Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,200	0	G	A	44.2%	78.0 / 41.7	77	S217F (TCC→TTC)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base G (34/9);  new base A (0/34);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 7.84e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTT  >  NC_000913/3556051‑3556346                                                                                                                                                      |                                                                                                                                                   ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTTGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAggg                                                                                                                                                    <  1:612424/150‑1 (MQ=255) ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAggg                                                                                                                                                    >  2:541295/1‑150 (MQ=255)   aaCTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGaaa                                                                                                                                                 <  2:292160/151‑1 (MQ=255)      tgtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAAc                                                                                                                                              >  1:372770/1‑151 (MQ=255)       gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACt                                                                                                                                             >  2:468647/1‑151 (MQ=255)         gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTc                                                                                                                                            <  2:1111079/150‑1 (MQ=255)             cAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGc                                                                                                                                       <  1:569994/151‑1 (MQ=255)              aaCCCCCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                      <  2:45334/151‑1 (MQ=255)                ccaccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                      >  2:1181281/1‑149 (MQ=255)                       ggcccggccgcgCTTTTCACCGACGAAAAAAAAGCGTTGGGTGATATTTTCACTTTCGACTCAAAGCGAGACGTTATTCCCCGCCCCCGGGCCGCGCGGCAGTTTATGAATATTCTGTTCAGGCAGGAAAAACCTCCCCGCCAGTGTAg                                                                                                                               <  1:984142/141‑1 (MQ=255)                         caccgcggcccTTTTCACCGAAGAAAAAAAAGCGTTGGGTGATTTTTTCCTTTTCGTCTTCAGGCGAGAGGTTATCCCCCGGCCCCGGGTCGCGCGGCGGGTTATGAATATTTTGTTCAGCCAGAAAAAACCTCCCCGCCAGTGTAGCGAt                                                                                                                           <  1:258659/141‑1 (MQ=255)                         cACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGAt                                                                                                                           >  1:477833/1‑151 (MQ=255)                             gccgcgTTTTCCACCGACGCCAAAAACGCCTTCGGTGTTTTTTCCTCTTTCAATTTAAAGGGAGACGTTTTTCCCCGCCCCCTGTTCCCGCGGCGGTTTATGAATTTTTTGTCCATGCGGGAAAAACCACCCCGCCAGTGTAGCGAttt                                                                                                                         <  1:204136/147‑1 (MQ=255)                                 cgcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                   >  1:1138289/1‑151 (MQ=255)                                  gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                  >  1:238768/1‑151 (MQ=255)                                  gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                  >  1:106381/1‑151 (MQ=255)                                   cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                  >  2:954696/1‑150 (MQ=255)                                    tttcCACCAACGAAAAAAAAGCGTTCGGTGATTTTTTCCCTTTCGCCTCAAAGCGAGACGTTATTCCCCGGCCCTGGGTCGCGCGGCGGTTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCAc                                                                                                                      <  2:598074/145‑1 (MQ=255)                                          ccgccaaaaaaaaaGCTTTGGGTATTTTTTTCCCTTCCGCCTTCAAGGGAGACGTTATCCCCCGGCCCCTGGTCGCGCGGCGGGTTATGAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                           <  2:881696/142‑1 (MQ=255)                                          ccgagaacAAAATCGCTTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGGCGGTATTCCCCGGCCCCTGGGCGCGGGTCAGTTTATAAATTTTCTGTGCATGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                           <  1:803231/144‑1 (MQ=255)                                           cgacgaCAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                         >  1:724680/1‑151 (MQ=255)                                            gacgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                        >  1:980371/1‑151 (MQ=255)                                               gacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc                                                                                                     >  2:1172318/1‑151 (MQ=255)                                                aaaaaaagGCGTTGGGTGATATTTTCCCTTTCGACTTCAAGCGAGCCGGTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATAAATATTCTGTTAATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                      <  2:1028984/147‑1 (MQ=255)                                                aaaaaaaaGCGTTCGGTGATTTTTTCTCTTTCGACTTCAAGCGAGAGGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                    <  1:847930/149‑1 (MQ=255)                                                  aaaaaaGCGTTCGTGTATTTTTACCTTTCCCACTTAAAGCGGGATGTTTTCCCCCGGCCCCTGCTCGCCCGGGGGTTTATAAATATTCTGTTCGTGGGGGGAAAACCCCGCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc                                                                                                       <  1:535123/146‑1 (MQ=255)                                                  aaaaaaGCGTTCGGTAATTTTTTCACTTTCGACTTAAAGCGAGACGTTATTCCCCGCCCCTTGGTCGCGCGGCAGTTTATAAATATTTTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                    <  2:627820/149‑1 (MQ=255)                                                   aaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                                  >  1:745661/1‑150 (MQ=255)                                                     aatgCGTTGGGTGATATTTCCCCTTCCGACTACAAGGGAGGCGGTATTCCCCGCCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                               <  1:171525/148‑1 (MQ=255)                                                      aaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCacac                                                                                              >  1:72729/1‑151 (MQ=255)                                                       aGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                             >  2:1077424/1‑151 (MQ=255)                                                           ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                         >  1:413353/1‑151 (MQ=255)                                                            tCGGTGATATTTTCACTTTCGCCTTCAGGCGAGAGGTTATTCCCCGGCCCCTGGCCGCGCGGCAGTTTATAAATTTTCTTTTAATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                          <  2:415465/149‑1 (MQ=255)                                                            tCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                         >  1:961110/1‑150 (MQ=255)                                                             cGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                        >  2:682147/1‑150 (MQ=255)                                                              ggTGATTTTTTCCTTTTCGACTTCAAGGGGGACGGTATACCCCGCCCCCGGGCCGCGCGGAAGTTTAGAAATTTTTTTTTCTGGAAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                      <  2:686325/151‑1 (MQ=255)                                                              ggTGATTTTTTCACTTTCGACTTCAAGCGAGACGTTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGGGGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                        <  2:347880/149‑1 (MQ=255)                                                              ggTGATATTTTCCCTTCCGCCTTCAAGCGAGACGTTATCCCCCGCCCCCTGGCCGCGCGGCGGTTTATGAATTTTCTGTTAATGAAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                        <  1:413344/149‑1 (MQ=255)                                                              ggTGATATTTTCCCTTCCGACTACAAGCGAGCCGGTATTCCCCGCCCCCGGGGCGCGGGGCGGTTTATAAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                          <  2:497734/147‑1 (MQ=255)                                                              ggTAATTTTTTCCCTTTCGACTCCAAGCGAGAGGTTATCCCCCGCCCCCTGGCCGCGCGGCAGGTTATAAATTTTCTGTCCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                         <  2:22849/148‑1 (MQ=255)                                                                 gATATTTCCACTTTCGACTTCAAGCGAGACGTTATTCCCCGCCCCCTGGCCGCGCGGCAGTTTATGAATTTTTTGTTCATGCAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                     <  1:76974/149‑1 (MQ=255)                                                                    atTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                                >  1:1185490/1‑151 (MQ=255)                                                                      tttCCCTTCCGACTTCAAGCGAGCCGTTATTCCCCGCCCCCGGGTCGCGGGGCAGGTTATGAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt                                                                               <  2:238771/150‑1 (MQ=255)                                                                      tttCCCTTCCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGGCCGCGCGGCAGGTTATAAATATTCTTTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt                                                                               <  2:692793/150‑1 (MQ=255)                                                                         cACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc                                                                           >  1:788389/1‑151 (MQ=255)                                                                          aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc                                                                           >  2:571032/1‑150 (MQ=255)                                                                           cTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCAc                                                                         >  2:567892/1‑151 (MQ=255)                                                                            tttCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtctc                                                                            <  2:442247/147‑1 (MQ=255)                                                                             ttCGACTTCAAGCGAGACGGTATCCCCCGGCCCCTGGCCGCGCGGCAGTTTATAAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCAc                                                                         <  1:105333/149‑1 (MQ=255)                                                                                gactcaaaGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATATTCTTTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc                                                                    <  2:785654/145‑1 (MQ=255)                                                                                    ccAAGCGGGACGGTATTCCCCGCCCCCGGGCCGCGCGGCGGGTTATGAATATTCTTTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc                                                                 <  1:1179712/149‑1 (MQ=255)                                                                                     cAAGCGAGCCGTTATTCCCCGCCCCCGGTCCGCGCGGCAGTTTATAATTATTCTTTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                  <  1:906057/148‑1 (MQ=255)                                                                                        gCGAGACGGTTTTCCCCGGCCCGTGGCCGCGCGGAAGGTTATGAATTTTTTTTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                             <  1:766138/150‑1 (MQ=255)                                                                                        gCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt                                                            >  1:205074/1‑151 (MQ=255)                                                                                           agaggttATTCCCCGCCCCCGGGCCGCGCGGCAGTTTATAAATTTTCTGTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                           <  1:47292/143‑1 (MQ=255)                                                                                           agaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                         >  2:807971/1‑151 (MQ=255)                                                                                               gttATTCCCCGCCCCCTGGCCGCGCGGCGGGTTATAAATATTTTGTCCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                      <  2:372773/148‑1 (MQ=255)                                                                                                gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                    >  2:812045/1‑151 (MQ=255)                                                                                                 tATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                      <  2:1133436/148‑1 (MQ=255)                                                                                                      cccGCCCCCGGGCCGCGCGGCAGGTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                                <  2:213780/149‑1 (MQ=255)                                                                                                           ccctTGGCCGGGCGGCAGATTATGAATTTTTTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                          <  1:588895/150‑1 (MQ=255)                                                                                                               tGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                      >  2:166952/1‑150 (MQ=255)                                                                                                               tGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                      >  2:749483/1‑150 (MQ=255)                                                                                                                  tCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                   >  1:587462/1‑118 (MQ=255)                                                                                                                       cgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                              >  1:1190146/1‑150 (MQ=255)                                                                                                                        ggcggtttATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTaa                            <  2:315087/145‑1 (MQ=255)                                                                                                                        ggcggtttATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTa                             <  2:55353/144‑1 (MQ=255)                                                                                                                        ggCAGGTTATGAATATTCTGTTCATGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTaa                            <  1:438807/151‑1 (MQ=255)                                                                                                                           aGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                         >  2:634281/1‑151 (MQ=255)                                                                                                                              ttATAAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTaaa                           <  2:1165607/146‑1 (MQ=255)                                                                                                                                aTGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                    >  2:717136/1‑151 (MQ=255)                                                                                                                                aTGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                    >  1:630322/1‑151 (MQ=255)                                                                                                                                 tGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                     >  1:378713/1‑149 (MQ=255)                                                                                                                                   aaTTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg                 <  2:810763/151‑1 (MQ=255)                                                                                                                                   aaTTTTCTTTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                    <  1:207544/148‑1 (MQ=255)                                                                                                                                         cTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg             >  2:281316/1‑149 (MQ=255)                                                                                                                                            tccaggaaGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc         <  1:275032/143‑1 (MQ=255)                                                                                                                                               aTGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTc      <  2:508468/150‑1 (MQ=255)                                                                                                                                                  cAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGtt  >  1:1097427/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                   CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTT  >  NC_000913/3556051‑3556346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑