Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,312,802 A→T I30N (ATC→AAC)  yciB ← IspA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,312,8020AT100.0% 448.3 / NA 112I30N (ATC→AAC) yciBIspA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (58/54);  total (58/54)

Only 100 of 116 total aligned reads displayed.

AACCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGATCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGG  >  NC_000913/1312657‑1312947
                                                                                                                                                 |                                                                                                                                                 
aaCCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAgc                                                                                                                                              >  1:880981/1‑151 (MQ=255)
aaCCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAgc                                                                                                                                              <  3:445426/151‑1 (MQ=255)
     tCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGcgccgc                                                                                                                                         >  4:336315/1‑151 (MQ=255)
      ccATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCa                                                                                                                                        <  3:222238/151‑1 (MQ=255)
      ccATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCa                                                                                                                                        <  2:367220/151‑1 (MQ=255)
       cATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGcgccgc                                                                                                                                         >  2:552340/1‑149 (MQ=255)
           taataaACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAg                                                                                                                                    <  4:229413/150‑1 (MQ=255)
           taataaACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGc                                                                                                                                   >  2:147611/1‑151 (MQ=255)
            aataaACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGcc                                                                                                                                  >  4:214179/1‑151 (MQ=255)
             ataaACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCg                                                                                                                                 >  4:289732/1‑151 (MQ=255)
                aaCTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCAt                                                                                                                              >  1:1202984/1‑151 (MQ=255)
                 aCTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATa                                                                                                                             <  2:972424/151‑1 (MQ=255)
                    catcatTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCAt                                                                                                                              >  1:1224412/1‑147 (MQ=255)
                    catcatTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAg                                                                                                                            <  1:1060244/149‑1 (MQ=255)
                    catcatTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGAt                                                                                                                          <  3:78696/151‑1 (MQ=255)
                    catcatTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGAt                                                                                                                          <  4:263481/151‑1 (MQ=255)
                         ttGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCAt                                                                                                                     >  2:687812/1‑151 (MQ=255)
                             tgaagaaCAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAAt                                                                                                                 >  2:545758/2‑151 (MQ=255)
                                agaaCAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCtt                                                                                                              <  1:332664/151‑1 (MQ=255)
                                 gaaCAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTg                                                                                                             >  4:222072/1‑151 (MQ=255)
                                   aCAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGt                                                                                                            >  2:1118565/1‑150 (MQ=255)
                                     aGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTaaa                                                                                                         <  3:135979/151‑1 (MQ=255)
                                     aGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTaaa                                                                                                         <  3:134078/151‑1 (MQ=255)
                                      gCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTaaaa                                                                                                        <  4:263572/151‑1 (MQ=255)
                                      gCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTaaa                                                                                                         >  1:238333/1‑150 (MQ=255)
                                       cGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAAc                                                                                                       <  2:54584/151‑1 (MQ=255)
                                         tCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAAcg                                                                                                      <  2:429421/150‑1 (MQ=255)
                                           aaGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAAcgcg                                                                                                    >  2:978465/1‑150 (MQ=255)
                                           aaGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAAcgcg                                                                                                    >  2:497693/1‑150 (MQ=255)
                                             gCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGaa                                                                                                  >  2:600138/1‑150 (MQ=255)
                                             gCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGaa                                                                                                  >  1:884501/1‑150 (MQ=255)
                                              ccACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGaaa                                                                                                 <  2:12695/150‑1 (MQ=255)
                                               cACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGaaaaa                                                                                               <  3:147166/151‑1 (MQ=255)
                                                 ccGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGa                                                                                             <  2:691944/151‑1 (MQ=255)
                                                  cGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGAc                                                                                            >  4:457919/1‑151 (MQ=255)
                                                  cGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGAc                                                                                            <  3:123834/151‑1 (MQ=255)
                                                    aaGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACa                                                                                           >  1:30399/1‑150 (MQ=255)
                                                    aaGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACa                                                                                           >  1:806391/1‑150 (MQ=255)
                                                      gacgacCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAAcc                                                                                        <  4:59293/151‑1 (MQ=255)
                                                       acgacCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAAcc                                                                                        >  1:1043665/1‑150 (MQ=255)
                                                       acgacCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCa                                                                                       <  2:955997/151‑1 (MQ=255)
                                                         gacCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGc                                                                                     >  2:526733/1‑151 (MQ=255)
                                                             accaGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTa                                                                                 <  2:1151415/151‑1 (MQ=255)
                                                               caGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaa                                                                                >  1:518990/1‑150 (MQ=255)
                                                                aGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaaaa                                                                              <  3:215848/151‑1 (MQ=255)
                                                                 gAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaaaa                                                                              >  1:444356/1‑150 (MQ=255)
                                                                  aaCAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaaaaaa                                                                            <  4:28827/151‑1 (MQ=255)
                                                                  aaCAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTaaaaa                                                                             <  2:1202769/150‑1 (MQ=255)
                                                                   aCAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAAt                                                                           <  1:656487/151‑1 (MQ=255)
                                                                    cAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGa                                                                                             <  1:505969/132‑1 (MQ=255)
                                                                     aaaaGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATc                                                                          >  2:57535/1‑150 (MQ=255)
                                                                       aaGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGGAAAAGACAACCAGCGGTAAAAAATCa                                                                         >  1:1103088/1‑149 (MQ=255)
                                                                       aaGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAg                                                                       <  1:1097808/151‑1 (MQ=255)
                                                                        aGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGa                                                                      <  3:382096/151‑1 (MQ=255)
                                                                            aTCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACt                                                                  <  1:723042/151‑1 (MQ=255)
                                                                              cAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGc                                                                >  2:310862/1‑151 (MQ=255)
                                                                               aGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCt                                                               >  2:174023/1‑151 (MQ=255)
                                                                               aGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCt                                                               >  4:416608/1‑151 (MQ=255)
                                                                               aGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCt                                                               >  2:830241/1‑151 (MQ=255)
                                                                                  gCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTc                                                             >  1:1239425/1‑150 (MQ=255)
                                                                                    cATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCAtt                                                          >  2:896788/1‑151 (MQ=255)
                                                                                     aTCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCAttt                                                         >  2:1050611/1‑151 (MQ=255)
                                                                                       cttctCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCAtttt                                                        >  3:83483/1‑150 (MQ=255)
                                                                                         tctcAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTa                                                       <  4:137371/149‑1 (MQ=255)
                                                                                         tctcAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTAc                                                      <  1:979616/150‑1 (MQ=255)
                                                                                         tctcAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTAc                                                      <  2:1019280/150‑1 (MQ=255)
                                                                                           tcAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGAt                                                   >  4:331597/1‑151 (MQ=255)
                                                                                            cAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGAtt                                                  >  4:357566/1‑151 (MQ=255)
                                                                                             aaCCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTc                                                 >  1:388980/1‑151 (MQ=255)
                                                                                              aCCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTcc                                                <  1:315904/151‑1 (MQ=255)
                                                                                                cTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGt                                              >  2:303633/1‑151 (MQ=255)
                                                                                                  tACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGt                                              <  1:552444/149‑1 (MQ=255)
                                                                                                      aaaGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCaa                                        <  1:168252/151‑1 (MQ=255)
                                                                                                      aaaGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCa                                         >  2:1067516/1‑150 (MQ=255)
                                                                                                            aaCCCAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCtt                                  <  1:333720/151‑1 (MQ=255)
                                                                                                              cccAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTa                                 <  1:352533/150‑1 (MQ=255)
                                                                                                               ccAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTa                                 >  1:776624/1‑149 (MQ=255)
                                                                                                                cAGCTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAgg                              >  1:899065/1‑151 (MQ=255)
                                                                                                                  gCTATAGATAAGAACAAGCGCCGTGGAGACGTTCCGCGCCGCAGAAGCAGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTACCTCTTACGATTCCGTAATCAAATGCTTAAGGtt                            <  3:242405/151‑1 (MQ=255)
                                                                                                                   cTATAGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGtt                            >  2:797234/1‑150 (MQ=255)
                                                                                                                    tataGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTAt                          >  4:289874/1‑151 (MQ=255)
                                                                                                                     ataGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTAtt                         <  1:190445/151‑1 (MQ=255)
                                                                                                                       aGATAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTg                        <  4:440498/150‑1 (MQ=255)
                                                                                                                         aTAAGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCg                     >  1:540630/1‑151 (MQ=255)
                                                                                                                           aaGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGa                    >  3:389629/1‑150 (MQ=255)
                                                                                                                           aaGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGa                    >  2:455177/1‑150 (MQ=255)
                                                                                                                            aGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAAt                  <  1:545859/151‑1 (MQ=255)
                                                                                                                            aGCACAATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAAt                  <  1:1210724/151‑1 (MQ=255)
                                                                                                                               acaATCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCa                >  3:179438/1‑150 (MQ=255)
                                                                                                                                  aTCGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACat            <  3:76390/151‑1 (MQ=255)
                                                                                                                                    cGCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAAACAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACata           <  4:406969/150‑1 (MQ=255)
                                                                                                                                     gCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACAtata         <  1:163837/151‑1 (MQ=255)
                                                                                                                                     gCCGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACAtata         <  1:237045/151‑1 (MQ=255)
                                                                                                                                      ccGTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATAc        >  2:1284204/1‑151 (MQ=255)
                                                                                                                                        gTGGCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAg      <  2:549056/151‑1 (MQ=255)
                                                                                                                                           gCGACGTTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCg   <  3:249567/151‑1 (MQ=255)
                                                                                                                                            cgacgtTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCgg  <  2:518897/151‑1 (MQ=255)
                                                                                                                                            cgacgtTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCgg  <  3:395427/151‑1 (MQ=255)
                                                                                                                                            cgacgtTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCgg  >  2:1180845/1‑151 (MQ=255)
                                                                                                                                             gacgtTCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCgg  >  4:431787/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AACCTTCCATTTAATAAACTCATCATTGTGGAAGAACAGCGTCAAGCCACCGAAGACGACCACCAGAACAAAAGTGATCAGGGCCATCTTCTCAACCTTACGAAAGCGAACCCAGCTATAGATAAGCACAATCGCCGTGGCGACGATCAGCGCCGCAGTAGCCGCATAGATGTCATAAATCTTGTAAAACGCGAAAAAGACAACCAGCGGTAAAAAATCAAGAAACTGCTTCATTTTACGATTCCGTAATCAAATGCTTAAGGTTATTGGCGAATCAACATATACAGGCGG  >  NC_000913/1312657‑1312947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑