Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,933	C→T	A329V (GCA→GTA)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,933	0	C	T	100.0%	351.7 / NA	92	A329V (GCA→GTA)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/49);  total (43/49)

ATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGCATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGTTTGTTGATATT  >  NC_000913/3797786‑3798080                                                                                                                                                    |                                                                                                                                                    aTAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTAtt                                                                                                                                                  <  1:630251/151‑1 (MQ=255)   aGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTgg                                                                                                                                                >  1:438237/1‑151 (MQ=255)    gtgtTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGa                                                                                                                                               <  1:855425/151‑1 (MQ=255)      gtTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGt                                                                                                                                             <  1:1129342/151‑1 (MQ=255)        tACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGtt                                                                                                                                            <  1:1177306/150‑1 (MQ=255)         aCTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGtttt                                                                                                                                          >  2:1016969/1‑151 (MQ=255)          cTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGtt                                                                                                                                            <  2:1238300/148‑1 (MQ=255)           ttCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCt                                                                                                                                        >  2:14386/1‑151 (MQ=255)              ccTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAAc                                                                                                                                     <  1:925608/151‑1 (MQ=255)                 aGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGt                                                                                                                                   <  2:796748/150‑1 (MQ=255)                 aGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGt                                                                                                                                   >  1:64372/1‑150 (MQ=255)                 aGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGt                                                                                                                                   <  2:1240812/150‑1 (MQ=255)                   gTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTAc                                                                                                                                 >  2:293459/1‑150 (MQ=255)                     gCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACAt                                                                                                                               <  1:614748/150‑1 (MQ=255)                     gCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACAt                                                                                                                               <  1:839192/150‑1 (MQ=255)                     gCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATc                                                                                                                              <  2:863861/151‑1 (MQ=255)                       aaGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCt                                                                                                                             >  3:204337/1‑150 (MQ=255)                        aGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTAc                                                                                                                           >  1:464636/1‑151 (MQ=255)                         gACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTAc                                                                                                                           >  1:204880/1‑150 (MQ=255)                          aCTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACAt                                                                                                                         <  1:404530/151‑1 (MQ=255)                           cTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACAt                                                                                                                         >  1:562588/1‑150 (MQ=255)                                   gTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATa                                                                                                                <  2:751811/151‑1 (MQ=255)                                    tACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATa                                                                                                                >  3:269088/1‑150 (MQ=255)                                    tACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATa                                                                                                                >  2:54924/1‑150 (MQ=255)                                     aCGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAAt                                                                                                              >  1:177099/1‑151 (MQ=255)                                      cGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAAtt                                                                                                             >  1:1048553/1‑151 (MQ=255)                                        aaaTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGa                                                                                                           <  1:576704/151‑1 (MQ=255)                                        aaaTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGa                                                                                                           <  2:1092705/151‑1 (MQ=255)                                        aaaTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGa                                                                                                           <  2:626289/151‑1 (MQ=255)                                         aaTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGaa                                                                                                          <  2:668338/151‑1 (MQ=255)                                         aaTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGaa                                                                                                          <  1:236760/151‑1 (MQ=255)                                          aTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAg                                                                                                         <  2:269239/151‑1 (MQ=255)                                          aTTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAg                                                                                                         <  3:18950/151‑1 (MQ=255)                                            tGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGc                                                                                                        >  2:155417/1‑150 (MQ=255)                                             ggTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCa                                                                                                       <  2:205616/150‑1 (MQ=255)                                                 tAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTc                                                                                                  >  1:960182/1‑151 (MQ=255)                                                      atatTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTg                                                                                              >  2:1250340/1‑150 (MQ=255)                                                      atatTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTg                                                                                              >  2:824256/1‑150 (MQ=255)                                                      atatTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTg                                                                                              >  2:1118867/1‑150 (MQ=255)                                                        atTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGaaa                                                                                           <  1:197981/151‑1 (MQ=255)                                                        atTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGaa                                                                                            <  2:763849/150‑1 (MQ=255)                                                           cATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGt                                                                                        <  1:861922/151‑1 (MQ=255)                                                             tAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCt                                                                                      >  1:411735/1‑151 (MQ=255)                                                              aaaGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTg                                                                                     >  2:47370/1‑151 (MQ=255)                                                                  tCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATgg                                                                                 >  2:596973/1‑151 (MQ=255)                                                                   cACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATgg                                                                                 <  2:446844/150‑1 (MQ=255)                                                                    aCCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGa                                                                               <  1:492618/151‑1 (MQ=255)                                                                           tCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAAtttttt                                                                        >  1:730001/1‑151 (MQ=255)                                                                            cATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAAtttttt                                                                        <  2:1153640/150‑1 (MQ=255)                                                                            cATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAAtttttt                                                                        <  2:278309/150‑1 (MQ=255)                                                                             aTTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAAtttttt                                                                        <  2:284054/149‑1 (MQ=255)                                                                             aTTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTcc                                                                      <  2:550580/151‑1 (MQ=255)                                                                                  gATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACt                                                                 >  1:812542/1‑151 (MQ=255)                                                                                     cagcagAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACtttt                                                              <  4:115661/151‑1 (MQ=255)                                                                                     cagcagAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACtttt                                                              >  2:40849/1‑151 (MQ=255)                                                                                         agagTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCctc                                                          >  2:577729/1‑151 (MQ=255)                                                                                         agagTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCctc                                                          >  2:805178/1‑151 (MQ=255)                                                                                           agTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCctct                                                         >  1:763086/1‑150 (MQ=255)                                                                                            gTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTAt                                                       <  1:254082/151‑1 (MQ=255)                                                                                               aCGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTAtttt                                                    >  2:652569/1‑151 (MQ=255)                                                                                                cgcgCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTAttttt                                                   >  1:1271950/1‑151 (MQ=255)                                                                                                  cgcTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTc                                                  >  2:315487/1‑150 (MQ=255)                                                                                                      gAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTAt                                             >  4:133432/1‑151 (MQ=255)                                                                                                       aaaGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTAtt                                            <  2:1271718/151‑1 (MQ=255)                                                                                                       aaaGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTAtt                                            <  2:1123792/151‑1 (MQ=255)                                                                                                         aGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTAtttt                                          >  1:742555/1‑151 (MQ=255)                                                                                                          gTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTAtttt                                          >  2:409243/1‑150 (MQ=255)                                                                                                             tGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTtatat                                      >  1:544285/1‑151 (MQ=255)                                                                                                              gAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTtatat                                      >  1:945876/1‑150 (MQ=255)                                                                                                               aaTTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAg                                    <  1:121990/151‑1 (MQ=255)                                                                                                                aTTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGc                                   <  2:60051/151‑1 (MQ=255)                                                                                                                  ttGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCa                                  <  2:149446/150‑1 (MQ=255)                                                                                                                  ttGTTAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCa                                  <  2:928834/150‑1 (MQ=255)                                                                                                                      tAGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCAtata                              <  1:1017163/150‑1 (MQ=255)                                                                                                                       aGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATaa                             >  2:697665/1‑150 (MQ=255)                                                                                                                       aGTTGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATaa                             <  1:117429/150‑1 (MQ=255)                                                                                                                          tGCAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGACTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATaaaaa                          >  3:357954/1‑150 (MQ=255)                                                                                                                            cAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACg                       <  1:857035/151‑1 (MQ=255)                                                                                                                            cAGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACg                       >  1:274588/1‑151 (MQ=255)                                                                                                                             aGAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGa                      <  1:616218/151‑1 (MQ=255)                                                                                                                              gAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACg                       >  2:1128204/1‑149 (MQ=255)                                                                                                                              gAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACg                       >  2:1211544/1‑149 (MQ=255)                                                                                                                              gAACACAATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAg                     <  1:837585/151‑1 (MQ=255)                                                                                                                               aaCACAATAGCCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAg                     <  1:781278/150‑1 (MQ=255)                                                                                                                                 cacaATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCtt                  >  1:201871/1‑151 (MQ=255)                                                                                                                                  acaATAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCttt                 <  2:628382/151‑1 (MQ=255)                                                                                                                                     aTAGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTggg              >  3:144016/1‑151 (MQ=255)                                                                                                                                       aGGCTAAGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGt             <  1:254033/150‑1 (MQ=255)                                                                                                                                         gCTAAGAGGAGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTATTCCTCTATTTTTCACTATTGTATATAGCATATAAAAAACGAGCTTTGGGTttg          <  2:209916/151‑1 (MQ=255)                                                                                                                                            aaGAGGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGTttgttg       >  1:442275/1‑151 (MQ=255)                                                                                                                                              gagGGGTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGTTTGTTGat     <  1:25793/151‑1 (MQ=255)                                                                                                                                                 gggTATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGTTTGTTGATAtt  <  2:646767/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAGTCACCTTTTTCATTTAGATCAGCAGAGTCACGCGCTGAAAGTATGAATTTGTTAGTTGCAGAACACAATAGGCTAAGAGGGGCATTGGAGTTTTCTAACGTACATCTACATAATGAGATAATTGAAGCAGGGTCACTGAAAGGTCTGATGGGAATTTTTTCCACACTTTTCCTCTATTTTTCACTATTTTATATAGCATATAAAAAACGAGCTTTGGGTTTGTTGATATT  >  NC_000913/3797786‑3798080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑