| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,433 | 0 | G | C | 52.4% | 2.8 / 33.5 | 22 | intergenic (+604/+132) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base G (1/9); new base C (11/0); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04 | |||||||||||
CGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGT > NC_000913/1097296‑1097487 | cGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa < 2:492877/151‑1 (MQ=33) gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAc < 2:673765/151‑1 (MQ=33) aCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCCTCGCTCGCCCCCCGGGCAACCCCCTCCCTGCGTTCAc > 2:699032/1‑149 (MQ=11) ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCCCTTCGCCCGCCCCGCGGGCAGCCCCCCCACTGCGGTCAc > 2:1182637/1‑148 (MQ=11) cccGGTCGGTGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACggc < 1:473360/150‑2 (MQ=14) gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCTTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTc < 1:799033/151‑1 (MQ=14) gggggggctgggccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCt < 1:381043/133‑1 (MQ=255) ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCCTCGCTCCCCCTGCGGGCAACCCCCTCCCTGCGTTCCCGGCCTGTcc > 2:786976/1‑147 (MQ=11) ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAGTGACTCGCCTCGCTCGCCCCCCGGGCAGCCCCCTCCCCGCGTTCACGGCCTGTccca > 2:420351/1‑147 (MQ=11) ggCTTCTCAT‑CCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAGTGAATCCCCTCGCTCCCCCCGCGGGCAACCCCCTCCCCGCGTTCACGGTCTTTCCCAcc > 1:563221/1‑149 (MQ=11) gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTg < 2:16246/151‑1 (MQ=14) ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCCCTTCGCCCTCCCTCGGGGGAGCCCTCTTAATGCGTTCGCGGCCGGGCCAAccg > 1:964869/1‑147 (MQ=11) tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGATTTAATCTCCTTCCCCCCCCTGCTGGGCGCCCCCTCCCTGCgggtttccgctttcc > 1:1146318/1‑129 (MQ=2) aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCCCCTCCCCCGCCCCCCGGGCAACCCCCCCCCTTCGTTCCCGGCCCGTCCCACTgg > 2:1002250/1‑145 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑Gaa < 2:724423/150‑1 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑Gaa < 2:565142/150‑1 (MQ=11) gtgCAATATACGAAAAAAAAGCCCGGTCTGTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGGCTGTCGCCAGTTGTC‑Gaa < 2:229339/150‑1 (MQ=2) tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGAATCGCTTCGCTCGCCCCGCGGGGCGCCCCCTCCCTGCGTTTACCGGCTGTCCAACTGGCTTTCGCCCGTTTTC‑GAAcc > 2:838476/1‑151 (MQ=11) gCAATATACGAAAAAAAAGCCCGTACTTTCGTATGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCCCGCCCTGCGGGCAGCCCCCCCACCGCGTTCACGGGCTGTCCAACTGGCTGTCGCCCGGTGTC‑GAAccc > 2:1012801/1‑151 (MQ=11) gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCGCTTCCCCCGCCCTGCGGGCAGCCCCCTCCCTGCGGTCACGGCCTGTCCAACTCGCTGTCGCCCGTTGTC‑GAAccc > 1:296483/1‑151 (MQ=11) atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGAATCGCTTTGCTCGCCCCGCGGGCAGCCCCCCCACTGCGTTCACGGCCTGTCCAACCGGCTGTCGCCAGTTGTC‑GAACCCCgg > 2:705862/1‑151 (MQ=11) taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTACTCCCCTCCCTCGCCCCGCGGGGAGGCCGGTCGGTGGGGTGCCGGGCCGTCCCACTGGCTGTCGGCAGGTGTCGGAACCCCggc > 1:76993/1‑149 (MQ=2) | CGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTC‑GAACCCCGGT > NC_000913/1097296‑1097487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |