Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,405,949 G→A A231A (GCC→GCT lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,405,9490GA99.1% 416.7 / NA 113A231A (GCC→GCTlrhAtranscriptional repressor of flagellar, motility and chemotaxis genes
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (59/53);  minor base C (1/0);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTT  >  NC_000913/2405805‑2406095
                                                                                                                                                |                                                                                                                                                  
cACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCttt                                                                                                                                               <  2:252172/150‑1 (MQ=255)
cACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAAACCAGCttt                                                                                                                                               >  2:1237857/1‑150 (MQ=255)
 aCCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGGAAACCATCTACTTCACTCAGCACACGCAGGTCCGGGCCCATCATCTCAACCGCCCTTGCCGTCACGCCAACACCAGCttt                                                                                                                                               >  1:681977/1‑149 (MQ=255)
  ccTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCa                                                                                                                                             <  2:181545/150‑1 (MQ=255)
  ccTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCAc                                                                                                                                            <  2:501601/151‑1 (MQ=255)
     gggCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTg                                                                                                                                          <  3:79753/150‑1 (MQ=255)
      ggCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGcc                                                                                                                                        <  1:171219/151‑1 (MQ=255)
        ccAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGc                                                                                                                                      <  1:34708/151‑1 (MQ=255)
          aGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCAc                                                                                                                                    >  4:292618/1‑151 (MQ=255)
           gCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACg                                                                                                                                   >  1:99619/1‑151 (MQ=255)
              cATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAAc                                                                                                                                <  2:203686/151‑1 (MQ=255)
               attattACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCCGGTCCGGGCTCATCATCTCAAACGGCCTTGCCGTCCCGCCAAAATCAGCTTTCACTGCCGCACGAAc                                                                                                                                >  1:816149/1‑150 (MQ=255)
               attattACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAACCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAAc                                                                                                                                >  1:1155932/1‑150 (MQ=255)
                  attaCTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGc                                                                                                                             <  2:761836/150‑1 (MQ=255)
                   ttaCTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGcc                                                                                                                            >  1:937897/1‑150 (MQ=255)
                    taCTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTTACTGCCGCACCAACGGCCgg                                                                                                                          >  2:339791/1‑151 (MQ=255)
                        ggACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGc                                                                                                                      <  4:316496/151‑1 (MQ=255)
                          aCGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCg                                                                                                                     >  1:791107/1‑150 (MQ=255)
                          aCGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGt                                                                                                                    <  2:461762/151‑1 (MQ=255)
                            ggATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCCAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTc                                                                                                                   >  2:68184/1‑150 (MQ=255)
                                cATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGGCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGGAGCGTCGAgg                                                                                                               >  3:118617/1‑150 (MQ=255)
                                 aTAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCCAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAgg                                                                                                               >  2:884977/1‑149 (MQ=255)
                                    gCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAgg                                                                                                               >  2:1001472/1‑146 (MQ=255)
                                    gCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACa                                                                                                          <  3:96022/151‑1 (MQ=255)
                                    gCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAACCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGc                                                                                                              >  1:263810/1‑147 (MQ=255)
                                     cacaGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACa                                                                                                          >  2:1090588/1‑150 (MQ=255)
                                      acaGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACAt                                                                                                         >  2:1031868/1‑150 (MQ=255)
                                       caGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATaa                                                                                                       <  3:232442/151‑1 (MQ=255)
                                        aGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATa                                                                                                        >  4:242878/1‑149 (MQ=255)
                                        aGCAAATATTCGGTATCAGGTAGCGGCGGTAAACCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATa                                                                                                        >  1:108652/1‑149 (MQ=255)
                                           aaaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCCCTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATaa                                                                                                       >  4:336173/1‑147 (MQ=255)
                                           aaaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGcc                                                                                                    >  2:965509/1‑150 (MQ=255)
                                           aaaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGTGGCGACATAAGcc                                                                                                    >  1:1021346/1‑150 (MQ=255)
                                            aaTATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAg                                                                                                  <  1:161921/151‑1 (MQ=255)
                                                ttCGGTATCAGGTAGCGGCGGTAAGACATCTACTCCACTCAGCACACGCAGGTCCGTGCTCCTCATCTCAACCAGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGGCGAAACGACAAAAGCCAGt                                                                                                 >  3:89456/1‑148 (MQ=255)
                                                 tCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGc                                                                                              <  1:617161/150‑1 (MQ=255)
                                                  cGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGcc                                                                                             >  4:189687/1‑150 (MQ=255)
                                                  cGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCa                                                                                            <  2:1203027/151‑1 (MQ=255)
                                                  cGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTAACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCa                                                                                            <  2:522943/151‑1 (MQ=255)
                                                   ggTATCAGGTAGCGGCGGTAAGCCCTCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGcc                                                                                             >  2:755174/1‑149 (MQ=255)
                                                    gTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCCTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACCAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCCTg                                                                                          >  2:837066/1‑151 (MQ=255)
                                                      aTCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATgg                                                                                         <  1:707484/150‑1 (MQ=255)
                                                       tCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGa                                                                                        >  3:54053/1‑150 (MQ=255)
                                                        cAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGaa                                                                                       <  2:930767/150‑1 (MQ=255)
                                                          ggTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAatat                                                                                    <  2:141689/151‑1 (MQ=255)
                                                           gTAGCGGCGGTAAGCCATCTACTCCACTCCGCACACCCAGGGCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCCAGACCCGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACCTAAGCCAGTCGCCACGGGatat                                                                                    >  3:125686/1‑150 (MQ=255)
                                                            tAGCGGCGGTAAGCCCTCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCCTGGAatat                                                                                    >  2:978461/1‑149 (MQ=255)
                                                               cggcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCt                                                                               >  2:412929/1‑151 (MQ=255)
                                                                 gcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCttt                                                                             <  2:347232/151‑1 (MQ=255)
                                                                 gcggTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCttt                                                                             <  3:315592/151‑1 (MQ=255)
                                                                    gTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                                                                          >  1:879528/1‑151 (MQ=255)
                                                                    gTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTttatt                                                                          <  3:410308/151‑1 (MQ=255)
                                                                        gCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGc                                                                      <  4:4357/151‑1 (MQ=255)
                                                                         ccATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCg                                                                     >  2:308783/1‑151 (MQ=255)
                                                                           aTCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGt                                                                    >  2:360637/1‑150 (MQ=255)
                                                                               aCTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCg                                                                  <  2:60222/148‑1 (MQ=255)
                                                                                cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc                                                                >  1:826809/1‑149 (MQ=255)
                                                                                cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc                                                                >  1:958078/1‑149 (MQ=255)
                                                                                cTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGcc                                                                >  1:147566/1‑149 (MQ=255)
                                                                                 tCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGc                                                             >  1:1127647/1‑151 (MQ=255)
                                                                                  ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAg                                                              <  2:970076/149‑1 (MQ=255)
                                                                                  ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGc                                                             <  1:1009262/150‑1 (MQ=255)
                                                                                  ccACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCa                                                            <  2:543380/151‑1 (MQ=255)
                                                                                       cAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCata                                                       <  2:1155722/151‑1 (MQ=255)
                                                                                          cacaCGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATcg                                                    <  2:653455/151‑1 (MQ=255)
                                                                                          cacaCGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATcg                                                    <  1:736886/151‑1 (MQ=255)
                                                                                           acacGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATcgc                                                   >  3:101881/1‑151 (MQ=255)
                                                                                               gCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGaaa                                               <  2:945420/151‑1 (MQ=255)
                                                                                                 aGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCCAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGACAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAAc                                              >  1:918937/1‑150 (MQ=255)
                                                                                                  ggTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACgg                                            >  4:68058/1‑151 (MQ=255)
                                                                                                     ccGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCt                                         >  1:233545/1‑151 (MQ=255)
                                                                                                      cGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTa                                        <  1:1121562/151‑1 (MQ=255)
                                                                                                        ggCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAgg                                      >  2:772928/1‑151 (MQ=255)
                                                                                                           tcatcaTCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatc                                   >  2:55193/1‑151 (MQ=255)
                                                                                                            catcatCTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatca                                  <  1:1123423/151‑1 (MQ=255)
                                                                                                             atcatcTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatcat                                 >  2:123046/1‑151 (MQ=255)
                                                                                                             atcatcTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatcat                                 >  4:119949/1‑151 (MQ=255)
                                                                                                              tcatcTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGatcatc                                >  1:120729/1‑151 (MQ=255)
                                                                                                                atcTCAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAAGGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGATACGGGCTAGGatcatc                                >  1:866405/1‑149 (MQ=255)
                                                                                                                  ctcAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGc                            >  2:1076412/1‑151 (MQ=255)
                                                                                                                   tcAACCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGc                            >  1:1040717/1‑150 (MQ=255)
                                                                                                                     aaCCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCTCCATATCGCGAAACGGGCTAGGATCATCCAg                             <  1:1172339/147‑1 (MQ=255)
                                                                                                                     aaCCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAAt                         >  2:892167/1‑151 (MQ=255)
                                                                                                                      aCCGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATa                        <  3:292614/151‑1 (MQ=255)
                                                                                                                       ccGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATAc                       >  1:974057/1‑151 (MQ=255)
                                                                                                                       ccGGCCTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATAc                       <  1:411398/151‑1 (MQ=255)
                                                                                                                          cccTTGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGCGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAAt                         >  1:1265225/2‑146 (MQ=255)
                                                                                                                             ttGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAgg                 >  2:815098/1‑151 (MQ=255)
                                                                                                                              tGCCGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAagag                  <  2:525140/149‑1 (MQ=255)
                                                                                                                                ccGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGAt              <  3:74861/151‑1 (MQ=255)
                                                                                                                                ccGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGAt              <  1:35552/151‑1 (MQ=255)
                                                                                                                                 cGTCACGCCAAGGCCAGCTTCCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTAGTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGAATCATCCAGCAATACAAGAGGGATc             <  2:895141/151‑1 (MQ=255)
                                                                                                                                 cGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATc             >  3:214181/1‑151 (MQ=255)
                                                                                                                                 cGTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATc             <  3:15888/151‑1 (MQ=255)
                                                                                                                                  gTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCg            <  2:263774/151‑1 (MQ=255)
                                                                                                                                  gTCACGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCg            <  4:114838/151‑1 (MQ=255)
                                                                                                                                     aCGCCAAGACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGc          >  1:1089184/1‑150 (MQ=255)
                                                                                                                                           aGACCAGCTTTCACGGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCtt   <  2:681838/151‑1 (MQ=255)
                                                                                                                                            gACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCttt  <  1:1198718/151‑1 (MQ=255)
                                                                                                                                            gACCAGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCttt  >  1:1227196/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CACCTGGGCCAGCTCATTATTACTGGACGGATCATAGCACAGCAAATATTCGGTATCAGGTAGCGGCGGTAAGCCATCTACTCCACTCAGCACACGCAGGTCCGGGCTCATCATCTCAACCGGCCTTGCCGTCACGCCAAGACCGGCTTTCACTGCCGCACGAACGGCCGGAAGCGTCGAGGCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTT  >  NC_000913/2405805‑2406095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: