Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,492,667	C→T	V229M (GTG→ATG)	idnO ←	5‑keto‑D‑gluconate‑5‑reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,492,667	0	C	T	100.0%	379.0 / NA	93	V229M (GTG→ATG)	idnO	5‑keto‑D‑gluconate‑5‑reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/42);  total (51/42)

GGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATA  >  NC_000913/4492521‑4492812                                                                                                                                                   |                                                                                                                                                  ggCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCgc                                                                                                                                                >  2:672344/1‑150 (MQ=255)   cATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCgcagc                                                                                                                                             >  2:619785/1‑151 (MQ=255)         cTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAAt                                                                                                                                       >  2:1120231/1‑151 (MQ=255)         cTTTATCTCTTATTAGCGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAAt                                                                                                                                       >  2:496240/1‑151 (MQ=255)           ttATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATc                                                                                                                                      >  2:741975/1‑150 (MQ=255)             aTCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGt                                                                                                                                   >  2:1142241/1‑151 (MQ=255)             aTCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGt                                                                                                                                   >  1:65579/1‑151 (MQ=255)                tctTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTcc                                                                                                                                >  2:183559/1‑151 (MQ=255)                 ctTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCt                                                                                                                               >  2:687140/1‑151 (MQ=255)                 ctTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCt                                                                                                                               >  1:281811/1‑151 (MQ=255)                 ctTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCt                                                                                                                               >  1:496545/1‑151 (MQ=255)                  ttattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTg                                                                                                                              >  2:19284/1‑151 (MQ=255)                   tattaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCt                                                                                                                               <  1:780976/149‑1 (MQ=255)                    attaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGc                                                                                                                             <  1:387043/150‑1 (MQ=255)                     ttaGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCgg                                                                                                                           >  1:953856/1‑151 (MQ=255)                         tGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGAtct                                                                                                                       >  1:628260/1‑151 (MQ=255)                           ccAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTcc                                                                                                                     >  1:1132812/1‑151 (MQ=255)                             aGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTcccc                                                                                                                   <  1:710139/151‑1 (MQ=255)                             aGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTcccc                                                                                                                   <  2:908729/151‑1 (MQ=255)                              gAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCa                                                                                                                  <  2:776161/151‑1 (MQ=255)                                 aaGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCg                                                                                                               >  1:350076/1‑151 (MQ=255)                                 aaGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCg                                                                                                               >  2:29466/1‑151 (MQ=255)                                      gcgcTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTgcgg                                                                                                          <  2:860711/151‑1 (MQ=255)                                      gcgcTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTgcgg                                                                                                          <  1:786605/151‑1 (MQ=255)                                       cgcTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCA‑CAATCAGTTCCTGCGGATCTCCCCAGCGTgcggcg                                                                                                        <  2:268159/151‑1 (MQ=255)                                           ttCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGcggcgg                                                                                                       <  2:214477/149‑1 (MQ=255)                                           ttCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCggg                                                                                                      <  2:324240/150‑1 (MQ=255)                                            tCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGCGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCggggg                                                                                                    >  2:114738/1‑151 (MQ=255)                                             cgcgCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCggggg                                                                                                    <  1:960189/150‑1 (MQ=255)                                               cgcTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTcc                                                                                                 >  1:1075395/1‑151 (MQ=255)                                               cgcTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCACAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTcc                                                                                                 >  2:176173/1‑151 (MQ=255)                                                     aCGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGc                                                                                           >  1:574328/1‑151 (MQ=255)                                                                 tGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCgg                                                                               >  2:229732/1‑151 (MQ=255)                                                                 tGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCgg                                                                               >  2:158749/1‑151 (MQ=255)                                                                  gTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCgg                                                                               >  2:393900/1‑150 (MQ=255)                                                                  gTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCg                                                                                <  2:1087176/149‑1 (MQ=255)                                                                  gTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGt                                                                              <  1:649426/151‑1 (MQ=255)                                                                   ttAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTg                                                                             >  2:445621/1‑151 (MQ=255)                                                                     aaaCAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGa                                                                            <  1:685640/150‑1 (MQ=255)                                                                       aCAGCCACTAACATGCCCCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAgg                                                                         <  2:688026/151‑1 (MQ=255)                                                                        cAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGc                                                                        <  1:1200473/151‑1 (MQ=255)                                                                         aGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGGGaa                                                                           >  2:170879/1‑147 (MQ=255)                                                                           ccACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCtt                                                                      >  2:969759/1‑150 (MQ=255)                                                                           ccACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTc                                                                     <  1:20695/151‑1 (MQ=255)                                                                           ccACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTc                                                                     <  2:135121/151‑1 (MQ=255)                                                                            cACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCg                                                                    <  2:123786/151‑1 (MQ=255)                                                                               tAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGCCCGTTTGCACAACCAGGCGGGGAAGGCCTcttac                                                                 >  1:866359/1‑147 (MQ=255)                                                                                aaCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTcc                                                                 >  1:322512/1‑150 (MQ=255)                                                                                aaCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTcc                                                                 <  1:189245/150‑1 (MQ=255)                                                                                 aCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCggggg                                                                                                    <  2:632827/114‑1 (MQ=255)                                                                                 aCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCggggg                                                                                                    >  1:632818/1‑114 (MQ=255)                                                                                 aCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCCGGCGGTGAAGGCTTCg                                                                    >  2:1119889/1‑146 (MQ=255)                                                                                 aCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAAGCTTCGTCCTc                                                               >  2:736213/1‑151 (MQ=255)                                                                                 aCATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAAGCCTCGTCCt                                                                >  1:948653/1‑150 (MQ=255)                                                                                   aTGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTc                                                               >  2:493936/1‑149 (MQ=255)                                                                                     gccgccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAAcc                                                           <  2:25931/151‑1 (MQ=255)                                                                                     gccgccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAAcc                                                           <  2:359400/151‑1 (MQ=255)                                                                                     gccgccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAAc                                                            <  2:337634/150‑1 (MQ=255)                                                                                       cgccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAg                                                         <  2:399005/151‑1 (MQ=255)                                                                                        gccATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGt                                                        <  2:66584/151‑1 (MQ=255)                                                                                        gccATCAACAAACAACAGGTGGCCG‑TTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTg                                                       <  1:373295/151‑1 (MQ=255)                                                                                           aTCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCt                                                     >  2:853290/1‑151 (MQ=255)                                                                                            tCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCtt                                                    >  2:1151982/1‑151 (MQ=255)                                                                                                    caacaGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAAACAGGGCTTTAGTCAtt                                            >  2:504201/1‑151 (MQ=255)                                                                                                     aacaGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCAttt                                           <  2:65579/151‑1 (MQ=255)                                                                                                     aacaGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCAttt                                           <  1:1133210/151‑1 (MQ=255)                                                                                                            ggCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTTCGGCGTGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGttt                                     <  2:1098458/150‑1 (MQ=255)                                                                                                            ggCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGtttt                                    >  2:1077406/1‑151 (MQ=255)                                                                                                            ggCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGtttt                                    <  2:868744/151‑1 (MQ=255)                                                                                                               cGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGaa                                 <  2:475465/151‑1 (MQ=255)                                                                                                               cGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGGGCTTTAGTCATTTCTGtttt                                    >  1:751745/1‑148 (MQ=255)                                                                                                                gTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGCGAAGGCCTCGTCCTCCACCCGGGGTTTTGTCATTTCTGtttt                                    >  1:1046857/1‑147 (MQ=255)                                                                                                                 tttACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGaaa                                >  2:1034119/1‑150 (MQ=255)                                                                                                                  ttACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAAt                               <  1:189124/150‑1 (MQ=255)                                                                                                                   tACGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATa                              <  1:262521/150‑1 (MQ=255)                                                                                                                     cGAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGcc                           <  2:712189/151‑1 (MQ=255)                                                                                                                      gAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTTGTCATTTTTGTTTTTAAATAAccc                          >  1:759657/1‑151 (MQ=255)                                                                                                                      gAAATCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGccc                          >  2:500650/1‑151 (MQ=255)                                                                                                                          tCAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCCGTGCTTTAGTCATTTCTGTTTTGAAATAACCCgg                        >  1:1168176/1‑149 (MQ=255)                                                                                                                           cAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCCCTAACAAGGCTTTAGTCATTTCTGATTTGAAATAGccc                          >  1:202563/1‑146 (MQ=255)                                                                                                                           cAGAGGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGGGAAGGCTTCGTCCTCAACCAGTGCTTTTGTCATTTCTGTTTTGAAATAGCCCgg                        >  1:1193706/1‑148 (MQ=255)                                                                                                                             gagGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCaa                   <  1:945482/151‑1 (MQ=255)                                                                                                                              agGCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCaa                   >  2:126329/1‑150 (MQ=255)                                                                                                                               ggCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAAt                  >  1:84562/1‑150 (MQ=255)                                                                                                                                gCTTTTGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGGTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGgcgc                     >  2:1110716/1‑146 (MQ=255)                                                                                                                                   tttGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATAcc               <  2:357521/149‑1 (MQ=255)                                                                                                                                   tttGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGt             >  2:224441/1‑151 (MQ=255)                                                                                                                                    ttGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGt             <  2:628269/150‑1 (MQ=255)                                                                                                                                     tGAAGAAAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGtt            <  2:677120/150‑1 (MQ=255)                                                                                                                                      gaagaaAGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTg           <  1:393897/150‑1 (MQ=255)                                                                                                                                            aGGAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGCGCAGGCTTCGTCCCCAAACAGGGCTTTAGGCATTTCCTTTTTGAAATAGCCCGGCGCAAGACCGGTGACCCGa     >  1:796858/1‑150 (MQ=255)                                                                                                                                             ggAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAat   <  1:1111968/151‑1 (MQ=255)                                                                                                                                              gAACATCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAata  <  1:608052/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GGCATAGTCTTTATCTCTTATTAGTGCCAGAAAAGCAGCGCTTTCGCGCTGCACGTAGGGGGGCTGTTAAACAGCCACTAACATGCCGCCATCAACAAACAACAGGTGGCCGTTTACGAAATCAGAGGCTTTTGAAGAAAGGAACACCGCAGCACCAATCAGTTCCTGCGGATCTCCCCAGCGTGCGGCGGGGGTCCGTTTGCACAACCAGGCGGTGAAGGCTTCGTCCTCAACCAGTGCTTTAGTCATTTCTGTTTTGAAATAGCCCGGCGCAATACCGTTGACCTGAATA  >  NC_000913/4492521‑4492812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑