Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,737,713 (G)6→7 intergenic (+67/‑32) pheL → / → pheA pheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,737,7071.G98.4% 226.7 / ‑2.5 63intergenic (+61/‑38)pheL/pheApheA gene leader peptide/chorismate mutase and prephenate dehydratase, P‑protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (34/28);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

AACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAGGTG  >  NC_000913/2737557‑2737849
                                                                                                                                                       |                                                                                                                                               
aaCAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATc                                                                                                                                                  <  1:719301/151‑1 (MQ=255)
aaCAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATc                                                                                                                                                  >  2:230271/1‑151 (MQ=255)
 aCAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCg                                                                                                                                                >  2:221520/1‑151 (MQ=255)
  cAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCgg                                                                                                                                               <  2:212379/151‑1 (MQ=255)
    gTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCgggg                                                                                                                                            <  1:526602/151‑1 (MQ=255)
      aCGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCgggggg                                                                                                                                          <  2:474/151‑1 (MQ=255)
      aCGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCggggg                                                                                                                                           <  1:549085/150‑1 (MQ=255)
       cGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCggggggg                                                                                                                                         <  1:238899/151‑2 (MQ=255)
          gtactgtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCgggggggcct                                                                                                                                      <  2:613952/151‑5 (MQ=255)
            actgtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCgggggggcctt                                                                                                                                     <  2:184099/150‑6 (MQ=255)
            actgtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCgggggggcctt                                                                                                                                     <  1:801479/150‑6 (MQ=255)
               gtactAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGTATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCtttttt                                                                                                                                  <  2:604754/151‑1 (MQ=255)
                   tAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTg                                                                                                                              >  2:1035490/1‑151 (MQ=255)
                       gTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATa                                                                                                                           >  1:15089/1‑150 (MQ=255)
                        tCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAAc                                                                                                                         >  2:296943/1‑151 (MQ=255)
                        tCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAAc                                                                                                                         >  2:298428/1‑151 (MQ=255)
                        tCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAAc                                                                                                                         >  1:125178/1‑151 (MQ=255)
                        tCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAAc                                                                                                                         >  2:39750/1‑151 (MQ=255)
                          aCTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACa                                                                                                                        >  2:1013871/1‑150 (MQ=255)
                          aCTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACa                                                                                                                        >  1:523105/1‑150 (MQ=255)
                             tAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACaaaa                                                                                                                     >  1:1033775/1‑150 (MQ=255)
                              aaGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCTTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACaaaaa                                                                                                                    <  2:330011/150‑1 (MQ=255)
                               aGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAgg                                                                                                                  <  2:485362/151‑1 (MQ=255)
                                  aaacaaacATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCaa                                                                                                               <  2:1172283/151‑1 (MQ=255)
                                   aacaaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAac                                                                                                              <  2:171894/151‑1 (MQ=255)
                                      aaacaTGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACt                                                                                                           <  2:1018928/151‑1 (MQ=255)
                                                cacaTACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCgg                                                                                                 >  2:1007411/1‑151 (MQ=255)
                                                          ttttCTTCGCATTCTTTTTTACGTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGtt                                                                                       >  1:798612/1‑151 (MQ=255)
                                                           tttCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGtt                                                                                       <  2:773587/150‑1 (MQ=255)
                                                           tttCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGtt                                                                                       >  2:483567/1‑150 (MQ=255)
                                                            ttcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTa                                                                                      <  1:127292/150‑1 (MQ=255)
                                                            ttcttcGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTa                                                                                      <  1:392167/150‑1 (MQ=255)
                                                                  gCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGgcgc                                                                               >  1:342215/1‑151 (MQ=255)
                                                                    aTTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTg                                                                             >  1:996454/1‑151 (MQ=255)
                                                                      tCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCg                                                                           >  1:137178/1‑151 (MQ=255)
                                                                         tttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCg                                                                           >  1:255563/1‑148 (MQ=255)
                                                                          ttttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGAGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCgag                                                                         >  2:619923/1‑149 (MQ=255)
                                                                            ttACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCgagaga                                                                      <  1:777693/150‑1 (MQ=255)
                                                                              aCCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGaaaa                                                                   <  2:366574/151‑1 (MQ=255)
                                                                              aCCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGaaa                                                                    >  2:1165152/1‑150 (MQ=255)
                                                                               ccTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGaaaa                                                                   <  1:925080/150‑1 (MQ=255)
                                                                               ccTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAAt                                                                  <  2:735418/151‑1 (MQ=255)
                                                                                 ttCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATc                                                                 >  1:220986/1‑150 (MQ=255)
                                                                                  tCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATc                                                                 <  2:209919/149‑1 (MQ=255)
                                                                                  tCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCa                                                                <  1:487558/150‑1 (MQ=255)
                                                                                  tCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGAGAGAGAAAATCa                                                                <  1:1167134/150‑1 (MQ=255)
                                                                                    cccTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcg                                                             >  2:277609/1‑151 (MQ=255)
                                                                                     ccTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgc                                                            >  1:1072588/1‑151 (MQ=255)
                                                                                       tGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAgcgcgc                                                          >  2:280843/1‑151 (MQ=255)
                                                                                         aaTGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTg                                                        <  1:514184/151‑1 (MQ=255)
                                                                                                ggCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaaa                                                 <  2:91291/151‑1 (MQ=255)
                                                                                                  cGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGaaaaa                                               <  2:714799/151‑1 (MQ=255)
                                                                                                      tcgtcgTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCTAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAattat                                           >  1:891600/1‑151 (MQ=255)
                                                                                                        gtcgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAAttatta                                         <  1:221517/151‑1 (MQ=255)
                                                                                                          cgtGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGc                                       >  1:318944/1‑151 (MQ=255)
                                                                                                             gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGt                                     <  1:17868/150‑1 (MQ=255)
                                                                                                             gtgAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGt                                     <  1:296940/150‑1 (MQ=255)
                                                                                                               gAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTAc                                  <  2:620975/151‑1 (MQ=255)
                                                                                                                aaaCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACt                                 <  2:338825/151‑1 (MQ=255)
                                                                                                                aaaCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACt                                 <  2:703937/151‑1 (MQ=255)
                                                                                                                 aaCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTg                                <  1:711266/151‑1 (MQ=255)
                                                                                                                  aCAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTgg                               >  2:1057669/1‑151 (MQ=255)
                                                                                                                   cAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGc                              <  2:799662/151‑1 (MQ=255)
                                                                                                                    aGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGc                              >  2:251468/1‑150 (MQ=255)
                                                                                                                     gAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGc                              >  1:87706/1‑149 (MQ=255)
                                                                                                                        tGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAAc                         >  1:848911/1‑151 (MQ=255)
                                                                                                                         gCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAAc                         <  2:437088/150‑1 (MQ=255)
                                                                                                                         gCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACg                        >  2:15605/1‑151 (MQ=255)
                                                                                                                              gACGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGcgcg                   >  2:1187789/1‑151 (MQ=255)
                                                                                                                                cGAACAATAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGa                  >  1:89177/1‑150 (MQ=255)
                                                                                                                                     aaTAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGc            >  1:1185818/1‑151 (MQ=255)
                                                                                                                                       tAAGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCg          >  2:90296/1‑151 (MQ=255)
                                                                                                                                        aaGGCCTCCCAAATCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTAGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGt         >  1:1023262/1‑151 (MQ=255)
                                                                                                                                                  aaaTCGGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGCAAAATTATTAGCGTTACTGGCAGAACGGCGCGAAATGGCCGTCGAGGTg  >  2:1114849/1‑148 (MQ=255)
                                                                                                                                                       |                                                                                                                                               
AACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAAGGCAACACTATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCGTTACTGGCAGAACGGCGCGAACTGGCCGTCGAGGTG  >  NC_000913/2737557‑2737849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: