Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,222,366 T→C V61A (GTG→GCG)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,222,3660TC94.4% 228.5 / NA 92V61A (GTG→GCG) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base C (29/56);  minor base G (5/0);  total (36/56)
Fisher's exact test for biased strand distribution p-value = 6.33e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTC  >  NC_000913/2222219‑2222512
                                                                                                                                                    |                                                                                                                                                  
ttttGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGgcgc                                                                                                                                                  <  1:210861/150‑1 (MQ=255)
       tgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCCGGAAga                                                                                                                                           >  4:20524/1‑149 (MQ=255)
       tgaaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCCGGAAGcc                                                                                                                                          >  2:42891/1‑151 (MQ=255)
         aaCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGAAAGcc                                                                                                                                          >  1:241298/1‑149 (MQ=255)
          aCTTGCTCGTCTGGTGGGTTCTTCGCACCTGCTCACCGATCCCGCAAAAACGGCCCGCTAGCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGc                                                                                                                                       <  1:49050/151‑1 (MQ=255)
          aCTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGGTGAAAGGCt                                                                                                                                         >  2:392732/1‑149 (MQ=255)
             tGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCg                                                                                                                                      <  2:2088/149‑1 (MQ=255)
             tGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCg                                                                                                                                      <  2:84666/149‑1 (MQ=255)
              gCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTc                                                                                                                                    <  2:897677/150‑1 (MQ=255)
              gCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCa                                                                                                                                   <  1:1225658/151‑1 (MQ=255)
                      ggtggGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGAc                                                                                                                           <  1:466795/151‑1 (MQ=255)
                      ggtggGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACa                                                                                                                          <  2:381828/151‑1 (MQ=255)
                                 cacaCCTGCTCCCCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTc                                                                                                                 <  2:986122/150‑1 (MQ=255)
                                  acacCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGAGCTGATAGACACCGTCACCGCCGCCCAAaatattt                                                                                                                 >  4:386009/1‑148 (MQ=255)
                                   cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGa                                                                                                              <  1:1253852/151‑1 (MQ=255)
                                   cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCCGCGTCACCGCCCGCAAAaatatt                                                                                                                  >  2:1186118/1‑147 (MQ=255)
                                   cacCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGTCCCCCCCGCCAAAattatttt                                                                                                                >  2:1235998/1‑147 (MQ=255)
                                         cacACTGATCCCGCAAAAACGGCCCTTATCCGCAAGGGCTGCCGTCCTGGAAGGGTGGACGCGCGGGCTGTCCTTTTCCCTGACACACGTCTATAATTGTGGCGGCCTCGGAAACCCTGCGTCACCGCCGACAAAATTATTCTGATGCAgg                                                                                                        <  2:673520/149‑1 (MQ=255)
                                           cACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGAAAACCTGCGGCACCGCCGACAAAATTATTCTGATGCAAgcc                                                                                                      >  4:287579/1‑151 (MQ=255)
                                           cACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCGGGCTTTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATg                                                                                                            <  4:195714/145‑1 (MQ=255)
                                                aTCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCaa                                                                                                 <  1:143926/151‑1 (MQ=255)
                                                aTCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCa                                                                                                  <  2:1251038/150‑1 (MQ=255)
                                                 tCCCTCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCCGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgccgccca                                                                                                 >  1:21432/1‑148 (MQ=255)
                                                  cccGAAAAAACGGCCCGCTATCGAAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCTTTAAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGgc                                                                                                       <  1:606372/143‑1 (MQ=255)
                                                    cGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACa                                                                                             <  1:242254/151‑1 (MQ=255)
                                                      cAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCCGCGTCACCGCCGACAAAATTATTTTTATGCAGGCCGCCAATa                                                                                               >  2:1267315/1‑147 (MQ=255)
                                                       aaaaaCGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTTCGTCACCGCCGACAAAAATATTCTGATTCAGGCCGCCAATACAgg                                                                                           >  1:1207621/1‑150 (MQ=255)
                                                       aaaaaCGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAgg                                                                                           <  1:883546/150‑1 (MQ=255)
                                                       aaaaaCGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGc                                                                                          <  2:895772/151‑1 (MQ=255)
                                                            cGGCCCGTTATCGCAAGGGCTTCCGTTCGGGTCAGGGCGACGCGCTGGCTGTCTTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGAc                                                                                     <  2:268113/151‑1 (MQ=255)
                                                            cGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGGAAACCCGCGTCCCCCCCCCCAAAAATATTTTGATGCAGggcgcccaaa                                                                                               >  3:28849/1‑137 (MQ=255)
                                                             ggCCCGCTATCGCAAGGTCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAAAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGAcc                                                                                    <  2:360656/151‑1 (MQ=255)
                                                               cccGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGa                                                                                  <  1:667417/151‑1 (MQ=255)
                                                               cccGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAAATATTCTGATGCAGGCCGCCAATACAGGCCTGAcc                                                                                    >  1:225609/1‑149 (MQ=255)
                                                                  gCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAGGCCGGCGTCCCCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAgg                                                                               <  1:988654/151‑1 (MQ=255)
                                                                   cTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGGAAGCCCGCGTCACCGCCGACAAAAATATTCTGATGCAAGCCGCCCATACAGGCCTGACCGaa                                                                                 >  1:113406/1‑148 (MQ=255)
                                                                    tATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAgg                                                                               <  2:1207600/149‑1 (MQ=255)
                                                                     aTCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCCGAAAACCTGCGGCACCCCCGGCAAAATAATTCTCATGGAGGCCGCCCCTACCGGCCCCACCGAAGGGTc                                                                            >  2:1036518/1‑151 (MQ=255)
                                                                     aTCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATc                                                                            <  1:766020/151‑1 (MQ=255)
                                                                      tCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATc                                                                            <  1:546887/150‑1 (MQ=255)
                                                                         gCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTTATGCCGGCCGCCCATAAAAGCCTGACCGAAGGATCg                                                                           >  1:677636/1‑149 (MQ=255)
                                                                          cAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGAc                                                                         <  1:61329/150‑1 (MQ=255)
                                                                             gggCTTCCGTTCTGGTCGGGGCGACGCGCTGGATGTCGTTTTCCCGGGCTCACTACAAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGcc                                                                      <  4:203466/150‑1 (MQ=255)
                                                                              ggCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCaa                                                                    <  2:10741/151‑1 (MQ=255)
                                                                              ggCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTAACCGCCGACAAAATTATTCTGATCCAGGCGGCCAATTCATGCCTGACCGAAGGATCGACGGCaa                                                                    <  2:358063/151‑1 (MQ=255)
                                                                               gCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCGCAAAGTCCGAGACCCCGCCGACACAATTATTTTTGTGGAGGCCCCCAATACAGGCGAGACCCAAGGaaggcagccca                                                                    >  2:1104985/1‑140 (MQ=255)
                                                                                 ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAAc                                                                  <  2:320596/150‑1 (MQ=255)
                                                                                 ttCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGGCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGCAGGATCGACGCCAAAc                                                                  >  1:436167/1‑150 (MQ=255)
                                                                                  tCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACg                                                                 <  1:892533/150‑1 (MQ=255)
                                                                                   cctttCTGGTCAGGGCGCCGCGCGGGCTGTCGTTTTCCCTGGCGCACTACAAGATTTGTGGGGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGt                                                               <  2:754106/148‑1 (MQ=255)
                                                                                   ccGTTCTGGTCAGGGCGCCGCGCTGGCTGTCGTTTTCCCGGGCTCACTACAAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCaaa                                                                   <  2:348074/147‑1 (MQ=255)
                                                                                   ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACg                                                                 <  2:872231/149‑1 (MQ=255)
                                                                                   ccGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGt                                                               <  2:765491/151‑1 (MQ=255)
                                                                                    cGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGGCGAAAACCCGCGTCACCCCCGACAAAAATATTCTGATGCAGGCCCCCCATAAAGGACCGACCCAAGGGTAGACGcc                                                                      >  2:6055/1‑143 (MQ=255)
                                                                                      ttCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTa                                                              <  2:898487/149‑1 (MQ=255)
                                                                                          ggTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAAc                                                                  <  4:184454/141‑1 (MQ=255)
                                                                                          ggTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGAtt                                                        <  1:424844/151‑1 (MQ=255)
                                                                                             cAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCTGAAAGCCTGCGTCCCCGCCCACAAAAATATTTTGTTGCAGGCCGCCCATACAGGCCTGACCCAAGGATCGACGCCAAACGGTAACGAtt                                                        >  3:282026/1‑148 (MQ=255)
                                                                                               gggCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGAt                                                   <  1:661839/151‑1 (MQ=255)
                                                                                                  tgACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTTCGTCACCGCCGGCAAAAATATTTTGATGCCGGCCGCCCATACAGGCCTGGCCGGCGGAACGACGCCAAACGGGAACGATTATGAt                                                   >  2:514711/2‑148 (MQ=255)
                                                                                                   gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGGGCCGGAAACCTGCGGCACCGCCGGCAAAAAAATTTTGATGCAGGCCGCCAATACAAGCCTGACCGAAGGATCGACGCCAAAAGGGTAAGATtattataggg                                               >  3:173023/1‑147 (MQ=255)
                                                                                                   gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATcgc                                                <  4:297536/150‑1 (MQ=255)
                                                                                                   gACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATcg                                                 <  2:932493/149‑1 (MQ=255)
                                                                                                    aCGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGGAAGCCTGCGTCCCCGCCCACAAAATTATTCTGATGGGGGCCGCCCACACAAGCCCTACCGCAGGAGCGACCCCCAACGGTAACGAGTGTGATc                                                  >  4:417325/1‑147 (MQ=255)
                                                                                                    aCGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCCCCCCCCGCAAAATTATTTTTATGCAGGCCGCCCAAACAAGCCCGGCCCGAGGAAGGGCGCCCAACGGGAACGAtt                                                        >  2:936912/1‑141 (MQ=255)
                                                                                                      gcgcTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCCGAAAACCTTCGTTACCGCCCCCAAAAATATTATTATGCATGCCGCCAATACAGGCCCGACCCAAAGAACGACGGCAAACGGGAACgcatttttacgcg                                               >  1:752271/1‑136 (MQ=255)
                                                                                                       cgcTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGt                                           <  1:167285/151‑1 (MQ=255)
                                                                                                           ggctgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCg                                         <  1:83678/149‑1 (MQ=255)
                                                                                                            gctgTCGTTTCCCCTGGCTCACTACTAGAATTGTGACGGGCGCTGAAAGCCTGCGTCCCCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCg                                         <  1:239174/148‑1 (MQ=255)
                                                                                                              tgTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGGTGGAAGACTTCGTCGCCCCCCGAAAAATTAATCTGAAGCAGGCCGCCAAAACAGGGCCGACCGAAGGAGCAGCGACAAAAGGTAACAATTATTATAGCGGTGTAGTTa                                      >  2:627070/1‑149 (MQ=255)
                                                                                                                 cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCCATACAGGGCTGACCGAAGGGTCGACGCCCAAAGGGAACGAATATTATTGCGATGTCGTTatca                                   >  2:1031926/1‑149 (MQ=255)
                                                                                                                 cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTatcat                                  <  4:418633/150‑1 (MQ=255)
                                                                                                                 cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTatcat                                  <  1:903856/150‑1 (MQ=255)
                                                                                                                 cGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTatc                                    <  1:439301/148‑1 (MQ=255)
                                                                                                                  gTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTAtcatca                                <  1:940415/151‑1 (MQ=255)
                                                                                                                    tttCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAg                               <  2:982156/150‑1 (MQ=255)
                                                                                                                    tttCCCTGGCTCACTACTAGAATTGTGGCGGGCCCTGAAAACCCTCCTCCCCCCCCACAAAATTATTTTGGTGGAAGCCGCCAATAAACGGCTGAACCCAGGGACGACCCCAAACGGCAACGATTATTAGCCCGATGGCGGTatcat                                  >  2:1123384/1‑147 (MQ=255)
                                                                                                                     ttCCCTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGGCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGGAGGATCGACGCCCAACGGTAACGATTATGATCGCGATGTCGTTTTCATCAGc                              >  1:455053/1‑150 (MQ=255)
                                                                                                                      tCCCTGGCTCACTACTAGAATTGTGGCGGGCGCGGAAAACCCGCGTCACCGCCGCCAAAAATATTCTGGTGCAGGCCCCCAATACAGGCCTGACCGAAGGGTTCACGCCCAAAGGGAACGAATATTATCGCGGTGTCGTTATCATCAGCAc                            >  1:599409/1‑151 (MQ=255)
                                                                                                                       cccTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGGTCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCAcc                           >  1:726528/1‑151 (MQ=255)
                                                                                                                        ccTGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCAccc                          <  3:309361/151‑1 (MQ=255)
                                                                                                                          tGGCTCACTACTAGAATTGTGGCGGGCGCTGAAAACCCGCGCCCCCGCCGACAAAAATATTATGATGTAGGCCGCCAATACAAGCCCGACCGCAGGGACGACGCCAAACGGGAACGAATATAATCGCGATGTCGTTATCATCAACAcc                           >  4:14255/1‑148 (MQ=255)
                                                                                                                           ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt                         <  1:359014/149‑1 (MQ=255)
                                                                                                                           ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt                         <  2:582669/149‑1 (MQ=255)
                                                                                                                           ggCTCACTACTAGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAACTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCt                         <  1:357912/149‑1 (MQ=255)
                                                                                                                             cTCACTACTAGAATTGTGGCGGGCGCTGAAAACCCGCGTCACCCCCGCCAAAAATATTTTTATGCAGGCCGCCCATACAGGGCTGACCGAAGGAACGACGCCAAACGGGAACGATTATGATCGCGGTGTCGTTATAATCAGCACCCCGc                       >  2:775021/1‑149 (MQ=255)
                                                                                                                                 ctactaGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGtct                   <  1:277234/149‑1 (MQ=255)
                                                                                                                                   actaGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGAc               <  2:245742/151‑1 (MQ=255)
                                                                                                                                      aGAATTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACa              <  1:301594/149‑1 (MQ=255)
                                                                                                                                        aaTTGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAg            >  1:444752/1‑149 (MQ=255)
                                                                                                                                         aTTGTGGCGGGCGCTGGAAGCCCGCGGCACCCCCCCCAAAAATATTTTGATGCAGGCCGCCCAAACAGGCCCGGCCGAAGGATCGACGCCAAACGGTAACGATTATGATTGCGATGTCGTTTTCATCCACACCCCGCCTCCCCACaa             >  3:231739/1‑147 (MQ=255)
                                                                                                                                          ttGTGGCGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCAAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTg         <  1:1176156/150‑1 (MQ=255)
                                                                                                                                                cGGGCGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTc  >  1:1103341/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGCCGACAAAATTATTCTGATGCAGGCCGCCAATACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTC  >  NC_000913/2222219‑2222512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: