Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,588	A→G	N263D (AAT→GAT)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,588	0	A	G	100.0%	238.4 / NA	65	N263D (AAT→GAT)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/26);  total (39/26)

CAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCT  >  NC_000913/3065443‑3065735                                                                                                                                                  |                                                                                                                                                    cAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATc                                                                                                                                                <  2:519898/151‑1 (MQ=255) cAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATc                                                                                                                                                >  1:331982/1‑151 (MQ=255)  aaCTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCa                                                                                                                                               <  1:972645/151‑1 (MQ=255)       gCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCaataat                                                                                                                                          >  2:197159/1‑151 (MQ=255)       gCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCaataat                                                                                                                                          >  2:713424/1‑151 (MQ=255)        cccGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATg                                                                                                                                         >  1:607466/1‑151 (MQ=255)         ccGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGc                                                                                                                                        >  1:157988/1‑151 (MQ=255)                 ggTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATgg                                                                                                                                <  2:1029888/151‑1 (MQ=255)                  gTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGgc                                                                                                                               <  2:1051914/151‑1 (MQ=255)                    aaTATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGgcgc                                                                                                                             <  2:601914/151‑1 (MQ=255)                     atatGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGcgcg                                                                                                                            >  2:845951/1‑151 (MQ=255)                       atGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGt                                                                                                                           <  2:1151594/150‑1 (MQ=255)                         gCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTg                                                                                                                        >  1:1003409/1‑151 (MQ=255)                          cTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTgg                                                                                                                       >  1:18065/1‑151 (MQ=255)                            ggATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTgggg                                                                                                                     <  1:1156905/151‑1 (MQ=255)                                aaGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGaa                                                                                                                  >  1:482542/1‑150 (MQ=255)                                  gCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAAc                                                                                                               >  2:979422/1‑151 (MQ=255)                                    aaaaTCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAAccc                                                                                                             >  2:792345/1‑151 (MQ=255)                                           cATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAAtt                                                                                                      >  2:528465/1‑151 (MQ=255)                                            aTGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATtc                                                                                                     >  1:941121/1‑151 (MQ=255)                                                gCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATtcctc                                                                                                  <  2:123524/150‑1 (MQ=255)                                                   agcagATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGtt                                                                                              >  2:1022323/1‑151 (MQ=255)                                                    gcagATTGCCGATAACGCGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCCGGGGGAAAACCCGGTAATTCCTCCGctt                                                                                             >  1:823199/1‑151 (MQ=255)                                                        aTTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatga                                                                                         <  1:872811/151‑1 (MQ=255)                                                         ttGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatgat                                                                                        >  1:923615/1‑151 (MQ=255)                                                         ttGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatgat                                                                                        >  2:1111180/1‑151 (MQ=255)                                                          tGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatgatg                                                                                       >  2:703642/1‑151 (MQ=255)                                                               aTAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTAtt                                                                                   >  2:491713/1‑150 (MQ=255)                                                                 aaCGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCg                                                                                 <  1:568422/150‑1 (MQ=255)                                                                    gTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCgg                                                                                >  1:88346/1‑148 (MQ=255)                                                                       gTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTg                                                                           >  2:426358/1‑150 (MQ=255)                                                                         cACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCt                                                                         >  1:822346/1‑150 (MQ=255)                                                                          aCGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCt                                                                         >  1:661907/1‑149 (MQ=255)                                                                                ttATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAAt                                                                 >  2:218397/1‑151 (MQ=255)                                                                                 tATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAAt                                                                 <  1:651031/150‑1 (MQ=255)                                                                                  aTTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCTTTTATGATGTATTCGGAAGTGCTACAGGAATc                                                                <  1:622407/150‑1 (MQ=255)                                                                                  aTTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATc                                                                <  1:301478/150‑1 (MQ=255)                                                                                  aTTGCAGCAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                                                                   <  1:668652/147‑1 (MQ=255)                                                                                   ttGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGGCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCgg                                                              >  1:73227/1‑151 (MQ=255)                                                                                     gCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtg                                                            <  2:506155/151‑1 (MQ=255)                                                                                        ggAAATGGTGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGgtggtg                                                         >  2:1100832/1‑151 (MQ=255)                                                                                              ggCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                    <  2:482545/150‑1 (MQ=255)                                                                                               gcgcATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                    >  1:609628/1‑149 (MQ=255)                                                                                                  cATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACt                                                 >  2:415554/1‑149 (MQ=255)                                                                                                   aTGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTgg                                               >  2:712777/1‑150 (MQ=255)                                                                                                    tGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGa                                              >  2:736589/1‑150 (MQ=255)                                                                                                     gggCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGaa                                             >  2:202119/1‑150 (MQ=255)                                                                                                      ggCGAATTCCGCCGTAATTTCTGCCGCTGCAGAGTGGCGTGGGCGATATCAATAATGCGGTAATTGCGTGTCTGGTGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTCCAGCCATCGCTGGTGCCTTTACtttaa                                             <  1:886460/149‑5 (MQ=255)                                                                                                         gTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCgg                                        <  1:91006/151‑1 (MQ=255)                                                                                                         gTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCgg                                        >  2:484041/1‑151 (MQ=255)                                                                                                           aTTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGc                                       >  2:465769/1‑150 (MQ=255)                                                                                                               cgccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAAt                                  <  1:792334/151‑1 (MQ=255)                                                                                                                gccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGGTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGGGCATTTTCTGGAAACCGGCAAAATc                                 >  1:760788/1‑151 (MQ=255)                                                                                                                 ccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCa                                <  2:1039825/151‑1 (MQ=255)                                                                                                                       tttCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCaaaa                                   <  2:423793/142‑1 (MQ=255)                                                                                                                       tttCTGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCaaaa                                   >  1:423790/1‑142 (MQ=255)                                                                                                                           tGCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGGGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGcc                        >  1:1149409/1‑149 (MQ=255)                                                                                                                            gCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAgc                     <  2:397202/151‑1 (MQ=255)                                                                                                                            gCCGCTGCAAAGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAgc                     <  2:54004/151‑1 (MQ=255)                                                                                                                                   cAAAGTGGCGTGGGCGATATCAATAATGCGGGAATGGCGCGTCTTGGGGAAAACCCGGTAAATCCTCCGGTTATGATGTAATCCGAAGTGCTAAAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCCGCGGGGCCAGCCCCTcc               >  2:376601/1‑150 (MQ=255)                                                                                                                                    aaaGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAg             <  2:137443/151‑1 (MQ=255)                                                                                                                                     aaGTGGCGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGc            <  1:108099/151‑1 (MQ=255)                                                                                                                                           cGTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACa      <  1:83436/151‑1 (MQ=255)                                                                                                                                            gTGGGCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACa      >  1:24699/1‑150 (MQ=255)                                                                                                                                               ggCGATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtct  >  1:314671/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCT  >  NC_000913/3065443‑3065735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑