Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,437,422 T→C A1121A (GCA→GCG)  pfo ← pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,437,4220TC99.1% 389.4 / ‑4.4 108A1121A (GCA→GCG) pfopyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (56/51);  total (57/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 108 total aligned reads displayed.

TTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGTGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTC  >  NC_000913/1437278‑1437568
                                                                                                                                                |                                                                                                                                                  
ttCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGCTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTc                                                                                                                                               >  2:747900/1‑150 (MQ=255)
 tCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCt                                                                                                                                              <  2:1111003/150‑1 (MQ=255)
  ccGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTg                                                                                                                                             <  2:435951/150‑1 (MQ=255)
  ccGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTg                                                                                                                                             >  3:83898/1‑150 (MQ=255)
   cGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGAc                                                                                                                                           >  3:120110/1‑151 (MQ=255)
   cGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGAc                                                                                                                                           >  2:30669/1‑151 (MQ=255)
    gCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGAcg                                                                                                                                          <  4:235449/151‑1 (MQ=255)
       tttCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGAcggc                                                                                                                                        <  1:427128/150‑1 (MQ=255)
       tttCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGAcgg                                                                                                                                         <  2:302671/149‑1 (MQ=255)
         tCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACggcggg                                                                                                                                     >  3:318131/1‑151 (MQ=255)
          ccGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACggcgggc                                                                                                                                    <  2:426508/151‑1 (MQ=255)
          ccGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACggcggg                                                                                                                                     <  1:21715/150‑1 (MQ=255)
           cGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACggcgggcg                                                                                                                                   <  2:662098/151‑1 (MQ=255)
               cATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGa                                                                                                                                >  2:19255/1‑150 (MQ=255)
                   tgtgCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATcc                                                                                                                            <  1:9654/150‑1 (MQ=255)
                        cAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGcc                                                                                                                      >  4:239164/1‑151 (MQ=255)
                          ggAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAg                                                                                                                    <  2:1235398/151‑1 (MQ=255)
                          ggAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAg                                                                                                                    <  1:115600/151‑1 (MQ=255)
                            aaGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCg                                                                                                                  <  2:346579/151‑1 (MQ=255)
                            aaGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCg                                                                                                                  <  3:12601/151‑1 (MQ=255)
                             tgTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCgg                                                                                                                 >  2:48645/2‑151 (MQ=255)
                               tCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGc                                                                                                                >  2:1082111/1‑150 (MQ=255)
                                 aTAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAg                                                                                                              >  1:738956/1‑150 (MQ=255)
                                 aTAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGt                                                                                                             >  1:1139818/1‑151 (MQ=255)
                                 aTAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGt                                                                                                             >  4:267211/1‑151 (MQ=255)
                                   aGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGttt                                                                                                           >  1:449139/1‑151 (MQ=255)
                                           tGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCa                                                                                                    <  2:636005/150‑1 (MQ=255)
                                            gCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATc                                                                                                  >  1:508047/1‑151 (MQ=255)
                                            gCAAAGCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCAt                                                                                                   <  3:377564/150‑1 (MQ=255)
                                              aaaTCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCgg                                                                                                <  1:556531/151‑1 (MQ=255)
                                              aaaTCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCgg                                                                                                <  2:641406/151‑1 (MQ=255)
                                              aaaTCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCgg                                                                                                >  1:42420/1‑151 (MQ=255)
                                               aaTCAGCTGCAGCATCTTTCCATAACTGTACTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGc                                                                                               <  1:422515/151‑1 (MQ=255)
                                                 tCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGAAAACGTTGCTCGTGAAGTAACGTTTCTTCCGTCGCTTCTGACGGCGGGCGAGAATCCAAGGCCAGCGGCAAGTTGCCTTCAt                                                                                                   >  1:331619/1‑145 (MQ=255)
                                                  cAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCacg                                                                                            >  2:994327/1‑151 (MQ=255)
                                                             tCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCTGCCGTCGGAAACGTTGCTCGTGATGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGCGAATCCAAGGCCAGCGGCAGTTTGTCTTCCTCGGCacggcg                                                                                         >  2:1105486/1‑143 (MQ=255)
                                                             tCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGACAGTTTGCCTTCATCGGCACGACGCGGATCa                                                                                  >  2:869116/1‑150 (MQ=255)
                                                                ttCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCa                                                                                  <  2:1183210/147‑1 (MQ=255)
                                                                 tCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGc                                                                              <  1:694256/150‑1 (MQ=255)
                                                                  ccATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCg                                                                             >  2:267439/1‑150 (MQ=255)
                                                                   cATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGat                                                                           <  1:207893/151‑1 (MQ=255)
                                                                      aaCTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGatat                                                                         >  1:300650/1‑150 (MQ=255)
                                                                      aaCTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGata                                                                          >  2:1100234/1‑149 (MQ=255)
                                                                       aCTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAg                                                                       <  1:625391/151‑1 (MQ=255)
                                                                        cTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAg                                                                       >  1:663344/1‑150 (MQ=255)
                                                                          gTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCgg                                                                    >  4:10644/1‑151 (MQ=255)
                                                                          gTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCgg                                                                    <  2:1159540/151‑1 (MQ=255)
                                                                          gTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCgg                                                                    >  1:137139/1‑151 (MQ=255)
                                                                          gTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCg                                                                     <  3:238254/150‑1 (MQ=255)
                                                                           ttCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCgg                                                                    >  2:598793/1‑150 (MQ=255)
                                                                            tCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGcc                                                                  >  4:329959/1‑151 (MQ=255)
                                                                              tGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGcc                                                                  >  1:736895/1‑149 (MQ=255)
                                                                               gCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCa                                                                 <  1:821639/149‑1 (MQ=255)
                                                                                ccACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGaa                                                              <  2:983046/151‑1 (MQ=255)
                                                                                ccACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGaa                                                              <  2:1218409/151‑1 (MQ=255)
                                                                                ccACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGa                                                               >  2:15981/1‑150 (MQ=255)
                                                                                  aCTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCCCGACGCGGATCAAAGCGATATAGCGGCCAGAAGc                                                            >  2:565402/1‑151 (MQ=255)
                                                                                  aCTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGaa                                                              <  1:396014/149‑1 (MQ=255)
                                                                                    ttCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCCGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGcc                                                           >  2:583716/1‑150 (MQ=255)
                                                                                    ttCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGGCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGcc                                                           >  3:436383/1‑150 (MQ=255)
                                                                                    ttCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGcc                                                           >  2:1032077/1‑150 (MQ=255)
                                                                                    ttCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCg                                                          >  4:203816/1‑151 (MQ=255)
                                                                                     tCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGcc                                                           <  1:554870/149‑1 (MQ=255)
                                                                                       tGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAAGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGAAATAGCGGCCAGAAGCCGGt                                                        >  1:727454/1‑150 (MQ=255)
                                                                                        ggCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGt                                                        <  2:708640/149‑1 (MQ=255)
                                                                                        ggCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTa                                                       <  4:120111/150‑1 (MQ=255)
                                                                                        ggCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAg                                                      <  1:852390/151‑1 (MQ=255)
                                                                                        ggCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGACAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCTGt                                                        >  1:201039/1‑149 (MQ=255)
                                                                                         gctgctGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGGTCCAAGCGATATAGCGGCCAGAAGCCGGt                                                        >  3:144347/1‑148 (MQ=255)
                                                                                          ctgctgCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCt                                                    >  3:359111/1‑151 (MQ=255)
                                                                                            gctgcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCtg                                                   >  1:1041100/1‑150 (MQ=255)
                                                                                            gctgcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCt                                                    <  1:172594/149‑1 (MQ=255)
                                                                                            gctgcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTAAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCtgt                                                  <  2:704231/151‑1 (MQ=255)
                                                                                             ctgcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCtgtg                                                 >  3:137113/1‑151 (MQ=255)
                                                                                              tgcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCtgtg                                                 >  2:740728/1‑150 (MQ=255)
                                                                                               gcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCtgtg                                                 <  2:859294/149‑1 (MQ=255)
                                                                                               gcGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCtgtg                                                 <  2:716788/149‑1 (MQ=255)
                                                                                                      cAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGgcgc                                        >  1:146153/1‑151 (MQ=255)
                                                                                                      cAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGgcgc                                        >  1:625920/1‑151 (MQ=255)
                                                                                                      cAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTGCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGgcgc                                        <  1:497521/151‑1 (MQ=255)
                                                                                                          cGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCt                                    >  4:36520/1‑151 (MQ=255)
                                                                                                           gACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGTGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTg                                   >  4:163395/1‑151 (MQ=255)
                                                                                                           gACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTg                                   <  1:37089/151‑1 (MQ=255)
                                                                                                            aCGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTgg                                  >  2:311322/1‑151 (MQ=255)
                                                                                                              ggAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTc                                >  1:237782/1‑151 (MQ=255)
                                                                                                              ggAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTc                                >  1:1272797/1‑151 (MQ=255)
                                                                                                              ggAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTc                                <  2:1126544/151‑1 (MQ=255)
                                                                                                                 aaCGTTGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTg                             <  1:427913/151‑1 (MQ=255)
                                                                                                                      tGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTg                         >  1:1199511/1‑150 (MQ=255)
                                                                                                                      tGCTCGTGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTg                         <  1:730982/150‑1 (MQ=255)
                                                                                                                            tGAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGcc                   >  1:380098/1‑150 (MQ=255)
                                                                                                                             gAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGcc                   >  2:1112810/1‑149 (MQ=255)
                                                                                                                             gAAGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAg                 <  1:19257/151‑1 (MQ=255)
                                                                                                                              aaGTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGcc                   >  4:402228/1‑148 (MQ=255)
                                                                                                                                gTAACGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATc              <  1:568715/151‑1 (MQ=255)
                                                                                                                                   aCGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTa           <  1:114179/151‑1 (MQ=255)
                                                                                                                                    cGTTTCTTCCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTaa          <  2:241339/151‑1 (MQ=255)
                                                                                                                                       ttcttcCGGCGCTTCTGACGGCGGGCCGGAATCCAAGGCCAGCGGCAGGTTGCCTTCATCGGCCCGACGCGGATCAAAGCGATATAGCGGCCAGAAGGCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCCGAGTGCCAGATCGTAAcc        >  1:477266/1‑150 (MQ=255)
                                                                                                                                           tcCGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCAt      <  2:227385/148‑1 (MQ=255)
                                                                                                                                            ccGGCGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGctc  <  2:91265/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCACTTCTGGCTGCTGCGAATTCAGCCGACGGAAACGTTGCTCGTGAAGTAACGTTTCTTCCGGTGCTTCTGACGGCGGGCGTGAATCCAAGGCCAGCGGCAGTTTGCCTTCATCGGCACGACGCGGATCAAAGCGATATAGCGGCCAGAAGCCGGTAGCTGTGAGTTGGCGCATCTGGTCGTGGCTGAGTGCCAGATCGTAACCATGCTC  >  NC_000913/1437278‑1437568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑