Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,684,183 A→G V479A (GTG→GCG)  yhjK ← cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,684,1830AG100.0% 482.5 / NA 125V479A (GTG→GCG) yhjKcyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (58/67);  total (58/67)

Only 100 of 125 total aligned reads displayed.

GGCTGAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCACCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGACCTGTGGCT  >  NC_000913/3684036‑3684330
                                                                                                                                                   |                                                                                                                                                   
ggCTGAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGccc                                                                                                                                                  <  2:804016/151‑1 (MQ=255)
ggCTGAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGccc                                                                                                                                                  <  2:302715/151‑1 (MQ=255)
    gAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGt                                                                                                                                               <  3:424138/150‑1 (MQ=255)
    gAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGt                                                                                                                                               <  1:897074/150‑1 (MQ=255)
    gAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTg                                                                                                                                              >  1:624338/1‑151 (MQ=255)
     aaTGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGa                                                                                                                                             <  3:185513/151‑1 (MQ=255)
        gCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCg                                                                                                                                          >  1:613969/1‑151 (MQ=255)
        gCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCg                                                                                                                                          <  2:1179765/151‑1 (MQ=255)
         cGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCg                                                                                                                                          >  3:415316/1‑150 (MQ=255)
          gATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGAc                                                                                                                                        >  2:269708/1‑151 (MQ=255)
           aTAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACg                                                                                                                                       <  4:365470/151‑1 (MQ=255)
             aGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGt                                                                                                                                     <  4:162862/151‑1 (MQ=255)
             aGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGt                                                                                                                                     >  2:903272/1‑151 (MQ=255)
              gCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGt                                                                                                                                     >  2:163720/1‑150 (MQ=255)
                  gTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAAcc                                                                                                                                 >  2:575903/1‑150 (MQ=255)
                    tAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCAACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCAt                                                                                                                               <  1:585175/150‑1 (MQ=255)
                     aaCAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCa                                                                                                                             <  2:2386/151‑1 (MQ=255)
                       cAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGc                                                                                                                           >  1:133697/1‑151 (MQ=255)
                           tCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCcac                                                                                                                       >  4:317169/1‑151 (MQ=255)
                             cAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAg                                                                                                                     <  3:306751/151‑1 (MQ=255)
                             cAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAg                                                                                                                     <  1:1250248/151‑1 (MQ=255)
                               gCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCa                                                                                                                   <  1:136896/151‑1 (MQ=255)
                                cATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCAc                                                                                                                  >  1:299890/1‑151 (MQ=255)
                                 atatCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACt                                                                                                                 >  1:1138048/1‑151 (MQ=255)
                                 atatCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACt                                                                                                                 >  2:1152942/1‑151 (MQ=255)
                                   atCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCACTCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTc                                                                                                                >  2:45545/1‑150 (MQ=255)
                                     ccGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAAt                                                                                                             <  1:796286/151‑1 (MQ=255)
                                        ccaccaTATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcg                                                                                                          >  2:950310/1‑151 (MQ=255)
                                         caccaTATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcg                                                                                                          >  1:910358/1‑150 (MQ=255)
                                           ccaTATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatc                                                                                                       >  2:467467/1‑151 (MQ=255)
                                            caTATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatcg                                                                                                      >  1:829481/1‑151 (MQ=255)
                                             atatTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGTGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatcg                                                                                                      >  3:52573/1‑150 (MQ=255)
                                             atatTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatcg                                                                                                      >  1:104835/1‑150 (MQ=255)
                                             atatTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatcg                                                                                                      >  2:515575/1‑150 (MQ=255)
                                              tatTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatcgat                                                                                                    >  2:66220/1‑151 (MQ=255)
                                               atTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGcgatcgat                                                                                                    <  2:829313/150‑1 (MQ=255)
                                               atTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGAtt                                                                                                   <  2:4082/151‑1 (MQ=255)
                                                  cGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAg                                                                                                <  3:305349/151‑1 (MQ=255)
                                                   gggTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGc                                                                                               <  2:1092427/151‑1 (MQ=255)
                                                        cATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATcc                                                                                          <  2:494716/151‑1 (MQ=255)
                                                        cATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATcc                                                                                          <  1:1112465/151‑1 (MQ=255)
                                                        cATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATcc                                                                                          <  3:114433/151‑1 (MQ=255)
                                                             gTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAg                                                                                     <  2:316655/151‑1 (MQ=255)
                                                                gCAGCGCAGAGAGGTTTACCGACAAGGGCAGCGTAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGTGATCGATTAAGCCATCCGGCAGGt                                                                                   <  4:215923/150‑1 (MQ=255)
                                                                gCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGt                                                                                   <  3:341040/150‑1 (MQ=255)
                                                                gCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTc                                                                                  <  4:265807/151‑1 (MQ=255)
                                                                gCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTc                                                                                  <  3:233412/151‑1 (MQ=255)
                                                                   gcgcAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCa                                                                               <  2:995449/151‑1 (MQ=255)
                                                                    cgcAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCaa                                                                              <  2:436676/151‑1 (MQ=255)
                                                                     gcAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAAc                                                                             >  2:893504/1‑151 (MQ=255)
                                                                     gcAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAAc                                                                             >  1:1063132/1‑151 (MQ=255)
                                                                      cAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACt                                                                            <  2:168922/151‑1 (MQ=255)
                                                                       agagagGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTg                                                                           >  4:213863/1‑151 (MQ=255)
                                                                       agagagGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTg                                                                           >  3:247830/1‑151 (MQ=255)
                                                                       agagagGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTg                                                                           >  1:422882/1‑151 (MQ=255)
                                                                        gagagGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGc                                                                          <  4:339514/151‑1 (MQ=255)
                                                                         agagGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGcc                                                                         <  1:1153152/151‑1 (MQ=255)
                                                                            ggTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATc                                                                      <  4:426757/151‑1 (MQ=255)
                                                                             gTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATcc                                                                     <  1:493014/151‑1 (MQ=255)
                                                                                tACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCgg                                                                   <  1:1079005/150‑1 (MQ=255)
                                                                                tACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGt                                                                  >  2:23490/1‑151 (MQ=255)
                                                                                 aCCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCCACTGCCATCCGGtt                                                                 >  1:499868/1‑151 (MQ=255)
                                                                                 aCCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGt                                                                  <  1:129112/150‑1 (MQ=255)
                                                                                  ccGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTg                                                                <  2:891505/151‑1 (MQ=255)
                                                                                   cGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGc                                                               >  1:335202/1‑151 (MQ=255)
                                                                                    gACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCt                                                              >  1:724706/1‑151 (MQ=255)
                                                                                     aCAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTg                                                             >  3:220428/1‑151 (MQ=255)
                                                                                      cAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTgg                                                            <  2:484145/151‑1 (MQ=255)
                                                                                      cAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTgg                                                            >  1:1055494/1‑151 (MQ=255)
                                                                                       aaGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTgg                                                            >  1:337803/1‑150 (MQ=255)
                                                                                        aGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGAt                                                          >  1:718167/1‑151 (MQ=255)
                                                                                         gggCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATa                                                         <  3:230685/151‑1 (MQ=255)
                                                                                             agcaTAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGc                                                      >  1:548027/1‑150 (MQ=255)
                                                                                             agcaTAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCa                                                     <  3:54078/151‑1 (MQ=255)
                                                                                               caTAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGt                                                   <  3:235405/151‑1 (MQ=255)
                                                                                                     gCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAacac                                              <  4:350607/150‑1 (MQ=255)
                                                                                                      ccGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACtt                                            >  2:843018/1‑151 (MQ=255)
                                                                                                         cgcTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCTCTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACCCTTCCg                                         <  3:30803/151‑1 (MQ=255)
                                                                                                          gcTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGc                                        >  4:183202/1‑151 (MQ=255)
                                                                                                           ctctTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGc                                        >  3:219931/1‑150 (MQ=255)
                                                                                                            tctTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCAc                                      >  1:659877/1‑151 (MQ=255)
                                                                                                              ttGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTg                                    >  2:704180/1‑151 (MQ=255)
                                                                                                                gCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGa                                   <  3:188438/150‑1 (MQ=255)
                                                                                                                 ccAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGAcc                                 >  3:149256/1‑151 (MQ=255)
                                                                                                                  cAGGCTGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCa                                <  1:1167248/151‑1 (MQ=255)
                                                                                                                       tGCAAGCAATCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGttt                            >  4:370444/1‑150 (MQ=255)
                                                                                                                         caagcaaTCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTAcc                         <  2:1073196/151‑1 (MQ=255)
                                                                                                                          aagcaaTCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCg                        <  1:576014/151‑1 (MQ=255)
                                                                                                                           agcaaTCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGc                       >  1:907285/1‑151 (MQ=255)
                                                                                                                             caaTCGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTg                     >  2:247476/1‑151 (MQ=255)
                                                                                                                                 cGACAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTc                  <  1:73715/150‑1 (MQ=255)
                                                                                                                                   aCAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCAtc               <  1:879916/151‑1 (MQ=255)
                                                                                                                                    cAGGACTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCAtct              <  1:696168/151‑1 (MQ=255)
                                                                                                                                        aCTCTTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGAc          <  4:437959/151‑1 (MQ=255)
                                                                                                                                           ctTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGAcc         <  3:146363/149‑1 (MQ=255)
                                                                                                                                           ctTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGAcc         <  2:35543/149‑1 (MQ=255)
                                                                                                                                           ctTCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGACCtg       >  1:402275/1‑151 (MQ=255)
                                                                                                                                             tCCAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGACCt        <  1:345305/148‑1 (MQ=255)
                                                                                                                                              ccAGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGACCTGTgg    <  2:403865/151‑1 (MQ=255)
                                                                                                                                                aGCGCCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGACCTGTGGCt  <  2:299844/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCTGAATGCGATAGCGGGTTAACAGTTCCAGCATATCCGCCACCATATTCGGGTGCATCAGTTGCAGCGCAGAGAGGTTTACCGACAAGGGCAGCATAATGCCGCGCTCTTGCCAGGCTGCAAGCAATCGACAGGACTCTTCCAGCACCCAGTGACCGACGGTAACCATCAGCCCACAGCACTCAATGCGATCGATTAAGCCATCCGGCAGGTCCCAACTGCCATCCGGTTGCTGGATACGCAGTAACACTTCCGCACTGACCAGTTTACCGCTGGTCATCTCGACCTGTGGCT  >  NC_000913/3684036‑3684330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: