Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,204,350 A→G Q344R (CAG→CGG)  zraR → fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,3500AG87.7% 199.0 / ‑1.5 65Q344R (CAG→CGG) zraRfused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (17/40);  minor base C (8/0);  total (25/40)
Fisher's exact test for biased strand distribution p-value = 2.14e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCT  >  NC_000913/4204203‑4204497
                                                                                                                                                    |                                                                                                                                                   
gCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGc                                                                                                                                                  <  2:1013005/151‑1 (MQ=255)
  cAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGa                                                                                                                                                <  1:1083090/151‑1 (MQ=255)
      aTCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCTCTGCTGGG‑TGGCCATTTTTTGCAGCGGCTTGCCGAGCGTAATCGCAAGGCGGGAAAAAGTTTTTCGCCCCCGGCGGTgg                                                                                                                                             >  1:1154551/1‑150 (MQ=255)
          ctactaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGAt                                                                                                                                           <  2:422214/148‑1 (MQ=255)
             ctaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGG‑GGGGCATTTTTTGCAGCGCTTTGCCGAGGGGAAAAGCAAAGGGGGAAAAAGGTTTACGCCCCGGGGGAggggggtggt                                                                                                                                      >  1:584388/1‑143 (MQ=255)
               aTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGAt                                                                                                                                   <  2:858841/151‑1 (MQ=255)
               aTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTAACGCCCCGGGCGATGGATCTGTTg                                                                                                                                     <  1:425816/149‑1 (MQ=255)
                  gCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGATATCCCTCTGCTGGG‑TGGCCATTTTTTGCAGCGCTTTGCCGAGCGTAAACGCAAGGCGGGAAAAGGGTTTTCGCCCCCGGCGATGGGTCTGTTGAt                                                                                                                                   >  2:864008/1‑148 (MQ=255)
                          gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCTCTTCTGGC‑TGGGCCTTTTTTTCAGCGCTTTGTCGGGCGTAATCGCAAAGCGGGGAAAGGGTTTTTGCCCCCGGCGGGGGGTCTGTTGGTTCAtt                                                                                                                              >  1:1004546/1‑145 (MQ=255)
                           tggtggCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCCTTTTTTGCAGCGCTTTTCCCAGCGGAATCCCAAAGCGGGAAAAGGGTTTTCACCCCCGGCGGTGGGTCTGTTTTTTTATTTCGAc                                                                                                                         >  1:61902/1‑149 (MQ=255)
                                 cGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCggg                                                                                                                 <  2:1106782/151‑1 (MQ=255)
                                  gATTGAAGTACCATCGCTGCGGCAACGGCGGGAAAATATTCCCCTTCTGGCTTGGCCCTTTT‑TTCAGCGCTTTTCCCAGCGGTAACGCAAAGCGGGAAAAGGGTTTTAGCCCCCGGCGATGGAGCTGTTGTTTTCTTTCGAACGGCCggg                                                                                                                 >  2:930185/1‑150 (MQ=255)
                                   aTTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGaa                                                                                                               <  1:962713/151‑1 (MQ=255)
                                      gAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAata                                                                                                            <  2:1015993/151‑1 (MQ=255)
                                       aaGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCCCTTCTGGC‑TGGCCCTTTTCTGCCGCGCCTTGCCGAGCGTAAAAGCAAAGCGGTAAAAGGGTTTTCGCCCCCGGCGATGGGTCTGTTGATTCATTACGACTGGCCGGGGAata                                                                                                            >  1:770215/1‑150 (MQ=255)
                                       aaGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAatat                                                                                                           <  2:799599/151‑1 (MQ=255)
                                       aaGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGGAATCGCAAGGCGGGAAAAGGGTTTTCGCCCCCGGCGATGGATCTGTTGATTTATTACGACTGGCCGGGaaa                                                                                                              >  2:124617/1‑148 (MQ=255)
                                         gTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTc                                                                                                         <  1:945452/151‑1 (MQ=255)
                                           aCCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCg                                                                                                        <  1:111490/150‑1 (MQ=255)
                                              aTCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGAc                                                                                                                         <  2:529440/130‑1 (MQ=255)
                                              aTCGCTGCGGCAACGGCGGGAAGAAATCCCTCTGCTGGG‑TGGCCATTTTTTGCCGCGCCTTGGCGAGCGGAATCCCAAAGCGGGAAAAAGGTTTTCGCCCCCGGGGGGGGGGCTGTTGGTTCATTACGAcagatcgg                                                                                                                  >  1:529437/1‑130 (MQ=255)
                                                cGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGc                                                                                                   <  1:286598/150‑1 (MQ=255)
                                                cGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCt                                                                                                  <  1:779911/151‑1 (MQ=255)
                                                   tGCGGCAACGGCGGGAAGAAATCCCTCTTCTGGG‑TGGCCCTTTTCTTCAGCGCTTTGCCGAGCGTAATCGCAAAGCGGTAAAAGGGTTTTCGCCCCGGGCGGTGGGTCTGTTGATTCATTACGACTGGCCGGGGAATATTTGTGAGCTgg                                                                                                >  2:128438/1‑150 (MQ=255)
                                                     cGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGaaa                                                                                             <  2:502961/151‑1 (MQ=255)
                                                      ggCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGa                                                                                               <  1:161965/148‑1 (MQ=255)
                                                        cAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAAcg                                                                                          <  2:1022930/151‑1 (MQ=255)
                                                          aCGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAAcgcg                                                                                        <  2:265963/151‑1 (MQ=255)
                                                                ggAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGa                                                                                   >  1:405699/1‑150 (MQ=255)
                                                                 gAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAAc                                                                                 <  1:568801/151‑1 (MQ=255)
                                                                   aGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACgg                                                                               <  2:68309/151‑1 (MQ=255)
                                                                    gATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACggg                                                                              <  2:845943/151‑1 (MQ=255)
                                                                     atatCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGc                                                                             >  2:262825/1‑151 (MQ=255)
                                                                               gctggc‑tgGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGTCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgctg                                                                   >  2:763150/1‑151 (MQ=255)
                                                                               gctggc‑tgGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggtg                                                                      <  2:459024/148‑1 (MQ=255)
                                                                               gctggc‑tgGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgctg                                                                   <  1:870422/151‑1 (MQ=255)
                                                                                  ggc‑tggcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGTCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTgctgct                                                                 <  2:214664/150‑1 (MQ=255)
                                                                                  ggc‑tggcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGctgctg                                                                <  1:1131393/151‑1 (MQ=255)
                                                                                       ggcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAcc                                                             <  2:746890/150‑1 (MQ=255)
                                                                                       ggcCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCg                                                            <  2:554182/151‑1 (MQ=255)
                                                                                         ccATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCggg                                                          <  1:243968/151‑1 (MQ=255)
                                                                                              ttCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAat                                                      >  1:1011120/1‑150 (MQ=255)
                                                                                                  gCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATAtt                                                 <  1:1093971/151‑1 (MQ=255)
                                                                                                    aGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTc                                               >  1:1017951/1‑151 (MQ=255)
                                                                                                          ttGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGaa                                           <  1:655744/149‑1 (MQ=255)
                                                                                                            gCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAAcgcg                                       <  1:996848/151‑1 (MQ=255)
                                                                                                                aGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCt                                   >  1:910699/1‑151 (MQ=255)
                                                                                                                aGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCt                                   >  2:666941/1‑151 (MQ=255)
                                                                                                                 gCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTg                                  <  1:483880/151‑1 (MQ=255)
                                                                                                                 gCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTg                                  <  2:760419/151‑1 (MQ=255)
                                                                                                                   gTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGcc                                >  1:1056617/1‑151 (MQ=255)
                                                                                                                     aaTCGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATCTCCGAACGCGAGCTGCCg                               <  1:679227/150‑1 (MQ=255)
                                                                                                                        cGCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTgg                           <  1:519574/151‑1 (MQ=255)
                                                                                                                         gCAAGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGc                          >  2:488921/1‑151 (MQ=255)
                                                                                                                            aGGCGGTAAAAGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGAt                       <  1:913009/151‑1 (MQ=255)
                                                                                                                                      aGGTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACg             >  1:218066/1‑151 (MQ=255)
                                                                                                                                        gTTTTACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGcc           <  1:863994/151‑1 (MQ=255)
                                                                                                                                          tttACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCg          >  2:584121/1‑150 (MQ=255)
                                                                                                                                          tttACGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCg          <  1:514398/150‑1 (MQ=255)
                                                                                                                                              cGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATccc     >  2:515638/1‑151 (MQ=255)
                                                                                                                                              cGCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATccc     <  1:211/151‑1 (MQ=255)
                                                                                                                                               gCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCg    >  2:617262/1‑151 (MQ=255)
                                                                                                                                               gCCCCGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCg    >  1:1171844/1‑151 (MQ=255)
                                                                                                                                                 cccGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCt  <  2:61902/151‑1 (MQ=255)
                                                                                                                                                 cccGGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCt  >  2:1104298/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGC‑TGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAGGCGATGGATCTGTTGATTCATTACGACTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCT  >  NC_000913/4204203‑4204497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑