Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,242,307 C→T L258L (CTG→CTA ldcA ← murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,242,3070CT99.0% 416.0 / NA 105L258L (CTG→CTAldcAmurein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (51/52);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTT  >  NC_000913/1242161‑1242452
                                                                                                                                                  |                                                                                                                                                 
    tttACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCggg                                                                                                                                            >  1:1126481/1‑150 (MQ=255)
       aCATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAAc                                                                                                                                         <  2:949224/150‑1 (MQ=255)
        cATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGc                                                                                                                                       >  1:1122683/1‑151 (MQ=255)
        cATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGc                                                                                                                                       >  1:578818/1‑151 (MQ=255)
         aTTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGc                                                                                                                                       >  4:346993/1‑150 (MQ=255)
         aTTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCa                                                                                                                                      <  3:175839/151‑1 (MQ=255)
              aaGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACAAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAAc                                                                                                                                 <  3:186271/151‑1 (MQ=255)
               aGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAAcg                                                                                                                                <  1:1018069/151‑1 (MQ=255)
                gAACAAGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAAcgc                                                                                                                               <  1:429892/151‑1 (MQ=255)
                 aaCAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAAcgcg                                                                                                                              <  2:75954/151‑1 (MQ=255)
                 aaCAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAAcgcg                                                                                                                              <  2:144691/151‑1 (MQ=255)
                 aaCAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGAAACGCCAAAAcgcg                                                                                                                              >  3:150461/1‑151 (MQ=255)
                  aCAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGAGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAAAGCAAAAAcgcg                                                                                                                              >  1:965559/1‑150 (MQ=255)
                   cAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTa                                                                                                                            <  2:1009020/151‑1 (MQ=255)
                    aGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTac                                                                                                                           <  1:808114/151‑1 (MQ=255)
                      gATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTacac                                                                                                                         >  4:163083/1‑151 (MQ=255)
                      gATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTaca                                                                                                                          >  1:720884/1‑150 (MQ=255)
                       aTGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTacac                                                                                                                         >  1:1096354/1‑150 (MQ=255)
                         gACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGa                                                                                                                      <  4:251279/151‑1 (MQ=255)
                               tAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTcc                                                                                                                  >  2:918949/1‑149 (MQ=255)
                                aaTCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAgg                                                                                                               <  4:21017/151‑1 (MQ=255)
                                   cGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGtt                                                                                                             <  2:178019/150‑1 (MQ=255)
                                   cGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGtt                                                                                                             >  1:1001461/1‑150 (MQ=255)
                                   cGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTg                                                                                                            <  2:1035792/151‑1 (MQ=255)
                                      cAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTa                                                                                                          <  2:171361/150‑1 (MQ=255)
                                       aaCTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAAc                                                                                                        >  1:300334/1‑151 (MQ=255)
                                        aCTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAAcc                                                                                                       >  3:14552/1‑151 (MQ=255)
                                        aCTGAGTGCCTTCCCGCGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACCGACTCCAGGGTGTTAcc                                                                                                       >  4:56737/1‑151 (MQ=255)
                                         cTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAAcc                                                                                                       >  1:466270/1‑150 (MQ=255)
                                            aGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCg                                                                                                   <  1:829484/151‑1 (MQ=255)
                                            aGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGGTGTAACCTGCg                                                                                                   >  2:356372/1‑151 (MQ=255)
                                             gTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGt                                                                                                  <  2:151150/151‑1 (MQ=255)
                                               gCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTc                                                                                                 >  1:1261712/1‑150 (MQ=255)
                                               gCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCa                                                                                                <  2:831297/151‑1 (MQ=255)
                                                ccTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCa                                                                                                >  4:175990/1‑150 (MQ=255)
                                                ccTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCAt                                                                                               <  2:837260/151‑1 (MQ=255)
                                                   tCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATaa                                                                                             >  3:30384/1‑150 (MQ=255)
                                                   tCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATa                                                                                              <  2:452713/149‑1 (MQ=255)
                                                    cccGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAAt                                                                                            >  1:258524/1‑150 (MQ=255)
                                                    cccGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATc                                                                                           <  1:947207/151‑1 (MQ=255)
                                                    cccGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATc                                                                                           <  1:852038/151‑1 (MQ=255)
                                                     ccGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCa                                                                                          <  1:914465/151‑1 (MQ=255)
                                                      cGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCAt                                                                                         <  1:162972/151‑1 (MQ=255)
                                                        ggTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTg                                                                                       <  1:283499/151‑1 (MQ=255)
                                                          tgtTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTggg                                                                                     >  2:1026052/1‑151 (MQ=255)
                                                           gtTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGTAGGCAAAAAAGCGGACACTGAATCCAGGGTGTAACCCACGTCATAATCATTgg                                                                                      >  2:882774/1‑149 (MQ=255)
                                                           gtTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGc                                                                                    <  4:48238/151‑1 (MQ=255)
                                                                tCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGtgc                                                                                <  1:1250671/150‑1 (MQ=255)
                                                                tCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGtgc                                                                                <  2:320253/150‑1 (MQ=255)
                                                                 cAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGtgctg                                                                              <  1:283015/151‑1 (MQ=255)
                                                                  aGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGtgctgc                                                                             >  1:626791/1‑151 (MQ=255)
                                                                  aGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGtgctgc                                                                             >  1:1022221/1‑151 (MQ=255)
                                                                   gAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGcc                                                                            >  1:315879/1‑151 (MQ=255)
                                                                   gAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGcc                                                                            >  1:1210590/1‑151 (MQ=255)
                                                                      tGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACCCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGc                                                                          >  2:241066/1‑150 (MQ=255)
                                                                       ggCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCt                                                                         >  4:85108/1‑150 (MQ=255)
                                                                         cATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTaa                                                                       <  1:865341/150‑1 (MQ=255)
                                                                          aTGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGGTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTaa                                                                       >  3:59177/1‑149 (MQ=255)
                                                                            gtgCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGc                                                                    <  1:1108985/150‑1 (MQ=255)
                                                                               cgcCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTAcc                                                                >  2:868291/1‑151 (MQ=255)
                                                                                 cccAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCga                                                              <  1:134832/151‑1 (MQ=255)
                                                                                  ccAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCgag                                                             <  2:1074159/151‑1 (MQ=255)
                                                                                  ccAACGGCAGCGTAACAGTGCGTTGGTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCgag                                                             <  2:295972/151‑1 (MQ=255)
                                                                                   cAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAAACTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTAcc                                                                >  1:1153474/1‑147 (MQ=255)
                                                                                         cAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATc                                                      <  1:1241721/151‑1 (MQ=255)
                                                                                            cGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGcc                                                   <  2:361310/151‑1 (MQ=255)
                                                                                            cGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGcc                                                   <  1:236495/151‑1 (MQ=255)
                                                                                               aaCCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGCTAGGCGGGAACGCAAAAACACGTAAACCGACTCCAGGATGTAAACTGCGTCATAATCATTGGGCGTGCTGTCGCTAAAGCTACCGAGAATAATCGCCtt                                                 >  2:383164/1‑150 (MQ=255)
                                                                                               aaCCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCtt                                                 <  2:414740/150‑1 (MQ=255)
                                                                                               aaCCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCtt                                                 <  1:44674/150‑1 (MQ=255)
                                                                                               aaCCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCtt                                                 <  4:235872/150‑1 (MQ=255)
                                                                                                   gTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGAc                                            <  4:274183/151‑1 (MQ=255)
                                                                                                    tGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACg                                           >  4:333254/1‑151 (MQ=255)
                                                                                                      cGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGAGAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTGGTAACCTTCGGCATAATCATTGGGCGAGCTGCCGCTAAAGCTAAAGAGAATAAGCGACTTCTg                                              >  2:286391/1‑146 (MQ=255)
                                                                                                         tgttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGaataa                                                        >  4:43827/1‑133 (MQ=255)
                                                                                                         tgttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGaataa                                                        <  3:43827/133‑1 (MQ=255)
                                                                                                            tCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCaaa                                    >  1:254328/1‑150 (MQ=255)
                                                                                                             cATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAAt                                  >  1:246970/1‑151 (MQ=255)
                                                                                                                   cAAAATCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGc                            <  4:168045/151‑1 (MQ=255)
                                                                                                                    aaaaTCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGc                            >  4:800/1‑150 (MQ=255)
                                                                                                                    aaaaTCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGc                            >  1:1269973/1‑150 (MQ=255)
                                                                                                                     aaaTCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCAt                          >  2:822695/1‑151 (MQ=255)
                                                                                                                     aaaTCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGCGGCAAAATTCCCGCAt                          >  2:844306/1‑151 (MQ=255)
                                                                                                                       aTCGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCAt                          >  2:989267/1‑149 (MQ=255)
                                                                                                                         cGAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATa                      <  3:208627/151‑1 (MQ=255)
                                                                                                                          gAGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGAt                       >  1:1239024/1‑149 (MQ=255)
                                                                                                                           aGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGAt                       >  4:366122/1‑148 (MQ=255)
                                                                                                                           aGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGAt                       >  4:459342/1‑148 (MQ=255)
                                                                                                                           aGGCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATaga                    <  4:340196/151‑1 (MQ=255)
                                                                                                                             gCCGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGc                  >  1:496207/1‑151 (MQ=255)
                                                                                                                               cGGTAGTGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTCCGTCATAATCATTGCGCGTGCTGCCGCTAAAGCCACCGAGGATAATCGCCTTTTGACGTGGCAATATTCCCGCATGATagagacg                >  2:158574/1‑148 (MQ=255)
                                                                                                                               cGGTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTg                >  3:417324/1‑151 (MQ=255)
                                                                                                                                ggTAATGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGc               <  4:417330/151‑1 (MQ=255)
                                                                                                                                    aTGAGTAGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACa           <  3:57547/151‑1 (MQ=255)
                                                                                                                                     tGAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAAc            >  2:483027/1‑149 (MQ=255)
                                                                                                                                      gAGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGc         <  2:764659/151‑1 (MQ=255)
                                                                                                                                       aGTGGAATCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCa        <  2:1126913/151‑1 (MQ=255)
                                                                                                                                        gTGGAATCGATAGGCGGGAACCCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGc         >  2:201138/1‑149 (MQ=255)
                                                                                                                                             aTCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGtt  <  2:606236/151‑1 (MQ=255)
                                                                                                                                             aTCGATAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGtt  <  1:498474/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTT  >  NC_000913/1242161‑1242452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: