Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	646,747	0	T	G	50.6%	106.0 / 134.7	78	T255P (ACC→CCC)	citG	2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base T (4/34);  new base G (39/0);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 9.07e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-15

ATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCT  >  NC_000913/646602‑646892                                                                                                                                                   |                                                                                                                                                    atTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGaa                                                                                                                                                <  1:252036/151‑1 (MQ=255)   ttCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑t                                                                                                                                              <  2:817065/150‑1 (MQ=255)    tCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑Tg                                                                                                                                             >  1:637280/1‑150 (MQ=255)     ccAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑c                                                                                                                                           <  1:23785/150‑1 (MQ=255)      cAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑ccc                                                                                                                                         <  2:1015214/151‑1 (MQ=255)      cAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑ccc                                                                                                                                         <  1:249134/151‑1 (MQ=255)       aaaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑cccc                                                                                                                                        >  1:778125/1‑151 (MQ=255)         aTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGA‑TA‑ccccc                                                                                                                                       >  2:706850/1‑150 (MQ=255)         aTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑ccccc                                                                                                                                       >  2:586500/1‑150 (MQ=255)          tttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑ccccc                                                                                                                                       >  2:129865/1‑149 (MQ=255)           ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAA‑AG‑GCCCCttt                                                                                                                                    >  2:755870/1‑151 (MQ=255)            tATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑CCCCCcttt                                                                                                                                   >  2:127290/1‑151 (MQ=255)             aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TG‑CCCCCctttt                                                                                                                                  >  2:1027055/1‑151 (MQ=255)              tCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TG‑CCCCCctttt                                                                                                                                  >  1:218492/1‑150 (MQ=255)               cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGTGGAT‑TG‑CCCCCttt                                                                                                                                    >  2:874282/1‑148 (MQ=255)                 aGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGA‑TG‑CCCCCctttt                                                                                                                                  >  1:355606/1‑147 (MQ=255)                 aGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAATTG‑CCCCCttttt                                                                                                                                  >  2:1088190/1‑148 (MQ=255)                       tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCaataat                                                                                                                          <  2:797492/149‑1 (MQ=255)                        aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGtt                                                                                                                       <  1:302690/151‑1 (MQ=255)                        aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGt                                                                                                                        <  1:508570/150‑1 (MQ=255)                        aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGt                                                                                                                        <  1:962384/150‑1 (MQ=255)                         aataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAA‑AG‑CCCCCCTTTTTCaataaatttt                                                                                                                      >  2:539708/1‑146 (MQ=255)                         aataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑CCCCCCTTTTTCCATAATGttt                                                                                                                      >  2:224905/1‑151 (MQ=255)                            aatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTgcg                                                                                                                   <  2:82175/151‑1 (MQ=255)                            aatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TA‑CCCCCCTTTTTTTATAATGt                                                                                                                        >  2:732980/1‑146 (MQ=255)                             atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTgcgc                                                                                                                  <  2:1000111/151‑1 (MQ=255)                               tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGcc                                                                                                                 <  2:576104/150‑1 (MQ=255)                                aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAA‑TG‑CCCCCCTTTTGCAATAATGtttttgcccc                                                                                                               >  2:858610/1‑144 (MQ=255)                                 aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTcg                                                                                                              <  2:786130/151‑1 (MQ=255)                                 aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAA‑TG‑CCCCCTTTTTTCAATAATTTTTTTGCCTc                                                                                                               >  2:1079567/1‑150 (MQ=255)                                 aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑CCCCCCTTTTTCAATAATGTTTTCGcc                                                                                                                 >  2:1000626/1‑148 (MQ=255)                                 aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑CCCCCCTTTTTCAATAAAGtt                                                                                                                       >  2:289800/1‑142 (MQ=255)                                  aTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGCTTGGA‑TG‑CCCCCCTTTTTCAATAATTTTTGAGcccc                                                                                                               >  1:908117/1‑147 (MQ=255)                                      gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑CCCCCCTTTTTTAATAATGTTTTCGCCCCgcgc                                                                                                           >  2:156764/1‑149 (MQ=255)                                        gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTa                                                                                                       <  2:653612/151‑1 (MQ=255)                                           aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TG‑CCCCCCTTTTTGAATAATGTTTTGGCCCCCCGCTGTAGcc                                                                                                    >  1:636360/1‑151 (MQ=255)                                           aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGCGGGTCGGA‑AG‑CCCCCCTTTTTTCATAATGATTTCGccccccggcgttg                                                                                                      >  1:1109461/1‑138 (MQ=255)                                            aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCa                                                                                                   <  1:125249/151‑1 (MQ=255)                                              aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TG‑CCCCCCTTTTTCAATAATATTTTTGCCCCCCCCCGTAGcc                                                                                                    >  1:771233/1‑148 (MQ=255)                                              aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑AG‑CCCCCCTTTTGTCATAAAGTTTTCGCCTCGCCCCGTAGcc                                                                                                    >  1:484882/1‑148 (MQ=255)                                                         gATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcccccc                                                                                      <  1:1125716/151‑1 (MQ=255)                                                          aTCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCt                                                                                     <  1:489545/151‑1 (MQ=255)                                                            cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGTGCAGGCCCCCCTc                                                                                    <  1:453209/150‑1 (MQ=255)                                                                aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑AG‑CCCCCCTTTTGCAATAAAGTTTTCGCCCCGCGCTGGAGCCAGCGCAGGccccccccccccc                                                                               >  1:132016/1‑144 (MQ=255)                                                                 ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATAGGC‑GGGGGGTCGAA‑TG‑ACCCCTTTTTTCAAAAATTTTTGGGCCTCGCGCGGTGGCCAGAGCAGGccccccctcgcaac                                                                              >  2:233406/1‑143 (MQ=255)                                                                       cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGa                                                                        <  2:218495/151‑1 (MQ=255)                                                                       cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTAGGA‑TG‑CCCCCCTTTTTCTATAATATTTTCGCCCCCCGCGGTAGCCAGCGCCGGGCCCCCCCGCcccc                                                                              >  1:265821/1‑145 (MQ=255)                                                                        aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGAt                                                                       <  2:121082/151‑1 (MQ=255)                                                                                  ggACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTgg                                                             <  2:400658/151‑1 (MQ=255)                                                                                  ggACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTg                                                              <  1:886173/150‑1 (MQ=255)                                                                                      tGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGCA‑TG‑CCCCCCTTTTTCAAAAATGTTTTCGCCTCGCGGCGTAGCCAACGCAGGCCCCCCCCCCCACCCCGCGACGCAACGGTgg                                                             >  1:173661/1‑147 (MQ=255)                                                                                       gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATc                                                         <  1:672734/150‑1 (MQ=255)                                                                                             tcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TG‑CCCCCCTTTTTCAATAAAATTTTCGCCCCCCGCCGTAGCCCGCGCCGGGCCCCCCCCCCACCCCGCGCCGGCACGTTGGGATCTcccgt                                                   >  1:1053839/1‑147 (MQ=255)                                                                                              cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa                                                 <  2:444165/151‑1 (MQ=255)                                                                                               gttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt                                                <  1:890354/151‑1 (MQ=255)                                                                                                   gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTTAA‑TT‑CCCCCCTTTTTGAAAAAAATTTTCGCCCCCCGCCGTATCCAACGCCGGCCCCCCCCCCCCCCCCCCCATGCAAAAGTGGGATTGCCGTTGGTTGcc                                            >  2:498309/1‑151 (MQ=255)                                                                                                      acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAA‑TG‑CCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCGCGGTGCAACGGTGGGATTGCCCTTGGTCGcc                                            >  1:653366/1‑148 (MQ=255)                                                                                                       cacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCa                                        <  1:325641/151‑1 (MQ=255)                                                                                                        acTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAg                                       <  1:167425/151‑1 (MQ=255)                                                                                                        acTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAA‑TG‑CCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGCGGCAACGCTGGGATCGCCGGTGATCGCCCTc                                         >  1:570635/1‑149 (MQ=255)                                                                                                          tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TT‑CCCCCCTTTTTCAATAATGTTTTTGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGGATTGCCGGTGATCGCCCTCAGt                                      >  2:592418/1‑150 (MQ=255)                                                                                                           cccTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                      <  2:507363/149‑1 (MQ=255)                                                                                                            ccTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑AG‑CCCCCCTTTTTCAAAAAAATTTTCGCCCCGCGCTGTCGCCACCGCCAGCCCCCCCCCCCCCCCCGCGATGCAACGGTGGGTTCGCCCGTGATCGccc                                           >  1:287193/1‑142 (MQ=255)                                                                                                              tGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGA‑TGCCCCCCTTTTTGC‑ATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCGCCCCCGCCCGGAGCAACGGTGGTATCGCCGGTGATCGCCATCCGTAgg                                   >  2:645115/1‑149 (MQ=255)                                                                                                               gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGa                                  <  2:227838/149‑1 (MQ=255)                                                                                                                tCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑CCCCCCTTTTTTCATAAAGTTTTCGCCCCGCGCTGTAGCCAGCGCAGGGCCCCCCCGCCCCCCCGCGGAGCCACGTTGGTATCGCCGGTGGTCGCCCTCAGTAgg                                   >  2:925571/1‑147 (MQ=255)                                                                                                                 cGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGA‑TT‑CCCCCTTTTTTCTATAATTTTTTCCCCCCCCGCTGGAGCCAGCGCAGGGCCCCCCCGCCACCGCGCGGTGCAACGTTGGGATTGCCGGTGGTCGCCCCCAGTAGGAg                                 >  2:1189083/1‑148 (MQ=255)                                                                                                                        ccGGAGATAATCGAGATCGGC‑GGGGGGTCGAA‑TG‑ACCCCCTTTTGAAATAATGTTTTCGCCTCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCGCGAAGCAAAGCTGGGATCGCCGTTTATCGCCATCCGTAGGGGCaaag                            >  2:699224/1‑144 (MQ=255)                                                                                                                         cGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑GCCCCCTTTTTCAATAATGTTTTTGCCCCGCGCTGTTACCAACGCCAGCCCCCCCCCCCACCCCCCGATGCAACCTTGGTATCGCCGTTTTTCGCCCTCCGTCGGAGCCAGGGAt                         >  1:1134824/1‑148 (MQ=255)                                                                                                                            agaTAATCGAGATCGGC‑GGGGGGTCGGA‑TG‑CCCCCCTTTTTTACTAAAATTTTTGCCCCGCCCTGTAGCCCGCCCCGGCCCCCCCCCCCCCCCCCCGATGCCACGACGGTATCGCCCGTTATTGCCACTAGTAGGAGCCAgg                            >  1:910040/1‑142 (MQ=255)                                                                                                                                aaTCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATg                <  1:32278/150‑1 (MQ=255)                                                                                                                                       aTCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa        <  1:458838/151‑1 (MQ=255)                                                                                                                                         cggc‑ggGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAgg      >  2:993125/1‑151 (MQ=255)                                                                                                                                         cggc‑ggGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAg       <  1:1079664/150‑1 (MQ=255)                                                                                                                                          ggc‑ggGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGGATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAgg      >  2:77981/1‑150 (MQ=255)                                                                                                                                            c‑ggGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATc   <  1:1043611/151‑1 (MQ=255)                                                                                                                                              gggggTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCt  <  2:531266/151‑1 (MQ=255)                                                                                                                                              gggggTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCt  >  2:98887/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                    ATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAA‑TG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCT  >  NC_000913/646602‑646892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑