Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,590,692 T→C T74A (ACG→GCG)  ugpA ← sn‑glycerol‑3‑phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,590,6920TC99.1% 429.6 / ‑4.4 115T74A (ACG→GCG) ugpAsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (65/49);  total (65/50)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 118 total aligned reads displayed.

CGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATAAAAAAGATAACG  >  NC_000913/3590545‑3590837
                                                                                                                                                   |                                                                                                                                                 
cggcAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCa                                                                                                                                                <  2:967439/151‑1 (MQ=255)
    aaCGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAg                                                                                                                                            <  3:94822/151‑1 (MQ=255)
     aCGGCGGGAGCCCCGGCGGAAGGCGGCAAAATTTAGGGTTGATAGAAACGGCCGCCGCGCACCATGTACTCCCCCAGCGCCGCACAGGACAGCGAGACCAGCAAACCGCTGACGGTGAGAAAGGAGCTGAATTTTCTCGGCGCCCAGAAgg                                                                                                                                           >  3:340720/1‑151 (MQ=255)
        gcggGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGt                                                                                                                                        >  1:267562/1‑151 (MQ=255)
            gAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCgag                                                                                                                                    <  2:1100662/151‑1 (MQ=255)
                cACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGAt                                                                                                                                  >  3:350398/1‑149 (MQ=255)
                   ggCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGAtagta                                                                                                                              <  2:386289/150‑1 (MQ=255)
                    gCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGATATAGTAGc                                                                                                                            <  1:302106/151‑1 (MQ=255)
                    gCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGc                                                                                                                            <  3:206754/151‑1 (MQ=255)
                     cGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCt                                                                                                                           >  1:1062333/1‑151 (MQ=255)
                      gTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCt                                                                                                                           <  1:1052288/150‑1 (MQ=255)
                        aaGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGt                                                                                                                         <  1:419302/150‑1 (MQ=255)
                        aaGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTc                                                                                                                        >  1:243365/1‑151 (MQ=255)
                               caACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAAc                                                                                                                 <  2:307649/151‑1 (MQ=255)
                                  cATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGc                                                                                                              <  1:1069538/151‑1 (MQ=255)
                                      aaGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGa                                                                                                          <  4:255016/151‑1 (MQ=255)
                                       aGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGAc                                                                                                         <  1:1252500/151‑1 (MQ=255)
                                         gTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACg                                                                                                        >  3:340601/1‑150 (MQ=255)
                                         gTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACg                                                                                                        >  4:73682/1‑150 (MQ=255)
                                          tttGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGa                                                                                                       >  2:192116/1‑150 (MQ=255)
                                           ttGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGaa                                                                                                      <  2:130467/150‑1 (MQ=255)
                                           ttGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGaa                                                                                                      <  1:1048358/150‑1 (MQ=255)
                                           ttGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGaa                                                                                                      <  4:37802/150‑1 (MQ=255)
                                            tGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGt                                                                                                    >  2:844929/1‑151 (MQ=255)
                                             gATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGtt                                                                                                   <  3:167437/151‑1 (MQ=255)
                                             gATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGtt                                                                                                   <  1:496900/151‑1 (MQ=255)
                                             gATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCACTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGtt                                                                                                   <  2:1078560/151‑1 (MQ=255)
                                                  aaaCGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATcc                                                                                               >  2:967181/1‑150 (MQ=255)
                                                  aaaCGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATcc                                                                                               >  1:158791/1‑150 (MQ=255)
                                                   aaCGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGCGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATcc                                                                                               >  2:446670/1‑149 (MQ=255)
                                                    aCGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAgg                                                                                            >  2:1005471/1‑151 (MQ=255)
                                                     cGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGc                                                                                           >  1:775289/1‑151 (MQ=255)
                                                     cGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGc                                                                                           >  3:63868/1‑151 (MQ=255)
                                                     cGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGc                                                                                           >  1:1039611/1‑151 (MQ=255)
                                                          gCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGa                                                                                        <  1:781433/149‑1 (MQ=255)
                                                          gCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGAc                                                                                       <  3:316377/150‑1 (MQ=255)
                                                           ccGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACa                                                                                      >  4:389360/1‑150 (MQ=255)
                                                           ccGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACa                                                                                      >  4:265743/1‑150 (MQ=255)
                                                            cgcgCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACaa                                                                                     >  1:426431/1‑150 (MQ=255)
                                                             gcgcACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAAc                                                                                   <  2:866352/151‑1 (MQ=255)
                                                               gcACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActg                                                                                 >  2:1140153/1‑151 (MQ=255)
                                                               gcACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActg                                                                                 <  4:33843/151‑1 (MQ=255)
                                                                 aCGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActggc                                                                               >  2:1256484/1‑151 (MQ=255)
                                                                   gATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActggctg                                                                             >  2:686849/1‑151 (MQ=255)
                                                                   gATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActggct                                                                              <  1:453854/150‑1 (MQ=255)
                                                                    aTGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActggctgg                                                                            >  1:211830/1‑151 (MQ=255)
                                                                     tGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGCGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActggctg                                                                             >  2:115065/1‑149 (MQ=255)
                                                                      gTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAActggctgg                                                                            >  1:556112/1‑149 (MQ=255)
                                                                               caGCGCCGCAATGAACAGCGATACCAGCAACCCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAACGAGTCGAGATAGTAGCTATCAGGGAACAGCGTGACGAAGTTATCCAGGCAGACAAACTGGCTGGAGaac                                                                       <  2:416286/145‑2 (MQ=255)
                                                                                 gcgcCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACgg                                                               >  1:320257/1‑151 (MQ=255)
                                                                                  cgccgcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACgg                                                               >  4:170254/1‑150 (MQ=255)
                                                                                  cgccgcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGa                                                              <  2:1233564/151‑1 (MQ=255)
                                                                                  cgccgcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGa                                                              <  1:1222484/151‑1 (MQ=255)
                                                                                   gccgcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGAt                                                             <  1:673008/151‑1 (MQ=255)
                                                                                    ccgcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTTTCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATc                                                            >  1:26996/1‑151 (MQ=255)
                                                                                     cgcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCg                                                           >  3:196210/1‑151 (MQ=255)
                                                                                      gcAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCg                                                           >  4:197766/1‑150 (MQ=255)
                                                                                        aaaGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACg                                                        >  3:29431/1‑151 (MQ=255)
                                                                                        aaaGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACg                                                        >  1:1046006/1‑151 (MQ=255)
                                                                                           gAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCtt                                                     >  1:843643/1‑151 (MQ=255)
                                                                                            aaCAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATTGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCttt                                                    <  2:1151107/151‑1 (MQ=255)
                                                                                             aCAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTg                                                   >  2:1145715/1‑151 (MQ=255)
                                                                                              cAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGt                                                  >  1:495196/1‑151 (MQ=255)
                                                                                                     aCCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGt                                           >  2:1199358/1‑151 (MQ=255)
                                                                                                     aCCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGt                                           >  2:226955/1‑151 (MQ=255)
                                                                                                          cAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACcac                                      >  2:101265/1‑151 (MQ=255)
                                                                                                            aaCCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACcaca                                     >  2:203521/1‑150 (MQ=255)
                                                                                                             aCCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAAc                                   <  1:693668/151‑1 (MQ=255)
                                                                                                             aCCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAAc                                   >  1:521355/1‑151 (MQ=255)
                                                                                                                gCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCt                                >  2:815272/1‑151 (MQ=255)
                                                                                                                gCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCt                                >  4:354522/1‑151 (MQ=255)
                                                                                                                gCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCt                                >  2:358409/1‑151 (MQ=255)
                                                                                                                   gACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCg                             >  1:313105/1‑151 (MQ=255)
                                                                                                                   gACGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCg                             >  1:1137967/1‑151 (MQ=255)
                                                                                                                    aCGGTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGc                            <  1:476238/151‑1 (MQ=255)
                                                                                                                       gTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCg                         <  3:145196/151‑1 (MQ=255)
                                                                                                                       gTGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCg                         >  4:78012/1‑151 (MQ=255)
                                                                                                                        tGACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGc                        >  3:304077/1‑151 (MQ=255)
                                                                                                                         gACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGc                        <  2:1082436/150‑1 (MQ=255)
                                                                                                                         gACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGc                        <  2:305097/150‑1 (MQ=255)
                                                                                                                         gACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGc                        >  2:74901/1‑150 (MQ=255)
                                                                                                                         gACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCa                       <  4:340605/151‑1 (MQ=255)
                                                                                                                         gACAAAGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCa                       <  1:416832/151‑1 (MQ=255)
                                                                                                                            aaaGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGc                    >  2:1031167/1‑151 (MQ=255)
                                                                                                                              aGGTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCa                  <  3:170252/151‑1 (MQ=255)
                                                                                                                                gTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGcc                   >  2:338935/1‑148 (MQ=255)
                                                                                                                                gTGCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACACTTCGCCCGCAGGCCAGa                <  4:51032/151‑1 (MQ=255)
                                                                                                                                  gCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGAt               >  1:30247/1‑150 (MQ=255)
                                                                                                                                  gCTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGAGCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATa              <  4:303306/151‑1 (MQ=255)
                                                                                                                                   cTGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATa              >  1:583167/1‑150 (MQ=255)
                                                                                                                                    tGAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATaa             >  1:471264/1‑150 (MQ=255)
                                                                                                                                     gAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATaaaa           <  1:1134295/151‑1 (MQ=255)
                                                                                                                                     gAATTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATaaa            >  2:724160/1‑150 (MQ=255)
                                                                                                                                      aaTTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATaaaaa          <  2:587593/151‑1 (MQ=255)
                                                                                                                                      aaTTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATaaaa           >  1:234777/1‑150 (MQ=255)
                                                                                                                                      aaTTTTATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATaaaa           >  1:1221323/1‑150 (MQ=255)
                                                                                                                                          ttATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATAAAAAAGa       <  1:385090/150‑1 (MQ=255)
                                                                                                                                           tATCGTCGCCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATAAAAAAGAt      <  4:177552/150‑1 (MQ=255)
                                                                                                                                              cgtcgcCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACGGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATAAAAAAGATAACg  >  2:917014/1‑151 (MQ=255)
                                                                                                                                              cgtcgcCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATAAAAAAGATAACg  >  1:280407/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCCACCAGCGCCGCAAAGAACAGCGATACCAGCAAACCGCTGACGGTGACAAAGGTGCTGAATTTTATCGTCGTCCAGAAGGAGTCGAGATAGTAGCTGTCATGGAACAGCGTGACGAAGTTATCCAGGCCGACAAACTGGCTGGAGAAACCAAACGGATCGACGCTTTGTAGCGAGTACCACAACGCTTCGCCCGCAGGCCAGATAAAAAAGATAACG  >  NC_000913/3590545‑3590837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: