Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,430,267	A→G	D135G (GAC→GGC)	cycA →	D‑alanine/D‑serine/glycine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,430,267	0	A	G	100.0%	225.6 / NA	60	D135G (GAC→GGC)	cycA	D‑alanine/D‑serine/glycine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/22);  total (38/22)

TTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGT  >  NC_000913/4430121‑4430405                                                                                                                                                   |                                                                                                                                           ttttAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTgg                                                                                                                                        >  1:596045/1‑151 (MQ=255)  tttAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCt                                                                                                                                          <  1:575388/148‑1 (MQ=255)    tAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTc                                                                                                                                     >  1:606885/1‑151 (MQ=255)     aGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCg                                                                                                                                    >  2:46818/1‑151 (MQ=255)       tGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGcc                                                                                                                                  >  1:828968/1‑151 (MQ=255)        gACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCt                                                                                                                                 <  2:252844/151‑1 (MQ=255)        gACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCt                                                                                                                                 >  2:623516/1‑151 (MQ=255)          cTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCg                                                                                                                               >  2:638724/1‑151 (MQ=255)            tCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCt                                                                                                                             >  1:56936/1‑151 (MQ=255)              gCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTgg                                                                                                                           >  1:803687/1‑151 (MQ=255)                  ccGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCgg                                                                                                                        >  1:551272/1‑150 (MQ=255)                   cGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCgggg                                                                                                                      >  2:862199/1‑149 (MQ=255)                    gATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGt                                                                                                                       >  1:852796/1‑149 (MQ=255)                       ttACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGAt                                                                                                                    >  1:254983/1‑149 (MQ=255)                        tACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAg                                                                                                                  <  1:20601/150‑1 (MQ=255)                         aCTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGc                                                                                                                          <  2:287641/141‑1 (MQ=255)                          cTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGtg                                                                                                                >  2:1060137/1‑150 (MQ=255)                          cTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGtg                                                                                                                >  2:296624/1‑150 (MQ=255)                                     ggCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGAc                                                                                                     >  2:1198667/1‑150 (MQ=255)                                     ggCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACg                                                                                                    <  2:687587/151‑1 (MQ=255)                                           atatTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATGGTGCTGCTGCTGACGCTCAAt                                                                                              <  2:649198/151‑1 (MQ=255)                                           atatTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAAt                                                                                              <  2:698561/151‑1 (MQ=255)                                           atatTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGGGATAGGGCTGCTGCTGACGCTCa                                                                                                >  2:1105055/1‑149 (MQ=255)                                            tatTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAAt                                                                                              >  1:1138457/1‑150 (MQ=255)                                                 cACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGGTAGTGCTGCTGCTGACGCTCAATCTCGcc                                                                                        >  1:383425/1‑151 (MQ=255)                                                     ggCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCg                                                                                    >  2:155687/1‑151 (MQ=255)                                                        tGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCg                                                                                    >  2:656318/1‑148 (MQ=255)                                                              tACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATg                                                                            <  1:817177/150‑1 (MQ=255)                                                                 tGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCg                                                                        >  2:483256/1‑151 (MQ=255)                                                                  ggTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTc                                                                         <  2:415642/149‑1 (MQ=255)                                                                   gTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGt                                                                      <  2:594122/151‑1 (MQ=255)                                                                            ggTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATgg                                                               >  2:145826/1‑149 (MQ=255)                                                                             gTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAg                                                             >  2:179051/1‑150 (MQ=255)                                                                               tGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGtt                                                           <  2:11761/150‑1 (MQ=255)                                                                               tGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGtt                                                           >  2:924051/1‑150 (MQ=255)                                                                                gTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCt                                                         <  2:803698/151‑1 (MQ=255)                                                                                   aCCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGt                                                      <  2:232850/151‑1 (MQ=255)                                                                                      ggTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTg                                                   <  2:435560/151‑1 (MQ=255)                                                                                       gTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGc                                                  <  1:98244/151‑1 (MQ=255)                                                                                         aTGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCg                                                 >  1:331316/1‑150 (MQ=255)                                                                                             cAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTTGGTGAGCTGGAGTTCTGGTTTGCgatgat                                            >  1:635373/1‑151 (MQ=255)                                                                                               gACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCa                                          <  1:218108/151‑1 (MQ=255)                                                                                                     gtggCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAAtc                                     >  2:759189/1‑150 (MQ=255)                                                                                                      tggCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGCGAAAATGTTCGGTGGGATGGAGTTCTGGTTTGCGATGATCAAAAtcgt                                   >  1:740957/1‑151 (MQ=255)                                                                                                         cGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGc                                <  1:729776/151‑1 (MQ=255)                                                                                                             cACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCAtt                            >  2:104609/1‑151 (MQ=255)                                                                                                             cACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCAt                             <  2:1145245/150‑1 (MQ=255)                                                                                                                  cTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTgtgt                        >  1:482772/1‑150 (MQ=255)                                                                                                                     aTGCTCAGTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGc                     >  1:339635/1‑150 (MQ=255)                                                                                                                            gTTCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTgtc             <  2:331319/151‑1 (MQ=255)                                                                                                                              tCTGGTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTgtcg            <  2:646927/150‑1 (MQ=255)                                                                                                                                 ggTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCgg        >  2:1096301/1‑151 (MQ=255)                                                                                                                                  gTTCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGc       <  2:621680/151‑1 (MQ=255)                                                                                                                                   ttCCCCGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGcc      >  2:1163155/1‑151 (MQ=255)                                                                                                                                     ccccGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTTATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGGTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCt     >  2:266991/1‑150 (MQ=255)                                                                                                                                     ccccGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGcc      >  2:990471/1‑149 (MQ=255)                                                                                                                                     ccccGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTg    >  2:692909/1‑151 (MQ=255)                                                                                                                                     ccccGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTg    <  2:39227/151‑1 (MQ=255)                                                                                                                                     ccccGATCTCTCCGGCTGGGTCGCCTCGCTAGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTg    >  1:522261/1‑151 (MQ=255)                                                                                                                                       ccGATCTCTCCGGCTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGt  >  2:707227/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                           TTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGT  >  NC_000913/4430121‑4430405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑