Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,140,553 A→G H174R (CAC→CGC)  flgL → flagellar hook‑filament junction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,140,5530AG100.0% 418.1 / NA 114H174R (CAC→CGC) flgLflagellar hook‑filament junction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (72/42);  total (72/42)

Only 100 of 114 total aligned reads displayed.

AACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGA  >  NC_000913/1140408‑1140700
                                                                                                                                                 |                                                                                                                                                   
aaCACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACgg                                                                                                                                                <  2:214256/151‑1 (MQ=255)
  caccacTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGt                                                                                                                                              <  1:405963/151‑1 (MQ=255)
     cacTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGAc                                                                                                                                           >  1:773366/1‑151 (MQ=255)
     cacTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGGGa                                                                                                                                            >  1:1158651/1‑150 (MQ=255)
        tGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACaa                                                                                                                                         >  4:54806/1‑150 (MQ=255)
        tGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACaa                                                                                                                                         >  2:205289/1‑150 (MQ=255)
           cGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAAtt                                                                                                                                     <  2:647610/151‑1 (MQ=255)
             gTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAAtttt                                                                                                                                   >  1:558498/1‑151 (MQ=255)
              tAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTc                                                                                                                                  >  1:539553/1‑151 (MQ=255)
               aaCGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCg                                                                                                                                 <  4:79477/151‑1 (MQ=255)
               aaCGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCg                                                                                                                                 >  2:442410/1‑151 (MQ=255)
               aaCGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCg                                                                                                                                 >  1:204210/1‑151 (MQ=255)
                 cGGGCGCTACATTCTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGAc                                                                                                                               >  2:826930/1‑151 (MQ=255)
                     cgcTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGt                                                                                                                            >  4:371017/1‑150 (MQ=255)
                     cgcTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTa                                                                                                                           <  4:74421/151‑1 (MQ=255)
                       cTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTAtt                                                                                                                         >  1:1177212/1‑151 (MQ=255)
                        tACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTAtt                                                                                                                         >  1:104350/1‑150 (MQ=255)
                         aCATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTa                                                                                                                        <  4:290356/150‑1 (MQ=255)
                           aTTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTAcc                                                                                                                      >  4:167928/1‑150 (MQ=255)
                             ttttGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAg                                                                                                                    <  1:277703/150‑1 (MQ=255)
                              tttGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGc                                                                                                                   <  2:272584/150‑1 (MQ=255)
                               ttGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCa                                                                                                                  >  3:289708/1‑150 (MQ=255)
                                 gCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAAcg                                                                                                               >  3:262322/1‑151 (MQ=255)
                                 gCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAAc                                                                                                                <  1:334268/150‑1 (MQ=255)
                                  ccGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAAcgc                                                                                                              >  3:303075/1‑151 (MQ=255)
                                   cGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAAcgcg                                                                                                             >  2:862485/1‑151 (MQ=255)
                                      ttATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGt                                                                                                           >  2:291583/1‑150 (MQ=255)
                                       tataAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGt                                                                                                           >  1:433402/1‑149 (MQ=255)
                                       tataAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGt                                                                                                           >  1:789761/1‑149 (MQ=255)
                                         taAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCg                                                                                                       >  3:125871/1‑151 (MQ=255)
                                          aaaaCAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCgg                                                                                                      <  1:93419/151‑1 (MQ=255)
                                           aaaCAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGa                                                                                                     <  1:980514/151‑1 (MQ=255)
                                            aaCAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGaa                                                                                                    <  3:286048/151‑1 (MQ=255)
                                               agagACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAAcc                                                                                                  >  3:222657/1‑150 (MQ=255)
                                               agagACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCa                                                                                                 <  2:411764/151‑1 (MQ=255)
                                                gagaCTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAAcc                                                                                                  >  1:1189599/1‑149 (MQ=255)
                                                 agaCTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGa                                                                                               <  4:167772/151‑1 (MQ=255)
                                                     tGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGt                                                                                           >  4:384614/1‑151 (MQ=255)
                                                        gcCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAgc                                                                                        >  4:203579/1‑151 (MQ=255)
                                                         ccGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAgcg                                                                                       >  2:262032/1‑151 (MQ=255)
                                                         ccGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAgcg                                                                                       <  2:999301/151‑1 (MQ=255)
                                                          cGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAgcgc                                                                                      >  1:907427/1‑151 (MQ=255)
                                                          cGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAgcgc                                                                                      >  2:280443/1‑151 (MQ=255)
                                                             ttAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCtt                                                                                    <  2:105550/150‑1 (MQ=255)
                                                              tAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCt                                                                                  >  4:186968/1‑151 (MQ=255)
                                                                gCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGa                                                                                <  2:1042580/151‑1 (MQ=255)
                                                                gCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGa                                                                                <  1:401521/151‑1 (MQ=255)
                                                                  gAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCATTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGaaa                                                                              <  1:605722/151‑1 (MQ=255)
                                                                     gagaAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAAcc                                                                            >  1:506955/1‑150 (MQ=255)
                                                                      agaAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAAACAGACGGTAgc                                                                                        <  1:1165571/137‑1 (MQ=255)
                                                                      agaAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAAACAGACGGTAgc                                                                                        >  2:1165360/1‑137 (MQ=255)
                                                                       gaAAGGGAAGTACGTCTGTGGAGCATAAAGTATTAATCAACAGGTCGATGCGTCGCGTTCGATGGTGATAGGGCGCGCGGGTGACAAAATTTTCGACAGTATTACCAGCACCGCGGTAGCGGAATCAGACAGTAGCGCTTCTGAAGCCa                                                                           <  2:69054/149‑1 (MQ=255)
                                                                        aaaGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATc                                                                        >  1:8716/1‑151 (MQ=255)
                                                                           gggAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCttt                                                                     <  3:43995/151‑1 (MQ=255)
                                                                             gAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCttttt                                                                   <  2:528797/151‑1 (MQ=255)
                                                                             gAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCttttt                                                                   <  4:40895/151‑1 (MQ=255)
                                                                             gAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGACGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCttttt                                                                   >  2:86494/1‑151 (MQ=255)
                                                                              aaaTACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGAGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTg                                                                  >  2:578008/1‑151 (MQ=255)
                                                                               aaTACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGc                                                                 >  2:730783/1‑151 (MQ=255)
                                                                                    gTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATga                                                            <  2:550739/151‑2 (MQ=255)
                                                                                     tCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCt                                                           >  1:1111585/1‑151 (MQ=255)
                                                                                     tCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCt                                                           >  2:537087/1‑151 (MQ=255)
                                                                                        gTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTgg                                                         >  2:579492/1‑150 (MQ=255)
                                                                                        gTGGAGCAGAAAGTATTAAACAACAAGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTgg                                                         >  2:788908/1‑150 (MQ=255)
                                                                                          ggAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTCTTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATa                                                      <  4:291657/151‑1 (MQ=255)
                                                                                          ggAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATa                                                      <  1:222888/151‑1 (MQ=255)
                                                                                           gAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAg                                                     <  4:338768/151‑1 (MQ=255)
                                                                                              cAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGc                                                  >  2:974734/1‑151 (MQ=255)
                                                                                              cAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGc                                                  <  2:539453/151‑1 (MQ=255)
                                                                                                gAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGcc                                                 >  4:297853/1‑150 (MQ=255)
                                                                                                 aaaGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCAt                                               >  2:1067071/1‑151 (MQ=255)
                                                                                                      aTTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAg                                          <  4:13118/151‑1 (MQ=255)
                                                                                                        tAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGcc                                        >  2:883979/1‑151 (MQ=255)
                                                                                                         aaaCAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGccc                                       >  4:383532/1‑151 (MQ=255)
                                                                                                         aaaCAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGccc                                       >  2:1186845/1‑151 (MQ=255)
                                                                                                         aaaCAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGccc                                       <  3:164220/151‑1 (MQ=255)
                                                                                                          aacaacAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCt                                      >  2:381041/1‑151 (MQ=255)
                                                                                                          aacaacAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCt                                      <  2:587740/151‑1 (MQ=255)
                                                                                                           acaacaGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTg                                     <  2:942419/151‑1 (MQ=255)
                                                                                                             aacaGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGa                                    >  3:413885/1‑150 (MQ=255)
                                                                                                             aacaGGTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGa                                    >  1:260113/1‑150 (MQ=255)
                                                                                                                 ggTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACg                               >  2:463067/1‑151 (MQ=255)
                                                                                                                 ggTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACg                               <  2:837435/151‑1 (MQ=255)
                                                                                                                 ggTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACg                               >  3:139447/1‑151 (MQ=255)
                                                                                                                  gTCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGc                              <  4:232586/151‑1 (MQ=255)
                                                                                                                   tCGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGcc                             >  3:3345/1‑151 (MQ=255)
                                                                                                                    cGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGTCCTGAAAACGCCg                            >  3:83978/1‑151 (MQ=255)
                                                                                                                    cGATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGcc                             <  2:596719/150‑1 (MQ=255)
                                                                                                                     gATGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCgg                           >  1:516033/1‑151 (MQ=255)
                                                                                                                      aTGCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGt                          >  2:1269978/1‑151 (MQ=255)
                                                                                                                        gCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTcg                        >  3:327275/1‑151 (MQ=255)
                                                                                                                        gCTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTc                         <  1:100150/150‑1 (MQ=255)
                                                                                                                         cTTCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGAAGCGCTTCTGAAACCAATCTTTTTGCCATGTTGGATAGTGCCATCGCAGCCCTGAAAACGCCgg                           >  2:1031610/1‑147 (MQ=255)
                                                                                                                          ttCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGACACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTcgc                       >  2:791624/1‑150 (MQ=255)
                                                                                                                          ttCGCGTTCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTcgcg                      >  3:441919/1‑151 (MQ=255)
                                                                                                                                ttCGATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGc                >  1:1006930/1‑151 (MQ=255)
                                                                                                                                   gATGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGAAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCg               >  1:1083369/1‑149 (MQ=255)
                                                                                                                                    aTGGTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGa              >  2:615964/1‑149 (MQ=255)
                                                                                                                                      ggTGATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCg          >  1:349344/1‑151 (MQ=255)
                                                                                                                                         gATAGGGCGCACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGAt       <  2:920472/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AACACCACTGACGGTAACGGGCGCTACATTTTTGCCGGTTATAAAACAGAGACTGCGCCGTTTAGCGAAGAGAAAGGGAAATACGTCGGTGGAGCAGAAAGTATTAAACAACAGGTCGATGCTTCGCGTTCGATGGTGATAGGGCACACGGGTGACAAAATTTTCGACAGTATTACCAGCAACGCGGTAGCGGAACCAGACGGTAGCGCTTCTGAAACCAATCTTTTTGCCATGCTGGATAGTGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGA  >  NC_000913/1140408‑1140700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: