Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,456	G→A	D286N (GAC→AAC)	wzyE →	putative ECA polysaccharide chain elongation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,456	0	G	A	100.0%	286.7 / NA	72	D286N (GAC→AAC)	wzyE	putative ECA polysaccharide chain elongation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/36);  total (36/36)

TGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTC  >  NC_000913/3979311‑3979601                                                                                                                                                  |                                                                                                                                                  tgttgGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACtt                                                                                                                                               <  1:291112/150‑1 (MQ=255)      gCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAggg                                                                                                                                         >  1:147222/1‑151 (MQ=255)          gTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCt                                                                                                                                      <  2:401418/150‑1 (MQ=255)          gTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTg                                                                                                                                     <  1:936480/151‑1 (MQ=255)            tCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTgg                                                                                                                                    <  2:950328/150‑1 (MQ=255)                   ggcACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAAt                                                                                                                             <  2:588861/150‑1 (MQ=255)                    gcACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTg                                                                                                                           <  2:692386/151‑1 (MQ=255)                    gcACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTg                                                                                                                           >  2:643799/1‑151 (MQ=255)                         aaaaCGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCcg                                                                                                                       <  2:228764/150‑1 (MQ=255)                                tATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCt                                                                                                               >  1:367138/1‑151 (MQ=255)                                   ggAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATg                                                                                                            <  2:1139872/151‑1 (MQ=255)                                   ggAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATg                                                                                                            >  2:802138/1‑151 (MQ=255)                                    gaatgaatGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGt                                                                                                           <  1:911550/151‑1 (MQ=255)                                    gaatgaatGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGt                                                                                                           <  1:70342/151‑1 (MQ=255)                                      atgaatgTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCt                                                                                                         >  1:1105507/1‑151 (MQ=255)                                          atgTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTAt                                                                                                     >  2:708100/1‑151 (MQ=255)                                              gAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATccc                                                                                                  <  2:967920/150‑1 (MQ=255)                                              gAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCt                                                                                                 <  2:635565/151‑1 (MQ=255)                                              gAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCt                                                                                                 >  1:728400/1‑151 (MQ=255)                                               aGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCtt                                                                                                <  2:592875/151‑1 (MQ=255)                                                gcggcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTc                                                                                               <  2:367141/151‑1 (MQ=255)                                                 cggcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTcc                                                                                              >  1:657089/1‑151 (MQ=255)                                                  ggcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTcc                                                                                              <  2:203121/150‑1 (MQ=255)                                                  ggcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCt                                                                                             <  2:268263/151‑1 (MQ=255)                                                   gcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTg                                                                                            >  1:325575/1‑151 (MQ=255)                                                   gcgATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTg                                                                                            <  2:1088695/151‑1 (MQ=255)                                                      aTGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCt                                                                                         <  2:494241/151‑1 (MQ=255)                                                      aTGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCt                                                                                         <  2:1147343/151‑1 (MQ=255)                                                      aTGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCt                                                                                         >  2:97930/1‑151 (MQ=255)                                                      aTGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCt                                                                                         <  1:602307/151‑1 (MQ=255)                                                         aaGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCtgtg                                                                                      <  1:1089575/151‑1 (MQ=255)                                                         aaGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCtgtg                                                                                      >  2:863729/1‑151 (MQ=255)                                                               tCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCggg                                                                                >  2:533058/1‑151 (MQ=255)                                                                 tataCGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTc                                                                              >  2:577646/1‑151 (MQ=255)                                                                   taCGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGt                                                                               <  1:777614/148‑1 (MQ=255)                                                                   taCGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGc                                                                            >  2:407878/1‑151 (MQ=255)                                                                    aCGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTTTTTATCCCTTCCTGGCTGTGGCCGGGTCGcc                                                                           >  1:906670/1‑151 (MQ=255)                                                                     cGTTTCTCTATCTCACTCGCGATACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGccc                                                                          <  1:1025194/151‑1 (MQ=255)                                                                      gTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCg                                                                         >  1:737977/1‑151 (MQ=255)                                                                       tttCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCg                                                                         >  2:68011/1‑150 (MQ=255)                                                                       tttCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCg                                                                         >  1:563254/1‑150 (MQ=255)                                                                         tctcTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGt                                                                      >  2:443018/1‑151 (MQ=255)                                                                          ctctATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGt                                                                      >  1:4579/1‑150 (MQ=255)                                                                          ctctATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGt                                                                      >  1:399906/1‑150 (MQ=255)                                                                             tATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATgg                                                                  >  1:441684/1‑151 (MQ=255)                                                                              aTCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATgg                                                                  <  2:695968/150‑1 (MQ=255)                                                                                 tcACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCt                                                              >  1:1187027/1‑151 (MQ=255)                                                                                  cACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTg                                                             >  2:62319/1‑151 (MQ=255)                                                                                   aCTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTg                                                             >  2:219532/1‑150 (MQ=255)                                                                                      cgcgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGaa                                                           <  1:638137/149‑1 (MQ=255)                                                                                      cgcgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACt                                                         >  1:285991/1‑151 (MQ=255)                                                                                       gcgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTc                                                        >  2:6270/1‑151 (MQ=255)                                                                                        cgACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCa                                                       <  1:370161/151‑1 (MQ=255)                                                                                         gACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTc                                                        >  1:820351/1‑149 (MQ=255)                                                                                                   cGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAactac                                              <  2:403446/149‑1 (MQ=255)                                                                                                    gCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACttt                                           <  1:1131120/151‑1 (MQ=255)                                                                                                     ccGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACttt                                           >  2:143895/1‑150 (MQ=255)                                                                                                      cGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTAc                                         >  1:637387/1‑151 (MQ=255)                                                                                                         gggAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTg                                      >  2:628093/1‑151 (MQ=255)                                                                                                             gaATCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGa                                   >  2:538495/1‑150 (MQ=255)                                                                                                              aaTCTGGCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAg                                 <  2:8530/151‑1 (MQ=255)                                                                                                                   ggCGTTGCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTg                            >  2:77161/1‑151 (MQ=255)                                                                                                                         gCTGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATaa                       <  1:109531/150‑1 (MQ=255)                                                                                                                           tGTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAAcc                     >  2:891629/1‑150 (MQ=255)                                                                                                                            gTTGCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCAc                   >  1:222599/1‑151 (MQ=255)                                                                                                                               gCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTcc                <  2:173597/151‑1 (MQ=255)                                                                                                                               gCAGAACTACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTcc                <  1:876162/151‑1 (MQ=255)                                                                                                                                      tACGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTgg         >  2:31360/1‑151 (MQ=255)                                                                                                                                       aCGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTgg         <  1:349308/150‑1 (MQ=255)                                                                                                                                       aCGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTg          <  2:355297/149‑1 (MQ=255)                                                                                                                                       aCGACAACATCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTg          <  2:290449/149‑1 (MQ=255)                                                                                                                                              caTCAACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGAtctc  <  2:799203/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TGTTGGCATGTTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACGTTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAGAACTACGACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTTATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTC  >  NC_000913/3979311‑3979601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑