Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,297,386 A→G C246R (TGT→CGT)  adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,297,3860AG100.0% 459.9 / NA 118C246R (TGT→CGT) adhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/67);  total (51/67)

Only 100 of 118 total aligned reads displayed.

CCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATG  >  NC_000913/1297239‑1297531
                                                                                                                                                   |                                                                                                                                                 
ccGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGa                                                                                                                                                 <  2:591832/150‑1 (MQ=255)
 cgcgTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGAtt                                                                                                                                               <  2:1154044/151‑1 (MQ=255)
  gcgTTCAGCGCCCCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGGAACAGATGGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGAtt                                                                                                                                               <  1:586652/150‑1 (MQ=255)
   cgTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTAc                                                                                                                                             <  4:117966/151‑1 (MQ=255)
    gTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACg                                                                                                                                            >  1:627513/1‑151 (MQ=255)
     ttCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACg                                                                                                                                            <  3:94733/150‑1 (MQ=255)
     ttCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGc                                                                                                                                           >  2:544272/1‑151 (MQ=255)
     ttCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGc                                                                                                                                           >  3:286972/1‑151 (MQ=255)
          cgcACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTg                                                                                                                                      <  3:298341/151‑1 (MQ=255)
            cACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTc                                                                                                                                    <  1:454595/151‑1 (MQ=255)
            cACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTc                                                                                                                                    >  1:588944/1‑151 (MQ=255)
              ccGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTTCGCCGTTGTCGa                                                                                                                                  <  2:676251/151‑1 (MQ=255)
              ccGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGa                                                                                                                                  <  4:402936/151‑1 (MQ=255)
                   tttCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGtt                                                                                                                             >  4:12629/1‑151 (MQ=255)
                   tttCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGt                                                                                                                              <  2:891310/150‑1 (MQ=255)
                    ttCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGttt                                                                                                                            >  1:725619/1‑151 (MQ=255)
                     tCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGtttt                                                                                                                           >  2:8253/1‑151 (MQ=255)
                      cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGtttt                                                                                                                           <  4:247646/150‑1 (MQ=255)
                      cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGtttt                                                                                                                           <  1:388613/150‑1 (MQ=255)
                      cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGtttt                                                                                                                           <  4:315136/150‑1 (MQ=255)
                      cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTg                                                                                                                          <  4:387749/151‑1 (MQ=255)
                        ggATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTgg                                                                                                                         >  2:1234985/1‑150 (MQ=255)
                          aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGAc                                                                                                                       >  4:285111/1‑150 (MQ=255)
                            aaCATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACAt                                                                                                                     <  2:1053874/150‑1 (MQ=255)
                            aaCATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATc                                                                                                                    <  1:142827/151‑1 (MQ=255)
                               aTCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGt                                                                                                                 >  2:904700/1‑151 (MQ=255)
                               aTCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGt                                                                                                                 <  3:386273/151‑1 (MQ=255)
                                 ccTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTa                                                                                                                <  4:290041/150‑1 (MQ=255)
                                    gAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTAc                                                                                                               >  2:818669/1‑148 (MQ=255)
                                     aaCAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGAt                                                                                                           <  1:229597/151‑1 (MQ=255)
                                      aCAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATg                                                                                                          <  1:1043776/151‑1 (MQ=255)
                                       cAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGc                                                                                                         <  2:730989/151‑1 (MQ=255)
                                        aGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCa                                                                                                        <  2:7842/151‑1 (MQ=255)
                                         gCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCaa                                                                                                       <  1:75748/151‑1 (MQ=255)
                                          cTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAAc                                                                                                      >  2:1237319/1‑151 (MQ=255)
                                            ttCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACt                                                                                                     <  2:714986/150‑1 (MQ=255)
                                            ttCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTg                                                                                                    >  2:948055/1‑151 (MQ=255)
                                              cAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGc                                                                                                   <  1:593773/150‑1 (MQ=255)
                                               aGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCAc                                                                                                 >  1:1200718/1‑151 (MQ=255)
                                                gCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACg                                                                                                >  2:272328/1‑151 (MQ=255)
                                                 ctctTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACg                                                                                                <  2:1062922/150‑1 (MQ=255)
                                                   ctTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGttt                                                                                             >  2:717847/1‑151 (MQ=255)
                                                   ctTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGt                                                                                               <  3:65652/149‑1 (MQ=255)
                                                   ctTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGt                                                                                               <  1:719365/149‑1 (MQ=255)
                                                     ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGtt                                                                                              <  2:103766/148‑1 (MQ=255)
                                                     ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGtt                                                                                              <  2:1005799/148‑1 (MQ=255)
                                                     ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTg                                                                                            <  2:821638/150‑1 (MQ=255)
                                                         ccTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATc                                                                                       >  4:52768/1‑151 (MQ=255)
                                                            gCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATca                                                                                      <  2:933205/149‑1 (MQ=255)
                                                             cAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCTCGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATcagca                                                                                   <  1:1200547/151‑1 (MQ=255)
                                                             cAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATcagca                                                                                   <  1:396901/151‑1 (MQ=255)
                                                             aaaCAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATcagc                                                                                    >  3:339147/2‑150 (MQ=255)
                                                              aaCAGATAGCCGCCGTGTGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATcagc                                                                                    >  1:540901/1‑149 (MQ=255)
                                                                   aTAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCa                                                                             <  4:415875/151‑1 (MQ=255)
                                                                     aGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCAt                                                                            <  3:396553/150‑1 (MQ=255)
                                                                       ccgccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCg                                                                          >  1:211325/1‑150 (MQ=255)
                                                                       ccgccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGa                                                                         <  1:1125549/151‑1 (MQ=255)
                                                                       ccgccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGa                                                                         <  4:439427/151‑1 (MQ=255)
                                                                        cgccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGAt                                                                        >  2:930052/1‑151 (MQ=255)
                                                                          ccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaa                                                                      <  1:552413/151‑1 (MQ=255)
                                                                          ccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaa                                                                      <  1:638167/151‑1 (MQ=255)
                                                                           cgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaac                                                                     <  4:353529/151‑1 (MQ=255)
                                                                               ggTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACt                                                                 <  4:22584/151‑1 (MQ=255)
                                                                               ggTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACt                                                                 >  1:197192/1‑151 (MQ=255)
                                                                                gTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTg                                                                <  1:626758/151‑1 (MQ=255)
                                                                                  tGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTgg                                                               >  4:147688/1‑150 (MQ=255)
                                                                                  tGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTgg                                                               >  4:309187/1‑150 (MQ=255)
                                                                                     aaaaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAgtg                                                           <  1:785193/151‑1 (MQ=255)
                                                                                     aaaaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAgtg                                                           >  4:445330/1‑151 (MQ=255)
                                                                                     aaaaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAgtg                                                           <  3:285107/151‑1 (MQ=255)
                                                                                      aaaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAgtgt                                                          >  2:813389/1‑151 (MQ=255)
                                                                                       aaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGtt                                                         >  3:346551/1‑151 (MQ=255)
                                                                                       aaCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGtt                                                         >  2:817854/1‑151 (MQ=255)
                                                                                          gTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGcc                                                      >  2:102431/1‑151 (MQ=255)
                                                                                           ttCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGccc                                                     >  2:1113874/1‑151 (MQ=255)
                                                                                            tCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGccc                                                     <  1:534536/150‑1 (MQ=255)
                                                                                              aCGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCcgcg                                                  <  1:618500/151‑1 (MQ=255)
                                                                                                  aCAGCGTCATAAACAGAGTCAACAACAACAACAGCCTGTTCAGAAGCACGGATTACGCCGTTGTTGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTa                                              <  1:410579/151‑1 (MQ=255)
                                                                                                  aCAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTa                                              <  1:818019/151‑1 (MQ=255)
                                                                                                     gCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTacac                                           >  1:859111/1‑151 (MQ=255)
                                                                                                         cATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGa                                        <  3:284867/150‑1 (MQ=255)
                                                                                                         cATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGa                                        <  1:246674/150‑1 (MQ=255)
                                                                                                         cATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGAt                                       >  2:409302/1‑151 (MQ=255)
                                                                                                             aaCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCt                                   <  2:692445/151‑1 (MQ=255)
                                                                                                              aCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTg                                  <  4:2717/151‑1 (MQ=255)
                                                                                                                agagTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGt                                >  3:126399/1‑151 (MQ=255)
                                                                                                                   gTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGttt                              >  3:425814/1‑150 (MQ=255)
                                                                                                                   gTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTa                             <  4:237155/151‑1 (MQ=255)
                                                                                                                     caacaacaacaaCAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTCCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTAc                            <  1:1198682/150‑1 (MQ=255)
                                                                                                                        caacaacaacaGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGa                        <  1:1181334/151‑1 (MQ=255)
                                                                                                                            aacaacaGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTg                    >  2:27866/1‑151 (MQ=255)
                                                                                                                              caacaGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGt                   >  4:386628/1‑150 (MQ=255)
                                                                                                                                  aGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCg              <  3:400400/151‑1 (MQ=255)
                                                                                                                                    aCTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGGTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGc            >  1:474411/1‑151 (MQ=255)
                                                                                                                                       gTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCt           <  2:738961/149‑1 (MQ=255)
                                                                                                                                          cAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAAc      >  1:742066/1‑151 (MQ=255)
                                                                                                                                          cAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAAc      <  1:606472/151‑1 (MQ=255)
                                                                                                                                           aGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAAcc     >  1:324708/1‑151 (MQ=255)
                                                                                                                                           aGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAAcc     >  2:179453/1‑151 (MQ=255)
                                                                                                                                           aGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAAcc     >  3:2340/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCGCGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATG  >  NC_000913/1297239‑1297531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑