BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,019	G→A	G302D (GGC→GAC)	tolC →	transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,019	0	G	A	99.1%	439.7 / NA	114	G302D (GGC→GAC)	tolC	transport channel
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (47/66); total (48/66)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTT  >  NC_000913/3178871‑3179165

gACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGAcc                                                                                                                                                  <  1:863270/151‑1 (MQ=255)

gACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGCGCCGCTGGTACCCAGTATGACGATAGCAATATGGAcc                                                                                                                                                  >  1:508540/1‑151 (MQ=255)

 aCCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGAcc                                                                                                                                                  >  1:345292/1‑150 (MQ=255)

    tGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCCGGTACCCAGTATGACGATAGCAATATGGACCAGa                                                                                                                                               >  2:1081413/1‑150 (MQ=255)

     ggCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGaa                                                                                                                                              <  2:288230/150‑1 (MQ=255)

     ggCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGa                                                                                                                                               <  3:58761/149‑1 (MQ=255)

        gcgcgAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACaa                                                                                                                                           >  2:503562/1‑150 (MQ=255)

           cgAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGtt                                                                                                                                       <  3:246597/151‑1 (MQ=255)

            gAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTg                                                                                                                                      <  4:173243/151‑1 (MQ=255)

             aGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTgg                                                                                                                                     <  2:976332/151‑1 (MQ=255)

             aGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTgg                                                                                                                                     >  1:43827/1‑151 (MQ=255)

             aGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTgg                                                                                                                                     <  1:419800/151‑1 (MQ=255)

              gCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTgg                                                                                                                                     <  2:982531/150‑1 (MQ=255)

              gCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGc                                                                                                                                    >  4:376129/1‑151 (MQ=255)

              gCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGc                                                                                                                                    <  2:625408/151‑1 (MQ=255)

                  aTTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTg                                                                                                                                 >  3:174691/1‑150 (MQ=255)

                  aTTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTg                                                                                                                                 >  2:1096385/1‑150 (MQ=255)

                     cGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCt                                                                                                                             <  3:236908/151‑1 (MQ=255)

                       ccAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTtc                                                                                                                           <  1:179923/151‑1 (MQ=255)

                         aGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTtctc                                                                                                                         <  3:179087/151‑1 (MQ=255)

                          ggCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCTGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGTCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGTGCTtctc                                                                                                                         <  3:257566/150‑1 (MQ=255)

                           gcgcAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGc                                                                                                                       <  4:138010/151‑1 (MQ=255)

                             gcAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTg                                                                                                                     <  2:976988/151‑1 (MQ=255)

                             gcAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTg                                                                                                                     >  2:623097/1‑151 (MQ=255)

                               aGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGcc                                                                                                                   >  4:222031/1‑151 (MQ=255)

                                ggATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCg                                                                                                                  <  2:611493/151‑1 (MQ=255)

                                ggATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCg                                                                                                                  <  2:1215547/151‑1 (MQ=255)

                                 gATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCg                                                                                                                  >  1:1089534/1‑150 (MQ=255)

                                   tGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGAtt                                                                                                               >  2:350192/1‑151 (MQ=255)

                                    ggTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGAttt                                                                                                              >  3:173122/1‑151 (MQ=255)

                                           tACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAg                                                                                                         <  4:59862/149‑1 (MQ=255)

                                                aCTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGa                                                                                                   >  4:87385/1‑150 (MQ=255)

                                                       aTTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGtt                                                                                             <  2:1056659/149‑1 (MQ=255)

                                                       aTTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTaa                                                                                           <  1:350682/151‑1 (MQ=255)

                                                       aTTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTaa                                                                                           <  3:208011/151‑1 (MQ=255)

                                                        tttAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAAc                                                                                          >  4:226405/1‑151 (MQ=255)

                                                             cGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCa                                                                                     <  2:119584/151‑1 (MQ=255)

                                                                      ccGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAAc                                                                             >  2:61471/1‑150 (MQ=255)

                                                                      ccGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACa                                                                            <  3:388320/151‑1 (MQ=255)

                                                                        gggATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAgg                                                                          <  1:577448/151‑1 (MQ=255)

                                                                         ggATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGc                                                                         <  1:818276/151‑1 (MQ=255)

                                                                          gATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGc                                                                         >  1:154217/1‑150 (MQ=255)

                                                                           aTTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGcac                                                                       <  3:189078/151‑1 (MQ=255)

                                                                             ttttGACAACTCTTATAGCGGTTCGAAAAACCCTGGTGCCGCTGGTACCCACTATGACGATAGCAATATGGACCAGAAAAAAATTGGCCTGAGCTTCTCGCTGCCGATTTTTCAGGGCGGGATGGTTAACTCGCAGGGGAAACAGGcac                                                                       >  3:99670/4‑149 (MQ=255)

                                                                             ttCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGcac                                                                       >  2:1030935/1‑149 (MQ=255)

                                                                             ttCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGcac                                                                       >  2:831984/1‑149 (MQ=255)

                                                                             ttCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGca                                                                        >  2:791142/1‑148 (MQ=255)

                                                                                tGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTAc                                                                  >  1:1251582/1‑151 (MQ=255)

                                                                                  acacCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACa                                                                 >  2:253598/1‑150 (MQ=255)

                                                                                    acCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACt                                                              >  4:22413/1‑151 (MQ=255)

                                                                                     ccTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACtt                                                             >  2:1187308/1‑151 (MQ=255)

                                                                                      ctctTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACttt                                                            >  3:150472/1‑151 (MQ=255)

                                                                                       tctTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTg                                                           >  4:145165/1‑151 (MQ=255)

                                                                                        ctTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACttt                                                            <  3:132417/149‑1 (MQ=255)

                                                                                          tataGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGGCGATGTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTg                                                           <  1:1197690/148‑1 (MQ=255)

                                                                                          tataGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTg                                                           <  3:451741/148‑1 (MQ=255)

                                                                                           ataGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCgg                                                       <  1:961849/151‑1 (MQ=255)

                                                                                             aGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTg                                                     <  1:537987/151‑1 (MQ=255)

                                                                                                ggTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGcc                                                   <  1:1187524/150‑1 (MQ=255)

                                                                                                ggTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCa                                                  <  2:1160914/151‑1 (MQ=255)

                                                                                                ggTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCa                                                  <  1:674704/151‑1 (MQ=255)

                                                                                                   tCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGc                                                <  4:436857/150‑1 (MQ=255)

                                                                                                   tCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGc                                                <  2:950941/150‑1 (MQ=255)

                                                                                                   tCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCg                                               >  1:892054/1‑151 (MQ=255)

                                                                                                    cGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCg                                               <  4:321354/150‑1 (MQ=255)

                                                                                                     gAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCg                                               >  1:218292/1‑149 (MQ=255)

                                                                                                      aaaaCCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGc                                            >  2:1218404/1‑151 (MQ=255)

                                                                                                          cccGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACt                                        <  4:332418/151‑1 (MQ=255)

                                                                                                            tgtggtgCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTgg                                      >  2:844259/2‑151 (MQ=255)

                                                                                                               ggtgCCGCTGGTACCCAGTATGACGATGGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGaaa                                   <  4:194980/151‑1 (MQ=255)

                                                                                                               ggtgCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGaaa                                   <  1:540955/151‑1 (MQ=255)

                                                                                                               ggtgCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGaa                                    >  1:211774/1‑150 (MQ=255)

                                                                                                                  gCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGt                                 <  4:405044/150‑1 (MQ=255)

                                                                                                                  gCCGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGt                                 <  2:352956/150‑1 (MQ=255)

                                                                                                                   ccGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGc                               >  2:557230/1‑151 (MQ=255)

                                                                                                                    cGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGcc                              <  1:326631/151‑1 (MQ=255)

                                                                                                                    cGCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGcc                              >  3:104991/1‑151 (MQ=255)

                                                                                                                     gCTGGTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGccc                             >  1:994767/1‑151 (MQ=255)

                                                                                                                        ggTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCAt                           <  4:337750/150‑1 (MQ=255)

                                                                                                                        ggTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATc                          <  1:700915/151‑1 (MQ=255)

                                                                                                                        ggTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATc                          <  1:1044427/151‑1 (MQ=255)

                                                                                                                         gTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCg                         <  3:238756/151‑1 (MQ=255)

                                                                                                                         gTACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCg                         <  1:1101389/151‑1 (MQ=255)

                                                                                                                          tACCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCCCAGTACAACTTTGTCGGTGCCAGCGAGCACCTGGAAAGTGCCCATCg                         <  3:227074/150‑1 (MQ=255)

                                                                                                                           aCCCAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGt                        <  4:315445/150‑1 (MQ=255)

                                                                                                                             ccAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGACCATCGTAGc                     <  1:981028/151‑1 (MQ=255)

                                                                                                                              cAGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcg                    >  1:646372/1‑151 (MQ=255)

                                                                                                                               aGTATGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgt                   <  1:832152/151‑1 (MQ=255)

                                                                                                                                  aTGACGATAGCAATATGGGCCAGAACACAGCTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTACCTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgtcg                 >  2:950463/1‑150 (MQ=255)

                                                                                                                                   tGACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTg               >  3:95019/1‑151 (MQ=255)

                                                                                                                                    gACGATAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGc              <  1:941542/151‑1 (MQ=255)

                                                                                                                                         tAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCg                         <  2:560209/135‑1 (MQ=255)

                                                                                                                                         tAGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCg                         >  1:560316/1‑135 (MQ=255)

                                                                                                                                          aGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGAcc         <  3:398134/150‑1 (MQ=255)

                                                                                                                                          aGCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCg        <  1:762453/151‑1 (MQ=255)

                                                                                                                                           gCAATATGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGt       <  1:1096159/151‑1 (MQ=255)

                                                                                                                                                atGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGtt  >  4:185059/1‑151 (MQ=255)

                                                                                                                                                atGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGtt  >  2:628313/1‑151 (MQ=255)

                                                                                                                                                atGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGt   <  2:967202/150‑1 (MQ=255)

                                                                                                                                                atGGACCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGt   <  1:316043/150‑1 (MQ=255)

GACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTT  >  NC_000913/3178871‑3179165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`