Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 910,826 C→G G75A (GGC→GCC)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913910,8260CG99.0% 321.0 / NA 98G75A (GGC→GCC) poxBpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (49/48);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCG  >  NC_000913/910679‑910972
                                                                                                                                                   |                                                                                                                                                  
cATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGgcgc                                                                                                                                                 >  3:93117/1‑151 (MQ=255)
   tCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACg                                                                                                                                              >  2:888138/1‑151 (MQ=255)
     gcgGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGAt                                                                                                                                            <  1:611823/151‑1 (MQ=255)
      cgGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGAt                                                                                                                                            <  1:783569/150‑1 (MQ=255)
      cgGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATc                                                                                                                                           >  2:1265562/1‑151 (MQ=255)
       ggAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATcc                                                                                                                                          <  2:929342/151‑1 (MQ=255)
        gAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCg                                                                                                                                         <  1:414149/151‑1 (MQ=255)
          aTAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGgc                                                                                                                                       <  1:441522/151‑1 (MQ=255)
            aGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGgcgc                                                                                                                                     <  2:43155/151‑1 (MQ=255)
            aGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGgcgc                                                                                                                                     >  3:340676/1‑151 (MQ=255)
                 ttGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGAcc                                                                                                                                >  2:654965/1‑151 (MQ=255)
                 ttGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGAcc                                                                                                                                >  2:738814/1‑151 (MQ=255)
                  tgtgGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCg                                                                                                                               >  2:559576/1‑151 (MQ=255)
                  tgtgGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCg                                                                                                                               >  4:188104/1‑151 (MQ=255)
                   gtgggtggGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCg                                                                                                                               <  1:1016940/150‑1 (MQ=255)
                   gtgggtggGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGc                                                                                                                              <  2:501328/151‑1 (MQ=255)
                   gtgggtggGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGc                                                                                                                              >  2:1061226/1‑151 (MQ=255)
                     gggtgggtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGcc                                                                                                                             >  1:783368/1‑150 (MQ=255)
                     gggtgggtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCa                                                                                                                            <  1:139051/151‑1 (MQ=255)
                      ggtgggtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAg                                                                                                                           <  3:328622/151‑1 (MQ=255)
                           gtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTtctc                                                                                                                      >  4:174028/1‑151 (MQ=255)
                           gtTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTtctc                                                                                                                      >  2:804915/1‑151 (MQ=255)
                            tttCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGCGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTtctc                                                                                                                      >  1:1252876/1‑150 (MQ=255)
                              tCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCg                                                                                                                    <  4:241025/150‑1 (MQ=255)
                                cTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCt                                                                                                                  >  1:1196136/1‑150 (MQ=255)
                                  ggAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTa                                                                                                                 <  3:29098/149‑1 (MQ=255)
                                  ggAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTa                                                                                                                 >  2:43994/1‑149 (MQ=255)
                                          ccGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCtt                                                                                                       >  2:1245659/1‑151 (MQ=255)
                                           cGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTc                                                                                                      >  1:45896/1‑151 (MQ=255)
                                            gCTGCCAATTTCGGTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTc                                                                                                      >  2:1242245/1‑150 (MQ=255)
                                            gCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTc                                                                                                      >  1:442330/1‑150 (MQ=255)
                                                 cAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGcc                                                                                                >  4:171968/1‑151 (MQ=255)
                                                   aTTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGcc                                                                                                >  4:338645/1‑149 (MQ=255)
                                                      tCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCg                                                                                            <  2:801656/150‑1 (MQ=255)
                                                       cGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCg                                                                                            <  2:670543/149‑1 (MQ=255)
                                                        gCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCg                                                                                            <  3:29676/148‑1 (MQ=255)
                                                         cTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCa                                                                                         >  4:106825/1‑150 (MQ=255)
                                                           ggAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCaaa                                                                                       <  1:1229695/150‑1 (MQ=255)
                                                           ggAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAg                                                                                      <  1:739702/151‑1 (MQ=255)
                                                              gggAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgcc                                                                                   <  1:1170564/151‑1 (MQ=255)
                                                               ggAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccg                                                                                  <  2:928588/151‑1 (MQ=255)
                                                               ggAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccg                                                                                  <  1:992992/151‑1 (MQ=255)
                                                                gAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                                                                                 >  2:1108316/1‑151 (MQ=255)
                                                                gAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgc                                                                                 >  1:975274/1‑151 (MQ=255)
                                                                 aaTATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGgccgcc                                                                                <  2:850387/151‑1 (MQ=255)
                                                                    atGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCAct                                                                             >  1:91597/1‑151 (MQ=255)
                                                                    atGAGAGGCAATCGCCAGTACCGGAACGTGATTGTGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCAct                                                                             >  2:276533/1‑151 (MQ=255)
                                                                       aGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCActtct                                                                          >  3:344035/1‑151 (MQ=255)
                                                                        gCGGCAATCGCCAGTACCGGAACGTGATGGGGGTGGCAACCGAACGGGCCGTTGATTAAGGGCAGGTGGCCGGGGGCGCACGATCCGGCGCAGCCCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCAct                                                                             <  2:1088393/147‑1 (MQ=255)
                                                                         cGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCTACttcttc                                                                        >  1:716367/1‑151 (MQ=255)
                                                                         cGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACttcttc                                                                        >  2:68759/1‑151 (MQ=255)
                                                                          ggCAATCGCCAGTCCCGGACCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCCCGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACttcttc                                                                        <  2:199429/150‑1 (MQ=255)
                                                                          ggCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCAct                                                                             <  1:738054/145‑1 (MQ=255)
                                                                          ggCAAACGCCAGTACGGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGTTCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCActtctt                                                                         <  1:238628/149‑1 (MQ=255)
                                                                                 gCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCggg                                                                >  4:24279/1‑151 (MQ=255)
                                                                                  ccAGTCCCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGt                                                               <  2:95088/151‑1 (MQ=255)
                                                                                  ccAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGt                                                               <  2:663350/151‑1 (MQ=255)
                                                                                   cAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTg                                                              >  4:344026/1‑151 (MQ=255)
                                                                                      tACCGGAACGTGATTGCGGTGGCATTCGAACGGGCCGTTGATTAAGTGCGGGTGGCCGGGGGCGCCGGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGa                                                            <  2:936635/150‑1 (MQ=255)
                                                                                       aCCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCgggggg                                                             >  1:268356/1‑148 (MQ=255)
                                                                                       aCCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGAc                                                           >  1:205112/1‑150 (MQ=255)
                                                                                        ccGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGAc                                                           <  2:226507/149‑1 (MQ=255)
                                                                                        ccGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCACCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTgggga                                                          >  2:746058/1‑147 (MQ=255)
                                                                                        ccGGAACGTGAGTGCGGTGGCAACCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCCCGATCCGGCGCGGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGa                                                            <  2:1137707/148‑1 (MQ=255)
                                                                                           gAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCAAcc                                                       >  1:45624/1‑150 (MQ=255)
                                                                                            aaCGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGAATcccc                                                     >  1:148630/1‑149 (MQ=255)
                                                                                              cGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACt                                                    <  2:386975/150‑1 (MQ=255)
                                                                                                tGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATTCa                                                      >  2:1149938/1‑146 (MQ=255)
                                                                                                  aTTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGTTCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGAt                                                <  2:577250/150‑1 (MQ=255)
                                                                                                   ttGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTGGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGAt                                                <  2:587784/149‑1 (MQ=255)
                                                                                                    tGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGGATCCACTccacgg                                              >  2:306876/1‑145 (MQ=255)
                                                                                                     gCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGt                                             <  4:142694/150‑1 (MQ=255)
                                                                                                      cGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGGCATCCCCTCGATgg                                              >  1:686571/1‑148 (MQ=255)
                                                                                                      cGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGGCATCCACCCGCTtgtg                                            >  1:415578/1‑150 (MQ=255)
                                                                                                          ggCAATCGAACGGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCAt                                       <  1:156150/151‑1 (MQ=255)
                                                                                                          ggCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCAt                                       <  1:556319/151‑1 (MQ=255)
                                                                                                            cAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGGAGCCACCCGTGGGTGCCCCTg                                      >  2:789869/1‑150 (MQ=255)
                                                                                                             attCGAAAAGGCCGTTGATTAAGTGCAGGTTCCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCAt                                       <  2:1170750/146‑1 (MQ=255)
                                                                                                                 gAACGGGCCGTTGATTAAGGGCAGGTTCCCGGGGGCGCCCGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGAtt                                 <  2:504143/150‑1 (MQ=255)
                                                                                                                 gAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGAt                                  <  1:888313/149‑1 (MQ=255)
                                                                                                                  aaCAGGCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGGAATCCACCCCATGGGGcccccgcggtt                                 >  1:272860/1‑141 (MQ=255)
                                                                                                                   aCAGGCCGTTTATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAg                              <  2:1168866/151‑1 (MQ=255)
                                                                                                                      ggCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCCCCCGAAGGGGGCCa                                        >  2:850123/1‑138 (MQ=255)
                                                                                                                       gCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCACTCGCTGGTGGCCACGCGGTTAAGACt                           >  2:785416/1‑150 (MQ=255)
                                                                                                                       gCCGTTGATTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCACTCCATGGGGCCCATGCGATTTAGAc                            >  1:427262/1‑149 (MQ=255)
                                                                                                                              aTTAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTaa                   <  3:318149/151‑1 (MQ=255)
                                                                                                                                tAAGTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCACTCGATGGGGGCCATGCGATTTAAAATGTCAc                      >  2:936304/1‑146 (MQ=255)
                                                                                                                                 aaGGGCAGGTTGCCGGGGGCGCTCGATCCGCCCGAGCCCGCCAGTGCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAg                  <  2:318955/149‑1 (MQ=255)
                                                                                                                                   gTGCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCg              <  3:277309/151‑1 (MQ=255)
                                                                                                                                     gCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGAcc               <  2:999505/148‑1 (MQ=255)
                                                                                                                                     gCAGGTTGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGt             <  1:551179/150‑1 (MQ=255)
                                                                                                                                         gTTGCCGGGGGCCCACGATCCGGCGCAGCCCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCa          <  2:68647/149‑1 (MQ=255)
                                                                                                                                          ttGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCaga        <  1:124652/150‑1 (MQ=255)
                                                                                                                                           tGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGGCATCCCCCCGCTGGGGCCCCTGCGGTTTAAACTGTCACTAAAACCGtt            >  1:30133/1‑145 (MQ=255)
                                                                                                                                           tGCCGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGGGGACATCCACTCGATGGTGCCCCTGCGATTAAGACTGTCACTAAAACCGtt            >  4:181287/1‑145 (MQ=255)
                                                                                                                                             ccGGGGGCGCCCGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGt    <  1:262563/151‑1 (MQ=255)
                                                                                                                                             ccGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGGCATCCACTCGATGGTGCCCCTGCGGTTTAGACTGTCACTAAAACCGTTCagag       >  4:291511/1‑148 (MQ=255)
                                                                                                                                              cGGGGGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTc   <  4:139301/151‑1 (MQ=255)
                                                                                                                                               ggggGCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGAACGTTTAGAGAGTCg  >  1:197221/1‑151 (MQ=255)
                                                                                                                                                ggggCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGGGCCCCTGCGATTTAGACTGTCACTAAAACCGTTCAGAGAGGCg  >  4:452990/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATTCGCGGAATAGCTCTTGTGGGTGGGTTTCCTGGAAATAGCCGCTGCCAATTTCGCTGGAGGGAATATGAGCGGCAATCGCCAGTACCGGAACGTGATTGCGGTGGCAATCGAACAGGCCGTTGATTAAGTGCAGGTTGCCGGGGCCGCACGATCCGGCGCAGACCGCCAGTTCTCCGCTAAGTTGTGCTTCAGCGCCAGCGGCAAAGGCCGCCACTTCTTCGTGGCGGGTGGACATCCACTCGATGGTGCCCATGCGATTAAGACTGTCACTAAGACCGTTCAGAGAGTCG  >  NC_000913/910679‑910972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: