Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,876,662	G→A	A330A (GCG→GCA)	yeaN →	putative MFS transporter, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,662	0	G	A	100.0%	357.2 / NA	91	A330A (GCG→GCA)	yeaN	putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/44);  total (47/44)

GTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATG  >  NC_000913/1876515‑1876809                                                                                                                                                    |                                                                                                                                                    gttcgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGcaca                                                                                                                                                   >  2:654246/1‑150 (MQ=255)    cgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATc                                                                                                                                                 >  3:403116/1‑149 (MQ=255)    cgttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCa                                                                                                                                                <  2:423751/150‑1 (MQ=255)      ttGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAggc                                                                                                                                             >  1:28602/1‑151 (MQ=255)       tGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAggc                                                                                                                                             <  2:500381/150‑1 (MQ=255)        gCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAgacgg                                                                                                                                           <  4:133796/151‑1 (MQ=255)          cTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCTGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAggcggc                                                                                                                                          <  1:748816/150‑1 (MQ=255)            tGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAggcggcgg                                                                                                                                        <  3:449114/150‑1 (MQ=255)             gATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAggcggcggc                                                                                                                                       <  1:1258536/150‑1 (MQ=255)                  gcgcAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCActct                                                                                                                                  <  1:31340/150‑1 (MQ=255)                  gcgcAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACtctc                                                                                                                                 <  4:409729/151‑1 (MQ=255)                  gcgcAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACtctc                                                                                                                                 <  2:503985/151‑1 (MQ=255)                   cgcAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCg                                                                                                                                <  4:198579/151‑1 (MQ=255)                    gcAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCgg                                                                                                                               >  4:142784/1‑151 (MQ=255)                      aGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCgggg                                                                                                                             >  2:1256285/1‑151 (MQ=255)                       gTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGa                                                                                                                            <  2:424230/151‑1 (MQ=255)                         gggCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATg                                                                                                                          <  2:336071/151‑1 (MQ=255)                           gcgcGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGc                                                                                                                        <  1:908355/151‑1 (MQ=255)                           gcgcGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGc                                                                                                                        >  2:394062/1‑151 (MQ=255)                            cgcgGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGc                                                                                                                        >  4:358489/1‑150 (MQ=255)                               ggTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGcaca                                                                                                                     >  3:416617/1‑150 (MQ=255)                                gTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAAt                                                                                                                   <  2:549994/151‑1 (MQ=255)                                gTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAAt                                                                                                                   <  1:577779/151‑1 (MQ=255)                                 ttGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATc                                                                                                                  >  3:196958/1‑151 (MQ=255)                                  tGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATc                                                                                                                  >  2:345020/1‑150 (MQ=255)                                        ctGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCggg                                                                                                           >  3:276799/1‑151 (MQ=255)                                          gCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCgg                                                                                                            <  2:158480/148‑1 (MQ=255)                                             ttATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTAttt                                                                                                      >  4:277869/1‑151 (MQ=255)                                             ttATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTAtt                                                                                                       <  1:1207160/150‑1 (MQ=255)                                              tATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTAttt                                                                                                      >  2:799427/1‑150 (MQ=255)                                                 gCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATttgttg                                                                                                  <  2:638387/151‑1 (MQ=255)                                                  ccAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTgg                                                                                                 >  1:783496/1‑151 (MQ=255)                                                      cgcACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGc                                                                                             >  1:138722/1‑151 (MQ=255)                                                       gcACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGcc                                                                                            >  3:116408/1‑151 (MQ=255)                                                       gcACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGcc                                                                                            >  1:312112/1‑151 (MQ=255)                                                          cgcgATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCtgt                                                                                         <  1:545334/151‑1 (MQ=255)                                                           gcgATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCtgtg                                                                                        <  1:598817/151‑1 (MQ=255)                                                            cgATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTCGTTGGCAGCCTGTgg                                                                                       <  2:520329/151‑1 (MQ=255)                                                             gATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTggg                                                                                      >  2:386201/1‑151 (MQ=255)                                                                cACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGgccg                                                                                   >  1:367853/1‑151 (MQ=255)                                                                   cTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGccgccg                                                                                >  2:1206097/1‑151 (MQ=255)                                                                       aCTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTa                                                                                                         >  3:362093/1‑122 (MQ=255)                                                                       aCTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTa                                                                                                         <  4:362099/122‑1 (MQ=255)                                                                         tctGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATg                                                                          >  1:866157/1‑151 (MQ=255)                                                                             cTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATggg                                                                        >  2:511463/1‑149 (MQ=255)                                                                               tttCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTaa                                                                     <  1:1019681/150‑1 (MQ=255)                                                                                 tCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTaaaa                                                                   <  4:397764/150‑1 (MQ=255)                                                                                 tCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAAt                                                                  >  4:169898/1‑151 (MQ=255)                                                                                  cgtttttGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATGCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAAt                                                                  <  2:370716/147‑1 (MQ=255)                                                                                  cGGTTTTGGTACCGGCGCAACAATGATACTGGGATATACGTTCATTGGGCTGCGGTCTTGTTCTGCACATGAGGCGGCGGCGCTCTCGGGGATGGCACAATCCGTCGGGTATTTGTGGGCCGCCTGTGGGCCGGCGGTGATGGGTAAAAt                                                                  <  2:324777/150‑1 (MQ=255)                                                                                   ggTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATac                                                                >  1:1255736/1‑151 (MQ=255)                                                                                    gTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATac                                                                >  4:276379/1‑150 (MQ=255)                                                                                    gTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATac                                                                >  2:902229/1‑150 (MQ=255)                                                                                      tttGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACg                                                             >  3:370224/1‑151 (MQ=255)                                                                                      tttGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACg                                                             >  1:56181/1‑151 (MQ=255)                                                                                        tGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGAt                                                           >  2:127213/1‑151 (MQ=255)                                                                                            tCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCt                                                        >  2:775216/1‑150 (MQ=255)                                                                                             ccGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTaa                                                      <  1:780608/151‑1 (MQ=255)                                                                                             ccGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTa                                                       <  2:323869/150‑1 (MQ=255)                                                                                                gcgcAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACgg                                                   <  2:448007/151‑1 (MQ=255)                                                                                                  gcAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTa                                                 <  4:155037/151‑1 (MQ=255)                                                                                                  gcAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTa                                                 <  1:1053317/151‑1 (MQ=255)                                                                                                   caacaaTGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTa                                                 >  4:154055/1‑150 (MQ=255)                                                                                                    aacaaTGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAAc                                               >  2:958103/1‑151 (MQ=255)                                                                                                       aaTGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTg                                             <  1:105776/150‑1 (MQ=255)                                                                                                          gATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCt                                         >  1:778685/1‑151 (MQ=255)                                                                                                           aTACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTg                                        <  4:181712/151‑1 (MQ=255)                                                                                                             aCTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGt                                       <  1:1076073/150‑1 (MQ=255)                                                                                                              cTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTAGTCTGt                                       <  1:149449/149‑1 (MQ=255)                                                                                                               tGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTAcc                                    >  3:435987/1‑151 (MQ=255)                                                                                                                ggggTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCa                                   <  2:1082047/151‑1 (MQ=255)                                                                                                                   gTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACtt                                <  4:49674/151‑1 (MQ=255)                                                                                                                       aCGTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATgg                            >  4:104716/1‑151 (MQ=255)                                                                                                                         gTTCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCCGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATggg                           <  2:81053/150‑1 (MQ=255)                                                                                                                           tCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGgtgt                        >  1:40275/1‑151 (MQ=255)                                                                                                                           tCATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGgtg                         <  1:1144884/150‑1 (MQ=255)                                                                                                                            cATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGtt                       <  3:408546/151‑1 (MQ=255)                                                                                                                            cATTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGtt                       <  1:187507/151‑1 (MQ=255)                                                                                                                             aTTGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTg                      >  1:596987/1‑151 (MQ=255)                                                                                                                               tGGTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGc                     <  4:124518/150‑1 (MQ=255)                                                                                                                                 gTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCAt                  >  2:890634/1‑151 (MQ=255)                                                                                                                                 gTCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCAt                  >  4:291802/1‑151 (MQ=255)                                                                                                                                  tatGCGGGCTAGTTCTGCACATCAGTCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTCACGGTAACTGGTCTGTACCACTTATGGGTGTTGCTAt                  >  3:52509/3‑150 (MQ=255)                                                                                                                                  tCTGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCAt                  >  2:276714/1‑150 (MQ=255)                                                                                                                                    tGCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACt               >  2:542497/1‑151 (MQ=255)                                                                                                                                     gCGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATa                 <  1:190243/148‑1 (MQ=255)                                                                                                                                      cGGGCTAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACttt             >  2:127916/1‑151 (MQ=255)                                                                                                                                           tAGTTCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGCCTGTACCACTTATGGGTGTTGCCATACTTTc            >  1:57017/1‑147 (MQ=255)                                                                                                                                               tCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTg     >  2:45310/1‑150 (MQ=255)                                                                                                                                               tCTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTg     <  1:181996/150‑1 (MQ=255)                                                                                                                                                cTGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGGGTTGCCATACTTTCACTACTGa    >  1:829456/1‑150 (MQ=255)                                                                                                                                                 tGCACATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATg  >  2:620420/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCACAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGATGCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATG  >  NC_000913/1876515‑1876809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑