Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,056	0	G	A	51.4%	58.5 / 113.9	72	intergenic (‑119/+84)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (32/3);  new base A (0/37);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 2.29e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-14

CCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA  >  NC_000913/1824907‑1825202                                                                                                                                                      |                                                                                                                                                    ccGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag‑a                                                                                                                                                   <  1:748248/151‑1 (MQ=255)  cGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag‑aa                                                                                                                                                  <  2:144829/151‑1 (MQ=255)  cGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag‑aa                                                                                                                                                  <  1:311707/151‑1 (MQ=255)   gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag‑aa                                                                                                                                                  >  1:667029/1‑150 (MQ=255)   gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagag‑aa                                                                                                                                                  >  2:68722/1‑150 (MQ=255)     cATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGag‑aaaag                                                                                                                                               <  2:2890/151‑1 (MQ=255)         ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGag‑aaaagaaa                                                                                                                                            >  1:368064/1‑150 (MQ=255)           aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAAcc                                                                                                                                         >  1:896416/1‑151 (MQ=255)             gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAAccgc                                                                                                                                       >  2:1013068/1‑151 (MQ=255)              cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAAccgcc                                                                                                                                      <  1:105202/151‑1 (MQ=255)              cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAAccgcc                                                                                                                                      >  2:1166289/1‑151 (MQ=255)              cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAAccgcc                                                                                                                                      >  2:21516/1‑151 (MQ=255)                    aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCt                                                                                                                                >  1:127258/1‑151 (MQ=255)                      aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGt                                                                                                                              >  1:621532/1‑151 (MQ=255)                         aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTcc                                                                                                                            >  2:640601/1‑150 (MQ=255)                            ccATCCTCGCTCCCGCCCCGTTTTTTTCTTTTTTATCGTTCAGTTACCGGTGGATGGTTATCGGCACCTGGGGTGTTTTACGTTAGTGGTGATCAGTTTTTTTTTACAACTTTTTGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCg                                                                                                                        <  2:477883/151‑1 (MQ=255)                             cATACTCGCTCCCGGCCCGTTTTTTTCATTGTTATCTTTCAGGTAACCGTGGATGGTTATCGGCAACTGGGTTGATTTACTTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAAAAGAA‑AAAAGAAAACCGCCGATCCTGTc                                                                                                                             <  2:431057/145‑1 (MQ=255)                              aTACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                       >  1:11965/1‑150 (MQ=255)                                   cGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                 >  1:603138/1‑151 (MQ=255)                                   cGCTCCCGCCCCGTTTTTTTCTTTTTTATCTTTAGGGTACCCGTGGATGGTTAGCGGAACCGGGGGAAATTTACATTAGTGGTTAGCAGTTTTTTTTTCCATGTTTTAGAGAAGAA‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                 <  1:772609/151‑1 (MQ=255)                                       cccGGCCCGTTTTTTTCATTTTTATCGTTCAGTTAACCTTGGATGGTTTTCGGCACCTCGGGTGTTTTACTTTGGTGGGGATCAGTTTTTTTTTCCAACTGTTGGAGAAGAA‑AAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                              <  1:164248/150‑1 (MQ=255)                                            cccTTTTTTTTCATTGTTATCTTTGAGGTACCGGTGATTGTTTAGCGGCAACTGGGGTATTTTCCGTTGGTGGTGATCAGTTTTTTTTTACACCTGTTGGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                           <  1:1071013/148‑1 (MQ=255)                                                tttttttCTTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                      <  2:808151/146‑1 (MQ=255)                                                   ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                  >  2:664887/1‑150 (MQ=255)                                                        aTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                            >  1:347003/1‑151 (MQ=255)                                                        aTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                            >  1:454731/1‑151 (MQ=255)                                                            ttATCGTTCAGGTACCGGGGAATGGTTACCGGAACCTGGGGTGATTTCCGTTAGTGGTGATCAGGTTTTTTTTAAAACTTTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                          <  2:597568/149‑1 (MQ=255)                                                            ttATCGTCCAGTTACCCGGGGATGTTTATCGGAAACTGGGGTGTTTTAGGTTAGTGGTGATCGGGTTTTTTTTACAATTGTTGAAAAAAAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                          <  2:1092497/149‑1 (MQ=255)                                                              aTCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                      >  1:545328/1‑151 (MQ=255)                                                                   tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                    >  1:734871/1‑148 (MQ=255)                                                                   tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                   >  2:82829/1‑149 (MQ=255)                                                                      ggTAACCGTGGATGGTTATCGGAAACTGGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTTTTTGAGAAAAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                               <  2:999767/150‑1 (MQ=255)                                                                       gTACCCGTGGATGGTTATCGGCACCTGGGGTGATTTACGTTAGGGGTGATCAGTTTTTTTTTACACCTTTTTGAGAAAAA‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                 <  2:562233/147‑1 (MQ=255)                                                                       gTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                             >  2:707514/1‑151 (MQ=255)                                                                         accggTGGTTGGTTATCGGCAACTGGGGTGTTTTACTTTAGGGGTGATCAGGTTTTTTTAACAATTTTTGGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                <  1:1173592/142‑1 (MQ=255)                                                                         acccGTGGATTGTTACCGGAAACGGGGGTAATTTACGTTAGTGGTATCCAGGTTTTTTTTAAAACTGTTTGGAAAGAA‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                               <  2:339467/145‑1 (MQ=255)                                                                         acccGTGGATGGTTACCGCCACCGCGGGTAATTTACGTAAGTGGGGATCGGATTTTTTTAACACGTTTGTGAAAAGAA‑AAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                             <  1:43892/147‑1 (MQ=255)                                                                         aacgggggttGGTTATCGGCACCTGGGTTGATTTCCGTTGGTGGGAACCAGGTTTTTTTTCCAATTGTTGGAGAAGAA‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                             <  2:38887/140‑1 (MQ=255)                                                                         aaCGGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGGGGTAATCAGGTTTTTTTTACAACTGTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                             <  2:939177/149‑1 (MQ=255)                                                                         aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                             >  1:939158/1‑149 (MQ=255)                                                                                  tGGTTATCGCAAATTGGGGTTTTTTACGTTAGTGGTGATCGGGTTTTTTTAAAAATTTTTTGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                    <  1:1166260/149‑1 (MQ=255)                                                                                     ttATCGGCACCTCGGGTGATTTACGTTAGGGGTGATCAGGTTTTTTTTACAACTGTTGGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                <  2:1101229/150‑1 (MQ=255)                                                                                       aTCGGCACCTCGGGTGTTTTACGTTAGTGGTGATAAGGTTTTTTTTCCACCTTTTTAAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattat                                                             <  2:785347/151‑1 (MQ=255)                                                                                       aTCGGCAACTCGGGTGATTTACTTTAGTGGTAATCAGTTTTTTTTTACAACTTTTTGAGAAGAG‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatta                                                              <  1:376825/150‑1 (MQ=255)                                                                                             acctggggTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTTTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                       <  2:1027625/146‑1 (MQ=255)                                                                                             acctggggTGATTTACGTTAGTGGTAATCAGTTTTTTTTAACATTTTTTGGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                        <  1:185593/145‑1 (MQ=255)                                                                                             aaCGCGGGTGATTTCCATTTGGGGTCATGAGTGTTTTTTTACATGTTTTGGAAAAGAA‑AAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                       <  2:79979/151‑1 (MQ=255)                                                                                                 ggggTAATTTACGTTAGGGGTAACCGGGTTTTTTTTACAACTTTTTGAAAAAAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                       <  2:516525/146‑1 (MQ=255)                                                                                                  ggtgaaTTTACGTTGGTGGTTAGCA‑GTTTTTTTTAACACTTTTTTAAAAAAGAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                   <  2:506291/145‑1 (MQ=255)                                                                                                  gggTGATTAACGTTAGTGGTTATCAGTTTTTTTTTACAACTGTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                     <  2:301292/148‑1 (MQ=255)                                                                                                   ggTGATTAACGTGGGGGGTGATCAGGCTTTTTTTACAATTGTTAGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                 <  1:523374/151‑1 (MQ=255)                                                                                                   ggTAATTTACTTTAGTGGTGATCAGGTTTTTTTTACAATTGTTTGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                 <  1:425469/151‑1 (MQ=255)                                                                                                       aTTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGc                                              >  2:106543/1‑150 (MQ=255)                                                                                                        tttATGTTGGTGGTGATCAGGTTTTTTTTACAACTTTTGGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                                <  1:707504/147‑1 (MQ=255)                                                                                                          tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                          >  1:365204/1‑151 (MQ=255)                                                                                                          tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                          >  2:592753/1‑151 (MQ=255)                                                                                                           actttAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAA‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                         <  2:864711/148‑1 (MQ=255)                                                                                                              ttgtgggggATCAGGTTTTTTTTACAATTTTTTGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                        <  1:298679/141‑1 (MQ=255)                                                                                                               tAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                     >  2:395338/1‑151 (MQ=255)                                                                                                                   ggggtccgggttttttttACAACTTTTGAAGAAAAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                   <  1:10508/138‑1 (MQ=255)                                                                                                                     tgATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                               >  2:87039/1‑151 (MQ=255)                                                                                                                     taaTCAGTTTTTTTTAACATTTTTTTAAAAAAAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                 <  1:234918/147‑1 (MQ=255)                                                                                                                       aTCAGTTTTTTTTTACAATTTTTGGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                             <  2:815938/151‑1 (MQ=255)                                                                                                                         cgggttttttttACAACTGTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                           <  2:370625/146‑1 (MQ=255)                                                                                                                          aGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                          >  2:1183890/1‑151 (MQ=255)                                                                                                                           ggttttttttACAATTTTTTGAGAAGAA‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                           <  2:347006/146‑1 (MQ=255)                                                                                                                           ggttttttttAAAACTTTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa                         <  2:896431/148‑1 (MQ=255)                                                                                                                              tttttttACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGt                      >  2:705319/1‑151 (MQ=255)                                                                                                                                tttttACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                     >  1:721406/1‑150 (MQ=255)                                                                                                                                  tttACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                   >  2:984103/1‑150 (MQ=255)                                                                                                                                  tttACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                   >  2:648564/1‑150 (MQ=255)                                                                                                                                   ttACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                 >  2:550968/1‑151 (MQ=255)                                                                                                                                   ttACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                 >  2:476611/1‑151 (MQ=255)                                                                                                                                      caccTGTTTGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                <  2:1049862/146‑1 (MQ=255)                                                                                                                                       aaCTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTc                >  2:1103524/1‑148 (MQ=255)                                                                                                                                           gTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt         >  2:959828/1‑151 (MQ=255)                                                                                                                                            tttGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt        >  1:83903/1‑151 (MQ=255)                                                                                                                                            tttGAGAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc          <  1:167739/149‑1 (MQ=255)                                                                                                                                             ttGAAAAGAA‑AAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt        <  2:646643/150‑1 (MQ=255)                                                                                                                                               gagaagag‑aaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTAACTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc     >  1:553813/1‑151 (MQ=255)                                                                                                                                                  aagaa‑aaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa  <  2:1040822/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAG‑AAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA  >  NC_000913/1824907‑1825202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑