Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,747,020 G→A A259V (GCG→GTG)  ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,747,0200GA99.1% 373.6 / NA 109A259V (GCG→GTG) ffhSignal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (50/58);  minor base C (0/1);  total (50/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 114 total aligned reads displayed.

CGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCG  >  NC_000913/2746874‑2747164
                                                                                                                                                  |                                                                                                                                                
cGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGcaccgc                                                                                                                                              >  4:302087/1‑151 (MQ=255)
cGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGcaccgc                                                                                                                                              >  2:397052/1‑151 (MQ=255)
  atatCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCa                                                                                                                                             <  2:779868/150‑1 (MQ=255)
     tCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCaccgcaccgc                                                                                                                                         >  2:454141/1‑151 (MQ=255)
       ttCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCAccgccg                                                                                                                                       >  1:93373/1‑151 (MQ=255)
       ttCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCAccgccg                                                                                                                                       >  2:1059626/1‑151 (MQ=255)
        tCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCaccgcaccgc                                                                                                                                         <  1:695432/148‑1 (MQ=255)
        tCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCaccgcaccgc                                                                                                                                         <  1:1010188/148‑1 (MQ=255)
        tCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCAccgccg                                                                                                                                       <  2:92570/150‑1 (MQ=255)
        tCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACcgccgc                                                                                                                                      >  1:1169375/1‑151 (MQ=255)
         cGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCcgcg                                                                                                                                     <  3:413714/151‑1 (MQ=255)
           aTCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCggg                                                                                                                                   <  1:559248/151‑1 (MQ=255)
             cAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGc                                                                                                                                  <  2:299625/150‑1 (MQ=255)
              aGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACTCCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCAt                                                                                                                                <  2:745313/151‑1 (MQ=255)
              aGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCAt                                                                                                                                >  2:958140/1‑151 (MQ=255)
               gCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATc                                                                                                                               >  1:1036611/1‑151 (MQ=255)
                cGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATc                                                                                                                               >  2:680655/1‑150 (MQ=255)
                  aCAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGcc                                                                                                                            >  2:400299/1‑151 (MQ=255)
                    aGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCg                                                                                                                           >  2:319098/1‑150 (MQ=255)
                      tACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGCGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTcc                                                                                                                        >  2:354993/1‑151 (MQ=255)
                      tACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTcc                                                                                                                        >  3:303485/1‑151 (MQ=255)
                       aCGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTcc                                                                                                                        >  1:861279/1‑150 (MQ=255)
                           cGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACttt                                                                                                                   >  2:560929/1‑151 (MQ=255)
                           cGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACttt                                                                                                                   <  2:1081588/151‑1 (MQ=255)
                            gCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGGCGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTg                                                                                                                  >  3:157615/1‑151 (MQ=255)
                            gCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTg                                                                                                                  >  2:1095619/1‑151 (MQ=255)
                               taTGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTc                                                                                                               >  2:683737/2‑151 (MQ=255)
                               cATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTc                                                                                                               <  2:1188569/151‑1 (MQ=255)
                                aTGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCa                                                                                                              <  1:1109241/151‑1 (MQ=255)
                                  gCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAac                                                                                                            >  3:449424/1‑151 (MQ=255)
                                    cGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAacac                                                                                                          >  2:897698/1‑151 (MQ=255)
                                         aTACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGc                                                                                                     >  4:122241/1‑151 (MQ=255)
                                          tACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGcc                                                                                                    >  3:36935/1‑151 (MQ=255)
                                           acgcgacgcgATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACCCCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGcc                                                                                                    <  3:204328/150‑1 (MQ=255)
                                            cgcgacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCgg                                                                                                  <  1:908745/151‑1 (MQ=255)
                                            cgcgacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCg                                                                                                   <  2:818125/150‑1 (MQ=255)
                                              cgacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGt                                                                                                 >  1:924139/1‑150 (MQ=255)
                                              ccacgcgaTGCGGTCCGGATGGAACGGCTACAGCGCCTCAGTTTTCTCGCCATCACCGAGGAGCTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGt                                                                                                 <  1:520637/148‑1 (MQ=255)
                                               gacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTaa                                                                                               <  2:378402/151‑1 (MQ=255)
                                               gacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTaa                                                                                               <  1:853585/151‑1 (MQ=255)
                                                 cgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACTAATAGAGAGCACCGCTCCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGc                                                                                             <  1:787760/151‑1 (MQ=255)
                                                  gcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGc                                                                                             <  2:1077851/150‑1 (MQ=255)
                                                  gcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCg                                                                                            >  1:261309/1‑151 (MQ=255)
                                                   cgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCgg                                                                                           >  2:486995/1‑151 (MQ=255)
                                                    gaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGt                                                                                          >  1:744866/1‑151 (MQ=255)
                                                     aTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCgg                                                                                           <  1:1248063/149‑1 (MQ=255)
                                                     aTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGt                                                                                          >  2:1129918/1‑150 (MQ=255)
                                                       gCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAAc                                                                                       <  2:178726/151‑1 (MQ=255)
                                                       gCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAAc                                                                                       <  2:1214939/151‑1 (MQ=255)
                                                          gTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCt                                                                                    <  2:1195074/151‑1 (MQ=255)
                                                          gTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCt                                                                                    >  3:80174/1‑151 (MQ=255)
                                                          gTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCt                                                                                    <  1:335855/151‑1 (MQ=255)
                                                          gTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCt                                                                                    <  1:310771/151‑1 (MQ=255)
                                                           tCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGCTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCtt                                                                                   >  3:78016/1‑151 (MQ=255)
                                                            ccGGATGGAACGGCTCCAGCGCCTCAGTTTCCTCGCCAACACCGAGGAACTTTATCGGTTGGCCAGTGATGTGACAAATAGAGAGCACCCCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTc                                                                                  <  2:1274249/151‑1 (MQ=255)
                                                              ggATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCAt                                                                                >  1:81534/1‑151 (MQ=255)
                                                                aTGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCAtt                                                                               <  2:1066345/150‑1 (MQ=255)
                                                                aTGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTg                                                                              <  2:513341/151‑1 (MQ=255)
                                                                       ggCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCtt                                                                       <  1:403597/151‑1 (MQ=255)
                                                                           cgaGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGGTTGCCAGGGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGGCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGc                                                                   >  2:527940/3‑151 (MQ=255)
                                                                             aGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCAGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCg                                                                 <  1:1121682/151‑1 (MQ=255)
                                                                              gcgcCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGccca                                                                <  2:640091/151‑3 (MQ=255)
                                                                                  cTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTg                                                            >  1:1116754/1‑151 (MQ=255)
                                                                                    cAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTgg                                                           <  2:790116/150‑1 (MQ=255)
                                                                                    cAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTg                                                            <  2:465153/149‑1 (MQ=255)
                                                                                      gTTTTCTCGCCAACGCCGAGGAACTTGATCGGTTTGCCAGGGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGcc                                                         <  4:208604/150‑1 (MQ=255)
                                                                                         ttCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCa                                                      <  2:467839/150‑1 (MQ=255)
                                                                                             cGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATc                                                    <  2:230468/148‑1 (MQ=255)
                                                                                             cGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCt                                                  <  3:132031/150‑1 (MQ=255)
                                                                                              gCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGa                                                <  4:422272/151‑1 (MQ=255)
                                                                                                cAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGAcc                                              >  1:394070/1‑151 (MQ=255)
                                                                                                     ccGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTct                                         <  2:439836/151‑2 (MQ=255)
                                                                                                     ccGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCa                                         <  2:848595/151‑1 (MQ=255)
                                                                                                     ccGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCa                                         <  2:463004/151‑1 (MQ=255)
                                                                                                       gAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCAt                                        <  1:1083996/150‑1 (MQ=255)
                                                                                                       gAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATg                                       <  2:1110050/151‑1 (MQ=255)
                                                                                                       gAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATg                                       >  1:420042/1‑151 (MQ=255)
                                                                                                          gAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCg                                    <  1:739435/151‑1 (MQ=255)
                                                                                                                 aTCGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAAcc                              >  4:388354/1‑150 (MQ=255)
                                                                                                                   cGGTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACcac                            >  2:523078/1‑150 (MQ=255)
                                                                                                                    ggTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACaa                          <  3:64047/151‑1 (MQ=255)
                                                                                                                     gTTTGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACaa                          >  2:122800/1‑150 (MQ=255)
                                                                                                                       ttGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAAc                        <  2:93359/150‑1 (MQ=255)
                                                                                                                       ttGCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAAc                        <  1:560692/150‑1 (MQ=255)
                                                                                                                         gCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAgg                     <  4:249797/151‑1 (MQ=255)
                                                                                                                         gCCAGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTAAACCACAAACAgg                     >  2:590856/1‑151 (MQ=255)
                                                                                                                            aGTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGtt                  <  3:113646/151‑1 (MQ=255)
                                                                                                                             gTGATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGttt                 <  3:322145/151‑1 (MQ=255)
                                                                                                                               gATGTGACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTc                <  2:413527/150‑1 (MQ=255)
                                                                                                                                  gtgACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAAcc            <  2:1167333/151‑1 (MQ=255)
                                                                                                                                   tgACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCg           >  3:366896/1‑151 (MQ=255)
                                                                                                                                    gACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCgg          >  1:880436/1‑151 (MQ=255)
                                                                                                                                    gACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCgg          >  1:854345/1‑151 (MQ=255)
                                                                                                                                    gACGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCgg          <  2:932976/151‑1 (MQ=255)
                                                                                                                                     aCGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCggg         >  4:78441/1‑151 (MQ=255)
                                                                                                                                     aCGAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCggg         <  3:302083/151‑1 (MQ=255)
                                                                                                                                       gAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGtt       <  4:330912/151‑1 (MQ=255)
                                                                                                                                       gAATAGAGAGCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGtt       >  2:441779/1‑151 (MQ=255)
                                                                                                                                           agagagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATc   >  2:324380/1‑151 (MQ=255)
                                                                                                                                             agagCACCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCg  >  4:224069/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCG  >  NC_000913/2746874‑2747164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: