Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,084,257	T→C	Y552C (TAC→TGC)	speA ←	biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,084,257	0	T	C	100.0%	206.0 / NA	55	Y552C (TAC→TGC)	speA	biosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

GTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACT  >  NC_000913/3084109‑3084404                                                                                                                                                     |                                                                                                                                                    gTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCAc                                                                                                                                                   <  1:360482/151‑1 (MQ=255)  tCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCAc                                                                                                                                                   >  1:236147/1‑150 (MQ=255)    aGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTcc                                                                                                                                                >  2:1163313/1‑151 (MQ=255)                ttctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcattggcatt                                                                                                                                    >  2:841982/1‑151 (MQ=255)                 tctacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCggcattggcat                                                                                                                                     <  2:743669/149‑1 (MQ=255)                   tacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAGACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTc                                                                                                                                 >  1:682263/1‑151 (MQ=255)                   tacGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTc                                                                                                                                 >  1:562416/1‑151 (MQ=255)                    acGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGgcattggcattg                                                                                                                                   <  1:976121/148‑1 (MQ=255)                     cGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGt                                                                                                                               <  2:984411/151‑1 (MQ=255)                       cTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTgg                                                                                                                             >  2:610070/1‑151 (MQ=255)                         aCCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGc                                                                                                                            >  2:124181/1‑150 (MQ=255)                              cAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATAc                                                                                                                       <  1:1176525/150‑1 (MQ=255)                                          acacGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAat                                                                                                          <  2:268054/151‑1 (MQ=255)                                               tCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGt                                                                                                     >  1:434944/1‑151 (MQ=255)                                                  aCCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGt                                                                                                  >  1:45632/1‑151 (MQ=255)                                                     gCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGa                                                                                               <  1:482173/151‑1 (MQ=255)                                                      cTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGa                                                                                               >  1:497144/1‑150 (MQ=255)                                                         cGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAg                                                                                            <  1:519343/150‑1 (MQ=255)                                                                  cGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCaga                                                                                  <  1:1106576/151‑1 (MQ=255)                                                                       aGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTcac                                                                             >  2:51621/1‑151 (MQ=255)                                                                         gTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAg                                                                           <  1:488140/151‑1 (MQ=255)                                                                           tgtgCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAgg                                                                          >  1:991387/1‑150 (MQ=255)                                                                              gCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAat                                                                      <  2:618794/151‑1 (MQ=255)                                                                               cATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAata                                                                     <  1:802213/151‑1 (MQ=255)                                                                                aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                                    <  2:338365/151‑1 (MQ=255)                                                                                aTGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAatat                                                                    >  2:879014/1‑151 (MQ=255)                                                                                  gTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATc                                                                   <  2:1031214/150‑1 (MQ=255)                                                                                       cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCgagcag                                                             <  1:1156822/151‑1 (MQ=255)                                                                                       cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagcag                                                             <  1:841968/151‑1 (MQ=255)                                                                                       cGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCcagcag                                                             <  1:550382/151‑1 (MQ=255)                                                                                        gAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCagcagc                                                            <  1:200679/151‑1 (MQ=255)                                                                                                 cTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGcgcg                                                    >  2:768282/1‑150 (MQ=255)                                                                                                   gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGa                                                   <  1:518/149‑1 (MQ=255)                                                                                                   gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACg                                                 >  2:966359/1‑151 (MQ=255)                                                                                                   gATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGAGTCTCTGGATCGCACTCCGGCAGTGGCATTGTCGTGGCAATACCGCCGCCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACg                                                 <  1:308430/151‑1 (MQ=255)                                                                                                      atGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTc                                              <  2:972279/151‑1 (MQ=255)                                                                                                        gcgcCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCg                                            >  2:832962/1‑151 (MQ=255)                                                                                                             gACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACt                                       >  1:564/1‑151 (MQ=255)                                                                                                              aCCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACtt                                      <  2:650060/151‑1 (MQ=255)                                                                                                                cATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTg                                     >  2:637295/1‑150 (MQ=255)                                                                                                                              gCATCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTcc                        >  2:731910/1‑149 (MQ=255)                                                                                                                                agcggcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGc                     <  1:1158076/148‑1 (MQ=255)                                                                                                                                aTCGGCGGATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCg                    <  1:1059043/151‑1 (MQ=255)                                                                                                                                  cggcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACCGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc                  >  1:989758/1‑151 (MQ=255)                                                                                                                                  cggcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc                  <  2:1139650/151‑1 (MQ=255)                                                                                                                                    gcggATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGc                  >  1:1055522/1‑149 (MQ=255)                                                                                                                                       gATTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAc             >  1:1077715/1‑151 (MQ=255)                                                                                                                                        aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc            >  1:204558/1‑151 (MQ=255)                                                                                                                                        aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc            <  1:525994/151‑1 (MQ=255)                                                                                                                                        aTTCTCTGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAAcc            <  2:1123612/151‑1 (MQ=255)                                                                                                                                           ctctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg         >  2:550821/1‑151 (MQ=255)                                                                                                                                           ctctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg         >  2:326852/1‑151 (MQ=255)                                                                                                                                            tctGGATCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCggg         >  2:565602/1‑150 (MQ=255)                                                                                                                                                 aTCGCACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGaaca     >  1:498370/1‑149 (MQ=255)                                                                                                                                                  tCGCACTCCGGCATTGGCATTGTCGGGGCAATACCGTCACCATCAATATAGTGGTCGATAGAACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACt  >  1:396587/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTCAGACAGTTCTACTTCTACGCTACCGTCAGGGAAGACGAACACGTCAACCGCTTCGGTATCACCGAACAGGTTGTGCATGTTGCCGAGGATCTCCTGATATGCGCCGACCATAAAGAAACCGAGCATCGGCGGATTCTCTGGATCGTACTCCGGCATTGGCATTGTCGTGGCAATACCGTCACCATCAATATAGTGGTCGATAGCACCGTCAGAGTCACAGGTAATATCCAGCAGCACAGCGCGACGTTCCGGCACTTGATCCAGCCCTTCCAGCGGCAGAACCGGGAACAACT  >  NC_000913/3084109‑3084404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑