Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,228,771	A→G	E347E (GAA→GAG)	yjbB →	putative Na+/Pi‑cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,228,771	0	A	G	100.0%	307.4 / NA	81	E347E (GAA→GAG)	yjbB	putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/44);  total (37/44)

GTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGG  >  NC_000913/4228627‑4228916                                                                                                                                                 |                                                                                                                                                  gTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAgatg                                                                                                                                             >  1:547634/1‑151 (MQ=255) gTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAgat                                                                                                                                              >  1:603352/1‑150 (MQ=255) gTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAgat                                                                                                                                              >  2:476117/1‑150 (MQ=255)   tttGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAgatgat                                                                                                                                           >  2:138019/1‑151 (MQ=255)   tttGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAgatga                                                                                                                                            <  2:127352/150‑1 (MQ=255)     ttcAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATgg                                                                                                                                         >  2:236129/3‑151 (MQ=255)     tGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGatgatg                                                                                                                                          <  2:195340/150‑1 (MQ=255)          aaCGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAggg                                                                                                                                    >  1:919357/1‑151 (MQ=255)            cGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGtt                                                                                                                                  <  2:819185/151‑1 (MQ=255)              attattCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGtt                                                                                                                                  >  1:984731/1‑149 (MQ=255)                 attCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGaa                                                                                                                               >  1:801866/1‑149 (MQ=255)                 attCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAAt                                                                                                                              >  1:997682/1‑150 (MQ=255)                 attCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAAt                                                                                                                              >  1:890406/1‑150 (MQ=255)                 attCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAAt                                                                                                                              <  1:496831/150‑1 (MQ=255)                    cgcgATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATa                                                                                                                             >  1:1023447/1‑148 (MQ=255)                     gcgATGCCCCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGCTGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATCAAg                                                                                                                          >  1:1175582/1‑150 (MQ=255)                      cgATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTg                                                                                                                        <  2:1166526/151‑1 (MQ=255)                       gATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGa                                                                                                                       <  2:1189166/151‑1 (MQ=255)                         tGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATg                                                                                                                     >  1:916281/1‑151 (MQ=255)                           aaCCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCa                                                                                                                   <  1:1150681/151‑1 (MQ=255)                            aCCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCAc                                                                                                                  >  2:388270/1‑151 (MQ=255)                             ccGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACg                                                                                                                 <  2:712369/151‑1 (MQ=255)                             ccGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACg                                                                                                                 <  2:812405/151‑1 (MQ=255)                                gAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTg                                                                                                              <  1:1093152/151‑1 (MQ=255)                                 aaCTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTg                                                                                                              >  2:560537/1‑150 (MQ=255)                                  aCTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAg                                                                                                            <  1:330735/151‑1 (MQ=255)                                         aCCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACgg                                                                                                      <  1:80727/150‑1 (MQ=255)                                          cccAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCa                                                                                                    <  1:408549/151‑1 (MQ=255)                                           ccAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGc                                                                                                     >  1:1093685/1‑149 (MQ=255)                                            cAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAgg                                                                                                  >  1:225016/1‑151 (MQ=255)                                            cAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAgg                                                                                                  >  1:809094/1‑151 (MQ=255)                                             aGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGga                                                                                                 <  2:53448/151‑1 (MQ=255)                                              gCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGTCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAgg                                                                                                  >  2:909403/1‑149 (MQ=255)                                                 aCGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGaa                                                                                              <  1:1128337/150‑1 (MQ=255)                                                         aaCATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCg                                                                                     >  1:463401/1‑151 (MQ=255)                                                                ggATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                                                               <  2:168949/150‑1 (MQ=255)                                                                ggATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCt                                                                               <  2:483121/150‑1 (MQ=255)                                                                    gTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCa                                                                           <  2:672345/150‑1 (MQ=255)                                                                      cAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgat                                                                        <  1:144316/151‑1 (MQ=255)                                                                      cAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAga                                                                         <  2:365711/150‑1 (MQ=255)                                                                         cgcgcTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAgatgat                                                                     <  1:452276/151‑1 (MQ=255)                                                                           cgcTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGatat                                                                   <  2:1095680/151‑1 (MQ=255)                                                                              tGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCa                                                                 >  1:17416/1‑150 (MQ=255)                                                                               ggATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAGACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                               >  1:756110/1‑151 (MQ=255)                                                                               ggATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                               <  1:178188/151‑1 (MQ=255)                                                                               ggATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAAc                                                               <  1:1172901/151‑1 (MQ=255)                                                                                 aTACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGt                                                             >  1:769321/1‑151 (MQ=255)                                                                                 aTACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGt                                                             >  1:478535/1‑151 (MQ=255)                                                                                         aCGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTac                                                      >  2:1138659/1‑150 (MQ=255)                                                                                         aCGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTac                                                      >  2:733940/1‑150 (MQ=255)                                                                                          cGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTacac                                                    <  2:554984/151‑1 (MQ=255)                                                                                            cTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCg                                                  >  1:16585/1‑151 (MQ=255)                                                                                              tGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGc                                                 <  1:394123/150‑1 (MQ=255)                                                                                               gCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGcc                                                <  1:767688/150‑1 (MQ=255)                                                                                                      cGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCt                                        <  1:92005/151‑1 (MQ=255)                                                                                                      cGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCt                                        >  2:148870/1‑151 (MQ=255)                                                                                                       gAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTg                                       >  1:1107799/1‑151 (MQ=255)                                                                                                       gAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTg                                       <  2:16585/151‑1 (MQ=255)                                                                                                        aaCGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGt                                      <  2:795610/151‑1 (MQ=255)                                                                                                          cgccgcGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTAt                                    >  1:783312/1‑151 (MQ=255)                                                                                                           gccgcGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATc                                   <  2:20419/151‑1 (MQ=255)                                                                                                           gccgcGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATc                                   >  2:763816/1‑151 (MQ=255)                                                                                                            ccgcGCGCGAAACCCTGCGCATTGGGGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCt                                  <  1:902162/151‑1 (MQ=255)                                                                                                            ccgcGCGCGAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCt                                  <  1:849930/151‑1 (MQ=255)                                                                                                             cgcgcgcgAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCt                                  <  2:599536/150‑1 (MQ=255)                                                                                                             cgcgcgcgAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTg                                 <  2:130444/151‑1 (MQ=255)                                                                                                               cgcgcgAAACCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGgc                               <  2:1060439/151‑1 (MQ=255)                                                                                                                     aaaCCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCCCGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCGGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGa                          <  1:924296/150‑1 (MQ=255)                                                                                                                     aaaCCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGCATCTGGCGCGGAt                         <  1:975727/151‑1 (MQ=255)                                                                                                                      aaCCCTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATg                        <  2:131198/151‑1 (MQ=255)                                                                                                                        cccTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCAGATGcc                      >  2:1143112/1‑151 (MQ=255)                                                                                                                          cTGCGCATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCa                     >  1:3440/1‑150 (MQ=255)                                                                                                                            gcgcATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGACCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACTCCGCCATTAAGCTGTATCTGGCGCGGATGCCaaa                   <  2:217070/150‑1 (MQ=255)                                                                                                                               cATTGGCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAaaga                >  1:1137439/1‑150 (MQ=255)                                                                                                                                   ggCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAagag            <  1:66255/150‑1 (MQ=255)                                                                                                                                   ggCGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGc           <  2:1139761/151‑1 (MQ=255)                                                                                                                                     cGACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTg         >  1:143536/1‑151 (MQ=255)                                                                                                                                      gACGCCATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTg         <  2:789308/150‑1 (MQ=255)                                                                                                                                           cATGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCgg     >  2:863102/1‑149 (MQ=255)                                                                                                                                            aTGGAGCAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCggagg  >  2:278328/1‑151 (MQ=255)                                                                                                                                             tGGAGCAGATAATGGAAGGTTTGAATAAAGTGCTGCACGGTGTGCCGCGTCGGGAGAAGGAGCGGCTTAATCTGGCGCGTGATATCAACGTTCTCTCCCCCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCg      <  1:945140/146‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GTTTTGCAAAACGATTATTCGCGATGAACCGGAACTGGATACCCAGCTACGGCCTAAACATCTGGATGTCAGCGCGCTGGATACGCCCACGCTTGCTCTGGCGAACGCCGCGCGCGAAACCCTGCGCATTGGCGACGCCATGGAACAGATGATGGAAGGGTTGAATAAAGTGATGCACGGTGAGCCACGGCAGGAGAAAGAGCTGCGTAAGCTGGCAGATGATATCAACGTTCTCTACACCGCCATTAAGCTGTATCTGGCGCGGATGCCAAAAGAAGAGCTGGCGGAGG  >  NC_000913/4228627‑4228916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑