Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,317,917	T→C	T654A (ACC→GCC)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,317,917	0	T	C	100.0%	355.9 / NA	98	T654A (ACC→GCC)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/50);  total (48/50)

TCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA  >  NC_000913/2317772‑2318064                                                                                                                                                  |                                                                                                                                                    tCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTTCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGcccc                                                                                                                                                  <  2:419847/149‑1 (MQ=255) tCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCcccc                                                                                                                                                 <  1:946311/150‑1 (MQ=255)   cACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTgg                                                                                                                                              <  3:27621/151‑1 (MQ=255)   cACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTgg                                                                                                                                              >  1:236036/1‑151 (MQ=255)      gCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGaa                                                                                                                                            >  2:374077/1‑150 (MQ=255)       ccTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAAt                                                                                                                                          <  2:267688/151‑1 (MQ=255)          tttttCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAAtt                                                                                                                                         <  1:74989/149‑1 (MQ=255)          tttttCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAAtt                                                                                                                                         <  3:357284/149‑1 (MQ=255)          tttttCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTAc                                                                                                                                       >  1:338272/1‑151 (MQ=255)           atttATGCGGGCACTGAGCGCCGAACAACGGAATACGCACGGTAAACTTGCTGCCACTTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGCTTTTCACAAATCGCCAGACCCAGACGGGCCCCCTGGaagt                                                                                                                                          >  2:525301/2‑145 (MQ=255)            tttCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACg                                                                                                                                      <  4:366843/150‑1 (MQ=255)                          gAGCGCCGTACAAGTGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCATTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTcc                                                                                                                       <  2:698920/151‑1 (MQ=255)                           aGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCg                                                                                                                      >  1:1032673/1‑151 (MQ=255)                               ccGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCt                                                                                                                  <  2:1132384/151‑1 (MQ=255)                               ccGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCt                                                                                                                  >  1:920543/1‑151 (MQ=255)                                cGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTg                                                                                                                 <  1:1259519/151‑1 (MQ=255)                                cGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTg                                                                                                                 <  2:456446/151‑1 (MQ=255)                                    cAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGgaag                                                                                                             <  4:380730/151‑1 (MQ=255)                                     aaCGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGgaaga                                                                                                            <  1:340304/151‑1 (MQ=255)                                         gAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAggg                                                                                                        >  1:309860/1‑151 (MQ=255)                                          aaTACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGa                                                                                                       <  2:28418/151‑1 (MQ=255)                                             aCGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATc                                                                                                     >  2:608907/1‑150 (MQ=255)                                             aCGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATc                                                                                                     <  2:180940/150‑1 (MQ=255)                                               gCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTTCGCCCGTTCCGACCTGGAAGAAGTGATCaaa                                                                                                  <  4:237866/151‑1 (MQ=255)                                                cACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAAc                                                                                                 >  1:203111/1‑151 (MQ=255)                                                   ggTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAg                                                                                              <  3:439142/151‑1 (MQ=255)                                                     tAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAgcg                                                                                            >  2:835655/1‑151 (MQ=255)                                                      aaaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCTGACCTGGAAGAAGGGATCAAACAAgcgc                                                                                           >  3:94228/1‑151 (MQ=255)                                                       aaCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGca                                                                                          <  4:355831/151‑1 (MQ=255)                                                           ggCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGcacc                                                                                        <  1:1071269/149‑1 (MQ=255)                                                             cTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActt                                                                                    >  1:659318/1‑151 (MQ=255)                                                               gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtct                                                                                  <  2:142370/151‑1 (MQ=255)                                                               gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtct                                                                                  >  1:1211889/1‑151 (MQ=255)                                                               gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtc                                                                                   <  4:86755/150‑1 (MQ=255)                                                                cccATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtctt                                                                                 >  2:215492/1‑151 (MQ=255)                                                                  cATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTc                                                                               <  2:189713/151‑1 (MQ=255)                                                                  cATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTc                                                                               <  1:402883/151‑1 (MQ=255)                                                                       ccGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTGCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCgg                                                                          <  2:472514/151‑1 (MQ=255)                                                                       ccGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCgg                                                                          <  1:444218/151‑1 (MQ=255)                                                                        cGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt                                                                         <  1:1186210/151‑1 (MQ=255)                                                                         ggTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt                                                                         >  2:669427/1‑150 (MQ=255)                                                                         ggTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt                                                                         >  2:863699/1‑150 (MQ=255)                                                                           ttCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATc                                                                      >  2:436758/1‑151 (MQ=255)                                                                            tCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATcc                                                                     >  2:62110/1‑151 (MQ=255)                                                                                aaTCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCAc                                                                 >  2:68792/1‑151 (MQ=255)                                                                                aaTCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCAc                                                                 >  2:1198182/1‑151 (MQ=255)                                                                                aaTCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCAc                                                                 >  2:1078333/1‑151 (MQ=255)                                                                                  tCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGc                                                               >  3:234928/1‑151 (MQ=255)                                                                                    tACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCg                                                             >  1:763652/1‑151 (MQ=255)                                                                                     aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGc                                                               <  2:961208/148‑1 (MQ=255)                                                                                       cGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGt                                                           >  1:155965/1‑150 (MQ=255)                                                                                       cGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGt                                                           >  1:1236580/1‑150 (MQ=255)                                                                                         agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATc                                                        <  4:327262/151‑1 (MQ=255)                                                                                         agaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATc                                                        >  1:1124403/1‑151 (MQ=255)                                                                                          gaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGCCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGAATCCCCACGCCGGTATc                                                        >  4:213556/1‑150 (MQ=255)                                                                                                gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAaca                                                 <  1:911676/151‑1 (MQ=255)                                                                                                     ccATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAt                                            <  2:1182874/151‑1 (MQ=255)                                                                                                     ccATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAt                                            <  4:360514/151‑1 (MQ=255)                                                                                                     ccATCATGCTGATAAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTCCGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAACGCACCACTTCTTTCGCCGGCATCCCCACGCCGGTATCGCGAACACGGAt                                            <  3:6172/151‑1 (MQ=255)                                                                                                      catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGAt                                            >  1:370196/1‑150 (MQ=255)                                                                                                      catcatGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATa                                           <  2:18772/151‑1 (MQ=255)                                                                                                          atGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATag                                          >  2:1181603/1‑148 (MQ=255)                                                                                                            gCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATagagag                                      <  1:677764/150‑1 (MQ=255)                                                                                                              tGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGAt                                    >  1:1174077/1‑150 (MQ=255)                                                                                                                aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAt                                 >  3:438894/1‑151 (MQ=255)                                                                                                                aTCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAAt                                 >  4:354832/1‑151 (MQ=255)                                                                                                                   aGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCg                               <  1:179419/150‑1 (MQ=255)                                                                                                                    gTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGcc                             <  2:15209/151‑1 (MQ=255)                                                                                                                     tttttCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGcc                             >  1:338204/1‑150 (MQ=255)                                                                                                                      ttttCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCAt                           >  4:219550/1‑151 (MQ=255)                                                                                                                         tCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATcc                         <  2:378196/150‑1 (MQ=255)                                                                                                                          cacaAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgc                       >  2:947243/1‑151 (MQ=255)                                                                                                                          cacaAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcg                        <  2:1243654/150‑1 (MQ=255)                                                                                                                            caATTCGCCATACCCAGACCGGCCCCCTGGAAATTCCGCTGTCCGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcg                      <  1:730508/150‑1 (MQ=255)                                                                                                                            caAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgc                     >  4:393189/1‑151 (MQ=255)                                                                                                                            caAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcg                      <  1:259296/150‑1 (MQ=255)                                                                                                                             aaaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCTCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg                    <  3:216936/151‑1 (MQ=255)                                                                                                                             aaaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg                    <  2:504424/151‑1 (MQ=255)                                                                                                                             aaaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg                    >  1:674793/1‑151 (MQ=255)                                                                                                                             aaaTCGCCAGACCCAGACCGACCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg                    >  4:284258/1‑151 (MQ=255)                                                                                                                              aaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg                    >  3:199667/1‑150 (MQ=255)                                                                                                                              aaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg                    >  1:554985/1‑150 (MQ=255)                                                                                                                                 cGCCAGACCCAGGCCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGaa                  <  1:505039/149‑1 (MQ=255)                                                                                                                                 cGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAAc                 >  1:574449/1‑150 (MQ=255)                                                                                                                                 cGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACa                <  1:703019/151‑1 (MQ=255)                                                                                                                                  gCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACAt               >  1:765595/1‑151 (MQ=255)                                                                                                                                  gCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACAt               >  2:243888/1‑151 (MQ=255)                                                                                                                                    cagacccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGc             >  3:360882/1‑151 (MQ=255)                                                                                                                                      gacccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCaa           <  4:272678/151‑1 (MQ=255)                                                                                                                                       acccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaa          <  1:1120480/151‑1 (MQ=255)                                                                                                                                        cccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaaa         <  2:1265490/151‑1 (MQ=255)                                                                                                                                         ccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAAc        >  2:1213121/1‑151 (MQ=255)                                                                                                                                          cagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACt       >  1:978591/1‑151 (MQ=255)                                                                                                                                           agaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACta      <  1:616142/151‑1 (MQ=255)                                                                                                                                             accGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCTAAACtat     <  2:1210788/150‑1 (MQ=255)                                                                                                                                              ccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtata    <  2:964187/150‑1 (MQ=255)                                                                                                                                              ccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATAc   >  2:169583/1‑151 (MQ=255)                                                                                                                                               cGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATAc   >  2:771060/1‑150 (MQ=255)                                                                                                                                               cGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACa  <  1:392480/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TCCACGCCTTTTTTCTGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACA  >  NC_000913/2317772‑2318064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑