Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,954,322 T→C L294P (CTG→CCG)  cmoB → tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,954,3220TC99.0% 335.0 / NA 98L294P (CTG→CCG) cmoBtRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (50/47);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 101 total aligned reads displayed.

CAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAA  >  NC_000913/1954174‑1954464
                                                                                                                                                    |                                                                                                                                              
cAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCgg                                                                                                                                              >  2:257965/1‑151 (MQ=255)
  aaTGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGcc                                                                                                                                            <  1:260706/151‑1 (MQ=255)
  aaTGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGcc                                                                                                                                            <  2:910442/151‑1 (MQ=255)
   aTGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCgccggccg                                                                                                                                           >  1:171810/1‑151 (MQ=255)
    tGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCgccggccg                                                                                                                                           >  2:1160263/1‑150 (MQ=255)
    tGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCgccggccg                                                                                                                                           >  2:80732/1‑150 (MQ=255)
      cGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGAtt                                                                                                                                        >  1:866572/1‑151 (MQ=255)
      cGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGAtt                                                                                                                                        >  1:1116764/1‑151 (MQ=255)
        tAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTc                                                                                                                                      >  1:1215467/1‑151 (MQ=255)
            gTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTc                                                                                                                                   <  4:159533/150‑1 (MQ=255)
            gTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTc                                                                                                                                   <  1:148358/150‑1 (MQ=255)
            gTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTc                                                                                                                                   <  1:1102911/150‑1 (MQ=255)
               tATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGAcc                                                                                                                               >  2:878066/1‑151 (MQ=255)
                 tttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGAccc                                                                                                                              >  1:67508/1‑150 (MQ=255)
                  ttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCcac                                                                                                                            >  2:560268/1‑151 (MQ=255)
                  ttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCcac                                                                                                                            >  4:444572/1‑151 (MQ=255)
                  ttCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCcac                                                                                                                            >  3:181675/1‑151 (MQ=255)
                    cATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACAt                                                                                                                          >  2:526543/1‑151 (MQ=255)
                    cATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACAt                                                                                                                          >  1:837451/1‑151 (MQ=255)
                       cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGAt                                                                                                                       >  1:1061454/1‑151 (MQ=255)
                             cgcgCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGt                                                                                                                 >  2:1278163/1‑151 (MQ=255)
                              gcgctggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCggg                                                                                                                  >  2:574545/1‑149 (MQ=255)
                              gcgctggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGt                                                                                                                 >  1:18309/1‑150 (MQ=255)
                                   ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTaaaa                                                                                                             <  1:327290/149‑1 (MQ=255)
                                   ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACg                                                                                                           >  3:104344/1‑151 (MQ=255)
                                    gcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACgg                                                                                                          >  2:820138/1‑151 (MQ=255)
                                      gctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAGCGGTg                                                                                                        <  2:157342/151‑1 (MQ=255)
                                        tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTgg                                                                                                       >  4:19312/1‑150 (MQ=255)
                                         gAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGa                                                                                                      <  1:779695/150‑1 (MQ=255)
                                           aaaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAgg                                                                                                   >  3:344685/1‑151 (MQ=255)
                                             aaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGtt                                                                                                 <  4:181677/151‑1 (MQ=255)
                                                 ggCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTAt                                                                                               <  1:134337/149‑1 (MQ=255)
                                                 ggCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATcc                                                                                             >  1:436377/1‑151 (MQ=255)
                                                 ggCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATcc                                                                                             <  2:333255/151‑1 (MQ=255)
                                                   cTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCgg                                                                                           >  1:654928/1‑151 (MQ=255)
                                                   cTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCg                                                                                            <  2:535699/150‑1 (MQ=255)
                                                      aagaagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCAc                                                                                        <  2:286835/151‑1 (MQ=255)
                                                        gaagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCt                                                                                      >  2:930375/1‑151 (MQ=255)
                                                        gaagTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCt                                                                                      <  2:1098676/151‑1 (MQ=255)
                                                            tgtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAc                                                                                  >  2:970641/1‑151 (MQ=255)
                                                            tgtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAc                                                                                  >  1:1186910/1‑151 (MQ=255)
                                                             gtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcg                                                                                 <  1:758877/151‑1 (MQ=255)
                                                              tgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgc                                                                                >  4:246383/1‑151 (MQ=255)
                                                               ggTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                                                               <  3:352171/151‑1 (MQ=255)
                                                               ggTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                                                               <  3:37962/151‑1 (MQ=255)
                                                                 ttttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgcg                                                                             >  2:981833/1‑151 (MQ=255)
                                                                 ttttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgcg                                                                             >  1:230036/1‑151 (MQ=255)
                                                                 ttttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgcg                                                                             >  3:235592/1‑151 (MQ=255)
                                                                 ttttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcg                                                                               <  2:509299/149‑1 (MQ=255)
                                                                  tttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAAcgcgcg                                                                             <  4:443791/150‑1 (MQ=255)
                                                                   ttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCgg                                                                            <  3:347746/150‑1 (MQ=255)
                                                                   ttGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGt                                                                           >  2:563210/1‑151 (MQ=255)
                                                                    tGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTg                                                                          >  1:594209/1‑151 (MQ=255)
                                                                      tAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCt                                                                        >  2:121083/1‑151 (MQ=255)
                                                                      tAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCt                                                                        >  3:227703/1‑151 (MQ=255)
                                                                       aGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTg                                                                       <  4:115499/151‑1 (MQ=255)
                                                                        gATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGa                                                                      <  2:643327/151‑1 (MQ=255)
                                                                         atatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGAt                                                                     <  1:479608/151‑1 (MQ=255)
                                                                             ccGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgc                                                                  <  2:543578/150‑1 (MQ=255)
                                                                              cGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcgc                                                                >  2:755509/1‑151 (MQ=255)
                                                                              cGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcgc                                                                >  1:1090704/1‑151 (MQ=255)
                                                                                 aTTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcgcgc                                                              >  1:351647/1‑150 (MQ=255)
                                                                                 aTTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGGTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTgcgcgc                                                              >  3:69270/1‑150 (MQ=255)
                                                                                  ttGTGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGCTATCCGGCACCTAAACGCGCGGTGCTGATTgcgcgc                                                              >  2:1217508/1‑149 (MQ=255)
                                                                                  ttGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCa                                                             >  2:1176562/1‑150 (MQ=255)
                                                                                    gCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCaa                                                            <  2:599604/149‑1 (MQ=255)
                                                                                     cGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGcc                                                         >  2:1253434/1‑151 (MQ=255)
                                                                                       gATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGt                                                       >  2:1280370/1‑151 (MQ=255)
                                                                                        aTGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGt                                                       >  2:636643/1‑150 (MQ=255)
                                                                                           tgAGCGTTACCCCCACAGAGAACCAGGGACGCACCGAATGTATGGTTACCGAATCGCCGGCGGATTTCCCCGACCCACATGATCCGGGTAAAACGGTGAAAGGTTATCCGGCACCTAACCGCGCGGTGCTGATTGCGCGCAAGCCGTaaa                                                    <  1:982026/150‑1 (MQ=255)
                                                                                             aGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGt                                                 >  1:486735/1‑151 (MQ=255)
                                                                                              gCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTCCCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGTTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAg                                                   <  3:81692/148‑1 (MQ=255)
                                                                                              gCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGt                                                 <  3:313173/150‑1 (MQ=255)
                                                                                                gTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTg                                              >  1:1278743/1‑151 (MQ=255)
                                                                                                     caccacAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTAtt                                         <  4:283250/151‑1 (MQ=255)
                                                                                                      accacAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTAtt                                         >  1:854656/1‑150 (MQ=255)
                                                                                                       ccacAGAAGAGCAGCGACGCCCCGAATGGATGGTTACCGAATCGCCGGCCGATTCCCTCGACCCACATGTTCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTAc                                       <  1:996367/151‑1 (MQ=255)
                                                                                                       ccacAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTa                                        <  1:878242/150‑1 (MQ=255)
                                                                                                           agaagaGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGc                                    <  2:547757/150‑1 (MQ=255)
                                                                                                           agaagaGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGc                                    <  1:421441/150‑1 (MQ=255)
                                                                                                             aagaGCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCgg                                 >  2:101923/1‑151 (MQ=255)
                                                                                                              agagCAGCGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGACAGGTATTACTGCCg                                  >  3:276833/1‑149 (MQ=255)
                                                                                                                agcagcGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATg                              <  1:721568/151‑1 (MQ=255)
                                                                                                                 gcagcGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATg                              <  4:113202/150‑1 (MQ=255)
                                                                                                                  cagcGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATgcg                            <  4:39619/151‑1 (MQ=255)
                                                                                                                   agcGACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATgcg                            <  1:970838/150‑1 (MQ=255)
                                                                                                                    gcgcCGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGTTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATgcgg                           <  3:103784/150‑1 (MQ=255)
                                                                                                                      gACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATgcggcg                         <  2:486649/150‑1 (MQ=255)
                                                                                                                      gACGCACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGAGCTGATTGCGCGCAAGCCGTAAAGGTCTGGCATTACTGCCGGATgcggcg                         >  2:1000277/1‑150 (MQ=255)
                                                                                                                          cACCGAATGGATGGTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCTCCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGaa                     <  2:42783/150‑1 (MQ=255)
                                                                                                                                 tggatggTTACCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTAt             >  1:1158157/1‑151 (MQ=255)
                                                                                                                                  ggatggTTCCCGAATCGCCGGCCGATTTCCTCGACCCACATGACCCGGGTAAAACGGTGGAAGGTTATCCGGCCCCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTAt             <  1:246827/150‑1 (MQ=255)
                                                                                                                                     tggTTACCGAATCGCCGGCCGATTTCCCCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCg          <  1:941649/150‑1 (MQ=255)
                                                                                                                                        ttcccgatgcGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCgg         <  1:95157/139‑1 (MQ=255)
                                                                                                                                          aCCGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc    >  2:1005099/1‑151 (MQ=255)
                                                                                                                                           ccGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa   <  1:684833/151‑1 (MQ=255)
                                                                                                                                           ccGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTACCCGGCACCAAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa   <  2:241069/151‑1 (MQ=255)
                                                                                                                                            cGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa   >  3:101362/1‑150 (MQ=255)
                                                                                                                                            cGAATCGCCGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa   >  2:201350/1‑150 (MQ=255)
                                                                                                                                            cGAATCGCCGGCCGATTTCCCCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaa  <  2:1124384/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CAAATGCGTAATGTCTATTTCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAATCGCTGGCCGATTTCCTCGACCCACATGATCCGGGTAAAACGGTGGAAGGTTATCCGGCACCTAAACGCGCGGTGCTGATTGCGCGCAAGCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAA  >  NC_000913/1954174‑1954464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: