Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,222,366 | T→C | V61A (GTG→GCG) | dld → | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,222,366 | 0 | T | C | 94.4% | 228.5 / NA | 92 | V61A (GTG→GCG) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (29/56); minor base G (5/0); total (36/56) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.33e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTC > NC_000913/2222219‑2222512 | ttttGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGgcgc < 1:210861/150‑1 (MQ=255) tgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCCGGAAga > 4:20524/1‑149 (MQ=255) tgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCCGGAAGcc > 2:42891/1‑151 (MQ=255) aaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGAAAGcc > 1:241298/1‑149 (MQ=255) aCTTGCTCGTCTGGTGGGTTCTTCGCACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAG‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGc < 1:49050/151‑1 (MQ=255) aCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGGTGAAAGGCt > 2:392732/1‑149 (MQ=255) tGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCg < 2:2088/149‑1 (MQ=255) tGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCg < 2:84666/149‑1 (MQ=255) gCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTc < 2:897677/150‑1 (MQ=255) gCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCa < 1:1225658/151‑1 (MQ=255) ggtggGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGAc < 1:466795/151‑1 (MQ=255) ggtggGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCG‑TTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACa < 2:381828/151‑1 (MQ=255) cacaCCTGCTCCCCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTc < 2:986122/150‑1 (MQ=255) acacCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGAGCTGATAGACACCGTCACCGCCGCCCAAaatattt > 4:386009/1‑148 (MQ=255) cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGa < 1:1253852/151‑1 (MQ=255) cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCCGCGTCACCGCCCGCAAAaatatt > 2:1186118/1‑147 (MQ=255) cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGTCCCCCCCGCCAAAattatttt > 2:1235998/1‑147 (MQ=255) cacACTGATCCCGCAAAAACGGCCC‑TTATCCGCAAGGGCTGCCGTCCTGGAAGGGTGGACGCGCGGGCTGTCCTTTTCCCTGACACACGTCTATAATTGTGGCGGCCTCGGAAACCCTGCGTCACCGCCGACAAAATTATTCTGATGCAgg < 2:673520/149‑1 (MQ=255) cACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGAAAACCTGCGGCACCGCCGACAAAATTATTCTGATGCAAgcc > 4:287579/1‑151 (MQ=255) cACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCGGGCTTTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATg < 4:195714/145‑1 (MQ=255) aTCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCaa < 1:143926/151‑1 (MQ=255) aTCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCa < 2:1251038/150‑1 (MQ=255) tCCCTCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCCGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgccgccca > 1:21432/1‑148 (MQ=255) cccGAAAAAACGGCCCGCTAT‑CGAAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCTTTAAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgc < 1:606372/143‑1 (MQ=255) cGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACa < 1:242254/151‑1 (MQ=255) cAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCCGCGTCACCGCCGACAAAATTATTTTTATGCAGGCCGCCAATa > 2:1267315/1‑147 (MQ=255) aaaaaCGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTTCGTCACCGCCGACAAAAATATTCTGATTCAGGCCGCCAATACAgg > 1:1207621/1‑150 (MQ=255) aaaaaCGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAgg < 1:883546/150‑1 (MQ=255) aaaaaCGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGc < 2:895772/151‑1 (MQ=255) cGGCCCGTTAT‑CGCAAGGGCTTCCGTTCGGGTCAGGGCGACGCGCTGGCTGTCTTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGAc < 2:268113/151‑1 (MQ=255) cGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGGAAACCCGCGTCCCCCCCCCCAAAAATATTTTGATGCAGggcgcccaaa > 3:28849/1‑137 (MQ=255) ggCCCGCTAT‑CGCAAGGTCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAAAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGAcc < 2:360656/151‑1 (MQ=255) cccGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGa < 1:667417/151‑1 (MQ=255) cccGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAAATATTCTGATGCAGGCCGCCAATACAGGCCTGAcc > 1:225609/1‑149 (MQ=255) gCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAGGCCGGCGTCCCCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAgg < 1:988654/151‑1 (MQ=255) cTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGGAAGCCCGCGTCACCGCCGACAAAAATATTCTGATGCAAGCCGCCCATACAGGCCTGACCGaa > 1:113406/1‑148 (MQ=255) tAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAgg < 2:1207600/149‑1 (MQ=255) aT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCCGAAAACCTGCGGCACCCCCGGCAAAATAATTCTCATGGAGGCCGCCCCTACCGGCCCCACCGAAGGGTc > 2:1036518/1‑151 (MQ=255) aT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATc < 1:766020/151‑1 (MQ=255) t‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATc < 1:546887/150‑1 (MQ=255) gCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTTATGCCGGCCGCCCATAAAAGCCTGACCGAAGGATCg > 1:677636/1‑149 (MQ=255) cAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGAc < 1:61329/150‑1 (MQ=255) gggCTTCCGTTCTGGTCGGGGCGACGCGCTGGATGTCGTTTTCCCGGGCTCACTACAAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGcc < 4:203466/150‑1 (MQ=255) ggCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCaa < 2:10741/151‑1 (MQ=255) ggCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTAACCGCCGACAAAATTATTCTGATCCAGGCGGCCAATTCATGCCTGACCGAAGGATCGACGGCaa < 2:358063/151‑1 (MQ=255) gCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCGCAAAGTCCGAGACCCCGCCGACACAATTATTTTTGTGGAGGCCCCCAATACAGGCGAGACCCAAGGaaggcagccca > 2:1104985/1‑140 (MQ=255) ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAAc < 2:320596/150‑1 (MQ=255) ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGGCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGCAGGATCGACGCCAAAc > 1:436167/1‑150 (MQ=255) tCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACg < 1:892533/150‑1 (MQ=255) cctttCTGGTCAGGGCGCCGCGCGGGCTGTCGTTTTCCCTGGCGCACTACAAGATTTGTGGGGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGt < 2:754106/148‑1 (MQ=255) ccGTTCTGGTCAGGGCGCCGCGCTGGCTGTCGTTTTCCCGGGCTCACTACAAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCaaa < 2:348074/147‑1 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACg < 2:872231/149‑1 (MQ=255) ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGt < 2:765491/151‑1 (MQ=255) cGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGGCGAAAACCCGCGTCACCCCCGACAAAAATATTCTGATGCAGGCCCCCCATAAAGGACCGACCCAAGGGTAGACGcc > 2:6055/1‑143 (MQ=255) ttCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTa < 2:898487/149‑1 (MQ=255) ggTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAAc < 4:184454/141‑1 (MQ=255) ggTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGAtt < 1:424844/151‑1 (MQ=255) cAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGAAAGCCTGCGTCCCCGCCCACAAAAATATTTTGTTGCAGGCCGCCCATACAGGCCTGACCCAAGGATCGACGCCAAACGGTAACGAtt > 3:282026/1‑148 (MQ=255) gggCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGAt < 1:661839/151‑1 (MQ=255) tgACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTTCGTCACCGCCGGCAAAAATATTTTGATGCCGGCCGCCCATACAGGCCTGGCCGGCGGAACGACGCCAAACGGGAACGATTATGAt > 2:514711/2‑148 (MQ=255) gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCCGGAAACCTGCGGCACCGCCGGCAAAAAAATTTTGATGCAGGCCGCCAATACAAGCCTGACCGAAGGATCGACGCCAAAAGGGTAAGATtattataggg > 3:173023/1‑147 (MQ=255) gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATcgc < 4:297536/150‑1 (MQ=255) gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATcg < 2:932493/149‑1 (MQ=255) aCGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGGAAGCCTGCGTCCCCGCCCACAAAATTATTCTGATGGGGGCCGCCCACACAAGCCCTACCGCAGGAGCGACCCCCAACGGTAACGAGTGTGATc > 4:417325/1‑147 (MQ=255) aCGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCCCCCCCCGCAAAATTATTTTTATGCAGGCCGCCCAAACAAGCCCGGCCCGAGGAAGGGCGCCCAACGGGAACGAtt > 2:936912/1‑141 (MQ=255) gcgcTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCCGAAAACCTTCGTTACCGCCCCCAAAAATATTATTATGCATGCCGCCAATACAGGCCCGACCCAAAGAACGACGGCAAACGGGAACgcatttttacgcg > 1:752271/1‑136 (MQ=255) cgcTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGt < 1:167285/151‑1 (MQ=255) ggctgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCg < 1:83678/149‑1 (MQ=255) gctgTCGTTTCCCCTGGCTCACTACTAGAATTGTGACGGGCGCTGAAAGCCTGCGTCCCCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCg < 1:239174/148‑1 (MQ=255) tgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGGTGGAAGACTTCGTCGCCCCCCGAAAAATTAATCTGAAGCAGGCCGCCAAAACAGGGCCGACCGAAGGAGCAGCGACAAAAGGTAACAATTATTATAGCGGTGTAGTTa > 2:627070/1‑149 (MQ=255) cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCCATACAGGGCTGACCGAAGGGTCGACGCCCAAAGGGAACGAATATTATTGCGATGTCGTTatca > 2:1031926/1‑149 (MQ=255) cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTatcat < 4:418633/150‑1 (MQ=255) cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTatcat < 1:903856/150‑1 (MQ=255) cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTatc < 1:439301/148‑1 (MQ=255) gTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTAtcatca < 1:940415/151‑1 (MQ=255) tttCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAg < 2:982156/150‑1 (MQ=255) tttCCCTGGCTCACTACTAGAATTGTGGCGGGCCCTGAAAACCCTCCTCCCCCCCCACAAAATTATTTTGGTGGAAGCCGCCAATAAACGGCTGAACCCAGGGACGACCCCAAACGGCAACGATTATTAGCCCGATGGCGGTatcat > 2:1123384/1‑147 (MQ=255) ttCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGGCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGGAGGATCGACGCCCAACGGTAACGATTATGATCGCGATGTCGTTTTCATCAGc > 1:455053/1‑150 (MQ=255) tCCCTGGCTCACTACTAGAATTGTGGCGGGCGCGGAAAACCCGCGTCACCGCCGCCAAAAATATTCTGGTGCAGGCCCCCAATACAGGCCTGACCGAAGGGTTCACGCCCAAAGGGAACGAATATTATCGCGGTGTCGTTATCATCAGCAc > 1:599409/1‑151 (MQ=255) cccTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGGTCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCAcc > 1:726528/1‑151 (MQ=255) ccTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCAccc < 3:309361/151‑1 (MQ=255) tGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCCGCGCCCCCGCCGACAAAAATATTATGATGTAGGCCGCCAATACAAGCCCGACCGCAGGGACGACGCCAAACGGGAACGAATATAATCGCGATGTCGTTATCATCAACAcc > 4:14255/1‑148 (MQ=255) ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt < 1:359014/149‑1 (MQ=255) ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt < 2:582669/149‑1 (MQ=255) ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAACTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt < 1:357912/149‑1 (MQ=255) cTCACTACTAGAATTGTGGCGGGCGCTGAAAACCCGCGTCACCCCCGCCAAAAATATTTTTATGCAGGCCGCCCATACAGGGCTGACCGAAGGAACGACGCCAAACGGGAACGATTATGATCGCGGTGTCGTTATAATCAGCACCCCGc > 2:775021/1‑149 (MQ=255) ctactaGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGtct < 1:277234/149‑1 (MQ=255) actaGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGAc < 2:245742/151‑1 (MQ=255) aGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACa < 1:301594/149‑1 (MQ=255) aaTTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAg > 1:444752/1‑149 (MQ=255) aTTGTGGCGGGCGCTGGAAGCCCGCGGCACCCCCCCCAAAAATATTTTGATGCAGGCCGCCCAAACAGGCCCGGCCGAAGGATCGACGCCAAACGGTAACGATTATGATTGCGATGTCGTTTTCATCCACACCCCGCCTCCCCACaa > 3:231739/1‑147 (MQ=255) ttGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCAAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTg < 1:1176156/150‑1 (MQ=255) cGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTc > 1:1103341/1‑151 (MQ=255) | TTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAT‑CGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTC > NC_000913/2222219‑2222512 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |