Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 318,798 T→C M402V (ATG→GTG)  rclA ← reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913318,7980TC99.0% 378.3 / NA 99M402V (ATG→GTG) rclAreactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (49/49);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGC  >  NC_000913/318653‑318945
                                                                                                                                                 |                                                                                                                                                   
tGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTGTAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGt                                                                                                                                                >  3:304931/1‑151 (MQ=255)
tGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCg                                                                                                                                                 <  2:797495/150‑1 (MQ=255)
tGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGt                                                                                                                                                >  2:926296/1‑151 (MQ=255)
 gTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGt                                                                                                                                                <  2:424206/150‑1 (MQ=255)
 gTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGt                                                                                                                                                <  2:58606/150‑1 (MQ=255)
  ttCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTgg                                                                                                                                              >  4:110681/1‑151 (MQ=255)
  ttCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTgg                                                                                                                                              >  1:876825/1‑151 (MQ=255)
      ggCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAg                                                                                                                                           >  3:68192/1‑150 (MQ=255)
       gCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTc                                                                                                                                         <  1:981799/151‑1 (MQ=255)
       gCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTc                                                                                                                                         >  1:598555/1‑151 (MQ=255)
        cGTCTGATTTTGAGTTTATTTGACTACTGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTc                                                                                                                                         >  2:573232/1‑150 (MQ=255)
        cGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCa                                                                                                                                        <  2:654549/151‑1 (MQ=255)
         gTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCaa                                                                                                                                       <  2:308641/151‑1 (MQ=255)
         gTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCa                                                                                                                                        <  1:135337/150‑1 (MQ=255)
          tCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAac                                                                                                                                      >  2:888788/1‑151 (MQ=255)
             gATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAaca                                                                                                                                     <  2:1070235/149‑1 (MQ=255)
              aTTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAacacac                                                                                                                                  <  2:1048922/151‑1 (MQ=255)
                   gAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACAcagca                                                                                                                              <  2:102013/150‑1 (MQ=255)
                   gAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGACGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACAcagca                                                                                                                              <  1:772039/150‑1 (MQ=255)
                    aGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCTCCTCGTGGGAGTCAACACACAGCAGt                                                                                                                            <  2:1002802/151‑1 (MQ=255)
                    aGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGt                                                                                                                            >  1:543646/1‑151 (MQ=255)
                       ttATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGa                                                                                                                          <  1:771501/150‑1 (MQ=255)
                       ttATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGAt                                                                                                                         >  3:323123/1‑151 (MQ=255)
                         aTTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATg                                                                                                                        <  4:381464/150‑1 (MQ=255)
                         aTTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGc                                                                                                                       >  2:164655/1‑151 (MQ=255)
                           ttGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGccc                                                                                                                     >  3:282122/1‑151 (MQ=255)
                           ttGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGccc                                                                                                                     >  4:59786/1‑151 (MQ=255)
                             gACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGcccc                                                                                                                    <  1:223897/150‑1 (MQ=255)
                                  aTGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACAt                                                                                                              >  1:249227/1‑151 (MQ=255)
                                       aaTAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGtt                                                                                                         <  2:947506/151‑1 (MQ=255)
                                          aGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTgg                                                                                                       <  2:764056/150‑1 (MQ=255)
                                          aGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTgg                                                                                                       <  2:911860/150‑1 (MQ=255)
                                              cATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGttt                                                                                                   <  1:1125281/150‑1 (MQ=255)
                                              cATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGttt                                                                                                   <  2:71454/150‑1 (MQ=255)
                                                 tGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTttat                                                                                                <  4:5064/150‑1 (MQ=255)
                                                  gAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTttatt                                                                                               >  2:702141/1‑150 (MQ=255)
                                                    gTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATc                                                                                            <  1:888963/151‑1 (MQ=255)
                                                      gATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATca                                                                                           <  2:1138451/150‑1 (MQ=255)
                                                          cGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATcaacaa                                                                                       <  2:681842/150‑1 (MQ=255)
                                                           gCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATc                                                                                     <  1:318135/151‑1 (MQ=255)
                                                           gCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGAGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATc                                                                                     >  4:152069/1‑151 (MQ=255)
                                                              cATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCg                                                                                    <  1:418033/149‑1 (MQ=255)
                                                              cATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGc                                                                                   <  2:1077181/150‑1 (MQ=255)
                                                                  gACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCtttt                                                                               >  2:113473/1‑150 (MQ=255)
                                                                  gACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATAACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCttt                                                                                >  2:357353/1‑149 (MQ=255)
                                                                   aCGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTa                                                                              >  1:226210/1‑150 (MQ=255)
                                                                   aCGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTa                                                                              >  2:169560/1‑150 (MQ=255)
                                                                    cGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAAt                                                                            <  2:1220777/151‑1 (MQ=255)
                                                                    cGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAAt                                                                            <  1:1282272/151‑1 (MQ=255)
                                                                    cGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGAGGGAGTCAACACACAGCAGTGATGCCCCT‑ACATTAGTTTGGTTTTATTATCAACAAACGCCTTTAAt                                                                            >  3:457955/1‑150 (MQ=255)
                                                                        tGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATAccc                                                                        >  3:439734/1‑151 (MQ=255)
                                                                          aGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCa                                                                      <  3:154348/151‑1 (MQ=255)
                                                                          aGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCa                                                                      <  2:294208/151‑1 (MQ=255)
                                                                           gTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTAGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCAc                                                                     >  2:1139342/1‑151 (MQ=255)
                                                                            tAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACg                                                                    >  1:1074094/1‑151 (MQ=255)
                                                                             aaaTATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACg                                                                    <  1:400617/150‑1 (MQ=255)
                                                                             aaaTATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACg                                                                    >  3:153952/1‑150 (MQ=255)
                                                                             aaaTATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGa                                                                   <  1:164679/151‑1 (MQ=255)
                                                                              aaTATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAg                                                                  <  2:38832/151‑1 (MQ=255)
                                                                                  tCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtc                                                              >  1:884689/1‑151 (MQ=255)
                                                                                     gATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAt                                                               <  2:26989/147‑1 (MQ=255)
                                                                                      aTCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtcattc                                                          >  2:927778/1‑151 (MQ=255)
                                                                                        cgcgTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTAtcattcat                                                        >  4:343192/1‑151 (MQ=255)
                                                                                          cgTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATtcatc                                                       >  4:334545/1‑150 (MQ=255)
                                                                                           gTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCAc                                                     >  4:379407/1‑151 (MQ=255)
                                                                                           gTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCAc                                                     >  1:46327/1‑151 (MQ=255)
                                                                                                atGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTg                                                 <  1:322244/150‑1 (MQ=255)
                                                                                                   cTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAc                                             >  1:567275/1‑151 (MQ=255)
                                                                                                   cTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAc                                             >  2:770863/1‑151 (MQ=255)
                                                                                                    tataAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcg                                            >  2:1108889/1‑151 (MQ=255)
                                                                                                     ataAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcgc                                           <  1:1175403/151‑1 (MQ=255)
                                                                                                     ataAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcgc                                           >  1:186423/1‑151 (MQ=255)
                                                                                                       aaGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcgcg                                          <  1:1121694/150‑1 (MQ=255)
                                                                                                       aaGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCgg                                         <  1:734835/151‑1 (MQ=255)
                                                                                                            aGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTg                                    >  2:897608/1‑151 (MQ=255)
                                                                                                            aGCCCGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTg                                    <  2:1210336/151‑1 (MQ=255)
                                                                                                              cccGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGc                                   <  1:589420/150‑1 (MQ=255)
                                                                                                              cccGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCa                                  <  2:1007461/151‑1 (MQ=255)
                                                                                                               ccGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGc                                   >  1:297914/1‑149 (MQ=255)
                                                                                                                cGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGc                                >  4:276323/1‑151 (MQ=255)
                                                                                                                cGGCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGc                                >  4:234884/1‑151 (MQ=255)
                                                                                                                  gCATCCATCACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCt                               >  1:46426/1‑150 (MQ=255)
                                                                                                                   catccatcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                             >  2:705647/1‑151 (MQ=255)
                                                                                                                    atccatcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                             >  3:412926/1‑150 (MQ=255)
                                                                                                                    atccatcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACa                            <  4:262266/151‑1 (MQ=255)
                                                                                                                     tccatcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                             >  1:521895/1‑149 (MQ=255)
                                                                                                                      ccatcACCATTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAgg                          <  2:667832/151‑1 (MQ=255)
                                                                                                                              aTTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTc                   >  1:823337/1‑150 (MQ=255)
                                                                                                                              aTTTTCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTc                   >  2:43012/1‑150 (MQ=255)
                                                                                                                                 ttCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTcacc                <  2:967522/150‑1 (MQ=255)
                                                                                                                                  tCACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTcacca               <  4:323127/150‑1 (MQ=255)
                                                                                                                                   cACTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCaccacc             <  2:704018/151‑1 (MQ=255)
                                                                                                                                    aCTATATTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCt            >  1:1090772/1‑151 (MQ=255)
                                                                                                                                      tataTTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTg           >  2:435779/1‑150 (MQ=255)
                                                                                                                                      tataTTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTg           >  1:516331/1‑150 (MQ=255)
                                                                                                                                      tataTTGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTg           >  4:196131/1‑150 (MQ=255)
                                                                                                                                           tGATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATc     >  2:700811/1‑151 (MQ=255)
                                                                                                                                            gATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCa    <  1:842015/151‑1 (MQ=255)
                                                                                                                                            gATCACCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCa    <  2:688057/151‑1 (MQ=255)
                                                                                                                                              tcaccTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGc  >  4:59124/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGTTCTGGCGTCTGATTTTGAGTTTATTTGACTAATGAAAATAGATCATTGAGTGATTCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGC  >  NC_000913/318653‑318945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑