Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,836	T→C	L151P (CTG→CCG)	yeaI →	putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,836	0	T	C	99.0%	368.4 / ‑3.9	99	L151P (CTG→CCG)	yeaI	putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (46/52);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGC  >  NC_000913/1870691‑1870983                                                                                                                                                  |                                                                                                                                                    gTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATcc                                                                                                                                                >  1:283675/1‑151 (MQ=255)   cATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAg                                                                                                                                              <  2:1034787/151‑1 (MQ=255)    aTTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATcc                                                                                                                                                >  1:496280/1‑148 (MQ=255)    aTTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATcc                                                                                                                                                >  1:1110597/1‑148 (MQ=255)    aTTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGa                                                                                                                                             <  1:150252/151‑1 (MQ=255)     tttttCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGAc                                                                                                                                            >  1:80948/1‑151 (MQ=255)        ttcttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATCATCCATAAACCAATTAACGGCAGTACACCGATCCAGACaa                                                                                                                                          >  2:342804/1‑150 (MQ=255)          cttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGt                                                                                                                                       >  1:222658/1‑151 (MQ=255)            tttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAg                                                                                                                                        <  2:269713/148‑1 (MQ=255)             ttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCg                                                                                                                                     >  2:96759/1‑150 (MQ=255)             ttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCg                                                                                                                                     >  3:51995/1‑150 (MQ=255)               cATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGaa                                                                                                                                   <  2:1083074/150‑1 (MQ=255)               cATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAat                                                                                                                                  <  1:1169011/151‑1 (MQ=255)                aTGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatg                                                                                                                                 <  2:421947/151‑1 (MQ=255)                aTGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatg                                                                                                                                 <  2:968036/151‑1 (MQ=255)                aTGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatg                                                                                                                                 <  2:1159538/151‑1 (MQ=255)                aTGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatg                                                                                                                                 >  4:96852/1‑151 (MQ=255)                  gTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatgat                                                                                                                               >  2:657438/1‑151 (MQ=255)                  gTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatga                                                                                                                                <  1:1063465/150‑1 (MQ=255)                   ttAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatgatg                                                                                                                              >  2:1185981/1‑151 (MQ=255)                   ttAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAatgatg                                                                                                                              >  4:75274/1‑151 (MQ=255)                     aaTGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtt                                                                                                                            >  1:242616/1‑151 (MQ=255)                      aTGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGttt                                                                                                                           <  2:833448/151‑1 (MQ=255)                       tGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttc                                                                                                                          >  4:436590/1‑151 (MQ=255)                        gAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttct                                                                                                                         <  1:1089377/151‑1 (MQ=255)                        gAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttc                                                                                                                          <  2:716637/150‑1 (MQ=255)                          aaTATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctat                                                                                                                       <  2:627461/151‑1 (MQ=255)                           atatTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctatt                                                                                                                      <  2:147520/151‑1 (MQ=255)                           atatTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctatt                                                                                                                      >  2:30193/1‑151 (MQ=255)                             atTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctatttt                                                                                                                    >  1:735690/1‑151 (MQ=255)                             atTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctatttt                                                                                                                    >  1:761581/1‑151 (MQ=255)                               ttCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttc                                                                                                                   <  2:656056/150‑1 (MQ=255)                               ttCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttc                                                                                                                   <  2:303904/150‑1 (MQ=255)                                tCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttct                                                                                                                  <  2:431242/150‑1 (MQ=255)                                 cACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttctat                                                                                                                <  1:746600/151‑1 (MQ=255)                                 cACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttctat                                                                                                                <  1:1213869/151‑1 (MQ=255)                                  aCTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGtttctattttctat                                                                                                                >  1:369268/1‑150 (MQ=255)                                     aacaaacaAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                                                                            <  2:223938/151‑1 (MQ=255)                                      acaaacaaATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAtttt                                                                                                           <  1:982709/151‑1 (MQ=255)                                       caaacaaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTc                                                                                                          <  1:295079/151‑1 (MQ=255)                                        aaacaaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTcc                                                                                                         <  1:96773/151‑1 (MQ=255)                                         aacaaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCg                                                                                                        <  1:704834/151‑1 (MQ=255)                                          acaaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGc                                                                                                       <  2:766844/151‑1 (MQ=255)                                          acaaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGc                                                                                                       <  1:1258012/151‑1 (MQ=255)                                             acttttACCAACGCTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTCGTTGTTATCTTTGCAATTATCCATAAATCAATTATCAGCAGTACACCGATCATGTCAAAGTCGTATGATGTTTATATTTTCTATAtttt                                                                                                           >  2:293604/3‑144 (MQ=255)                                               ttttACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACt                                                                                                  >  1:315312/1‑151 (MQ=255)                                                gttACCAACACTCATTTTAAGGGTTGCATTTTTAGGTGTCCTTATCTATTTAGTTGGAACCCTTGTAATTATAAATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTc                                                                                                 >  2:394676/2‑151 (MQ=255)                                                   aCCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAg                                                                                               <  1:605860/150‑1 (MQ=255)                                                      aaCACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGtttt                                                                                           <  1:558853/151‑1 (MQ=255)                                                            cATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTg                                                                                      <  1:474788/150‑1 (MQ=255)                                                            cATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTg                                                                                      <  1:889707/150‑1 (MQ=255)                                                            cATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGt                                                                                     >  1:1248835/1‑151 (MQ=255)                                                               tttAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGttt                                                                                   >  4:164442/1‑150 (MQ=255)                                                                  aaGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAAcc                                                                               <  1:1025961/151‑1 (MQ=255)                                                                    gCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTc                                                                             >  2:554219/1‑151 (MQ=255)                                                                     ccTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTC‑GGTTTATTTTTTTaaac                                                                               >  2:651008/1‑145 (MQ=255)                                                                      cTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGc                                                                           >  4:444551/1‑151 (MQ=255)                                                                         gCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTgg                                                                        >  1:604353/1‑151 (MQ=255)                                                                          cATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCGTTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgc                                                                       <  2:435281/151‑1 (MQ=255)                                                                             ttttAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgcgc                                                                     <  2:77402/150‑1 (MQ=255)                                                                              tttAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGctc                                                                   >  2:959521/1‑151 (MQ=255)                                                                              tttAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGctc                                                                   >  4:95673/1‑151 (MQ=255)                                                                                tAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCtt                                                                 >  2:342514/1‑151 (MQ=255)                                                                                 aaGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCttt                                                                >  3:418950/1‑151 (MQ=255)                                                                                    tGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGt                                                             >  1:69576/1‑151 (MQ=255)                                                                                    tGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGt                                                             >  4:11210/1‑151 (MQ=255)                                                                                     ggCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGt                                                             <  1:964878/150‑1 (MQ=255)                                                                                       ccTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTAt                                                          >  2:673643/1‑151 (MQ=255)                                                                                       ccTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTAt                                                          <  1:554322/151‑1 (MQ=255)                                                                                           aTCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGa                                                       >  4:201629/1‑150 (MQ=255)                                                                                                tttAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGac                                                 >  4:318722/1‑151 (MQ=255)                                                                                                  tAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGaca                                                >  1:778626/1‑150 (MQ=255)                                                                                                    gTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGacaaca                                             <  4:77425/151‑1 (MQ=255)                                                                                                      tGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATc                                           >  2:348914/1‑151 (MQ=255)                                                                                                       gAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATc                                           <  2:1251097/150‑1 (MQ=255)                                                                                                         aaCCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCtt                                        <  2:242578/151‑1 (MQ=255)                                                                                                          aCCATTGTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGCTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTg                                       >  1:445928/1‑151 (MQ=255)                                                                                                                gTAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaaca                                 <  2:810714/151‑1 (MQ=255)                                                                                                                 tAATTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGacaaca                                 >  4:418796/1‑150 (MQ=255)                                                                                                                   aTTATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATa                              <  2:93684/151‑1 (MQ=255)                                                                                                                     tATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATa                              >  2:1171998/1‑149 (MQ=255)                                                                                                                     tATCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACACAGTTTTATTTGTTTAACCTCTCTGTCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACACTaa                             <  2:63445/150‑1 (MQ=255)                                                                                                                       tCCATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGa                           >  2:509781/1‑150 (MQ=255)                                                                                                                        ccATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaa                          <  2:604232/150‑1 (MQ=255)                                                                                                                        ccATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaa                          <  2:1120215/150‑1 (MQ=255)                                                                                                                         cATAAACCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaaaa                        <  1:598049/151‑1 (MQ=255)                                                                                                                            aaaCCAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGaaaaaaa                     <  2:351502/151‑1 (MQ=255)                                                                                                                                cAATTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACggg                 <  1:926670/151‑1 (MQ=255)                                                                                                                                 aaTTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGa                <  2:835456/151‑1 (MQ=255)                                                                                                                                 aaTTAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGa                <  2:294153/151‑1 (MQ=255)                                                                                                                                   ttAACGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAAt              >  1:436501/1‑151 (MQ=255)                                                                                                                                     aaCGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATcc            >  2:866811/1‑151 (MQ=255)                                                                                                                                      aCGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCt           <  2:890798/151‑1 (MQ=255)                                                                                                                                      aCGGCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCt           <  2:267288/151‑1 (MQ=255)                                                                                                                                        ggCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt         <  1:689907/151‑1 (MQ=255)                                                                                                                                         gCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGtt        <  4:305860/151‑1 (MQ=255)                                                                                                                                         gCAGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGtt        >  1:858636/1‑151 (MQ=255)                                                                                                                                           aGTACACCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGc      >  1:468657/1‑151 (MQ=255)                                                                                                                                               cacCGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGgc  >  2:1188900/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGC  >  NC_000913/1870691‑1870983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑