Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,410,937 G→A A220T (GCC→ACC)  dusB → tRNA‑dihydrouridine synthase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,410,9370GA99.2% 500.9 / NA 126A220T (GCC→ACC) dusBtRNA‑dihydrouridine synthase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (70/55);  minor base T (0/1);  total (70/56)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACT  >  NC_000913/3410789‑3411084
                                                                                                                                                    |                                                                                                                                                   
ccGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATAcc                                                                                                                                                   <  1:378764/151‑1 (MQ=255)
 cGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATAccc                                                                                                                                                  <  1:946265/151‑1 (MQ=255)
    acacGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCtg                                                                                                                                                <  3:257367/150‑1 (MQ=255)
       cgcgCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCtgatg                                                                                                                                             >  2:389521/1‑150 (MQ=255)
         cgcCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTgatgat                                                                                                                                           >  2:872010/1‑150 (MQ=255)
          gcCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAg                                                                                                                                         <  2:1094858/151‑1 (MQ=255)
           ccTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAgg                                                                                                                                        >  1:110646/1‑151 (MQ=255)
           ccTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAgg                                                                                                                                        <  3:125028/151‑1 (MQ=255)
           ccTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAgg                                                                                                                                        >  2:774789/1‑151 (MQ=255)
               tttgttCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCcgc                                                                                                                                    >  2:1022157/1‑151 (MQ=255)
                   ttCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCgcgc                                                                                                                                  >  4:20490/1‑149 (MQ=255)
                      aaTGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTc                                                                                                                             <  3:184182/151‑1 (MQ=255)
                         ggAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAgg                                                                                                                          <  1:845250/151‑1 (MQ=255)
                            gaAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGaa                                                                                                                       <  2:660603/151‑1 (MQ=255)
                             aaGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGa                                                                                                                        >  3:457397/1‑149 (MQ=255)
                             aaGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAg                                                                                                                      <  3:409295/151‑1 (MQ=255)
                               gCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGAc                                                                                                                    <  2:331955/151‑1 (MQ=255)
                                cTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGAcc                                                                                                                   >  2:461331/1‑151 (MQ=255)
                                  gAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCt                                                                                                                 <  1:28948/151‑1 (MQ=255)
                                  gAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCt                                                                                                                 <  1:389591/151‑1 (MQ=255)
                                     tACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGTGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTgg                                                                                                               >  1:69366/1‑150 (MQ=255)
                                     tACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTgg                                                                                                               >  1:344285/1‑150 (MQ=255)
                                     tACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTgg                                                                                                               >  2:1228957/1‑150 (MQ=255)
                                        gACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCt                                                                                                           >  1:733197/1‑151 (MQ=255)
                                        gACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCt                                                                                                           >  4:382388/1‑151 (MQ=255)
                                        gACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCt                                                                                                           <  1:419920/151‑1 (MQ=255)
                                        gACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCt                                                                                                           >  4:191166/1‑151 (MQ=255)
                                            gTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCg                                                                                                       >  4:456866/1‑151 (MQ=255)
                                            gTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCg                                                                                                       >  2:100767/1‑151 (MQ=255)
                                               ttCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCggg                                                                                                     >  2:462732/1‑150 (MQ=255)
                                                  gggCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAAt                                                                                                 <  1:675620/151‑1 (MQ=255)
                                                    gCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATcc                                                                                               >  2:539439/1‑151 (MQ=255)
                                                    gCAGTTAAGCAGAAAGGTTCCATTCCGGTTATCGCCAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGTAAAt                                                                                                 >  2:613871/1‑149 (MQ=255)
                                                     cAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCa                                                                                              <  1:1157155/151‑1 (MQ=255)
                                                      aGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATcc                                                                                               >  3:252432/1‑149 (MQ=255)
                                                      aGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCa                                                                                              <  2:924212/150‑1 (MQ=255)
                                                         tAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCAt                                                                                          >  1:51474/1‑151 (MQ=255)
                                                          aaGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCAtt                                                                                         <  1:605033/151‑1 (MQ=255)
                                                          aaGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCAt                                                                                          <  2:329406/150‑1 (MQ=255)
                                                           aGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCAtt                                                                                         >  4:281753/1‑150 (MQ=255)
                                                                aaaGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTgg                                                                                   <  1:1014719/151‑1 (MQ=255)
                                                                 aaGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTgg                                                                                   >  4:279423/1‑150 (MQ=255)
                                                                   gTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGaca                                                                                <  4:403732/151‑1 (MQ=255)
                                                                      tCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACt                                                                              <  2:662654/150‑1 (MQ=255)
                                                                      tCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACt                                                                              <  2:588885/150‑1 (MQ=255)
                                                                       ccATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTg                                                                             <  4:212619/150‑1 (MQ=255)
                                                                        cATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg                                                                           >  4:375282/1‑151 (MQ=255)
                                                                        cATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg                                                                           >  1:1036820/1‑151 (MQ=255)
                                                                        cATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg                                                                           >  4:195322/1‑151 (MQ=255)
                                                                         aTTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg                                                                          >  2:575482/1‑151 (MQ=255)
                                                                         aTTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg                                                                          <  2:306079/151‑1 (MQ=255)
                                                                         aTTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg                                                                          <  3:405992/151‑1 (MQ=255)
                                                                          ttCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg                                                                          >  1:778111/1‑150 (MQ=255)
                                                                             cGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt                                                                      <  1:475598/151‑1 (MQ=255)
                                                                                 tATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctg                                                                  >  2:129344/1‑151 (MQ=255)
                                                                                    cgcgAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTTGACACTGGGGAGTTGCTGcc                                                                <  2:1168529/150‑1 (MQ=255)
                                                                                    cgcgAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccc                                                               >  1:195490/1‑151 (MQ=255)
                                                                                      cgAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc                                                             >  2:460728/1‑151 (MQ=255)
                                                                                       gAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCg                                                            >  4:265853/1‑151 (MQ=255)
                                                                                       gAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCg                                                            >  1:540877/1‑151 (MQ=255)
                                                                                          tGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTg                                                         >  2:1089412/1‑151 (MQ=255)
                                                                                           ggCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTg                                                         <  2:865509/150‑1 (MQ=255)
                                                                                             cGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGcc                                                       <  1:1006665/150‑1 (MQ=255)
                                                                                             cGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt                                                      >  2:1043842/1‑151 (MQ=255)
                                                                                                      tGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCag                                               >  1:304956/1‑149 (MQ=255)
                                                                                                      tGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCag                                               >  3:218189/1‑149 (MQ=255)
                                                                                                       gACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCagag                                             <  2:142708/150‑1 (MQ=255)
                                                                                                               tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAgcg                                     <  3:424652/150‑1 (MQ=255)
                                                                                                                 aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgctt                                  >  1:851668/1‑151 (MQ=255)
                                                                                                                 aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgctt                                  <  4:252436/151‑1 (MQ=255)
                                                                                                                   gCCAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgcttgc                                >  1:233016/1‑151 (MQ=255)
                                                                                                                    ccAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgcttgct                               >  4:429737/1‑151 (MQ=255)
                                                                                                                     cAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgcttgctt                              >  2:1010681/1‑151 (MQ=255)
                                                                                                                     cAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgcttgctt                              >  4:107911/1‑151 (MQ=255)
                                                                                                                     cAGAGCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCgcttgctt                              <  4:106219/151‑1 (MQ=255)
                                                                                                                       gagCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTg                            >  4:366631/1‑151 (MQ=255)
                                                                                                                       gagCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTg                            <  2:985154/151‑1 (MQ=255)
                                                                                                                       gagCTGTGCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTg                            <  1:434186/151‑1 (MQ=255)
                                                                                                                       gagCTGTGCCCGACTATACAGGGGCGGATTCCCTGATGATAGGCCGCGCAGCTCAGGGAAGGCCCTGGATCTTTCGGGAAATTCAGCAATATCTGGGCACTTGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCttt                             <  3:245159/150‑1 (MQ=255)
                                                                                                                           tgtgCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTgcgcg                        >  3:395348/1‑151 (MQ=255)
                                                                                                                           tgtgCTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTgcgcg                        >  2:1229840/1‑151 (MQ=255)
                                                                                                                               cTCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACg                    >  1:1022805/1‑151 (MQ=255)
                                                                                                                                tCGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGt                   >  1:729522/1‑151 (MQ=255)
                                                                                                                                 cGACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACg                    <  1:524876/149‑1 (MQ=255)
                                                                                                                                  gACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTc                 <  4:349155/151‑1 (MQ=255)
                                                                                                                                  gACTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTc                 <  2:1125293/151‑1 (MQ=255)
                                                                                                                                   aCTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCg                <  1:119155/151‑1 (MQ=255)
                                                                                                                                   aCTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCg                >  1:749463/1‑151 (MQ=255)
                                                                                                                                   aCTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCg                <  2:69357/151‑1 (MQ=255)
                                                                                                                                    cTATACAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCgg               >  3:129325/1‑151 (MQ=255)
                                                                                                                                     tataCAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCggg              >  1:1071988/1‑151 (MQ=255)
                                                                                                                                     tataCAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCggg              >  2:204832/1‑151 (MQ=255)
                                                                                                                                       taCAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGTAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCg                <  2:413236/147‑1 (MQ=255)
                                                                                                                                         cAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACt          <  1:1122153/151‑1 (MQ=255)
                                                                                                                                         cAGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACt          <  1:343368/151‑1 (MQ=255)
                                                                                                                                          aGGGGCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTg         >  1:176992/1‑151 (MQ=255)
                                                                                                                                           ggggCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCGGGACACTGGGGAGTTGCTGCCCCCGCGGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTg         <  3:134022/150‑1 (MQ=255)
                                                                                                                                            gggCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCa       <  1:860995/151‑1 (MQ=255)
                                                                                                                                             ggCGGATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCAt      <  2:221374/151‑1 (MQ=255)
                                                                                                                                                ggATACCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGAc   >  2:266997/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGTACACGCGCCTGTTTGTTCAATGGAGAAGCTGAGTACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGGTTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGCAGAGGTTAAGCGCTTGCTTTGCGCGCACGTTCGGGAACTGCATGACT  >  NC_000913/3410789‑3411084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: