                                       ggggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGt            <  1:849623/151‑1 (MQ=255)                                                                                                                                        gggCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTg           <  1:1583/151‑1 (MQ=255)                                                                                                                                          gCATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGaa         <  2:196071/151‑1 (MQ=255)                                                                                                                                           cATGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTGGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGa          >  3:208920/1‑149 (MQ=255)                                                                                                                                            aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGa          >  2:999806/1‑148 (MQ=255)                                                                                                                                            aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAaga       <  2:563039/151‑1 (MQ=255)                                                                                                                                            aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAaga       <  1:122849/151‑1 (MQ=255)                                                                                                                                            aTGAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAaga       <  2:172869/151‑1 (MQ=255)                                                                                                                                              gAAGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAagag      <  1:1235773/150‑1 (MQ=255)                                                                                                                                               aaGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAgg     >  1:195963/1‑150 (MQ=255)                                                                                                                                               aaGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGt    >  1:178270/1‑151 (MQ=255)                                                                                                                                                aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg   >  2:1053597/1‑151 (MQ=255)                                                                                                                                                aGTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTg   >  2:94922/1‑151 (MQ=255)                                                                                                                                                 gTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAgg     >  1:792729/1‑148 (MQ=255)                                                                                                                                                 gTGGCCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGa  <  3:401455/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGGCCTTGCTTGATGAAGTGGAACAAGCAGCGCACGCACTGAAAGGTAGCGAACGGTCCTGGCAACGGGCAGGGCATGAATACACCCTGTGGATGGACGGTGAAGAGGTGA  >  NC_000913/134310‑134604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑