BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,122,268	T→C	D513G (GAC→GGC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,122,268	0	T	C	100.0%	431.5 / NA	115	D513G (GAC→GGC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/62); total (53/62)

Only 100 of 116 total aligned reads displayed.

CTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATC  >  NC_000913/3122120‑3122414

cTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGccc                                                                                                                                                  >  2:886149/1‑151 (MQ=255)

   ggCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGc                                                                                                                                               <  3:247138/151‑1 (MQ=255)

     cAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCa                                                                                                                                              >  2:30393/1‑150 (MQ=255)

     cAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCAt                                                                                                                                             <  4:71296/151‑1 (MQ=255)

      aaTGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCAtt                                                                                                                                            <  2:1194220/151‑1 (MQ=255)

       aTGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTg                                                                                                                                           <  3:337543/151‑1 (MQ=255)

         gTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGcc                                                                                                                                         <  2:894778/151‑1 (MQ=255)

           tGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGccc                                                                                                                                        >  1:774430/1‑150 (MQ=255)

           tGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGccc                                                                                                                                        <  1:792885/150‑1 (MQ=255)

           tGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGccc                                                                                                                                        <  2:735373/150‑1 (MQ=255)

            ggCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCcac                                                                                                                                      <  1:153744/151‑1 (MQ=255)

            ggCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCcac                                                                                                                                      >  3:52155/1‑151 (MQ=255)

            ggCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCca                                                                                                                                       <  1:1201903/150‑1 (MQ=255)

             gCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCcac                                                                                                                                      >  2:734169/1‑150 (MQ=255)

              cTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACAt                                                                                                                                    >  1:377012/1‑151 (MQ=255)

                tGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACAt                                                                                                                                    <  2:647427/149‑1 (MQ=255)

                 gTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGcc                                                                                                                                 >  1:1268924/1‑151 (MQ=255)

                  tACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCt                                                                                                                                >  1:150540/1‑151 (MQ=255)

                   aCGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCtt                                                                                                                               <  1:1218825/151‑1 (MQ=255)

                   aCGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCtt                                                                                                                               <  1:886324/151‑1 (MQ=255)

                   aCGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCt                                                                                                                                <  3:269259/150‑1 (MQ=255)

                     gCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCttt                                                                                                                              >  2:936434/1‑150 (MQ=255)

                       tctGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTAcc                                                                                                                           >  4:181861/1‑151 (MQ=255)

                         tGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCa                                                                                                                          >  1:1201493/1‑150 (MQ=255)

                            aCGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAAttt                                                                                                                      >  1:522841/1‑151 (MQ=255)

                              gTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgc                                                                                                                    >  1:280440/1‑151 (MQ=255)

                              gTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGGTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgc                                                                                                                    >  2:1215146/1‑151 (MQ=255)

                                tggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTgcgc                                                                                                                  >  2:267000/1‑151 (MQ=255)

                                 ggtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCt                                                                                                                 >  1:142036/1‑151 (MQ=255)

                                  gtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCtt                                                                                                                <  4:282552/151‑1 (MQ=255)

                                  gtttggtGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCtt                                                                                                                >  4:165381/1‑151 (MQ=255)

                                     tggtggTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTATCAATTTGCGCTTTa                                                                                                              <  3:45482/150‑1 (MQ=255)

                                     tggtggTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTAc                                                                                                             >  3:42812/1‑151 (MQ=255)

                                       gtggtAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACcg                                                                                                           >  1:753786/1‑151 (MQ=255)

                                         ggtAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACcgcg                                                                                                         <  1:62505/151‑1 (MQ=255)

                                         ggtAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACcgcg                                                                                                         <  2:1080171/151‑1 (MQ=255)

                                          gtAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCgcgc                                                                                                        <  2:158178/151‑1 (MQ=255)

                                            aGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCa                                                                                                       <  1:787279/150‑1 (MQ=255)

                                                cAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGccc                                                                                                   >  2:1000441/1‑150 (MQ=255)

                                                 aGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCcac                                                                                                 <  1:541522/151‑1 (MQ=255)

                                                  gcgcATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCcac                                                                                                 >  2:693384/1‑150 (MQ=255)

                                                        ggAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCacagaacag                                                                                          <  2:670036/151‑1 (MQ=255)

                                                        ggAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCacagaaca                                                                                           <  1:120787/150‑1 (MQ=255)

                                                          tgCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCATTTGGTCGCCCTTCTGGCTGTACATGTCTGGCATCAGGCCCGGCATTGCCCACATGCCTTTACTAATTTGCGCTTTACCGCGCAGCCCacagaacag                                                                                          >  2:573124/2‑149 (MQ=255)

                                                             gTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCgg                                                                                     >  4:164368/1‑151 (MQ=255)

                                                              tAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCgg                                                                                     >  3:116490/1‑150 (MQ=255)

                                                              tAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCgg                                                                                     >  1:333587/1‑150 (MQ=255)

                                                              tAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCgg                                                                                     >  2:455226/1‑150 (MQ=255)

                                                               agcagcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGaa                                                                                   <  1:425649/151‑1 (MQ=255)

                                                                  agcGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGc                                                                                >  1:945336/1‑151 (MQ=255)

                                                                    cGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCAc                                                                              >  4:159375/1‑151 (MQ=255)

                                                                        tGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGtt                                                                           <  2:1230149/150‑1 (MQ=255)

                                                                         ggTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGtta                                                                          <  2:906866/150‑1 (MQ=255)

                                                                            gACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGttatta                                                                       <  3:312786/150‑1 (MQ=255)

                                                                            gACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTAc                                                                      <  3:315865/151‑1 (MQ=255)

                                                                            gACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTAc                                                                      >  1:1248055/1‑151 (MQ=255)

                                                                               tgAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCt                                                                   >  3:396233/2‑151 (MQ=255)

                                                                               ggAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCt                                                                   >  2:1137536/1‑151 (MQ=255)

                                                                               ggAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCt                                                                   <  1:633984/151‑1 (MQ=255)

                                                                                    ccAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGt                                                                >  3:144567/1‑149 (MQ=255)

                                                                                     cAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAgg                                                             <  3:353186/151‑1 (MQ=255)

                                                                                        gCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCtt                                                          <  3:111587/151‑1 (MQ=255)

                                                                                        gCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCtt                                                          >  1:635698/1‑151 (MQ=255)

                                                                                           gtgtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTg                                                        <  3:410591/150‑1 (MQ=255)

                                                                                             gtTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATc                                                     <  1:649559/151‑1 (MQ=255)

                                                                                                 gCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAg                                                 <  1:503605/151‑1 (MQ=255)

                                                                                                 gCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAg                                                 <  3:350027/151‑1 (MQ=255)

                                                                                                 gCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATGTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAg                                                 <  2:1142515/151‑1 (MQ=255)

                                                                                                   cccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGc                                               <  1:498844/151‑1 (MQ=255)

                                                                                                    ccGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCg                                              >  1:634027/1‑151 (MQ=255)

                                                                                                      ggCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTc                                            <  2:271025/151‑1 (MQ=255)

                                                                                                       gCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCg                                           >  1:317003/1‑151 (MQ=255)

                                                                                                         aCGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGa                                          <  4:232933/150‑1 (MQ=255)

                                                                                                         aCGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGAt                                         >  1:1093801/1‑151 (MQ=255)

                                                                                                           gCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGAttt                                       >  2:1071822/1‑151 (MQ=255)

                                                                                                              gTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCAt                                    <  4:42715/151‑1 (MQ=255)

                                                                                                                 ggTCTCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTg                                 >  1:1212607/1‑151 (MQ=255)

                                                                                                                 ggTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTg                                 <  1:166488/151‑1 (MQ=255)

                                                                                                                      cccTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGAtttt                            <  2:453085/151‑1 (MQ=255)

                                                                                                                       ccTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGAttttt                           >  3:113622/1‑151 (MQ=255)

                                                                                                                        cTTCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGAttttt                           >  1:601422/1‑150 (MQ=255)

                                                                                                                         ttCTGGCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTAc                         >  1:352584/1‑151 (MQ=255)

                                                                                                                              gCTGTACATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgcagc                    <  4:44455/151‑1 (MQ=255)

                                                                                                                               gtgcaCATGTCCGCCATCAGGCGCGGCAGTGCACACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACgcagc                    >  2:859785/5‑150 (MQ=255)

                                                                                                                                   aCATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCgg               >  2:199133/1‑151 (MQ=255)

                                                                                                                                   aCATGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCg                <  2:1265488/150‑1 (MQ=255)

                                                                                                                                     aTGTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGc             >  1:1207230/1‑151 (MQ=255)

                                                                                                                                       gTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGcc            >  2:960557/1‑150 (MQ=255)

                                                                                                                                       gTCTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCa           <  3:96911/151‑1 (MQ=255)

                                                                                                                                         cTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGc         >  2:130135/1‑151 (MQ=255)

                                                                                                                                         cTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGc         >  1:304828/1‑151 (MQ=255)

                                                                                                                                         cTGCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGc         >  1:565950/1‑151 (MQ=255)

                                                                                                                                           gCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTTCCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGc         <  2:682401/149‑1 (MQ=255)

                                                                                                                                           gCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAg          <  2:258239/148‑1 (MQ=255)

                                                                                                                                           gCCATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCtt       >  2:507187/1‑151 (MQ=255)

                                                                                                                                            ccATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTc      >  3:296136/1‑151 (MQ=255)

                                                                                                                                             cATCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTcc     <  4:194523/151‑1 (MQ=255)

                                                                                                                                               tCAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCa    <  1:638596/150‑1 (MQ=255)

                                                                                                                                                cAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATc  <  2:376947/151‑1 (MQ=255)

                                                                                                                                                cAGGCCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATc  >  4:35380/1‑151 (MQ=255)

CTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCGGCACGCAGTTGGTCGCCCTTCTGGCTGTACATGTCTGCCATCAGGTCCGGCATTGCCCACATGCCTTTACCAATTTGCGCTTTACCGCGCAGCCCACAGAACAGACCGGAAAGCACGTTATTACGCTCGTAGGCTTTGATCCAAGGCGTCGATTTCATCTGATTTTTACGCAGCATCGGGCCAGCTTCCATC  >  NC_000913/3122120‑3122414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`