Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,370,354 A→G V228A (GTG→GCG)  nanE ← putative N‑acetylmannosamine‑6‑P epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,3540AG100.0% 364.9 / NA 99V228A (GTG→GCG) nanEputative N‑acetylmannosamine‑6‑P epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/61);  total (38/61)

CTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCG  >  NC_000913/3370206‑3370499
                                                                                                                                                      |                                                                                                                                                 
cTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGcc                                                                                                                                                 >  4:381861/1‑151 (MQ=255)
  aaTGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGcgccgc                                                                                                                                               <  4:16353/151‑1 (MQ=255)
     gCGGAAAAGGCATCACGAAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCGGCCCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCt                                                                                                                                             <  1:470147/150‑1 (MQ=255)
       ggATAAGGCATCACGCAGGTCTTCTGGTGTCTGGCTGGCGGGCGTAGGAAGTTCACGCCGTTCGCGGATCTGCCCTTCAGCGCCAATCAGCGCGGCGGCAATTTTATTTCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCtttt                                                                                                                                          <  3:406523/151‑1 (MQ=255)
        gATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCttttt                                                                                                                                         >  3:412128/1‑151 (MQ=255)
         aaaaGGCAGCACGAAGGGCTTCTGGTGTCGGGCGGGCTGGCGTAGGAAGTCCACGACGATCGCGGATCTGCCCGTCAGCGCCAACCGGCGCGGCGGAAGGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCttttt                                                                                                                                         <  3:138381/148‑1 (MQ=255)
         aTAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTc                                                                                                                                        >  1:1225096/1‑151 (MQ=255)
           aaGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGCCGTAGGAAGTTCACGACGATCGGGGTTCTGCCCGTCAGCCCCAATCAGCGCGGCGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCAt                                                                                                                                      <  1:1102987/151‑1 (MQ=255)
                  cACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCtg                                                                                                                                 <  4:122109/149‑1 (MQ=255)
                        aGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTAc                                                                                                                         >  4:394230/1‑151 (MQ=255)
                          gCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTAcc                                                                                                                        >  1:323641/1‑150 (MQ=255)
                                ggTTTCTGGCGGGCTGCCGAAGAGAGTACACGACGGTCGCGGATCTCCCCGTCGGCGCAAATCGGCGCGGGGGCAATTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCAc                                                                                                                      <  2:62510/146‑1 (MQ=255)
                                ggTGTTTGGCTGGCTGGCGTAGGAAGTTCACGACGCTCGCGGATCGGCCCGTAAGCGCCAATCGGCGGGGCGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACt                                                                                                                     <  1:856865/147‑1 (MQ=255)
                                ggTGTCGGGCTGGCGGGCGGAGGAAGTTCACGGGGATGGGGGAGCTGCCCGCAGGCGCCAAGCAGGGAGGGGGCAAGTTTAGTGCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGAc                                                                                                                  <  2:770108/150‑1 (MQ=255)
                                  tgtCTGGCTGGCTGGCGTAGGAATTTCACGACTATCGCGGATCTGCCCGCCAGCGCCAAACAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCAc                                                                                                                      <  1:438924/144‑1 (MQ=255)
                                    tCTGGCTGGCTGGCGTAGGAAGTTCACGCCGACCGCGGTTCTCCCCGCCAGCGCCAATCAGCGGGGCGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACaa                                                                                                                <  1:1122916/148‑1 (MQ=255)
                                     ctggctggctggCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAAtgt                                                                                                            >  2:1273167/1‑151 (MQ=255)
                                      tggctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAAtgtg                                                                                                           >  2:985861/1‑151 (MQ=255)
                                       ggctggctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCAAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACaaa                                                                                                               <  2:1089010/146‑1 (MQ=255)
                                       ggctggcgggcGTAGGAAGTCCACGACGATCGCGGATCTGCCCGTCAGCGCCAAACAGCGGGGCGGCAATTTTATTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACaaa                                                                                                               <  2:786284/146‑1 (MQ=255)
                                         ctggcgggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGGGGCGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCt                                                                                                         <  1:530239/150‑1 (MQ=255)
                                          tggctggcGTAGGATTTACACGTCGATGGCGGTTCTCCCCGCCGCCGCCAATCAGCGCGGGGGCAGGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCt                                                                                                         <  2:471573/149‑1 (MQ=255)
                                           ggctggcGGAGGATGTTCACGCGATTGGGGGATCTGCCCTTCGCCGCCAATCGGCGCGGGGGAAAGTTGAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCa                                                                                                       <  4:412134/150‑1 (MQ=255)
                                             ctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCa                                                                                                       >  1:1259306/1‑148 (MQ=255)
                                             ctggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCa                                                                                                       >  1:11799/1‑148 (MQ=255)
                                               ggcTTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCAAATCAGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGa                                                                                                    <  1:121543/149‑1 (MQ=255)
                                               ggcGTGGGAAGTCCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcg                                                                                                  <  1:124175/151‑1 (MQ=255)
                                               ggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGa                                                                                                    <  1:436141/149‑1 (MQ=255)
                                               ggcGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcg                                                                                                  <  4:431999/151‑1 (MQ=255)
                                                 cGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcgcg                                                                                                >  2:552426/1‑151 (MQ=255)
                                                   tAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTg                                                                                              >  3:434966/1‑151 (MQ=255)
                                                    aGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcgcg                                                                                                <  2:323589/148‑1 (MQ=255)
                                                     ggAAGTTCACGACGATCGCGGACCGGCCCGCCAGCGCCAATCAGCGGGGCGGAAATTTTTGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGAcgcg                                                                                                <  2:216404/147‑1 (MQ=255)
                                                       aaGTTCACGCCGATCGCGGATCTCCCCGTCAGCCCAAATAAGCGGGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGAtt                                                                                           <  2:855271/150‑1 (MQ=255)
                                                       aaGTTCACGACGTTCGCGGATCGGCCCGCCAGGGCCAATAGGCGGGGGGCAATTTTTAGTGCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGAta                                                                                           <  2:1063459/150‑2 (MQ=255)
                                                       aaGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTg                                                                                          <  2:656896/151‑1 (MQ=255)
                                                       aaGTGCCCGACGAGGGCGTTTCTCCCCGCCAGCCCAAATCAGCGCGGCGGCAAGTTTTGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTg                                                                                          <  2:1116996/151‑1 (MQ=255)
                                                       aaGTACACGCTGACCGCGGATCTGCCCGTCAGCGAAAATCAGCGGGGGGGAAAGTTTCGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGAtt                                                                                           <  1:621771/150‑1 (MQ=255)
                                                            cACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGa                                                                                      >  3:394457/1‑150 (MQ=255)
                                                               gacgaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAac                                                                                    <  1:690725/149‑1 (MQ=255)
                                                                ccgaTCGCGTCTCTCCCCTCCGGCCCCAATCAGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAacc                                                                                   <  2:88610/148‑1 (MQ=255)
                                                                acgtTCGCGGATGTCCCCGGCAGGCCCAATCCGGGCGGCGGTAGTTTTGTTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGa                                                                                      <  1:432703/146‑1 (MQ=255)
                                                                  gaTCGGGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                               <  4:225505/151‑1 (MQ=255)
                                                                  gaTCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGAAATTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgac                                                                                <  1:557021/150‑1 (MQ=255)
                                                                     tCGCGGATCTGCCCGTCAGCGCCAATCGGCGCGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAaccgacc                                                                               <  4:12283/149‑1 (MQ=255)
                                                                     tCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGt                                                                             >  2:200605/1‑151 (MQ=255)
                                                                      cgcgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                            >  1:1139208/1‑151 (MQ=255)
                                                                      cgcgGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTc                                                                            >  1:582582/1‑151 (MQ=255)
                                                                         ggATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCAcc                                                                         >  1:156902/1‑151 (MQ=255)
                                                                         ggATCTGCCCGTCAGCGCAAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCAcc                                                                         <  1:1277654/151‑1 (MQ=255)
                                                                                cccGTCAGCGCCAATCAGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAcg                                                                  <  1:1205213/151‑1 (MQ=255)
                                                                                cccGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGGTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCa                                                                    >  1:40060/1‑149 (MQ=255)
                                                                                cccGTCAGCGCCAATCAGCGCGGCGGAAAGTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGc                                                                       <  4:157993/146‑1 (MQ=255)
                                                                                cccGTCAGCGCCAATCAGCGCGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGc                                                                       <  2:445290/146‑1 (MQ=255)
                                                                                cccGTCAGCGCAAATCAGCGGGGCGGAAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGcc                                                                      <  1:772836/147‑1 (MQ=255)
                                                                                 ccGTCAGCCCCAACCGGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAcgc                                                                 <  2:1079092/151‑1 (MQ=255)
                                                                                   gTCACCGCCAACCGGCGGGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAcgcg                                                                <  2:1059770/150‑1 (MQ=255)
                                                                                     cAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCg                                                             >  2:441598/1‑151 (MQ=255)
                                                                                     cAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCg                                                             >  1:1016502/1‑151 (MQ=255)
                                                                                       gcgcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGt                                                            <  1:587364/150‑1 (MQ=255)
                                                                                       gcgcAATTCAGGGGGGGGCCAGGTTTAGTCCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCAcgcg                                                                <  1:1101752/146‑1 (MQ=255)
                                                                                        cgcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTgg                                                          <  4:443120/151‑1 (MQ=255)
                                                                                         gcCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGgc                                                         >  1:92957/1‑151 (MQ=255)
                                                                                         gcCAATCAGCGCGGCGGCAAGTTTAGGACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTTgc                                                         >  2:626125/1‑151 (MQ=255)
                                                                                          ccAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGgcg                                                        <  1:608259/151‑1 (MQ=255)
                                                                                                 gcgcGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGcgcatcgc                                                  >  1:1265523/1‑150 (MQ=255)
                                                                                                      gcggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgc                                            >  2:362300/1‑151 (MQ=255)
                                                                                                       cggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgcc                                           >  3:20779/1‑151 (MQ=255)
                                                                                                        ggcAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATccgcca                                          <  2:965924/151‑2 (MQ=255)
                                                                                                        ggaaaGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCgcatcgcatc                                               <  2:850297/143‑1 (MQ=255)
                                                                                                           aaGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCgccgcc                                        >  2:191680/1‑150 (MQ=255)
                                                                                                            aattttCGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCTCCGCCCACGCGCCGTGGCGCATCGCATCcgccgc                                         <  1:404769/146‑1 (MQ=255)
                                                                                                                ttAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAg                                    <  1:1232301/150‑1 (MQ=255)
                                                                                                                 tAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGc                                   >  3:15662/1‑150 (MQ=255)
                                                                                                                  aGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCa                                  <  1:528010/150‑1 (MQ=255)
                                                                                                                   gtgccgccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCa                                  <  3:329883/146‑1 (MQ=255)
                                                                                                                     aCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAg                                 <  2:869483/148‑1 (MQ=255)
                                                                                                                     aCCGCCGATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGc                               <  4:285275/150‑1 (MQ=255)
                                                                                                                       cgccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgtg                            <  1:552530/151‑1 (MQ=255)
                                                                                                                        gccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgtgt                           >  2:912559/1‑151 (MQ=255)
                                                                                                                        gccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgtgt                           >  4:409239/1‑151 (MQ=255)
                                                                                                                        gccgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCgtg                            >  3:437151/1‑150 (MQ=255)
                                                                                                                          cgATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgttg                         >  1:1175828/1‑151 (MQ=255)
                                                                                                                           gATATCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGtgttgt                        <  2:846908/151‑1 (MQ=255)
                                                                                                                            atatCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTa                       <  2:791829/151‑1 (MQ=255)
                                                                                                                             tatCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTa                       >  2:987467/1‑150 (MQ=255)
                                                                                                                               tCAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCTTGAGCAGGCGTGTTGTAACg                    >  4:57862/1‑151 (MQ=255)
                                                                                                                                cAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGc                   >  2:843852/1‑151 (MQ=255)
                                                                                                                                cAATCGCCAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGc                   >  4:347369/1‑151 (MQ=255)
                                                                                                                                      ccAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCg             <  4:439277/151‑1 (MQ=255)
                                                                                                                                       cAGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCgg            >  1:1217294/1‑151 (MQ=255)
                                                                                                                                        aGTGTGGTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGc           <  1:986054/151‑1 (MQ=255)
                                                                                                                                             ggTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCa      <  3:131114/151‑1 (MQ=255)
                                                                                                                                              gTCATAGCGTCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCTCCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATc       <  1:164489/149‑1 (MQ=255)
                                                                                                                                              gTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAc     <  2:709910/151‑1 (MQ=255)
                                                                                                                                              gTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAc     >  2:158813/1‑151 (MQ=255)
                                                                                                                                              gTCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAc     <  4:446122/151‑1 (MQ=255)
                                                                                                                                               tCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAcc    >  3:356774/1‑151 (MQ=255)
                                                                                                                                               tCATAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCAcc    >  2:743453/1‑151 (MQ=255)
                                                                                                                                                 aTAGCGCCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCg  <  2:1259079/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
CTAATGCGGATAAGGCATCACGCAAGGCTTCTGGTGTCTGGCTGGCTGGCGTAGGAAGTTCACGACGATCGCGGATCTGCCCGTCAGCGCCAATCAGCGCGGCGGCAAGTTTAGTACCGCCGATATCAATCGCCAGTGTGGTCATAGCACCGCCTTTTTCATCGCTGTGTTGTACCACTGACAAATGTGCTCAAGACGCGTGATTGCAGAACCGACCGTCACCGCCCACGCGCCGTGGCGCATCGCATCCGCCGCCTGAGCAGGCGTGTTGTAACGCCCTTCGGCAATCACCCG  >  NC_000913/3370206‑3370499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: