Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,042,089 T→C S708G (AGC→GGC)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,042,0890TC100.0% 376.3 / NA 98S708G (AGC→GGC) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/55);  total (43/55)

CTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACGCCCCACGA  >  NC_000913/1041941‑1042235
                                                                                                                                                    |                                                                                                                                                  
cTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCgg                                                                                                                                                  >  2:213210/1‑151 (MQ=255)
  gAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGgc                                                                                                                                                 <  4:138194/150‑1 (MQ=255)
      gCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGtt                                                                                                                                            <  4:152769/151‑1 (MQ=255)
      gCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGt                                                                                                                                             <  2:624806/150‑1 (MQ=255)
         ctGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGttt                                                                                                                                           <  1:770622/149‑1 (MQ=255)
         ctGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGttt                                                                                                                                           <  1:303778/149‑1 (MQ=255)
         ctGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTa                                                                                                                                          >  2:338741/1‑150 (MQ=255)
         ctGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTa                                                                                                                                          >  1:954571/1‑150 (MQ=255)
           gAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTaa                                                                                                                                         <  1:957449/149‑1 (MQ=255)
             ggCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATca                                                                                                                                      <  1:95429/150‑1 (MQ=255)
               cTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCacac                                                                                                                                   >  1:182795/1‑151 (MQ=255)
                  atgaCAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTACCTGTAAGCGGGGCCGGCGCGTTTAATCACACCa                                                                                                                                 >  3:3033/1‑150 (MQ=255)
                    gaCAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCAtt                                                                                                                               <  1:251267/150‑1 (MQ=255)
                    gaCAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCAtt                                                                                                                               <  3:102608/150‑1 (MQ=255)
                    gaCAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTg                                                                                                                              <  2:703931/151‑1 (MQ=255)
                    gaCAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTg                                                                                                                              <  2:713550/151‑1 (MQ=255)
                     aCAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTg                                                                                                                              <  1:813500/150‑1 (MQ=255)
                      cAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTg                                                                                                                              >  3:250902/1‑149 (MQ=255)
                      cAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTg                                                                                                                              >  4:171850/1‑149 (MQ=255)
                       aaaCGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAgg                                                                                                                           >  2:402987/1‑151 (MQ=255)
                       aaaCGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAgg                                                                                                                           <  2:364039/151‑1 (MQ=255)
                       aaaCGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAgg                                                                                                                           >  2:954324/1‑151 (MQ=255)
                        aaCGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAgg                                                                                                                           >  2:318894/1‑150 (MQ=255)
                          cGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGAt                                                                                                                         <  3:351453/150‑1 (MQ=255)
                           gaagaaCTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAg                                                                                                                       <  2:648613/151‑1 (MQ=255)
                              gaaCTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAgcgc                                                                                                                    >  2:850394/1‑151 (MQ=255)
                                aCTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCt                                                                                                                  >  2:1254815/1‑151 (MQ=255)
                                aCTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCt                                                                                                                  >  4:439659/1‑151 (MQ=255)
                                   gTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCttt                                                                                                                <  3:218407/150‑1 (MQ=255)
                                   gTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTa                                                                                                               <  4:387537/151‑1 (MQ=255)
                                      tAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAat                                                                                                             >  4:171006/1‑150 (MQ=255)
                                       aaTGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAatat                                                                                                           <  1:634703/151‑1 (MQ=255)
                                       aaTGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAatat                                                                                                           >  1:1154458/1‑151 (MQ=255)
                                        aTGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATAtt                                                                                                          >  3:5564/1‑151 (MQ=255)
                                        aTGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATAtt                                                                                                          >  1:136203/1‑151 (MQ=255)
                                          gCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTg                                                                                                         <  2:844253/150‑1 (MQ=255)
                                          gCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGa                                                                                                        <  4:113562/151‑1 (MQ=255)
                                           cGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGAc                                                                                                       <  1:905260/151‑1 (MQ=255)
                                               gACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACgcct                                                                                                   <  2:653387/151‑1 (MQ=255)
                                                     aaaaGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGt                                                                                             >  1:680239/1‑151 (MQ=255)
                                                          gCGTCCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTCATATTGACGCCTGCCTGTTcc                                                                                          >  1:327796/1‑149 (MQ=255)
                                                           cGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCTTTTAATCACCCCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGa                                                                                       <  2:1217890/151‑1 (MQ=255)
                                                            gTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGa                                                                                       <  1:1125361/150‑1 (MQ=255)
                                                               cACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGc                                                                                    <  4:198949/150‑1 (MQ=255)
                                                               cACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCt                                                                                   <  1:1162311/151‑1 (MQ=255)
                                                                aCGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTg                                                                                  <  1:981426/151‑1 (MQ=255)
                                                                 cgccgcATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGc                                                                                 <  1:893092/151‑1 (MQ=255)
                                                                 cgccgcATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGc                                                                                 <  1:371915/151‑1 (MQ=255)
                                                                    cgcATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCAtt                                                                              <  4:304450/151‑1 (MQ=255)
                                                                     gcATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTg                                                                             <  1:302202/151‑1 (MQ=255)
                                                                       aTCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACTCCTGCCTGTTCCAGACGCTGCATTGAc                                                                           <  1:1247090/151‑1 (MQ=255)
                                                                        tCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGAc                                                                           >  1:396446/1‑150 (MQ=255)
                                                                         ccGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACa                                                                          <  1:916255/150‑1 (MQ=255)
                                                                           ggCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAAc                                                                       >  4:153698/1‑151 (MQ=255)
                                                                             ccACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTc                                                                     >  1:438163/1‑151 (MQ=255)
                                                                             ccACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTc                                                                     >  1:833180/1‑151 (MQ=255)
                                                                             ccACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTc                                                                     >  2:1245288/1‑151 (MQ=255)
                                                                             ccACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTc                                                                     >  2:1204895/1‑151 (MQ=255)
                                                                               aCTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCAc                                                                   <  2:713145/151‑1 (MQ=255)
                                                                                 tttCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACct                                                                 >  1:1040132/1‑151 (MQ=255)
                                                                                  ttCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACctc                                                                >  1:323992/1‑151 (MQ=255)
                                                                                    cAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCtt                                                              <  1:633807/151‑1 (MQ=255)
                                                                                     aGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCtt                                                              <  1:831798/150‑1 (MQ=255)
                                                                                      gTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTg                                                            <  1:170801/151‑1 (MQ=255)
                                                                                        tttACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTgg                                                           <  3:27432/150‑1 (MQ=255)
                                                                                         ttACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGc                                                          <  4:307687/150‑1 (MQ=255)
                                                                                          tACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCg                                                         <  1:850566/150‑1 (MQ=255)
                                                                                             tctTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGgtgt                                                     >  4:451364/1‑151 (MQ=255)
                                                                                                ttCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTc                                                   >  3:85726/1‑150 (MQ=255)
                                                                                                 tctcGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCa                                                  >  2:1049670/1‑150 (MQ=255)
                                                                                                      gAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCaa                                            <  1:1133169/151‑1 (MQ=255)
                                                                                                        gTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCaaaa                                          <  2:367146/151‑1 (MQ=255)
                                                                                                        gTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCaaa                                           <  2:1261687/150‑1 (MQ=255)
                                                                                                        gTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCaaa                                           >  1:477779/1‑150 (MQ=255)
                                                                                                          aaCTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAAcg                                        >  4:344587/1‑151 (MQ=255)
                                                                                                           aCTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAAcgc                                       <  2:1271926/151‑1 (MQ=255)
                                                                                                           aCTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAAcgc                                       >  1:702940/1‑151 (MQ=255)
                                                                                                                aaCCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAAc                                  >  3:334138/1‑151 (MQ=255)
                                                                                                                aaCCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAAc                                  >  4:402083/1‑151 (MQ=255)
                                                                                                                    gTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACcagc                              <  1:340840/151‑1 (MQ=255)
                                                                                                                    gTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACcagc                              >  1:373739/1‑151 (MQ=255)
                                                                                                                    gTAATAGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACcagc                              >  1:543952/1‑151 (MQ=255)
                                                                                                                         aGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGc                          <  1:1193386/150‑1 (MQ=255)
                                                                                                                         aGTTATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCt                         <  2:182771/151‑1 (MQ=255)
                                                                                                                           ttATAGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTgg                       >  3:393438/1‑151 (MQ=255)
                                                                                                                             ataGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTg                     <  2:954381/151‑1 (MQ=255)
                                                                                                                              taGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGc                    >  4:342552/1‑151 (MQ=255)
                                                                                                                               aGCCGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGcc                   >  4:105129/1‑151 (MQ=255)
                                                                                                                                  cGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAAc                >  1:998018/1‑151 (MQ=255)
                                                                                                                                  cGTAACTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAAc                <  1:1165210/151‑1 (MQ=255)
                                                                                                                                     aaCTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAg              <  2:1034072/150‑1 (MQ=255)
                                                                                                                                     aaCTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAg              <  2:1187215/150‑1 (MQ=255)
                                                                                                                                      aCTGTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAAAAGa             >  4:398575/1‑150 (MQ=255)
                                                                                                                                         gTAAGCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACg          <  1:1068207/150‑1 (MQ=255)
                                                                                                                                             gCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACGCCCCa     <  3:388214/151‑1 (MQ=255)
                                                                                                                                             gCGGTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACGCCCCa     <  3:296988/151‑1 (MQ=255)
                                                                                                                                               ggTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACGCCCCACg   >  1:92743/1‑151 (MQ=255)
                                                                                                                                                gTGCCGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACGCCCCACGa  <  3:345446/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGTGCTGGCGCGTTTAATCACACCATTGAGGATAGCGCCTTTAATATTGACGCCTGCCTGTTCCAGACGCTGCATTGACAAACTCACCTCTTTGGCGGTGTTCAAGCCAAAACGCGCAACCAGCAGGCTGGTGCCAACAGAACGCCCCACGA  >  NC_000913/1041941‑1042235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: