Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 428,291 C→T S215S (AGC→AGT secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913428,2910CT98.5% 256.4 / NA 67S215S (AGC→AGTsecDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/43);  total (24/43)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATT  >  NC_000913/428143‑428435
                                                                                                                                                    |                                                                                                                                                
ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGa                                                                                                                                                <  1:91487/151‑1 (MQ=255)
        cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGt                                                                                                                                         >  1:513577/1‑150 (MQ=255)
        cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTc                                                                                                                                        >  1:109030/1‑151 (MQ=255)
          tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTg                                                                                                                                      >  2:1095913/1‑151 (MQ=255)
           aaaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTg                                                                                                                                      <  2:630894/150‑1 (MQ=255)
           aaaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTg                                                                                                                                      >  1:735507/1‑150 (MQ=255)
            aaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTg                                                                                                                                      >  1:593572/1‑149 (MQ=255)
            aaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAg                                                                                                                                    <  1:1080580/151‑1 (MQ=255)
            aaGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAg                                                                                                                                    <  1:777648/151‑1 (MQ=255)
               aaaaCAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGa                                                                                                                                 <  1:504590/151‑1 (MQ=255)
               aaaaCAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGa                                                                                                                                 <  1:773729/151‑1 (MQ=255)
                 aacaacTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAg                                                                                                                               <  1:110776/151‑1 (MQ=255)
                   caacTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAgcg                                                                                                                             <  2:322932/151‑1 (MQ=255)
                   caacTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAgc                                                                                                                              <  2:144983/150‑1 (MQ=255)
                        aCGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGa                                                                                                                        <  2:705910/151‑1 (MQ=255)
                          ggCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAat                                                                                                                      >  1:1002998/1‑151 (MQ=255)
                            ccTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAatat                                                                                                                    >  2:753483/1‑151 (MQ=255)
                               gAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGc                                                                                                                  <  1:883990/150‑1 (MQ=255)
                               gAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCg                                                                                                                 <  2:328265/151‑1 (MQ=255)
                                     cACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTgca                                                                                                            <  2:607067/150‑1 (MQ=255)
                                         ttCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGcagcag                                                                                                        <  1:765021/150‑1 (MQ=255)
                                           ccGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGaa                                                                                                      <  1:917184/150‑1 (MQ=255)
                                             gcgATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAAGAt                                                                                                   <  1:794507/151‑1 (MQ=255)
                                                aTGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTaa                                                                                                <  1:747823/151‑1 (MQ=255)
                                                aTGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTa                                                                                                 >  1:633806/1‑150 (MQ=255)
                                                 tGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAat                                                                                               >  1:472644/1‑151 (MQ=255)
                                                  gCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAat                                                                                               <  2:939796/150‑1 (MQ=255)
                                                     aaaGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAatat                                                                                             <  2:906708/149‑1 (MQ=255)
                                                     aaaGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAatat                                                                                             <  2:583061/149‑1 (MQ=255)
                                                     aaaGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATc                                                                                            <  1:918755/150‑1 (MQ=255)
                                                       aGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTg                                                                                         <  1:881350/151‑1 (MQ=255)
                                                           cGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCg                                                                                       <  1:1120785/149‑1 (MQ=255)
                                                            gTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTa                                                                                     >  1:132997/1‑150 (MQ=255)
                                                               atgaAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCg                                                                                 >  1:435793/1‑151 (MQ=255)
                                                                  aaGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCgtgt                                                                              >  2:122232/1‑151 (MQ=255)
                                                                   aGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTa                                                                             <  2:847255/151‑1 (MQ=255)
                                                                       aTTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAAcc                                                                         <  2:370385/151‑1 (MQ=255)
                                                                         tGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCa                                                                        <  2:638888/150‑1 (MQ=255)
                                                                              aTCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtg                                                                   <  1:132976/150‑1 (MQ=255)
                                                                                  gAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggcgtg                                                              <  1:1180858/151‑1 (MQ=255)
                                                                                  gAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggcgtg                                                              <  1:1148784/151‑1 (MQ=255)
                                                                                  gAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggcg                                                                <  2:747404/149‑1 (MQ=255)
                                                                                  gAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggc                                                                 <  1:1071261/148‑1 (MQ=255)
                                                                                    gCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTtggcgtggc                                                            >  2:667392/1‑151 (MQ=255)
                                                                                          gcCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGcc                                                      <  1:141434/151‑1 (MQ=255)
                                                                                          gcCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGcc                                                      >  2:1027182/1‑151 (MQ=255)
                                                                                          gcCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGcc                                                      >  1:269349/1‑151 (MQ=255)
                                                                                           ccATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCg                                                     <  1:354303/151‑1 (MQ=255)
                                                                                             aTCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCggt                                                   <  1:400425/151‑1 (MQ=255)
                                                                                               ccGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCggtgg                                                 >  1:396958/1‑151 (MQ=255)
                                                                                                 ggACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGtt                                               >  2:1126903/1‑151 (MQ=255)
                                                                                                   aCCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGtt                                               >  1:424801/1‑149 (MQ=255)
                                                                                                   aCCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCa                                             <  1:49998/151‑1 (MQ=255)
                                                                                                        gTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTc                                        <  1:942282/151‑1 (MQ=255)
                                                                                                           aTTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAgg                                     >  1:822798/1‑151 (MQ=255)
                                                                                                             tAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAggg                                    <  1:1167048/150‑1 (MQ=255)
                                                                                                                   ccAGGGCAGCAACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGAc                             <  1:334375/151‑1 (MQ=255)
                                                                                                                         cagcaACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATc                       >  1:514916/1‑151 (MQ=255)
                                                                                                                         cagcaACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATc                       >  1:31472/1‑151 (MQ=255)
                                                                                                                            caACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCgtt                    >  1:451519/1‑151 (MQ=255)
                                                                                                                            caACCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCgtt                    <  2:791372/151‑1 (MQ=255)
                                                                                                                              aCCAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCgttgt                  >  2:512044/1‑151 (MQ=255)
                                                                                                                                cAGCTGCGTGCGGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGttgttg                <  2:287462/151‑1 (MQ=255)
                                                                                                                                         gcgGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCa        <  1:263843/150‑1 (MQ=255)
                                                                                                                                          cgGTAATGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATGTCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAgg      <  1:324826/151‑1 (MQ=255)
                                                                                                                                            gTAATGAGCGATGCTCGTCTGAGCGAAGCGCGTGAATATGCGGTGCAGAAGAACATTAATATTCTGCGTAACCGAGTAAAATAACTTGGCGTGGCGGAGCCGGTGGTTCAGAGGCAGGGAGCTGTCCGCATTGTTGTTGAAATGTCAGGt     >  1:757212/1‑150 (MQ=255)
                                                                                                                                              aaTGAGTGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTAtt  <  1:198925/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGGTAATGAGCGATGCTCGTCTGAGTGAAGCGCGTGAATATGCGGTGCAGCAGAACATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATT  >  NC_000913/428143‑428435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: